BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00004835
         (2300 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268002.1| PREDICTED: d-2-hydroxyglutarate dehydrogenas...   661   0.0  
ref|XP_002310828.1| predicted protein [Populus trichocarpa] gi|2...   659   0.0  
ref|XP_004146548.1| PREDICTED: d-2-hydroxyglutarate dehydrogenas...   644   0.0  
ref|NP_001141496.1| uncharacterized protein LOC100273608 [Zea ma...   632   0.0  
ref|XP_003561332.1| PREDICTED: probable D-2-hydroxyglutarate deh...   626   0.0  

>ref|XP_002268002.1| PREDICTED: d-2-hydroxyglutarate dehydrogenase, mitochondrial-like
            [Vitis vinifera]
          Length = 552

 Score =  661 bits (1706), Expect(2) = 0.0
 Identities = 320/417 (76%), Positives = 372/417 (89%)
 Frame = +3

Query: 372  RIHQRSFGSTTINIQRNPSFSMINSDDISYFRDILDDNTVIQDEDKLSSANIDWMQKYKG 551
            RI  R +GS T  IQRNP FS INSDDI++FR+IL +  VIQDED+LS+AN+DWM+KYKG
Sbjct: 64   RIQNRFYGSATTKIQRNPIFSTINSDDINHFREILGEKNVIQDEDRLSAANMDWMRKYKG 123

Query: 552  SSKLLLLPKDTDEVSKILRYCNSRRLAVVPQGGNTGLVGGSVPVYDEVIVNVGLMNKIIS 731
            SSKLLL P+ T+EVS+IL+YCNSR LAVVPQGGNTGLVGGSVPV+DEVI+N+G MN IIS
Sbjct: 124  SSKLLLQPRSTEEVSQILKYCNSRCLAVVPQGGNTGLVGGSVPVFDEVIINIGSMNNIIS 183

Query: 732  LDKVSGILKCEAGCILENLDSFLANECYLMPIDLGAKGSCQIGGNVSTNAGGLHFVRYGS 911
             DKVSGIL CEAGCILENL SF+ N+ ++MP+DLGAKGSCQIGGN+STNAGGL  VRYGS
Sbjct: 184  FDKVSGILVCEAGCILENLISFVDNQGFIMPLDLGAKGSCQIGGNISTNAGGLRLVRYGS 243

Query: 912  LHGNVLGLEVVLADGTVLDMLGSLRKDNTGYDLKHLFIGSEGTLGIVTKVSILTPARLSS 1091
            LHGNVLGLE VLA+GTVLDMLG+LRKDNTGYDLKHLFIGSEG+LG+VTKVSILTP +LSS
Sbjct: 244  LHGNVLGLEAVLANGTVLDMLGTLRKDNTGYDLKHLFIGSEGSLGVVTKVSILTPPKLSS 303

Query: 1092 TNLAFLACEDYSSCQELLLKAKRGLGEVLSAFEFLDTNSMDLVLDKLEGVRNPFPSSLHK 1271
             N+AFLAC+DY SCQ+LLL+AKR LGE+LSAFEF+D  S ++VL+ LEG RNP P S+H 
Sbjct: 304  VNVAFLACKDYLSCQKLLLEAKRKLGEILSAFEFIDNQSFNVVLNHLEGARNPLPPSMHN 363

Query: 1272 FYVLIETTGTDDSYDREKLETFLVNSMEGGLISDGVIAQDINQASSFWRIREGITEALMK 1451
            FYVLIETTG+D+SYD+EKLE FL++SMEGGL+SDGV+AQDINQASSFW IREGI E++MK
Sbjct: 364  FYVLIETTGSDESYDKEKLEAFLLHSMEGGLVSDGVLAQDINQASSFWHIREGIPESIMK 423

