BLASTX nr result
ID: Dioscorea21_contig00002066
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00002066
(4297 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAX19872.1| unknown, partial [Doryanthes excelsa] 89 8e-15
ref|XP_004079613.1| PREDICTED: uncharacterized protein LOC101155... 88 2e-14
ref|XP_002490879.1| Mucin-like protein [Komagataella pastoris GS... 81 2e-12
emb|CCA37660.1| Cell surface glycoprotein 1 [Komagataella pastor... 75 2e-10
ref|XP_002259727.1| hypothetical protein, conserved in Plasmodiu... 73 6e-10
>gb|AAX19872.1| unknown, partial [Doryanthes excelsa]
Length = 252
Score = 89.4 bits (220), Expect = 8e-15
Identities = 73/248 (29%), Positives = 122/248 (49%), Gaps = 19/248 (7%)
Frame = +3
Query: 2838 IKVAEKEAIAEKGSTSANDTEDKDSPVSSSFTQATQVALPRDANEVVEDNAKRSHGVEES 3017
+ + E + EK S+SA TEDKD ++ V L + N+ ED + ++ +
Sbjct: 5 LNLVSNEQVNEKESSSAELTEDKD------YSHKLSVVLEPEVNQPAEDTDR----LQLA 54
Query: 3018 DNSQHEESLQSDPADLPVSRFLMDHIL-HQGDVPDEVGNDESKNNIEEETTSDKLMTISI 3194
+ + HEE Q+D DLPVSRFLMDHIL +GD ++ + ESK+ I EE + + I
Sbjct: 55 EGASHEEKQQTDLHDLPVSRFLMDHILREEGDSLNKACDLESKDKIHEENDGGEQNEVEI 114
Query: 3195 QEQEIFMDRLSAELVPSENIV--ENKGMEKLEDTI-LQQDNMPADVCD-----VEADNEI 3350
+QE + LSAE V E + ++ G +KLE++ L Q+ DV + V D+
Sbjct: 115 PKQEGILVSLSAEQVTEETSISEDSVGHKKLENSSHLVQEQQSHDVKENTEIPVVKDDVT 174
Query: 3351 KQKHTTDEQ----MGTDIQEKE-AIMDSVSAQQMPEEKSTSEDRDQEKPG-----DHFLE 3500
Q D +G DI+++ ++ VS +P + +Q PG + ++
Sbjct: 175 PQSSLHDSSPTVAVGEDIKDEVIRLLSEVSTHVVPNDSMELTSTEQNTPGIKVDTEECMD 234
Query: 3501 EGIAPEDV 3524
G+ +++
Sbjct: 235 PGLVQDEI 242
Score = 59.3 bits (142), Expect = 9e-06
Identities = 64/252 (25%), Positives = 104/252 (41%), Gaps = 32/252 (12%)
Frame = +3
Query: 3315 ADVCDVEADNEIKQKHTTDEQMGTDIQEKEAIMDSVSAQQMPEEKSTSEDRD-------- 3470
A+V ++ ++ ++ +K E ++ E + +S PE +ED D
Sbjct: 2 AEVLNLVSNEQVNEK----ESSSAELTEDKDYSHKLSVVLEPEVNQPAEDTDRLQLAEGA 57
Query: 3471 --QEKPG-------------DHFL-EEGIAPEDVCDLKAXXXXXXXXXXXXXXXXXXXXQ 3602
+EK DH L EEG + CDL++ Q
Sbjct: 58 SHEEKQQTDLHDLPVSRFLMDHILREEGDSLNKACDLESKDKIHEENDGGEQNEVEIPKQ 117
Query: 3603 EVNMSPLSAKEIPKDEYTD-------KVEVVSDVTNEEQNHETKADDEKPIAKDDESLHS 3761
E + LSA+++ ++ K+E S + E+Q+H+ K + E P+ KDD + S
