BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00002481
         (1140 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   608   e-172
ref|XP_002307125.1| predicted protein [Populus trichocarpa] gi|2...   604   e-170
ref|NP_001048203.1| Os02g0762300 [Oryza sativa Japonica Group] g...   603   e-170
ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabid...   603   e-170
ref|XP_003570581.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   602   e-170

>ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 1
            [Vitis vinifera]
          Length = 356

 Score =  608 bits (1568), Expect = e-172
 Identities = 295/349 (84%), Positives = 317/349 (90%), Gaps = 3/349 (0%)
 Frame = -2

Query: 1046 KSRRGAGRVCSICNERKAALKRPKTLEQICRECFYTVFEDEIHRTIVDNHLFKPGERVAI 867
            K ++     CSIC++R+AALKRPKTLEQICRECFY VFE+EIHR +VDN LFKPGER+AI
Sbjct: 7    KPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKPGERIAI 66

Query: 866  GASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLK 687
            GASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLK
Sbjct: 67   GASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLK 126

Query: 686  IVSYKTLYGWTMDEIVKAIGLKNNCTFCGVFRRQALDRGAAVMKAEKLVTGHNADDMAET 507
            +VSYK LYGWTMDEIVK IGLKNNCTFCGVFRRQALDRGAA++K +KL TGHNADD+AET
Sbjct: 127  VVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAAMLKVDKLATGHNADDIAET 186

Query: 506  ILLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYS 327
            +LLNILRGDIARLSRCT I TGEDGPIPRCKPFKYTYEKEIVMYAYFK+LDYFSTECIYS
Sbjct: 187  VLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKYTYEKEIVMYAYFKRLDYFSTECIYS 246

Query: 326  PNAYRGFAREFIKDLERIRPRAILDIIRSGENFRISSSTKMPEQGTCERCGYISSQKLCK 147
            PNAYRGFAREFIKDLERIRPRAILDIIRSGE FRIS+S KMPEQGTCERCGYISSQK CK
Sbjct: 247  PNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRISTSAKMPEQGTCERCGYISSQKWCK 306

Query: 146  ACVLLDGLNRGLPKQGIGRSRGRNNAKD---EEHNKDSSFQSKPCGALD 9
            ACVLL+GLNRGLPK GIGR+RG NN  +   ++ N   + QSK CG LD
Sbjct: 307  ACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSNGVKNIQSKQCGTLD 355


>ref|XP_002307125.1| predicted protein [Populus trichocarpa] gi|222856574|gb|EEE94121.1|
            predicted protein [Populus trichocarpa]
          Length = 354

 Score =  604 bits (1558), Expect = e-170
 Identities = 294/348 (84%), Positives = 323/348 (92%), Gaps = 4/348 (1%)
 Frame = -2

Query: 1040 RRGAGRVCSICNERKAALKRPKTLEQICRECFYTVFEDEIHRTIVDNHLFKPGERVAIGA 861
            ++ AGR+C +CN+R+AALKRPKTLEQICRECFY VFE+EIH+ IV N LFKPGER+AIGA
Sbjct: 6    KKAAGRLCYLCNQRRAALKRPKTLEQICRECFYEVFEEEIHQVIVKNQLFKPGERIAIGA 65

Query: 860  SGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIV 681
            SGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIV
Sbjct: 66   SGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIV 125

Query: 680  SYKTLYGWTMDEIVKAIGLKNNCTFCGVFRRQALDRGAAVMKAEKLVTGHNADDMAETIL 501
            SYK LYGWTMDEIVK IGLKNNCTFCGVFRRQALDRGAA++K +KLVTGHNADD+AET+L
Sbjct: 126  SYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDIAETVL 185

Query: 500  LNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVM-YAYFKKLDYFSTECIYSP 324
            LNILRGDIARLSRCT ITTGEDGPIPRCKPFKYTYEKEI++ YAYFK+LDYFSTECIYSP
Sbjct: 186  LNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIMLTYAYFKRLDYFSTECIYSP 245