Query: 1452 AGAVYKYDLSLPVEKLYDIVVEMQLRLGSSAKVCGYGHLGDGNLHLNISTPNYDDNV 1622
            AGAVYKYDLS+PVEK+YD+V EM++RLG SAKV GYGHLGD NLHLNISTP YDD +
Sbjct: 424  AGAVYKYDLSIPVEKMYDLVEEMRVRLGHSAKVMGYGHLGDSNLHLNISTPQYDDAI 480



 Score =  122 bits (306), Expect(2) = 0.0
 Identities = 56/69 (81%), Positives = 65/69 (94%)
 Frame = +2

Query: 1661 VFAQIEPFVYQWTAAHRGSISAEHGLGLMKADKIHYSKSTETVRLMASIKKLVDPNGIMN 1840
            + AQIEPFVY+WT+ HRGSISAEHG+GLMKA+KIHYSKS ETV+LMASIKKL+DPNGI+N
Sbjct: 480  ILAQIEPFVYEWTSKHRGSISAEHGVGLMKANKIHYSKSAETVQLMASIKKLLDPNGILN 539

Query: 1841 PYKVLPQSI 1867
            PYKVLP S+
Sbjct: 540  PYKVLPGSL 548


>ref|XP_002310828.1| predicted protein [Populus trichocarpa] gi|222853731|gb|EEE91278.1|
            predicted protein [Populus trichocarpa]
          Length = 530

 Score =  659 bits (1701), Expect(2) = 0.0
 Identities = 321/413 (77%), Positives = 369/413 (89%)
 Frame = +3

Query: 384  RSFGSTTINIQRNPSFSMINSDDISYFRDILDDNTVIQDEDKLSSANIDWMQKYKGSSKL 563
            RSFGS    ++RNPSFS +NSDD+SYF+ +L +  V+QDED+L +ANIDWM KYKGSSKL
Sbjct: 49   RSFGSLATKVERNPSFSSLNSDDVSYFKGVLGEKNVVQDEDRLETANIDWMHKYKGSSKL 108

Query: 564  LLLPKDTDEVSKILRYCNSRRLAVVPQGGNTGLVGGSVPVYDEVIVNVGLMNKIISLDKV 743
            LLLP++T+EVSKIL YCNSRRLAVVPQGGNTGLVGGSVPV+DEVI+N G MNKII+ DKV
Sbjct: 109  LLLPRNTEEVSKILEYCNSRRLAVVPQGGNTGLVGGSVPVFDEVIINAGSMNKIIAFDKV 168

Query: 744  SGILKCEAGCILENLDSFLANECYLMPIDLGAKGSCQIGGNVSTNAGGLHFVRYGSLHGN 923
            SGIL CEAGCILENL S+L N+ ++MP+DLGAKGSCQIGGNVSTNAGGL FVRYGSLHGN
Sbjct: 169  SGILVCEAGCILENLISYLDNQGFIMPLDLGAKGSCQIGGNVSTNAGGLRFVRYGSLHGN 228

Query: 924  VLGLEVVLADGTVLDMLGSLRKDNTGYDLKHLFIGSEGTLGIVTKVSILTPARLSSTNLA 1103
            VLGLE VLA+G VLDMLG+LRKDNTGYDLKHLFIGSEG+LGIVTKVSILTP +LSS N+A
Sbjct: 229  VLGLEAVLANGDVLDMLGTLRKDNTGYDLKHLFIGSEGSLGIVTKVSILTPPKLSSVNIA 288

Query: 1104 FLACEDYSSCQELLLKAKRGLGEVLSAFEFLDTNSMDLVLDKLEGVRNPFPSSLHKFYVL 1283
            FLACEDY SCQ+LL +AKR LGE+LSAFEFLD+++MDLVL+ LEGVRNP PS++H FYVL
Sbjct: 289  FLACEDYLSCQKLLSEAKRKLGEILSAFEFLDSHAMDLVLNHLEGVRNPLPSAVHNFYVL 348