Sbjct: 118 EGILVSLSAEQVTEETSISEDSVGHKKLENSSHLVQEQQSHDVKENTEIPVVKDDVTPQS 177
Query: 3762 NICLSSTTTA-SEPVEIEVPGSLAGESASVVMDDYKGVTPMDQGDLGSDAGAEELTGRTS 3938
++ SS T A E ++ EV L+ S VV +D +T +Q G EE
Sbjct: 178 SLHDSSPTVAVGEDIKDEVIRLLSEVSTHVVPNDSMELTSTEQNTPGIKVDTEECMDPGL 237
Query: 3939 VQDEKIIASPTE 3974
VQDE I A+ E
Sbjct: 238 VQDEIIQATVRE 249
>ref|XP_004079613.1| PREDICTED: uncharacterized protein LOC101155885 [Oryzias latipes]
Length = 1066
Score = 88.2 bits (217), Expect = 2e-14
Identities = 94/478 (19%), Positives = 203/478 (42%), Gaps = 9/478 (1%)
Frame = +3
Query: 2742 NVADEGIANGGIPDIPELAQASAEDTIDTEIKIKVAEKEAIAEK---GSTSANDTEDKDS 2912
NV +E + G + + L + E ++ I+ + E+E + EK G+ + E+++
Sbjct: 200 NVEEENVEEGNVEE-ENLKENVEEGNVEKNIEEENVEEENVGEKMQEGNVKEENVEEEN- 257
Query: 2913 PVSSSFTQATQVALPRDANEVVEDNAKRSHGVEES---DNSQHEESLQSDPADLPVSRFL 3083
V + V V E+N + + EE+ +N + E + + + V + +
Sbjct: 258 -VKEENVEEENVEEENLKENVKEENVEEENVEEENVEEENVEEENVEEENVEEENVEKNI 316
Query: 3084 MDHILHQGDVPDEVGNDESKNNIEEETTSDKLMTISIQEQEIFMDRLSAELVPSENIVEN 3263
+ + + +V +E +E N+EEE ++ + +QE + + + E V EN+ EN
Sbjct: 317 EEENVEEENVEEENVKEE---NVEEENVKEENVGEKMQEGNVKEENVEEENVEEENLKEN 373
Query: 3264 KGMEKLEDTILQQDNMPADVCDVEADNEIKQKHTTDEQMGTDIQEKEAIMDSVSAQQMPE 3443
E +E+ ++++N+ +V + + +++++ +E + + E+E + ++V + + E
Sbjct: 374 VKEENVEEENVEEENLKENVKENVEEENVEEENVKEENVEEENVEEENLKENVKEENVEE 433
Query: 3444 EKSTSEDRDQEKPGDHFLEEGIAPEDVCDLKAXXXXXXXXXXXXXXXXXXXXQEVNMSPL 3623
E E+ ++E + +EE E+ K +E N+
Sbjct: 434 ENVEEENVEEENVKEENVEEENVKEENVGEKMQEGNVKEENVEEENVKEENVEEENVEEE 493
Query: 3624 SAKEIPKDEYTDKVEVVSDVTNEEQNHETKADDEKPIAK---DDESLHSNICLSSTTTAS 3794
+ KE K+E ++ E V + EE+N E + +E+ + + ++E+L N+ +
Sbjct: 494 NLKENVKEENVEE-ENVEEENVEEENVEEENVEEENVEEGNVEEENLKENVEEGNVEKNI 552
Query: 3795 EPVEIEVPGSLAGESASVVMDDYKGVTPMDQGDLGSDAGAEELTGRTSVQDEKIIASPTE 3974
E +E GE M +G++ + EE +V++E + E
Sbjct: 553 EEENVEEEN--VGEK-------------MQEGNVKEENVEEENVKEENVEEENV----EE 593
Query: 3975 TEIRENLTQMDNYSPATNASNVSIPRDGENTRTFDNVEQIEKEAHSISEEQITSAISE 4148
++EN+ + NV E +NVE+ E ++ EE + I E
Sbjct: 594 ENLKENVKE----------ENVEEENVEEENVEEENVEEENVEEENVEEENVEKNIEE 641
Score = 71.2 bits (173), Expect = 2e-09
Identities = 75/387 (19%), Positives = 166/387 (42%), Gaps = 1/387 (0%)
Frame = +3
