BLASTX nr result
ID: Dioscorea21_contig00005646
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00005646
(1738 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI39064.3| unnamed protein product [Vitis vinifera] 456 e-142
ref|XP_002265256.2| PREDICTED: nucleolar GTP-binding protein 1-l... 456 e-142
ref|XP_002526509.1| nucleolar GTP-binding protein, putative [Ric... 432 e-135
ref|XP_002302481.1| predicted protein [Populus trichocarpa] gi|2... 449 e-134
ref|XP_002889273.1| PDE318 [Arabidopsis lyrata subsp. lyrata] gi... 427 e-132
>emb|CBI39064.3| unnamed protein product [Vitis vinifera]
Length = 456
Score = 456 bits (1173), Expect(2) = e-142
Identities = 223/309 (72%), Positives = 267/309 (86%), Gaps = 7/309 (2%)
Frame = -3
Query: 1316 ELAVPLRQYTENFPNKQYLHPYERSLIELTLGDGNYEEVLGRVDSLRKKIVSIGKQQASL 1137
ELAVPLR Y ENFP+K+ LHPYE+SLI+LTLGDGNYE+VL RVDSLRKK+VS+GK+ ASL
Sbjct: 135 ELAVPLRGYAENFPDKKQLHPYEQSLIQLTLGDGNYEQVLRRVDSLRKKVVSVGKEHASL 194
Query: 1136 CAQSMSKREAEERLNEGLKKIEEVFHIGSNAVEDLLHVAKTLRAMPVVDLEKPTLCLVGA 957
CA+S++KREAEERL EG+KK+E++F+ AV+DLL++AKTLRAMPVVDLE PTLCLVGA
Sbjct: 195 CAKSLTKREAEERLGEGMKKLEDIFNHEGKAVDDLLNIAKTLRAMPVVDLETPTLCLVGA 254
Query: 956 PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHIILNYERFQVTDTPGLLTRHDEDRNNL 777
PNVGKSSLVR+LSTGKPEVCNYPFTTRGILMGHI NY FQVTDTPGLL RHD++RNNL
Sbjct: 255 PNVGKSSLVRVLSTGKPEVCNYPFTTRGILMGHIAFNYRHFQVTDTPGLLNRHDDERNNL 314
Query: 776 ERLTLAVLSHLPTAVLYVHDLSGECGTSPDDQFTTYKEMKERFSNHLWLDVVSKCDLLQV 597
E+LTLAVLSHLPTA+LYVHDLSGECGTSP DQF YKE++ERFS+HLW+DVVSKCDLLQ
Sbjct: 315 EKLTLAVLSHLPTAILYVHDLSGECGTSPSDQFFIYKEIRERFSDHLWIDVVSKCDLLQE 374
Query: 596 SP-ASNLED------DLEKYKSFGPDGAIRVSVKSEFGLPELKERVHDMLISQMAEIRNE 438
SP N ED +L +Y+ GP+GAI VSVK+E L +LK RVH++L+SQM+ I++
Sbjct: 375 SPIIFNTEDVSADNPELARYQKMGPEGAIHVSVKNEAALNQLKGRVHELLVSQMSRIKSS 434
Query: 437 TNSKES*GI 411
+++E+ GI
Sbjct: 435 ESNEENKGI 443
Score = 77.4 bits (189), Expect(2) = e-142
Identities = 46/128 (35%), Positives = 72/128 (56%), Gaps = 2/128 (1%)
Frame = -2
Query: 1725 TFNLLQQWRIPPGQSLSRRRE--RLGYQILKGHFFPVKVWNCRSFCCCQELQSNVGDVVE 1552
T +L+Q W IP + L R + Y + +P K++ C+++Q+ +V
Sbjct: 4 TSHLIQLWHIPSTKLLLRSSNFSKGSYLVPAAEIYPTKIF-------CKKIQTTAYRIVN 56
Query: 1551 KSSAFGSDIALKIGTQGKPAQNHKSLGAFQKLPVVMPSIDILASAKRKARNVPPSRGIAN 1372
S + + + +++GAFQKLP+VMPS+DIL SA RKA+ V P++GIAN
Sbjct: 57 GSYVPVPQNKNQSNKEDIASTTLETVGAFQKLPMVMPSVDILYSALRKAKRVSPTKGIAN 116
Query: 1371 LAKRERNK 1348
+AKRERN+
Sbjct: 117 IAKRERNR 124
