BLASTX nr result
ID: Dioscorea21_contig00003232
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00003232
(1770 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 cataly... 676 0.0
ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [R... 671 0.0
ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|2... 670 0.0
ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 cataly... 668 0.0
ref|XP_002315531.1| predicted protein [Populus trichocarpa] gi|2... 662 0.0
>ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Vitis
vinifera] gi|297736997|emb|CBI26198.3| unnamed protein
product [Vitis vinifera]
Length = 449
Score = 676 bits (1745), Expect = 0.0
Identities = 325/449 (72%), Positives = 370/449 (82%)
Frame = +2
Query: 62 MAEAVAPEMRWRDVNKLLARPGNIVAPTFEHSPQLRDDLGKYXXXXXXXXXXXXXXXXKD 241
MA++ A R RD++KLL RPG++V PTFE P+LRDDL K+ KD
Sbjct: 1 MADSTAHVSRSRDLDKLLLRPGHLVGPTFEPGPELRDDLRKFATVLVVGAGGLGCELLKD 60
Query: 242 LALMGFQNIDVIDMDTIEVSNLNRQFLFRLQDVGKSKAEVAAKRVMQRVSGVTIVPHFCR 421
LAL GF+N++VIDMD IEVSNLNRQFLFRLQDVGK KAEVAAKRVM+RVSGV IVPHFCR
Sbjct: 61 LALSGFKNLEVIDMDRIEVSNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120
Query: 422 IEDKDIEFYNNFQIIVLGLDSIEARSYINSVACSFLEYDDNDNPLEETVKPMVDGGTEGF 601
IEDKDIEFYN+F II LGLDSIEARSYIN+VACSFLEYD +D PLEET+KPMVDGGTEGF
Sbjct: 121 IEDKDIEFYNHFNIIALGLDSIEARSYINAVACSFLEYDSDDKPLEETIKPMVDGGTEGF 180
Query: 602 KGHARVIMPGTTPCFECNIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIRWDEVHSGKA 781
KGHARVI+PG TPCFEC IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLI+WDEVHSGKA
Sbjct: 181 KGHARVIIPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
Query: 782 FDADNPEHMQWIYSEAVKRADLFGITGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEAF 961
FD D+PEHM+W+YSEAVKRA+LFGI GVTYSLTQGVVK CALE
Sbjct: 241 FDPDDPEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
Query: 962 KLISGCSKTLSNYLTYNGLDGIHIKATDFVREKDCLVCGPGTLIELETSVTLQQFIESLA 1141
K++SGCSKTLSNYLTYNG +G+H K T+FV++ DCL+CGPG LIEL+TSVTLQ+F++ L
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQ 360
Query: 1142 EHPKLLISKPSVTYRGNNLYMQAPPILEEMTRANLNLPLFDLMDKVPKGTVHVSGTTNKE 1321
EHPKLL+SK SV +R NLY+QAPP+LEE+TR+NLNLPLFDLM K+ K VHV+G+T K
Sbjct: 361 EHPKLLLSKASVKHREKNLYIQAPPVLEELTRSNLNLPLFDLMGKIRKDIVHVTGSTTKN 420
Query: 1322 GKKTSCLRKLRVAFKGVDGIVDMDVAGAS 1408
KKTSCLRKLRV FKG+DG DMD AG +
Sbjct: 421 DKKTSCLRKLRVVFKGIDGATDMDTAGGA 449
>ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [Ricinus communis]
gi|223543819|gb|EEF45347.1| ubiquitin-activating enzyme