Query: 2973 VVEDNAKRSHGVEESDNSQH-EESLQSDPADLPVSRFLMDHILHQGDVPDEVGNDESKNN 3149
V E+N + + EE+ ++ EE++Q + V ++ + + +V +E +E++ N
Sbjct: 107 VEEENVEEGNVEEENVEEENVEENMQEGNVEENVQEGNVEKNVEEENVEEENVEEENEEN 166
Query: 3150 IEEETTSDKLMTISIQEQEIFMDRLSAELVPSENIVENKGMEKLEDTILQQDNMPADVCD 3329
+EEE + + +++E+ + + + E V EN+ E E +E+ ++++N+ +V +
Sbjct: 167 VEEENLKENVKEENVEEENVEEENVEEENVEEENVEE----ENVEEGNVEEENLKENVEE 222
Query: 3330 VEADNEIKQKHTTDEQMGTDIQEKEAIMDSVSAQQMPEEKSTSEDRDQEKPGDHFLEEGI 3509
+ I++++ +E +G +QE ++V + + EE E+ ++E ++ EE +
Sbjct: 223 GNVEKNIEEENVEEENVGEKMQEGNVKEENVEEENVKEENVEEENVEEENLKENVKEENV 282
Query: 3510 APEDVCDLKAXXXXXXXXXXXXXXXXXXXXQEVNMSPLSAKEIPKDEYTDKVEVVSDVTN 3689
E+V +E N+ + +E E V +
Sbjct: 283 EEENV-------------------------EEENVEEENVEE----------ENVEEENV 307
Query: 3690 EEQNHETKADDEKPIAKDDESLHSNICLSSTTTASEPVEIEVPGSLAGESASVVMDDYKG 3869
EE+N E ++E +++ N+ E V+ E G E V ++
Sbjct: 308 EEENVEKNIEEEN--VEEENVEEENV--KEENVEEENVKEENVGEKMQEGN--VKEENVE 361
Query: 3870 VTPMDQGDLGSDAGAEELTGRTSVQDEKIIASPTETEIRENLTQMDNYSPATNASNVSIP 4049
+++ +L + EE +V++E + + E EN+ + + NV
Sbjct: 362 EENVEEENLKENV-KEENVEEENVEEENLKENVKENVEEENVEEENVKEENVEEENVEEE 420
Query: 4050 RDGENTRTFDNVEQIEKEAHSISEEQI 4130
EN + +NVE+ E ++ EE +
Sbjct: 421 NLKENVKE-ENVEEENVEEENVEEENV 446
Score = 69.7 bits (169), Expect = 7e-09
Identities = 53/266 (19%), Positives = 126/266 (47%), Gaps = 6/266 (2%)
Frame = +3
Query: 2742 NVADEGIANGGIPDIPELAQASAEDTIDTEIKIKVAEKEAIAEK---GSTSANDTEDKDS 2912
NV +E + G + + L + E ++ I+ + E+E + EK G+ + E+++
Sbjct: 523 NVEEENVEEGNVEE-ENLKENVEEGNVEKNIEEENVEEENVGEKMQEGNVKEENVEEEN- 580
Query: 2913 PVSSSFTQATQVALPRDANEVVEDNAKRSHGVEES---DNSQHEESLQSDPADLPVSRFL 3083
V + V V E+N + + EE+ +N + E + + + V + +
Sbjct: 581 -VKEENVEEENVEEENLKENVKEENVEEENVEEENVEEENVEEENVEEENVEEENVEKNI 639
Query: 3084 MDHILHQGDVPDEVGNDESKNNIEEETTSDKLMTISIQEQEIFMDRLSAELVPSENIVEN 3263
+ + + +V +E +E N+EEE ++ + +QE + + + E V EN+ EN
Sbjct: 640 EEENVEEENVEEENVKEE---NVEEENVKEENVGEKMQEGNVKEENVEEENVEEENLKEN 696
Query: 3264 KGMEKLEDTILQQDNMPADVCDVEADNEIKQKHTTDEQMGTDIQEKEAIMDSVSAQQMPE 3443
E +E+ ++++N+ +V + + +++++ +E + + E+E + ++V + + E
Sbjct: 697 VKEENVEEENVEEENLKENVKENVEEENVEEENVKEENVEEENVEEENLKENVKEENVEE 756
Query: 3444 EKSTSEDRDQEKPGDHFLEEGIAPED 3521