Query: 1284 IETTGTDDSYDREKLETFLVNSMEGGLISDGVIAQDINQASSFWRIREGITEALMKAGAV 1463
            IETTG+D+SYD+EKLE FL++SME GLISDGV+AQDINQASSFWRIREG+ EALM+AG V
Sbjct: 349  IETTGSDESYDKEKLEAFLLHSMESGLISDGVLAQDINQASSFWRIREGVPEALMRAGPV 408

Query: 1464 YKYDLSLPVEKLYDIVVEMQLRLGSSAKVCGYGHLGDGNLHLNISTPNYDDNV 1622
            YKYDLS+PVEK+Y +V EM+LRL   A V GYGHLGDGNLHLNIS P YDD +
Sbjct: 409  YKYDLSIPVEKMYSLVEEMRLRL---ANVVGYGHLGDGNLHLNISAPRYDDTI 458



 Score =  117 bits (293), Expect(2) = 0.0
 Identities = 54/69 (78%), Positives = 64/69 (92%)
 Frame = +2

Query: 1661 VFAQIEPFVYQWTAAHRGSISAEHGLGLMKADKIHYSKSTETVRLMASIKKLVDPNGIMN 1840
            + AQIEP+VY+WT+ HRGSISAEHGLGLMKA++I YSKS ETV+LMASIKKL+DPNGI+N
Sbjct: 458  ILAQIEPYVYEWTSKHRGSISAEHGLGLMKANEIFYSKSHETVQLMASIKKLLDPNGILN 517

Query: 1841 PYKVLPQSI 1867
            PYKVLP S+
Sbjct: 518  PYKVLPHSL 526


>ref|XP_004146548.1| PREDICTED: d-2-hydroxyglutarate dehydrogenase, mitochondrial-like
            [Cucumis sativus] gi|449500041|ref|XP_004160987.1|
            PREDICTED: d-2-hydroxyglutarate dehydrogenase,
            mitochondrial-like [Cucumis sativus]
          Length = 554

 Score =  644 bits (1661), Expect(2) = 0.0
 Identities = 315/413 (76%), Positives = 362/413 (87%)
 Frame = +3

Query: 384  RSFGSTTINIQRNPSFSMINSDDISYFRDILDDNTVIQDEDKLSSANIDWMQKYKGSSKL 563
            R   S +  +QRNPSFS +NSDDI +FR IL +  V+QDED+L  AN DW++KY+GSSKL
Sbjct: 72   RRIASLSSLVQRNPSFSRLNSDDIEFFRSILGEKNVVQDEDRLLDANTDWLRKYRGSSKL 131

Query: 564  LLLPKDTDEVSKILRYCNSRRLAVVPQGGNTGLVGGSVPVYDEVIVNVGLMNKIISLDKV 743
            LL P+ T+EVS+IL+YCNSR L VVPQGGNTGLVGGSVPV+DEVI+N+ LMN I+S DKV
Sbjct: 132  LLQPRSTEEVSQILKYCNSRDLPVVPQGGNTGLVGGSVPVFDEVIINLRLMNDIVSFDKV 191

Query: 744  SGILKCEAGCILENLDSFLANECYLMPIDLGAKGSCQIGGNVSTNAGGLHFVRYGSLHGN 923
            SGIL CEAG ILENL SFL N+ ++MP+DLGAKGSCQIGGNVSTNAGGL  VRYGSLHG+
Sbjct: 192  SGILVCEAGGILENLSSFLDNQGFIMPLDLGAKGSCQIGGNVSTNAGGLRLVRYGSLHGS 251

Query: 924  VLGLEVVLADGTVLDMLGSLRKDNTGYDLKHLFIGSEGTLGIVTKVSILTPARLSSTNLA 1103
            VLGLEVVLADG VLDMLG+LRKDNTGYDLKHLFIGSEGTLGI+TK+SILTP +L +TN+A
Sbjct: 252  VLGLEVVLADGRVLDMLGTLRKDNTGYDLKHLFIGSEGTLGIITKISILTPPKLPATNVA 311