E1c, putative [Ricinus communis]
Length = 449
Score = 671 bits (1731), Expect = 0.0
Identities = 321/449 (71%), Positives = 372/449 (82%)
Frame = +2
Query: 62 MAEAVAPEMRWRDVNKLLARPGNIVAPTFEHSPQLRDDLGKYXXXXXXXXXXXXXXXXKD 241
MA++ R RD++KLL RPGN+VAPTF+ QLRDDL +Y KD
Sbjct: 1 MADSTVQPSRSRDLDKLLLRPGNLVAPTFDPGVQLRDDLQEYVRILVVGAGGLGCELLKD 60
Query: 242 LALMGFQNIDVIDMDTIEVSNLNRQFLFRLQDVGKSKAEVAAKRVMQRVSGVTIVPHFCR 421
LAL GF+N++VIDMD IEVSNLNRQFLFRL+DVGK KAEVAAKRVM+RVSGV IVPHFCR
Sbjct: 61 LALSGFKNLEVIDMDRIEVSNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120
Query: 422 IEDKDIEFYNNFQIIVLGLDSIEARSYINSVACSFLEYDDNDNPLEETVKPMVDGGTEGF 601
IEDK+++FYN+F IIVLGLDSIEARSYIN+VACSFLEYD DNP ++T+KPMVDGGTEGF
Sbjct: 121 IEDKELDFYNDFSIIVLGLDSIEARSYINNVACSFLEYDSEDNPRDDTMKPMVDGGTEGF 180
Query: 602 KGHARVIMPGTTPCFECNIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIRWDEVHSGKA 781
KGHARVIMPG TPCFEC IWLFPPQVKFPLCTLAETPRT AHCIEYAHLI+W+EVHSGKA
Sbjct: 181 KGHARVIMPGITPCFECTIWLFPPQVKFPLCTLAETPRTPAHCIEYAHLIKWNEVHSGKA 240
Query: 782 FDADNPEHMQWIYSEAVKRADLFGITGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEAF 961
FD D+PEHM+W+Y+EAVKRA+LFGI GVTYSLTQGVVK CALE
Sbjct: 241 FDPDDPEHMKWVYTEAVKRAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
Query: 962 KLISGCSKTLSNYLTYNGLDGIHIKATDFVREKDCLVCGPGTLIELETSVTLQQFIESLA 1141
K+ SGCSKTLSNYLTYNG++G+HIK T+FV++KDCL CGPG LIEL+TSV LQ+FI+ L
Sbjct: 301 KIASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLACGPGVLIELDTSVALQKFIDLLE 360
Query: 1142 EHPKLLISKPSVTYRGNNLYMQAPPILEEMTRANLNLPLFDLMDKVPKGTVHVSGTTNKE 1321
EHPKL +S+ SVT+RG NLYMQAPP+LEEMTR+NL+LPLF+LM KVPK VHV+G T +
Sbjct: 361 EHPKLFLSRASVTHRGKNLYMQAPPVLEEMTRSNLSLPLFELMGKVPKDIVHVTGMTGQN 420
Query: 1322 GKKTSCLRKLRVAFKGVDGIVDMDVAGAS 1408
GKKTSC+RKLRV FKG++GI DMD+AG +
Sbjct: 421 GKKTSCVRKLRVVFKGMEGITDMDMAGGA 449
>ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|222851659|gb|EEE89206.1|
predicted protein [Populus trichocarpa]
Length = 449
Score = 670 bits (1729), Expect = 0.0
Identities = 321/446 (71%), Positives = 370/446 (82%)
Frame = +2
Query: 62 MAEAVAPEMRWRDVNKLLARPGNIVAPTFEHSPQLRDDLGKYXXXXXXXXXXXXXXXXKD 241
MAE+ + R RD++KLL RPGN+VAPTFE QLRDDL +Y KD
Sbjct: 1 MAESTVQQSRSRDLDKLLLRPGNLVAPTFEPGAQLRDDLQEYARILVIGAGGLGCELLKD 60
Query: 242 LALMGFQNIDVIDMDTIEVSNLNRQFLFRLQDVGKSKAEVAAKRVMQRVSGVTIVPHFCR 421
LAL GF+N++VIDMD IEV+NLNRQFLFRL+DVGK KAEVAAKRVM+RVSGV IVPHFCR
Sbjct: 61 LALSGFKNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120