E E+ ++E + +EE E+
Sbjct: 757 ENVEEENVEEENVKEENVEEENVKEE 782
Score = 65.5 bits (158), Expect = 1e-07
Identities = 76/437 (17%), Positives = 171/437 (39%), Gaps = 14/437 (3%)
Frame = +3
Query: 2742 NVADEGIANGGIPDIPELAQASAEDTIDTEIKIKVAEKEAIAEKGSTSANDTEDK----- 2906
NV +E + + + + E+ + ++ + E+E + E+ N E+
Sbjct: 484 NVEEENVEEENLKENVKEENVEEENVEEENVEEENVEEENVEEENVEEGNVEEENLKENV 543
Query: 2907 -----DSPVSSSFTQATQVALPRDANEVVEDNAKRSHGVEES--DNSQHEESLQSDPADL 3065
+ + + V V E+N + + EE+ + + EE+L+ + +
Sbjct: 544 EEGNVEKNIEEENVEEENVGEKMQEGNVKEENVEEENVKEENVEEENVEEENLKENVKEE 603
Query: 3066 PVSRFLMDHILHQGDVPDEVGNDES--KNNIEEETTSDKLMTISIQEQEIFMDRLSAELV 3239
V + + + +V +E +E+ + N+EEE + +++E+ + + + E V
Sbjct: 604 NVE----EENVEEENVEEENVEEENVEEENVEEENVEKNIEEENVEEENVEEENVKEENV 659
Query: 3240 PSENIVENKGMEKLEDTILQQDNMPADVCDVEADNEIKQKHTTDEQMGTDIQEKEAIMDS 3419
EN+ E EK+++ ++++N+ + + E E ++ +E+ + KE + ++
Sbjct: 660 EEENVKEENVGEKMQEGNVKEENVEEENVEEENLKENVKEENVEEENVEEENLKENVKEN 719
Query: 3420 VSAQQMPEEKSTSEDRDQEKPGDHFLEEGIAPEDVCDLKAXXXXXXXXXXXXXXXXXXXX 3599
V + + EE E+ ++E + L+E + E+V
Sbjct: 720 VEEENVEEENVKEENVEEENVEEENLKENVKEENV--------------------EEENV 759
Query: 3600 QEVNMSPLSAKEIPKDEYTDKVEVVSDVTNEEQNHETKADDEKPIAKDDESLHSNICLSS 3779
+E N+ + KE +E K E V + E E ++E K++ N+
Sbjct: 760 EEENVEEENVKEENVEEENVKEENVGEKMQEGNVKEENVEEENENVKEENVEEENV--EE 817
Query: 3780 TTTASEPVEIEVPGSLAGESASVVMDDYKGVTPMDQGDLGSDAGAEELTGRTSVQDEKII 3959
E VE E E +V ++ + +G++ + EE +V++ +
Sbjct: 818 ENVEEENVEEENVEEENVEEGNVEEEN------LQEGNVEEENVEEENVEEENVEEGNMQ 871
Query: 3960 ASPTETEIRENLTQMDN 4010
E + E Q N
Sbjct: 872 EGNVEKNVEEENVQEGN 888
>ref|XP_002490879.1| Mucin-like protein [Komagataella pastoris GS115]
gi|238030675|emb|CAY68599.1| Mucin-like protein
[Komagataella pastoris GS115]
Length = 1416
Score = 81.3 bits (199), Expect = 2e-12
Identities = 110/506 (21%), Positives = 203/506 (40%), Gaps = 20/506 (3%)
Frame = +3
Query: 2730 TDGNNVADEGIANGGIPDIPELAQASAEDTIDTEIKIKVAEKEAIAEK--GSTSANDTED 2903
TD +V++ A P E A+ SAE++ TE + E A E+ STS D E+
Sbjct: 438 TDSQSVSESSAAEDSTPT--EEAEESAEESTSTEDAEESTEDFATTEEVEESTSTEDAEE 495
Query: 2904 KDSPVSSSFTQATQVALPRDANEVVEDNAKRSHG---VEESDNSQH-----EESLQSDPA 3059
S + + +T+ A + E E++ + S VEES +++ EES ++ A