>ref|XP_002265256.2| PREDICTED: nucleolar GTP-binding protein 1-like [Vitis vinifera]
Length = 445
Score = 456 bits (1172), Expect(2) = e-142
Identities = 222/309 (71%), Positives = 267/309 (86%), Gaps = 7/309 (2%)
Frame = -3
Query: 1316 ELAVPLRQYTENFPNKQYLHPYERSLIELTLGDGNYEEVLGRVDSLRKKIVSIGKQQASL 1137
ELAVPLR Y ENFP+K+ LHPYE+SLI+LTLGDGNYE+VL RVDSLRKK+VS+GK+ ASL
Sbjct: 135 ELAVPLRGYAENFPDKKQLHPYEQSLIQLTLGDGNYEQVLRRVDSLRKKVVSVGKEHASL 194
Query: 1136 CAQSMSKREAEERLNEGLKKIEEVFHIGSNAVEDLLHVAKTLRAMPVVDLEKPTLCLVGA 957
CA+S++KREAEERL EG+KK+E++F+ AV+DLL++AKTLRAMPVVDLE PTLCLVGA
Sbjct: 195 CAKSLTKREAEERLGEGMKKLEDIFNHEGKAVDDLLNIAKTLRAMPVVDLETPTLCLVGA 254
Query: 956 PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHIILNYERFQVTDTPGLLTRHDEDRNNL 777
PNVGKSSLVR+LSTGKPEVCNYPFTTRGILMGHI NY FQVTDTPGLL RHD++RNNL
Sbjct: 255 PNVGKSSLVRVLSTGKPEVCNYPFTTRGILMGHIAFNYRHFQVTDTPGLLNRHDDERNNL 314
Query: 776 ERLTLAVLSHLPTAVLYVHDLSGECGTSPDDQFTTYKEMKERFSNHLWLDVVSKCDLLQV 597
E+LTLAVLSHLPTA+LYVHDLSGECGTSP DQF YKE++ERFS+HLW+DVVSKCDLLQ
Sbjct: 315 EKLTLAVLSHLPTAILYVHDLSGECGTSPSDQFFIYKEIRERFSDHLWIDVVSKCDLLQE 374
Query: 596 SP-ASNLED------DLEKYKSFGPDGAIRVSVKSEFGLPELKERVHDMLISQMAEIRNE 438
SP N ED +L +Y+ GP+GAI VSVK+E L +LK RVH++L+SQM+ I++
Sbjct: 375 SPIIFNTEDVSADNPELARYQKMGPEGAIHVSVKNEAALNQLKGRVHELLVSQMSRIKSS 434
Query: 437 TNSKES*GI 411
+++E+ G+
Sbjct: 435 ESNEENKGV 443
Score = 77.4 bits (189), Expect(2) = e-142
Identities = 46/128 (35%), Positives = 72/128 (56%), Gaps = 2/128 (1%)
Frame = -2
Query: 1725 TFNLLQQWRIPPGQSLSRRRE--RLGYQILKGHFFPVKVWNCRSFCCCQELQSNVGDVVE 1552
T +L+Q W IP + L R + Y + +P K++ C+++Q+ +V
Sbjct: 4 TSHLIQLWHIPSTKLLLRSSNFSKGSYLVPAAEIYPTKIF-------CKKIQTTAYRIVN 56
Query: 1551 KSSAFGSDIALKIGTQGKPAQNHKSLGAFQKLPVVMPSIDILASAKRKARNVPPSRGIAN 1372
S + + + +++GAFQKLP+VMPS+DIL SA RKA+ V P++GIAN
Sbjct: 57 GSYVPVPQNKNQSNKEDIASTTLETVGAFQKLPMVMPSVDILYSALRKAKRVSPTKGIAN 116
Query: 1371 LAKRERNK 1348
+AKRERN+
Sbjct: 117 IAKRERNR 124
>ref|XP_002526509.1| nucleolar GTP-binding protein, putative [Ricinus communis]
gi|223534184|gb|EEF35900.1| nucleolar GTP-binding
protein, putative [Ricinus communis]
Length = 443
Score = 432 bits (1111), Expect(2) = e-135
Identities = 213/306 (69%), Positives = 257/306 (83%), Gaps = 7/306 (2%)
Frame = -3
Query: 1316 ELAVPLRQYTENFPNKQYLHPYERSLIELTLGDGNYEEVLGRVDSLRKKIVSIGKQQASL 1137
ELA+PLR+Y ENFP ++YLHPYE+SLIELTLGDGNYEEVL +VD+LRKK+VSIGK+ AS+
Sbjct: 137 ELALPLREYLENFPKRKYLHPYEQSLIELTLGDGNYEEVLRKVDALRKKVVSIGKEHASV 196