Query: 422 IEDKDIEFYNNFQIIVLGLDSIEARSYINSVACSFLEYDDNDNPLEETVKPMVDGGTEGF 601
IEDK+I+FY +F IIVLGLDS+EARSYIN+VAC FLEYD +DNP+EETVKPMVDGGTEGF
Sbjct: 121 IEDKEIDFYKDFIIIVLGLDSVEARSYINAVACGFLEYDSDDNPIEETVKPMVDGGTEGF 180
Query: 602 KGHARVIMPGTTPCFECNIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIRWDEVHSGKA 781
KGHARVI+PG+TPCFEC IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLI+WDEVHSGK
Sbjct: 181 KGHARVIIPGSTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240
Query: 782 FDADNPEHMQWIYSEAVKRADLFGITGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEAF 961
FD D+PEHM+W+Y+EAVKRA+LFGI GVTYSLTQGVVK C LE
Sbjct: 241 FDPDDPEHMKWVYTEAVKRAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300
Query: 962 KLISGCSKTLSNYLTYNGLDGIHIKATDFVREKDCLVCGPGTLIELETSVTLQQFIESLA 1141
KL SGCSKTLSNYLTYNG++G+HIK T+FV++KDCLVCGPG L+EL+TSVTLQ+FI+ L
Sbjct: 301 KLASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLE 360
Query: 1142 EHPKLLISKPSVTYRGNNLYMQAPPILEEMTRANLNLPLFDLMDKVPKGTVHVSGTTNKE 1321
EHPKLL+SK SV +R NLYMQAPP+LEEMTR+NLNLPLF+LM K PK VHV+GTT+K+
Sbjct: 361 EHPKLLLSKASVRHRATNLYMQAPPVLEEMTRSNLNLPLFELMGKFPKDIVHVTGTTSKD 420
Query: 1322 GKKTSCLRKLRVAFKGVDGIVDMDVA 1399
KKTSCLRKL + FKG D + D+D+A
Sbjct: 421 DKKTSCLRKLCLVFKGADAVTDLDMA 446
>ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit-like [Cucumis
sativus] gi|449515377|ref|XP_004164726.1| PREDICTED:
NEDD8-activating enzyme E1 catalytic subunit-like
[Cucumis sativus]
Length = 449
Score = 668 bits (1724), Expect = 0.0
Identities = 321/449 (71%), Positives = 368/449 (81%)
Frame = +2
Query: 62 MAEAVAPEMRWRDVNKLLARPGNIVAPTFEHSPQLRDDLGKYXXXXXXXXXXXXXXXXKD 241
MA +V R RD++KLL RPGN++ FE QLRDDL +Y KD
Sbjct: 1 MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
Query: 242 LALMGFQNIDVIDMDTIEVSNLNRQFLFRLQDVGKSKAEVAAKRVMQRVSGVTIVPHFCR 421
LAL GF+N++VIDMD IEV+NLNRQFLFRL+DVGK KAEVAAKRVM+RVSGV I+PHFCR
Sbjct: 61 LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
Query: 422 IEDKDIEFYNNFQIIVLGLDSIEARSYINSVACSFLEYDDNDNPLEETVKPMVDGGTEGF 601
IEDK+IEFYN+F II LGLDSIEARSYINSVACSFLEYD +DNPLEET+KPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
Query: 602 KGHARVIMPGTTPCFECNIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIRWDEVHSGKA 781
KGHARVI+PG TPCFEC IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLI+WDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240
Query: 782 FDADNPEHMQWIYSEAVKRADLFGITGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEAF 961
FD D+ EHM+W+YSEA+KRA+LFGI GVTYSLTQGVVK CALE
