BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00005510
         (1721 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera]             700   0.0  
ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinife...   700   0.0  
ref|XP_003554965.1| PREDICTED: cytochrome P450 85A-like [Glycine...   691   0.0  
ref|XP_003544008.1| PREDICTED: cytochrome P450 85A-like [Glycine...   688   0.0  
gb|ACR20476.1| steroid C-6 oxidase [Gossypium hirsutum]               687   0.0  

>gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera]
          Length = 460

 Score =  700 bits (1806), Expect = 0.0
 Identities = 336/447 (75%), Positives = 382/447 (85%)
 Frame = +1

Query: 208  SAMLRWNELRYRKKGLPPGTMGWPLFGETTEFLKQGPSFLKNQRARYGSLFKSHILGCPT 387
            +A+LRWNE+RYRKKGLPPGTMGWP+FGETTEFLKQGPSF+KNQRARYG  FKSH+LGCPT
Sbjct: 15   TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 74

Query: 388  VVSMDPELNRFILMNEGKGLVPGYPQSMLDILGKCNIAAVDGPLHKAMRGAMLGLINPPM 567
             VSMDPELNR+ILMNE KGLVPGYPQSMLDILGKCNIAAV G  HK MRGA+L LI+P M
Sbjct: 75   TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 134

Query: 568  IRDQLLHKIDQFMRSHLTDWSNKIIDIQQKTKEMALLSALKQIASIETGSFAESLKTEIF 747
            IR QLL KID+FMRSHL  W  KII+IQ+KTKEMALLS+LKQIA IE+G+ ++    E F
Sbjct: 135  IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEFMPEFF 194

Query: 748  KLVVGTLSLPINLPGTNYRRGLQARKKAVGMLRDLIQQRRESNCSQNDMLDCLLKNDGST 927
            KLV+GT+SLPI+LPGTNYRRG QARK  VGMLR LI++R+ S  + NDML CL++ +   
Sbjct: 195  KLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTN-EN 253

Query: 928  RSMLSDDQIIDLIIALVYSGYETVSTTSMMAVKYLLDHPKALEELRKEHFGIRNGKASEE 1107
            R  LSD++IIDLII ++YSGYETVSTTSMMAVKYL DHP+ L+ELRKEH  IR  K  E+
Sbjct: 254  RYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRPED 313

Query: 1108 GIDWNDYKSMRFTRAVILETLRVAAVVNGVLRKTTHDMEMKGFLIPKGWRIYVYTREINY 1287
             IDWNDYK MRFTRAVI ET R+A +VNGVLRKTT DME+ GF+IPKGWRIYVYTREINY
Sbjct: 314  PIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREINY 373

Query: 1288 DQYLYPEPATFNPWRWLDKNLDSHPYFLLFGGGSRLCPGKELGIAEIATFLHYFVTRYRW 1467
            D  LYP+P  FNPWRWLDK+L+S  YFLLFGGG+R CPGKELGIAEI+TFLHYFVTRYRW
Sbjct: 374  DPLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRYRW 433

Query: 1468 EEVGGNHIVKFPRVEAPNGLRIRVFDY 1548
            EEVGG+ ++KFPRVEAPNGL IRV  Y
Sbjct: 434  EEVGGDKLMKFPRVEAPNGLHIRVSAY 460


>ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinifera]
            gi|298204461|emb|CBI16941.3| unnamed protein product
            [Vitis vinifera]
          Length = 464

 Score =  700 bits (1806), Expect = 0.0
 Identities = 336/447 (75%), Positives = 382/447 (85%)
 Frame = +1

Query: 208  SAMLRWNELRYRKKGLPPGTMGWPLFGETTEFLKQGPSFLKNQRARYGSLFKSHILGCPT 387
            +A+LRWNE+RYRKKGLPPGTMGWP+FGETTEFLKQGPSF+KNQRARYG  FKSH+LGCPT
Sbjct: 19   TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 78

Query: 388  VVSMDPELNRFILMNEGKGLVPGYPQSMLDILGKCNIAAVDGPLHKAMRGAMLGLINPPM 567
             VSMDPELNR+ILMNE KGLVPGYPQSMLDILGKCNIAAV G  HK MRGA+L LI+P M
Sbjct: 79   TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 138