Query: 1104 FLACEDYSSCQELLLKAKRGLGEVLSAFEFLDTNSMDLVLDKLEGVRNPFPSSLHKFYVL 1283
            FL C+DYSSCQ+LL+ AKR LGE+LSAFEFLD  SMDLVL+ LEG+RNP P ++H FYVL
Sbjct: 312  FLGCKDYSSCQKLLVDAKRKLGEILSAFEFLDNMSMDLVLNHLEGIRNPLPPTMHNFYVL 371

Query: 1284 IETTGTDDSYDREKLETFLVNSMEGGLISDGVIAQDINQASSFWRIREGITEALMKAGAV 1463
            IETTGTD+S D+EKLE FL+ SMEGGLISDG +AQDINQ SSFW+IREGI EALMKAGAV
Sbjct: 372  IETTGTDESSDKEKLEAFLLRSMEGGLISDGALAQDINQISSFWQIREGIPEALMKAGAV 431

Query: 1464 YKYDLSLPVEKLYDIVVEMQLRLGSSAKVCGYGHLGDGNLHLNISTPNYDDNV 1622
            YKYDLSLPVEK+YD+V EM++RLG+SAKV GYGHLGDGNLHLNISTP YDD V
Sbjct: 432  YKYDLSLPVEKMYDLVEEMRVRLGNSAKVIGYGHLGDGNLHLNISTPQYDDAV 484



 Score =  114 bits (284), Expect(2) = 0.0
 Identities = 53/69 (76%), Positives = 62/69 (89%)
 Frame = +2

Query: 1661 VFAQIEPFVYQWTAAHRGSISAEHGLGLMKADKIHYSKSTETVRLMASIKKLVDPNGIMN 1840
            VFAQIEPFVY+WT+ HRGSISAEHGLGLMKA+KI Y+KS E V++M SIKKL+DP GI+N
Sbjct: 484  VFAQIEPFVYEWTSNHRGSISAEHGLGLMKANKILYTKSPEIVQIMGSIKKLLDPRGILN 543

Query: 1841 PYKVLPQSI 1867
            PYKVLP S+
Sbjct: 544  PYKVLPHSL 552


>ref|NP_001141496.1| uncharacterized protein LOC100273608 [Zea mays]
            gi|194704812|gb|ACF86490.1| unknown [Zea mays]
          Length = 562

 Score =  632 bits (1630), Expect(2) = 0.0
 Identities = 305/456 (66%), Positives = 380/456 (83%), Gaps = 4/456 (0%)
 Frame = +3

Query: 267  LKSYEKFNPLRHV----CPMASRGGVLHRLNKESLIATSRIHQRSFGSTTINIQRNPSFS 434
            + SY +F+ +  V    C   S GG+     + S      + +R+FGS     QRNP++S
Sbjct: 40   VNSYRRFHWIPSVQHPLCGPTSSGGIYE--GQRSANKACEVQKRTFGSAATQFQRNPAYS 97

Query: 435  MINSDDISYFRDILDDNTVIQDEDKLSSANIDWMQKYKGSSKLLLLPKDTDEVSKILRYC 614
            ++NSDDI+YF+ IL +N V+QDED+++ AN+DWM KY+G+S+L+LLPK T EVSKIL YC
Sbjct: 98   VLNSDDIAYFKSILGENGVVQDEDRVAVANVDWMGKYRGASQLVLLPKTTTEVSKILSYC 157

Query: 615  NSRRLAVVPQGGNTGLVGGSVPVYDEVIVNVGLMNKIISLDKVSGILKCEAGCILENLDS 794
            N++RLAVVPQGGNTGLVGGSVPV+DEVIV +  MNKIIS D V+GIL CEAGC+LENL +
Sbjct: 158  NTKRLAVVPQGGNTGLVGGSVPVFDEVIVGLAGMNKIISFDNVNGILTCEAGCVLENLST 217