Query: 1136 CAQSMSKREAEERLNEGLKKIEEVFHIGSNAVEDLLHVAKTLRAMPVVDLEKPTLCLVGA 957
CA+S++KREAEE+LNEG +K+EE+F AV+DLL++AKTLRAMPVV+LE PTLCLVGA
Sbjct: 197 CAKSLTKREAEEQLNEGREKLEEIFKCEGKAVDDLLNIAKTLRAMPVVNLETPTLCLVGA 256
Query: 956 PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHIILNYERFQVTDTPGLLTRHDEDRNNL 777
PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHI +++ FQVTDTPGLL R DEDRNNL
Sbjct: 257 PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHIAFDFQHFQVTDTPGLLKRCDEDRNNL 316
Query: 776 ERLTLAVLSHLPTAVLYVHDLSGECGTSPDDQFTTYKEMKERFSNHLWLDVVSKCDLLQV 597
E+LTLAVL+HLPTA+LYVHDL+GECGTS DQF YKE+KERFS+HLWLDVVSKCDLLQ
Sbjct: 317 EKLTLAVLTHLPTAILYVHDLTGECGTSASDQFVIYKEIKERFSDHLWLDVVSKCDLLQE 376
Query: 596 SPA-------SNLEDDLEKYKSFGPDGAIRVSVKSEFGLPELKERVHDMLISQMAEIRNE 438
S +N ++ +Y GP+GAIRVS+KS GL ELK RV ++L+ QM I++
Sbjct: 377 SSMIFNAKDNNNDHFEMVRYHKVGPNGAIRVSIKSGEGLNELKGRVQNLLVGQMERIKSM 436
Query: 437 TNSKES 420
+ ++
Sbjct: 437 ASKDDN 442
Score = 79.0 bits (193), Expect(2) = e-135
Identities = 49/128 (38%), Positives = 69/128 (53%), Gaps = 1/128 (0%)
Frame = -2
Query: 1725 TFNLLQQWRIPPGQSLSRRRERLGYQILKGHFFPVKVWNCRSFCCCQELQSNVGDVVEKS 1546
+ +LLQ IP R +G + +V C + C + Q+ ++V S
Sbjct: 4 SLSLLQLRHIPSANKFLFRSSNF----CQGLYMNARVSQCPNLCFSRHTQTITYEIVNAS 59
Query: 1545 SA-FGSDIALKIGTQGKPAQNHKSLGAFQKLPVVMPSIDILASAKRKARNVPPSRGIANL 1369
+ T K N ++LGAFQKLP+VMPS+DIL SA RKA+ V P++GIAN+
Sbjct: 60 YVPIHQTKPTRSYTTDKSPGNLQNLGAFQKLPMVMPSVDILYSALRKAKRVSPTKGIANI 119
Query: 1368 AKRERNKG 1345
AKRERN+G
Sbjct: 120 AKRERNRG 127
>ref|XP_002302481.1| predicted protein [Populus trichocarpa] gi|222844207|gb|EEE81754.1|
predicted protein [Populus trichocarpa]
Length = 356
Score = 449 bits (1154), Expect(2) = e-134
Identities = 221/299 (73%), Positives = 257/299 (85%), Gaps = 7/299 (2%)
Frame = -3
Query: 1316 ELAVPLRQYTENFPNKQYLHPYERSLIELTLGDGNYEEVLGRVDSLRKKIVSIGKQQASL 1137
ELAVPLR+Y ENFP K+YLHPYERSLIELTLGDGNYEEVL +VD+LRKK+V +GK+ ASL
Sbjct: 46 ELAVPLREYMENFPKKKYLHPYERSLIELTLGDGNYEEVLRKVDALRKKVVGVGKECASL 105
Query: 1136 CAQSMSKREAEERLNEGLKKIEEVFHIGSNAVEDLLHVAKTLRAMPVVDLEKPTLCLVGA 957
CA+S +KR+A ERLNEGL+K+EEVF AV+DLL++AKTLRAMPVVDLE PTLCLVGA
Sbjct: 106 CAKSSTKRDAGERLNEGLEKLEEVFKCEGKAVDDLLNIAKTLRAMPVVDLETPTLCLVGA 165
Query: 956 PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHIILNYERFQVTDTPGLLTRHDEDRNNL 777
PNVGKSSLVR+LSTGKPEVCNYPFTTRGILMGHI LN++ FQVTDTPGLL R DEDRNNL
Sbjct: 166 PNVGKSSLVRVLSTGKPEVCNYPFTTRGILMGHIALNFQHFQVTDTPGLLKRCDEDRNNL 225