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
Query: 962 KLISGCSKTLSNYLTYNGLDGIHIKATDFVREKDCLVCGPGTLIELETSVTLQQFIESLA 1141
K++SGCSKTLSNYLTYNG +G+H K T+FV++KDCLVCGPG LIEL++S+TLQ+FI+ L
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
Query: 1142 EHPKLLISKPSVTYRGNNLYMQAPPILEEMTRANLNLPLFDLMDKVPKGTVHVSGTTNKE 1321
+HPKLL+SK SVT+RG NLYMQAPP+LEEMTR+NL +PLFDLM KV K VHV+G +K
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
Query: 1322 GKKTSCLRKLRVAFKGVDGIVDMDVAGAS 1408
KKTSCLRKLRVAFKGVDG+ DMD A +
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449
>ref|XP_002315531.1| predicted protein [Populus trichocarpa] gi|222864571|gb|EEF01702.1|
predicted protein [Populus trichocarpa]
Length = 449
Score = 662 bits (1709), Expect = 0.0
Identities = 321/446 (71%), Positives = 368/446 (82%)
Frame = +2
Query: 62 MAEAVAPEMRWRDVNKLLARPGNIVAPTFEHSPQLRDDLGKYXXXXXXXXXXXXXXXXKD 241
MA++ + R RD++KLL RPGN+VAPTFE QLRDDL +Y KD
Sbjct: 1 MADSTVQQSRSRDLDKLLLRPGNLVAPTFESGVQLRDDLQEYARLLVIGAGGLGCELLKD 60
Query: 242 LALMGFQNIDVIDMDTIEVSNLNRQFLFRLQDVGKSKAEVAAKRVMQRVSGVTIVPHFCR 421
LAL GF+N++VIDMD IEV+NLNRQFLFRL+DVGK KAEVAAKRVM+RVSGV IVPHFCR
Sbjct: 61 LALSGFKNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120
Query: 422 IEDKDIEFYNNFQIIVLGLDSIEARSYINSVACSFLEYDDNDNPLEETVKPMVDGGTEGF 601
IEDK+I+FY +F IIVLGLDS+EARSYIN+VAC FLEYD +DNPLEETVKPMVDGGTEGF
Sbjct: 121 IEDKEIDFYKDFSIIVLGLDSVEARSYINAVACGFLEYDSDDNPLEETVKPMVDGGTEGF 180
Query: 602 KGHARVIMPGTTPCFECNIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIRWDEVHSGKA 781
KGHARVIMPG+TPCFEC IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLI+WDEVHSGK
Sbjct: 181 KGHARVIMPGSTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240
Query: 782 FDADNPEHMQWIYSEAVKRADLFGITGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEAF 961
FD D+PEHM+W+Y+EAVKRA+LFGI GVTYSLTQGVVK C LE
Sbjct: 241 FDPDDPEHMKWVYTEAVKRAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300
Query: 962 KLISGCSKTLSNYLTYNGLDGIHIKATDFVREKDCLVCGPGTLIELETSVTLQQFIESLA 1141
KL SGCSKTLSNYLTYNG++G+HIK T+FVR+KDCLVCGPG LIEL+TSVTLQ+FI+ L
Sbjct: 301 KLASGCSKTLSNYLTYNGVEGLHIKVTEFVRDKDCLVCGPGVLIELDTSVTLQKFIDMLE 360
Query: 1142 EHPKLLISKPSVTYRGNNLYMQAPPILEEMTRANLNLPLFDLMDKVPKGTVHVSGTTNKE 1321
EHPKLL+SK SV ++ NLYMQAPP+LEEMTR+NL LPLF+LM KV K V V+GT++K+
Sbjct: 361 EHPKLLLSKASVRHQTMNLYMQAPPVLEEMTRSNLGLPLFELMGKVSKDIVLVTGTSSKD 420
Query: 1322 GKKTSCLRKLRVAFKGVDGIVDMDVA 1399
KKTSCLRKL + FKG D + DMD+A
Sbjct: 421 DKKTSCLRKLCLVFKGQDAVTDMDMA 446