Query: 568  IRDQLLHKIDQFMRSHLTDWSNKIIDIQQKTKEMALLSALKQIASIETGSFAESLKTEIF 747
            IR QLL KID+FMRSHL  W  KII+IQ+KTKEMALLS+LKQIA IE+G+ ++    E F
Sbjct: 139  IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEFMPEFF 198

Query: 748  KLVVGTLSLPINLPGTNYRRGLQARKKAVGMLRDLIQQRRESNCSQNDMLDCLLKNDGST 927
            KLV+GT+SLPI+LPGTNYRRG QARK  VGMLR LI++R+ S  + NDML CL++ +   
Sbjct: 199  KLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTN-EN 257

Query: 928  RSMLSDDQIIDLIIALVYSGYETVSTTSMMAVKYLLDHPKALEELRKEHFGIRNGKASEE 1107
            R  LSD++IIDLII ++YSGYETVSTTSMMAVKYL DHP+ L+ELRKEH  IR  K  E+
Sbjct: 258  RYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRPED 317

Query: 1108 GIDWNDYKSMRFTRAVILETLRVAAVVNGVLRKTTHDMEMKGFLIPKGWRIYVYTREINY 1287
             IDWNDYK MRFTRAVI ET R+A +VNGVLRKTT DME+ GF+IPKGWRIYVYTREINY
Sbjct: 318  PIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREINY 377

Query: 1288 DQYLYPEPATFNPWRWLDKNLDSHPYFLLFGGGSRLCPGKELGIAEIATFLHYFVTRYRW 1467
            D  LYP+P  FNPWRWLDK+L+S  YFLLFGGG+R CPGKELGIAEI+TFLHYFVTRYRW
Sbjct: 378  DPLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRYRW 437

Query: 1468 EEVGGNHIVKFPRVEAPNGLRIRVFDY 1548
            EEVGG+ ++KFPRVEAPNGL IRV  Y
Sbjct: 438  EEVGGDKLMKFPRVEAPNGLHIRVSAY 464


>ref|XP_003554965.1| PREDICTED: cytochrome P450 85A-like [Glycine max]
          Length = 465

 Score =  691 bits (1783), Expect = 0.0
 Identities = 330/447 (73%), Positives = 384/447 (85%)
 Frame = +1

Query: 208  SAMLRWNELRYRKKGLPPGTMGWPLFGETTEFLKQGPSFLKNQRARYGSLFKSHILGCPT 387
            SA+LRWNE+RYRKKGLPPGTMGWPLFGETTEFLKQGP+F+K QRARYGS FKSHILGCPT
Sbjct: 20   SALLRWNEVRYRKKGLPPGTMGWPLFGETTEFLKQGPNFMKTQRARYGSFFKSHILGCPT 79

Query: 388  VVSMDPELNRFILMNEGKGLVPGYPQSMLDILGKCNIAAVDGPLHKAMRGAMLGLINPPM 567
            +VSMDPELNR+ILMNE KGLVPGYPQSMLDILGKCNIAAV G  HK MRGA+L +I+P +
Sbjct: 80   IVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLSIISPTL 139

Query: 568  IRDQLLHKIDQFMRSHLTDWSNKIIDIQQKTKEMALLSALKQIASIETGSFAESLKTEIF 747
            IRDQLL KIDQFMR+HL++W +K+I+IQ+KTKEMA LS+LKQIA +E+GS ++S   E F
Sbjct: 140  IRDQLLPKIDQFMRAHLSNWGDKVINIQEKTKEMAFLSSLKQIAGMESGSLSDSFMAEFF 199

Query: 748  KLVVGTLSLPINLPGTNYRRGLQARKKAVGMLRDLIQQRRESNCSQNDMLDCLLKNDGST 927
            KLV+GTLSLPI+LPGTNY  G QARK  V +L  L+++RR S+ + +DML CL+  D S 
Sbjct: 200  KLVLGTLSLPIDLPGTNYHSGFQARKTIVNILSKLLEERRASHETYHDMLGCLMGRDES- 258

Query: 928  RSMLSDDQIIDLIIALVYSGYETVSTTSMMAVKYLLDHPKALEELRKEHFGIRNGKASEE 1107
            R  LSD++IIDL+I ++YSGYETVSTTSMMAVKYL DHPKALEELRKEH  IR  K  +E
Sbjct: 259  RYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKALEELRKEHLAIRERKKPDE 318