Query: 323  NAYRGFAREFIKDLERIRPRAILDIIRSGENFRISSSTKMPEQGTCERCGYISSQKLCKA 144
            NAYRGFAREFIKDLER+RPRAILDII+SGE+FRIS+STKMPEQGTCERCGYISSQK CKA
Sbjct: 246  NAYRGFAREFIKDLERMRPRAILDIIKSGEDFRISTSTKMPEQGTCERCGYISSQKWCKA 305

Query: 143  CVLLDGLNRGLPKQGIGRSRG--RNNAKD-EEHNKDSSFQSKPCGALD 9
            CVLL+GLN+GLPK GIGRSRG   N+ KD ++ N   + + K CG+LD
Sbjct: 306  CVLLEGLNKGLPKLGIGRSRGLDNNHRKDVKQSNGTKNIEGKQCGSLD 353


>ref|NP_001048203.1| Os02g0762300 [Oryza sativa Japonica Group]
            gi|75225396|sp|Q6Z6G6.1|CTU1_ORYSJ RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 1; AltName:
            Full=Cytoplasmic tRNA adenylyltransferase 1
            gi|46805899|dbj|BAD17212.1| n-type ATP
            pyrophosphatase-like [Oryza sativa Japonica Group]
            gi|113537734|dbj|BAF10117.1| Os02g0762300 [Oryza sativa
            Japonica Group] gi|215741300|dbj|BAG97795.1| unnamed
            protein product [Oryza sativa Japonica Group]
          Length = 363

 Score =  603 bits (1554), Expect = e-170
 Identities = 296/345 (85%), Positives = 316/345 (91%), Gaps = 8/345 (2%)
 Frame = -2

Query: 1037 RGAGRVCSICNERKAALKRPKTLEQICRECFYTVFEDEIHRTIVDNHLFKPGERVAIGAS 858
            R   R+C+ C ERKAALKRPKTLEQICRECFY VFEDEIH+TIVDN+LFKPG+RVAIGAS
Sbjct: 14   RAGSRLCTRCGERKAALKRPKTLEQICRECFYVVFEDEIHQTIVDNNLFKPGDRVAIGAS 73

Query: 857  GGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIVS 678
            GGKDSTVLAYVLSELNRRH Y LDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIVS
Sbjct: 74   GGKDSTVLAYVLSELNRRHKYCLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIVS 133

Query: 677  YKTLYGWTMDEIVKAIGLKNNCTFCGVFRRQALDRGAAVMKAEKLVTGHNADDMAETILL 498
            YK LYGWTMD+IVKAIGLKNNCTFCGVFRRQALDRGAA++K +K+VTGHNADD+AET+LL
Sbjct: 134  YKDLYGWTMDDIVKAIGLKNNCTFCGVFRRQALDRGAALLKVDKIVTGHNADDIAETVLL 193

Query: 497  NILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNA 318
            NILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNA
Sbjct: 194  NILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNA 253

Query: 317  YRGFAREFIKDLERIRPRAILDIIRSGENFRISSSTKMPEQGTCERCGYISSQKLCKACV 138
            YRGFAREFIKDLER+RPRAILDII+SGENFRIS++T+MPEQGTCERCGYISSQKLCKACV
Sbjct: 254  YRGFAREFIKDLERMRPRAILDIIKSGENFRISTTTRMPEQGTCERCGYISSQKLCKACV 313

Query: 137  LLDGLNRGLPKQGIGRSRG--------RNNAKDEEHNKDSSFQSK 27
            LLDGLNRGLPK GIGR++G           A   E N+ SS Q K
Sbjct: 314  LLDGLNRGLPKLGIGRTKGIAGGDGDCEQQATRSERNR-SSLQGK 357


>ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis thaliana]
            gi|226711456|sp|O64862.2|CTU1_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 1; AltName:
            Full=Cytoplasmic tRNA adenylyltransferase 1
            gi|26449617|dbj|BAC41934.1| unknown protein [Arabidopsis
            thaliana] gi|30725380|gb|AAP37712.1| At2g44270
            [Arabidopsis thaliana] gi|330255305|gb|AEC10399.1|
            cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis
            thaliana]
          Length = 355

 Score =  603 bits (1554), Expect = e-170
 Identities = 293/353 (83%), Positives = 317/353 (89%), Gaps = 4/353 (1%)
 Frame = -2