Query: 795  FLANECYLMPIDLGAKGSCQIGGNVSTNAGGLHFVRYGSLHGNVLGLEVVLADGTVLDML 974
            F+ NE ++MP+DLGAKGSC IGGN+STNAGGL F+RYGSLHGNVLGLEVVLADGT+LDML
Sbjct: 218  FVENEGFIMPLDLGAKGSCHIGGNISTNAGGLRFIRYGSLHGNVLGLEVVLADGTILDML 277

Query: 975  GSLRKDNTGYDLKHLFIGSEGTLGIVTKVSILTPARLSSTNLAFLACEDYSSCQELLLKA 1154
             +LRKDNTGYDLKHLFIGSEG+LG+VTK+S+LTPA+LSSTN+AFL+C DY+SCQ+LLL A
Sbjct: 278  TTLRKDNTGYDLKHLFIGSEGSLGVVTKISVLTPAKLSSTNVAFLSCNDYTSCQKLLLAA 337

Query: 1155 KRGLGEVLSAFEFLDTNSMDLVLDKLEGVRNPFPSSLHKFYVLIETTGTDDSYDREKLET 1334
            +R LGE+LSAFEF+D + +DL +  LEGV+NP P+S +KFYVL+ETTG+D+S D+ KLE 
Sbjct: 338  RRSLGEILSAFEFMDHHCIDLAMQHLEGVQNPLPASPYKFYVLVETTGSDESSDKTKLEA 397

Query: 1335 FLVNSMEGGLISDGVIAQDINQASSFWRIREGITEALMKAGAVYKYDLSLPVEKLYDIVV 1514
            FL+ SME GL++DGVIAQDI+QAS+FWRIREGI+EA +K GAVYKYDLS+ VEKLYDIV 
Sbjct: 398  FLLRSMEDGLVADGVIAQDISQASNFWRIREGISEASVKVGAVYKYDLSITVEKLYDIVE 457

Query: 1515 EMQLRLGSSAKVCGYGHLGDGNLHLNISTPNYDDNV 1622
            EM+ RLG +A+V GYGHLGDGNLHLNI +  YDDN+
Sbjct: 458  EMRCRLGDNAEVLGYGHLGDGNLHLNIVSSKYDDNI 493



 Score =  120 bits (301), Expect(2) = 0.0
 Identities = 56/70 (80%), Positives = 64/70 (91%)
 Frame = +2

Query: 1661 VFAQIEPFVYQWTAAHRGSISAEHGLGLMKADKIHYSKSTETVRLMASIKKLVDPNGIMN 1840
            + AQIEPFVY+WT+A RGSISAEHGLGLMKA+KIHYSKS E V+LMASIKKL+DP  I+N
Sbjct: 493  ILAQIEPFVYEWTSAQRGSISAEHGLGLMKAEKIHYSKSPEAVQLMASIKKLLDPKSILN 552

Query: 1841 PYKVLPQSIL 1870
            PYKVLPQS+L
Sbjct: 553  PYKVLPQSVL 562


>ref|XP_003561332.1| PREDICTED: probable D-2-hydroxyglutarate dehydrogenase,
            mitochondrial-like [Brachypodium distachyon]
          Length = 621

 Score =  626 bits (1615), Expect(2) = 0.0
 Identities = 301/432 (69%), Positives = 370/432 (85%)
 Frame = +3

Query: 327  GVLHRLNKESLIATSRIHQRSFGSTTINIQRNPSFSMINSDDISYFRDILDDNTVIQDED 506
            GV      +S+  +  + +R+FGS    IQRNP++S++N DD+SYF+ IL +N V+QD+D
Sbjct: 116  GVATNEAHQSVNDSFEVQRRTFGSAAEPIQRNPAYSVLNPDDVSYFKSILGNNGVVQDKD 175