Query: 1108 GIDWNDYKSMRFTRAVILETLRVAAVVNGVLRKTTHDMEMKGFLIPKGWRIYVYTREINY 1287
             +D ND KSMRFTRAVI ET R+A +VNGVLRKTT DME+ G+LIPKGWRIYVYTREINY
Sbjct: 319  PLDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTQDMELNGYLIPKGWRIYVYTREINY 378

Query: 1288 DQYLYPEPATFNPWRWLDKNLDSHPYFLLFGGGSRLCPGKELGIAEIATFLHYFVTRYRW 1467
            D +LYP+P TFNPWRW+DK+L+S  YF +FGGG+R CPGKELGI EI+TFLHYFVTRYRW
Sbjct: 379  DPFLYPDPLTFNPWRWMDKSLESKNYFFIFGGGTRQCPGKELGITEISTFLHYFVTRYRW 438

Query: 1468 EEVGGNHIVKFPRVEAPNGLRIRVFDY 1548
            EEVGG+ ++KFPRVEAPNGL IRV  Y
Sbjct: 439  EEVGGDKVMKFPRVEAPNGLHIRVTSY 465


>ref|XP_003544008.1| PREDICTED: cytochrome P450 85A-like [Glycine max]
          Length = 464

 Score =  688 bits (1776), Expect = 0.0
 Identities = 328/447 (73%), Positives = 383/447 (85%)
 Frame = +1

Query: 208  SAMLRWNELRYRKKGLPPGTMGWPLFGETTEFLKQGPSFLKNQRARYGSLFKSHILGCPT 387
            SA+LRWNE+RYRKKGLPPGTMGWPLFGETTEFLKQGP+F+K QR+RYGS FKSHILGCPT
Sbjct: 19   SALLRWNEVRYRKKGLPPGTMGWPLFGETTEFLKQGPNFMKTQRSRYGSFFKSHILGCPT 78

Query: 388  VVSMDPELNRFILMNEGKGLVPGYPQSMLDILGKCNIAAVDGPLHKAMRGAMLGLINPPM 567
            +VSMDPELNR+ILMNE KGLVPGYPQSMLDILGKCNIAAV G  HK MRGA+L +I+P +
Sbjct: 79   IVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLSIISPTL 138

Query: 568  IRDQLLHKIDQFMRSHLTDWSNKIIDIQQKTKEMALLSALKQIASIETGSFAESLKTEIF 747
            IRDQLL KIDQFMR+HL++W +K+I+IQ+KTKEMA LS+LKQIA +E+GS ++SL  E F
Sbjct: 139  IRDQLLQKIDQFMRAHLSNWDDKVINIQEKTKEMAFLSSLKQIAGMESGSLSDSLMAEFF 198

Query: 748  KLVVGTLSLPINLPGTNYRRGLQARKKAVGMLRDLIQQRRESNCSQNDMLDCLLKNDGST 927
            KLV+GTLSLPINLP TNY  G QARK  V +L  L+++RR S+ + +DML CL+  D S 
Sbjct: 199  KLVLGTLSLPINLPSTNYHHGFQARKTIVKILSKLLEERRASHETYHDMLGCLMGRDES- 257

Query: 928  RSMLSDDQIIDLIIALVYSGYETVSTTSMMAVKYLLDHPKALEELRKEHFGIRNGKASEE 1107
            R  LSD++IIDL+I + YSGYETVSTTSMMAVKYL DHPKALEELRKEH  IR  K  +E
Sbjct: 258  RYKLSDEEIIDLVITITYSGYETVSTTSMMAVKYLHDHPKALEELRKEHLAIRERKKPDE 317

Query: 1108 GIDWNDYKSMRFTRAVILETLRVAAVVNGVLRKTTHDMEMKGFLIPKGWRIYVYTREINY 1287
             +D ND KSM+FTRAVI ET R+A +VNGVLRKTT DME+ G+LIPKGWRIYVYTREINY
Sbjct: 318  PLDCNDLKSMKFTRAVIFETSRLATIVNGVLRKTTQDMELNGYLIPKGWRIYVYTREINY 377