Query: 1055 DGKKSRRGAGRVCSICNERKAALKRPKTLEQICRECFYTVFEDEIHRTIVDNHLFKPGER 876
            + K  +  A R+C +CN R+  LKRPKTL+QICRECFY VFE+EIH+ IV N LFK GER
Sbjct: 2    EAKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGER 61

Query: 875  VAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGL 696
            VAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLS+DEGITGYRDDSLETVKRNE+QYGL
Sbjct: 62   VAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGL 121

Query: 695  PLKIVSYKTLYGWTMDEIVKAIGLKNNCTFCGVFRRQALDRGAAVMKAEKLVTGHNADDM 516
            PLKIVSYK LYGWTMDEIVK IGLKNNCTFCGVFRRQALDRGAA++K EKLVTGHNADD+
Sbjct: 122  PLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDI 181

Query: 515  AETILLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 336
            AET+LLNILRGDIARLSRCT ITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC
Sbjct: 182  AETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTEC 241

Query: 335  IYSPNAYRGFAREFIKDLERIRPRAILDIIRSGENFRISSSTKMPEQGTCERCGYISSQK 156
            IYSPNAYRGFAREFIKDLERIRPRAILDII+SGE+FRI+++TKMPEQGTCERCGYISSQK
Sbjct: 242  IYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQK 301

Query: 155  LCKACVLLDGLNRGLPKQGIGRSRGRNNAKDEEHNKD----SSFQSKPCGALD 9
             CKACVLL+GLNRGLPK GIGR RG N   ++E  K      S +SK CG+LD
Sbjct: 302  WCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETKKPGSVAKSIESKQCGSLD 354


>ref|XP_003570581.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like [Brachypodium
            distachyon]
          Length = 358

 Score =  602 bits (1553), Expect = e-170
 Identities = 298/348 (85%), Positives = 313/348 (89%), Gaps = 9/348 (2%)
 Frame = -2

Query: 1043 SRRGAGRVCSICNERKAALKRPKTLEQICRECFYTVFEDEIHRTIVDNHLFKPGERVAIG 864
            S R   R+C  C ERKAALKRPKTLEQICRECFY VFEDEIHRTIVDN LFKPGERVAIG
Sbjct: 6    STRAGSRLCVRCGERKAALKRPKTLEQICRECFYVVFEDEIHRTIVDNCLFKPGERVAIG 65

Query: 863  ASGGKDSTVLAYVLSELNRRHNYGLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKI 684
            ASGGKDSTVLAYVLSELN RH YGLDLFLLS+DEGITGYRDDSLETVKRNEIQYGLPLKI
Sbjct: 66   ASGGKDSTVLAYVLSELNNRHKYGLDLFLLSIDEGITGYRDDSLETVKRNEIQYGLPLKI 125

Query: 683  VSYKTLYGWTMDEIVKAIGLKNNCTFCGVFRRQALDRGAAVMKAEKLVTGHNADDMAETI 504
            VSYK LY WTMD+IVKAIGLKNNCTFCGVFRRQALDRGAA++K +K+VTGHNADD+AET+
Sbjct: 126  VSYKDLYDWTMDDIVKAIGLKNNCTFCGVFRRQALDRGAALLKVDKIVTGHNADDIAETV 185

Query: 503  LLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSP 324
            LLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSP
Sbjct: 186  LLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSP 245

Query: 323  NAYRGFAREFIKDLERIRPRAILDIIRSGENFRISSSTKMPEQGTCERCGYISSQKLCKA 144
            NAYRGFAREFIKDLER+RPRAILDII SGENFRIS++TKMPEQGTCERCGYISSQKLCKA
Sbjct: 246  NAYRGFAREFIKDLERMRPRAILDIITSGENFRISTTTKMPEQGTCERCGYISSQKLCKA 305

Query: 143  CVLLDGLNRGLPKQGIGRS---------RGRNNAKDEEHNKDSSFQSK 27
            CVLLDGLNRGLPK GIGR+          G    K +E N+ SS Q K
Sbjct: 306  CVLLDGLNRGLPKLGIGRTTKGGAGADGNGERPGKQKERNR-SSLQGK 352