Query: 776 ERLTLAVLSHLPTAVLYVHDLSGECGTSPDDQFTTYKEMKERFSNHLWLDVVSKCDLLQV 597
E+LTLAVLSHLPTA+LYVHDL+GECGTSP DQF YKE+K+RFSNHLWLDVVSKCD+LQ
Sbjct: 226 EKLTLAVLSHLPTAILYVHDLTGECGTSPSDQFVIYKEIKQRFSNHLWLDVVSKCDILQE 285
Query: 596 SPASNLEDD-------LEKYKSFGPDGAIRVSVKSEFGLPELKERVHDMLISQMAEIRN 441
+P + +D LE+Y+ GP+GAI VSVK GL ELK +VH++L++QM IR+
Sbjct: 286 APVIFIMEDSDTDNPELERYRKKGPEGAILVSVKDGKGLDELKVQVHELLVTQMDRIRS 344
Score = 58.2 bits (139), Expect(2) = e-134
Identities = 26/36 (72%), Positives = 33/36 (91%)
Frame = -2
Query: 1452 VVMPSIDILASAKRKARNVPPSRGIANLAKRERNKG 1345
+VMPS+DIL SA RKA+ VPP++GIAN+AKRERN+G
Sbjct: 1 MVMPSVDILYSALRKAKRVPPTKGIANIAKRERNRG 36
>ref|XP_002889273.1| PDE318 [Arabidopsis lyrata subsp. lyrata] gi|297335114|gb|EFH65532.1|
PDE318 [Arabidopsis lyrata subsp. lyrata]
Length = 449
Score = 427 bits (1099), Expect(2) = e-132
Identities = 212/308 (68%), Positives = 255/308 (82%), Gaps = 7/308 (2%)
Frame = -3
Query: 1316 ELAVPLRQYTENFPNKQYLHPYERSLIELTLGDGNYEEVLGRVDSLRKKIVSIGKQQASL 1137
ELA+PL+ Y E+FP K+ LHPYERSLI+LTLGDG YEEVLG+VD LRKK++S+GK+ ASL
Sbjct: 142 ELALPLKGYMESFPRKRLLHPYERSLIDLTLGDGKYEEVLGKVDVLRKKVLSVGKEHASL 201
Query: 1136 CAQSMSKREAEERLNEGLKKIEEVFHIGSNAVEDLLHVAKTLRAMPVVDLEKPTLCLVGA 957
CA+++SK+EAEERLNEG++K+E VF AV+DLL +AK LRAMPVVDLE PTLCLVGA
Sbjct: 202 CAKALSKKEAEERLNEGVEKLELVFQQQGRAVDDLLSIAKVLRAMPVVDLEMPTLCLVGA 261
Query: 956 PNVGKSSLVRILSTGKPEVCNYPFTTRGILMGHIILNYERFQVTDTPGLLTRHDEDRNNL 777
PNVGKSSLVRILSTGKPE+CNYPFTTRGILMGHI+LNY+RFQVTDTPGLL R DEDRNNL
Sbjct: 262 PNVGKSSLVRILSTGKPEICNYPFTTRGILMGHIVLNYQRFQVTDTPGLLRRCDEDRNNL 321
Query: 776 ERLTLAVLSHLPTAVLYVHDLSGECGTSPDDQFTTYKEMKERFSNHLWLDVVSKCDLLQV 597
E+LTLAVL+HLPTAVLYVHDL+GECGTSP DQF YKEMKERF ++LW+D VSKCDLL
Sbjct: 322 EKLTLAVLTHLPTAVLYVHDLTGECGTSPSDQFRIYKEMKERFKDYLWIDAVSKCDLLGG 381
Query: 596 SPASNLEDD-------LEKYKSFGPDGAIRVSVKSEFGLPELKERVHDMLISQMAEIRNE 438
SP ++D + KY+ GPD +I VSVK+E GL ELK +V ++L ++M +I++
Sbjct: 382 SPVMYAKEDRSSDDAEIIKYRETGPDESIHVSVKTEQGLNELKSKVKEVLSNEMEKIKSG 441
Query: 437 TNSKES*G 414
+S G
Sbjct: 442 EKIDQSVG 449
Score = 73.9 bits (180), Expect(2) = e-132
Identities = 42/89 (47%), Positives = 60/89 (67%), Gaps = 7/89 (7%)
Frame = -2
Query: 1590 CQELQSNVGDVVEKS---SAFGSDIALKIGT----QGKPAQNHKSLGAFQKLPVVMPSID 1432
C+ LQ+ V +V S +++ + ++I T Q P Q + LGAFQKLP+VMPSID
Sbjct: 46 CRNLQTAVSPIVSSSYLPTSYITQKQIEIPTSPEKQSPPVQ--EGLGAFQKLPMVMPSID 103
Query: 1431 ILASAKRKARNVPPSRGIANLAKRERNKG 1345
+ SA RK++ V P++GIAN+AKRERN+G
Sbjct: 104 LYCSALRKSKRVQPTKGIANIAKRERNRG 132