Query: 1288 DQYLYPEPATFNPWRWLDKNLDSHPYFLLFGGGSRLCPGKELGIAEIATFLHYFVTRYRW 1467
            D +LYP+P TFNPWRW+DK+L+S  YF +FGGG+R CPGKELGI EI+TFLHYFVTRYRW
Sbjct: 378  DPFLYPDPLTFNPWRWMDKSLESKNYFFIFGGGTRQCPGKELGITEISTFLHYFVTRYRW 437

Query: 1468 EEVGGNHIVKFPRVEAPNGLRIRVFDY 1548
            EEVGG+ +++FPRVEAPNGL IRV  Y
Sbjct: 438  EEVGGDKVMRFPRVEAPNGLHIRVRSY 464


>gb|ACR20476.1| steroid C-6 oxidase [Gossypium hirsutum]
          Length = 465

 Score =  687 bits (1773), Expect = 0.0
 Identities = 329/447 (73%), Positives = 379/447 (84%)
 Frame = +1

Query: 208  SAMLRWNELRYRKKGLPPGTMGWPLFGETTEFLKQGPSFLKNQRARYGSLFKSHILGCPT 387
            +A+LRWNE+RYRKKGLPPGTMGWP+FGETTEFLKQGP+F+KNQRARYGS FKSHILGCPT
Sbjct: 19   TAVLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPNFMKNQRARYGSFFKSHILGCPT 78

Query: 388  VVSMDPELNRFILMNEGKGLVPGYPQSMLDILGKCNIAAVDGPLHKAMRGAMLGLINPPM 567
            VVSMDPELNR+ILMNE KGLVPGYPQSMLDILGKCNIAAV G  HK MRGA+L LI+P M
Sbjct: 79   VVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKQMRGALLALISPTM 138

Query: 568  IRDQLLHKIDQFMRSHLTDWSNKIIDIQQKTKEMALLSALKQIASIETGSFAESLKTEIF 747
            IR QLL KID+FMR+ L++W N++IDIQ +TKEMA LS+LKQIAS E+ S A+    E F
Sbjct: 139  IRQQLLPKIDEFMRAFLSNWDNQVIDIQDRTKEMAFLSSLKQIASAESSSVAQQFMPEFF 198

Query: 748  KLVVGTLSLPINLPGTNYRRGLQARKKAVGMLRDLIQQRRESNCSQNDMLDCLLKNDGST 927
            KLV+GTLSL I+LPGTNYRRG QAR+  + +L  LI++RR+S  S  DML  L++ND + 
Sbjct: 199  KLVLGTLSLHIDLPGTNYRRGFQARQNILKILGQLIEKRRDSEESHKDMLGYLMRNDDNN 258

Query: 928  RSMLSDDQIIDLIIALVYSGYETVSTTSMMAVKYLLDHPKALEELRKEHFGIRNGKASEE 1107
            R  LSDD+IID II ++YSGYETVSTTSMMAVKYL DHP  LEELRKEH GIR  K ++E
Sbjct: 259  RHKLSDDEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLEELRKEHMGIRERKRADE 318

Query: 1108 GIDWNDYKSMRFTRAVILETLRVAAVVNGVLRKTTHDMEMKGFLIPKGWRIYVYTREINY 1287
             I+WND KSMRFTRAVI ET R+A +VNGVLRKTT DME+ G++IPKGWRIYVYTREINY
Sbjct: 319  PIEWNDLKSMRFTRAVIFETSRLATIVNGVLRKTTQDMELNGYVIPKGWRIYVYTREINY 378

Query: 1288 DQYLYPEPATFNPWRWLDKNLDSHPYFLLFGGGSRLCPGKELGIAEIATFLHYFVTRYRW 1467
            D +LYP+P  FNPWRW+ K L+S  YFL+FGGG+R CPGKELGIAEI+TFLHYFVTRYRW
Sbjct: 379  DPFLYPDPLAFNPWRWMVKGLESQNYFLIFGGGTRQCPGKELGIAEISTFLHYFVTRYRW 438

Query: 1468 EEVGGNHIVKFPRVEAPNGLRIRVFDY 1548
            EEVGG  ++KFPRVEAPNGL IRV  Y
Sbjct: 439  EEVGGEKLMKFPRVEAPNGLHIRVSSY 465