Sbjct: 496 STSTEEAEESTSTEDAEESTSTEEAEESTEESTSTDEVEESTSTEEVEESTEESTSTEDA 555
Query: 3060 DLPVSRFLMDHILHQGDVPDEVGNDESKNNIE---EETTSDKLMTISIQEQEIFMDRLSA 3230
+ S + + DEV S +E EE+TS + S +E+ + +
Sbjct: 556 EESTSTEEAEESTEESTSTDEVEESTSTEEVEESTEESTSTDEVEESTSTEEV--EESTE 613
Query: 3231 ELVPSENIVENKGMEKLEDTILQQDNMPADVCDVEADNEIKQKHTTDEQMGTDIQEKEAI 3410
E ++ + E+ E++E++ ++ D + + E ++ +T+E TD E +
Sbjct: 614 ESTSTDEVEESTSTEEVEES-TEESTSTEDAEESTSTEEAEE--STEESTSTD--EVDES 668
Query: 3411 MDSVSAQQMPEEKSTSEDRDQE---KPGDHFLEEGIAPEDVCDLKAXXXXXXXXXXXXXX 3581
+ A++ EE +++ED ++ + + EE + E+
Sbjct: 669 TSTEEAEESTEESTSTEDAEESTSTEEAEESTEESTSTEET------------EESTEEL 716
Query: 3582 XXXXXXQEVNMSPLSAKEIPKDEYTDKVEVVSDVTNEEQNHETKADDEKP----IAKDDE 3749
+E P S E+ + TD V+ + EQ T +P ++ E
Sbjct: 717 TSTEEAEESTEEPTSTDEVDESTSTDDVDESTSTEGTEQFSSTDVPQGRPGFENPTEEVE 776
Query: 3750 SLHSNICLSSTTTASEPVEIEVPGSLAGESASVVMDDYKGVTPMDQGDLGSDAGAEELTG 3929
S + T+T E S S+ DD + T +++ ++ EE T
Sbjct: 777 SSSTEEFEEPTSTDETDESTEEATSTEEAEESISTDDVEQSTSVEE----AEESTEESTS 832
Query: 3930 RTSVQDEKIIASPTETEIRENLTQMDNYSPATNASNVSIPRDGENTRTFDNVEQIEKEAH 4109
++++ T T EN++ +D + + S E+T T D E E
Sbjct: 833 TEALEES------TSTGDFENISAVDEELEESTEESTSTEEVEESTSTEDAEESTSTEEA 886
Query: 4110 SISEEQITSAISELLGSTTTTVRSEE 4187
S E+ TS +E +T+T +EE
Sbjct: 887 EESTEESTS--TEDAEESTSTEEAEE 910
>emb|CCA37660.1| Cell surface glycoprotein 1 [Komagataella pastoris CBS 7435]
Length = 1618
Score = 75.1 bits (183), Expect = 2e-10
Identities = 112/505 (22%), Positives = 190/505 (37%), Gaps = 19/505 (3%)
Frame = +3
Query: 2730 TDGNNVADEGIANGGIPDIPELAQASAEDTIDTEIKIKVAEKEAIAEKGSTSANDTEDKD 2909
TD +V++ A P E A+ SAE++ TE + E A E+ S T +D
Sbjct: 438 TDSQSVSESSAAEDSTPT--EEAEESAEESTSTEDAEESTEDFATTEEVEES---TSTED 492
Query: 2910 SPVSSSFTQATQVALPRDANEVVEDNAKRSHGVEESDNSQHEESLQSDPADLPVSRFLMD 3089
+ S+S +A + DA E S EE++ S EES +D + S ++
Sbjct: 493 AEESTSTEEAEESTSTEDAEE--------STSTEEAEEST-EESTSTDEVEESTSTEEVE 543
Query: 3090 HILHQGDVPDEVGNDESKNNIEEET-----TSDKLMTISIQEQEIFMDRLSAELVPSENI 3254
+ DEV S +EE T T D + S +E E + E ++ +
Sbjct: 544 ESTEESTSTDEVEESTSTEEVEESTEESTSTEDAEESTSTEEAE----ESTEESTSTDEV 599
Query: 3255 VENKGMEKLEDTILQQ---DNMPADVCDVEADNEIKQKHTTD--EQMGTDIQEKEAIMDS 3419
E+ E+ E++ + D + E + I++ +T+ E+ ++ E +S