Query: 507  KLSSANIDWMQKYKGSSKLLLLPKDTDEVSKILRYCNSRRLAVVPQGGNTGLVGGSVPVY 686
            +++ AN+DWM KYKG+S+LLLLPK T+EVSKIL YC+SRRLAVVPQGGNTGLVGGSVPV+
Sbjct: 176  RVAVANVDWMGKYKGASQLLLLPKSTNEVSKILAYCDSRRLAVVPQGGNTGLVGGSVPVH 235

Query: 687  DEVIVNVGLMNKIISLDKVSGILKCEAGCILENLDSFLANECYLMPIDLGAKGSCQIGGN 866
            DEVIVN+G M+KI+S D V+GIL CEAGC+LENL +F+ NE ++MP+DLGAKGSCQIGGN
Sbjct: 236  DEVIVNLGGMDKIVSFDNVNGILTCEAGCVLENLSTFVENEGFIMPLDLGAKGSCQIGGN 295

Query: 867  VSTNAGGLHFVRYGSLHGNVLGLEVVLADGTVLDMLGSLRKDNTGYDLKHLFIGSEGTLG 1046
            +STNAGGL F+RYGSLHGNVLGLEVVLA+GTVLDML +LRKDNTGYDLKHLF+GSEG+LG
Sbjct: 296  ISTNAGGLRFIRYGSLHGNVLGLEVVLANGTVLDMLTTLRKDNTGYDLKHLFVGSEGSLG 355

Query: 1047 IVTKVSILTPARLSSTNLAFLACEDYSSCQELLLKAKRGLGEVLSAFEFLDTNSMDLVLD 1226
            IVTKV+ILTPA+L STN+AFL+C DY SCQ+LLL A+R LGE++SAFEF+D   +DL + 
Sbjct: 356  IVTKVAILTPAKLPSTNVAFLSCNDYMSCQKLLLAARRSLGEIISAFEFMDRQCIDLAMT 415

Query: 1227 KLEGVRNPFPSSLHKFYVLIETTGTDDSYDREKLETFLVNSMEGGLISDGVIAQDINQAS 1406
             LEGV NP P+  +KFYVLIETTG+D+SYD+ KLE FL+ SME GL++DGVIAQDI+QAS
Sbjct: 416  HLEGVHNPLPNLPYKFYVLIETTGSDESYDKAKLEAFLLRSMEDGLVTDGVIAQDISQAS 475

Query: 1407 SFWRIREGITEALMKAGAVYKYDLSLPVEKLYDIVVEMQLRLGSSAKVCGYGHLGDGNLH 1586
            +FWRIREGI+EA +K GAVYKYDLS+PVEKLYDIV EM+ R+G  A+V GYGHLGDGNLH
Sbjct: 476  NFWRIREGISEASVKVGAVYKYDLSIPVEKLYDIVEEMRSRVGDMAEVLGYGHLGDGNLH 535

Query: 1587 LNISTPNYDDNV 1622
            LNI +  Y DN+
Sbjct: 536  LNILSSKYSDNI 547



 Score =  123 bits (308), Expect(2) = 0.0
 Identities = 56/69 (81%), Positives = 64/69 (92%)
 Frame = +2

Query: 1661 VFAQIEPFVYQWTAAHRGSISAEHGLGLMKADKIHYSKSTETVRLMASIKKLVDPNGIMN 1840
            + AQIEPFVY+WTA HRGSISAEHGLGLMKA+KIHYSKS E V+LMASIKKL+DPN I+N
Sbjct: 547  ILAQIEPFVYEWTAGHRGSISAEHGLGLMKAEKIHYSKSPEAVQLMASIKKLIDPNSILN 606

Query: 1841 PYKVLPQSI 1867
            PYKVLP+S+
Sbjct: 607  PYKVLPRSV 615