Sbjct: 600 DESTSTEEAEESTEESTSTDEVEESTSTEEVEESIEESTSTEEPEESTEELTSTEEAEES 659
Query: 3420 VSAQQMPE--EKSTS----EDRDQEKPGDHFLEEGIAPEDVCDLKAXXXXXXXXXXXXXX 3581
S ++ E E+STS E+ +E +EE + E+V +
Sbjct: 660 TSTDEVDESTEESTSTEEAEESTEESTSTDEVEESTSTEEV---EESTEESTSTEDAEES 716
Query: 3582 XXXXXXQEVNMSPLSAKEIPKDEYTDKVEVVSDVTNEEQNHETKADDEKPIAKDDESLHS 3761
+E S E+ + T++VE E T +D + +E+ S
Sbjct: 717 TSTEEAEESTEESTSTDEVEESTSTEEVE-------ESTEESTSTEDAEESTSTEEAEES 769
Query: 3762 NICLSSTTTASEPVEIEVPGSLAGESASVVMDDYKGVTPMDQGDLGSDAGAEELTGRTSV 3941
+ST E E ES S + T + +E+ TS
Sbjct: 770 TEESTSTDEVEESTSTEEVEESTEESTSTDEVEESTSTEEVEESTEESTSTDEVEESTST 829
Query: 3942 QD-EKIIASPTETEIRENLTQMDNYSPATNASNVSIPRDGENTRTFDNVEQIEKEAHSIS 4118
++ E+ T TE E T + +T S S E+T T + E E+ +
Sbjct: 830 EEVEESTEESTSTEDAEESTSTEEAEESTEES-TSTDEVDESTSTEEAEESTEESTSTED 888
Query: 4119 EEQITSA--ISELLGSTTTTVRSEE 4187
E+ TS E +T+T +EE
Sbjct: 889 AEESTSTEEAEESTEESTSTEETEE 913
>ref|XP_002259727.1| hypothetical protein, conserved in Plasmodium species [Plasmodium
knowlesi strain H] gi|193809799|emb|CAQ40503.1|
hypothetical protein, conserved in Plasmodium species
[Plasmodium knowlesi strain H]
Length = 2758
Score = 73.2 bits (178), Expect = 6e-10
Identities = 73/287 (25%), Positives = 116/287 (40%), Gaps = 22/287 (7%)
Frame = +1
Query: 364 DKMQKNESPMLDVDEEHDNESSEVNDILENKLSDDD-------GAISGPSSEEKVASSVF 522
D ++K ES DV+EE+DN+ E D +N DDD G + EE+V S V
Sbjct: 2238 DGVEKEES---DVEEENDNDDDEEEDEEDNDDDDDDDDGNEDEGEVDSGVDEEEVESDVH 2294
Query: 523 EVVGSTKLGICLNQSEVETETISLDDNKDEIIPKDEETLYQTSDSSTVAENLGTPINSES 702
EV ++ DDN D+ DE+ ++ + V G E
Sbjct: 2295 EVESGEEVD---------------DDNNDDEYDDDEDDEEESVEVDQVESLEGEEDEDEG 2339
Query: 703 RKLTIN-EGGGSDATDIVNVEPKMVDEGCEDNEEKKFEEVATKSDDRDQYSEISKATVTT 879
++ E + D + E + +EG ED+EE +EE D D+ E +A
Sbjct: 2340 EGSAVDVEQDEDEEEDDADEEEEDEEEGGEDDEEGDYEE-----GDDDEEEEEEEAVEEE 2394
Query: 880 KDADSHEQIKDSNIPNDRRDAERKADTET--EKISSAEAETEPALEEDKIVITPQG---- 1041
+ + E+ +D D D E D E E+ S E E E E+ I I QG
Sbjct: 2395 EQEEEEEEEEDEEEEED-EDEEEDEDEEVVEEEESGIEVEEEDETTEEDIEIEGQGESDV 2453
Query: 1042 --------DLEDTTINAKEETETKLDTIHPSSIMVDQEDFRNQKDNQ 1158
D+E+ I +EE + + + V+ +D ++D++
Sbjct: 2454 DVEGEEEEDVEEEEIEVEEEVGSSEEEVEEDEADVEDDDEDEEEDDE 2500