BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00000077
         (1650 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002528344.1| 3-isopropylmalate dehydratase, putative [Ric...   887   0.0  
ref|XP_002453237.1| hypothetical protein SORBIDRAFT_04g002120 [S...   878   0.0  
ref|XP_002271046.1| PREDICTED: 3-isopropylmalate dehydratase-lik...   878   0.0  
ref|NP_001045743.1| Os02g0125100 [Oryza sativa Japonica Group] g...   878   0.0  
gb|ACR38517.1| unknown [Zea mays] gi|413935380|gb|AFW69931.1| 3-...   869   0.0  

>ref|XP_002528344.1| 3-isopropylmalate dehydratase, putative [Ricinus communis]
            gi|223532212|gb|EEF34016.1| 3-isopropylmalate
            dehydratase, putative [Ricinus communis]
          Length = 510

 Score =  887 bits (2293), Expect = 0.0
 Identities = 431/507 (85%), Positives = 463/507 (91%), Gaps = 3/507 (0%)
 Frame = +2

Query: 107  MATIAMTLSRVHFGGKSELGVS---SLFSPSIIAHRCRRQASNKIHAVMTPTQSQRPLST 277
            MA+ A+T     F  K +LG+S   S  SP     +C++  S K+ +VM P QS+R  +T
Sbjct: 1    MASSAITSPSSSFITKKDLGLSAFSSQSSPFYSIPKCKKSISKKVVSVMAPQQSERTPAT 60

Query: 278  TGSVKHGMTMTEKILSRASERSQLEPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGENAK 457
            TGSVK  MTMTEKIL+RASE+SQL PGENVWVNVDVLMTHDVCGPG+ GIFK+EFG+NA 
Sbjct: 61   TGSVKTAMTMTEKILARASEKSQLSPGENVWVNVDVLMTHDVCGPGSIGIFKREFGQNAT 120

Query: 458  VWDREKIVIIPDHYIFTNDERANRNVDILRDFCMEQNIKYFYDIKDLGNFKANPDYKGVC 637
            VWDREKIVIIPDHYIFT DERANRNVDILRDFC EQN+KYFYDIKDL NFK NPDYKGVC
Sbjct: 121  VWDREKIVIIPDHYIFTTDERANRNVDILRDFCQEQNVKYFYDIKDLSNFKVNPDYKGVC 180

Query: 638  HIALAQEGHCRPGEVLVGTDSHTCNAGAFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRF 817
            H+ALAQEGHCRPGEVL+GTDSHTC AGAFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRF
Sbjct: 181  HVALAQEGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRF 240

Query: 818  ILDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFIGSTVESLSMEERMTLCNMVVEAGG 997
            ++DGEMPDYLLAKDLILQIIGEISVSGATYKSMEF+G+TVESL+MEERMTLCNMVVEAGG
Sbjct: 241  VMDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFVGTTVESLNMEERMTLCNMVVEAGG 300

Query: 998  KNGVVPADQTTFNYLKDKTSVDFEPVYSDGNARFLQDYKFDVSKLEPLVAKPHSPDNRAL 1177
            KNGV+P+D TTF YL+DKTSV FEPVYSD  ARFL +Y+FDVSKLEPLVAKPHSPDN AL
Sbjct: 301  KNGVIPSDSTTFKYLEDKTSVPFEPVYSDEKARFLSEYRFDVSKLEPLVAKPHSPDNCAL 360

Query: 1178 ARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLP 1357
            ARECKDVKIDRVYIGSCTGGKTEDF+AAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLP
Sbjct: 361  ARECKDVKIDRVYIGSCTGGKTEDFMAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLP 420

Query: 1358 VPGSGGKTCSQIFEEAGCDTPASPNCGACLGGPRDTYARMNEPMVCVSTTNRNFPGRMGH 1537
            VPGSGGKTCSQIFEEAGCDTPASP+CGACLGGP+DTYARMNEPMVCVSTTNRNFPGRMGH
Sbjct: 421  VPGSGGKTCSQIFEEAGCDTPASPSCGACLGGPKDTYARMNEPMVCVSTTNRNFPGRMGH 480

Query: 1538 KEGQIYLASPYTAAASALTGYVTDPRE 1618
            KEGQIYLASPYTAAASALTGYVTDPRE
Sbjct: 481  KEGQIYLASPYTAAASALTGYVTDPRE 507


>ref|XP_002453237.1| hypothetical protein SORBIDRAFT_04g002120 [Sorghum bicolor]
            gi|241933068|gb|EES06213.1| hypothetical protein
            SORBIDRAFT_04g002120 [Sorghum bicolor]
          Length = 507

 Score =  878 bits (2269), Expect = 0.0
 Identities = 423/484 (87%), Positives = 452/484 (93%), Gaps = 3/484 (0%)
 Frame = +2

Query: 176  LFSPSIIAHRC---RRQASNKIHAVMTPTQSQRPLSTTGSVKHGMTMTEKILSRASERSQ 346
            L +P+   HR    RR    ++ AV +P ++ R  S+TGSVK  MTMTEKIL+RASER+ 
Sbjct: 21   LAAPAPAQHRAGSSRRTKPCRVRAVASPARAPRAPSSTGSVKSAMTMTEKILARASERAA 80

Query: 347  LEPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGENAKVWDREKIVIIPDHYIFTNDERAN 526
            LEPGENVWV+VDVLMTHDVCGPGT GIFKKEFGE+AKVWDREK+VIIPDHYIFT DERAN
Sbjct: 81   LEPGENVWVDVDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTTDERAN 140

Query: 527  RNVDILRDFCMEQNIKYFYDIKDLGNFKANPDYKGVCHIALAQEGHCRPGEVLVGTDSHT 706
            RNVDILRDFC+EQNIKYFYDIKDL NFKANPDYKGVCH+ALAQEGHCRPGEVL+GTDSHT
Sbjct: 141  RNVDILRDFCLEQNIKYFYDIKDLSNFKANPDYKGVCHVALAQEGHCRPGEVLLGTDSHT 200

Query: 707  CNAGAFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRFILDGEMPDYLLAKDLILQIIGEI 886
            CNAGAFGQFATGIGNTDAGFVLGTGK LLKVPPT+RF+LDGEMP YLLAKDLILQIIGEI
Sbjct: 201  CNAGAFGQFATGIGNTDAGFVLGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEI 260

Query: 887  SVSGATYKSMEFIGSTVESLSMEERMTLCNMVVEAGGKNGVVPADQTTFNYLKDKTSVDF 1066
            SVSGATYKSMEF+GSTVESL+MEERMTLCNMV+EAGGKNGVVPAD+TTF YL+ KTSVD+
Sbjct: 261  SVSGATYKSMEFVGSTVESLTMEERMTLCNMVIEAGGKNGVVPADETTFKYLEGKTSVDY 320

Query: 1067 EPVYSDGNARFLQDYKFDVSKLEPLVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTE 1246
            EPVYSD  ARF  DY+FDVSKLEP+VAKPHSPDNRALARECKDVK+DRVYIGSCTGGKTE
Sbjct: 321  EPVYSDAQARFFSDYRFDVSKLEPVVAKPHSPDNRALARECKDVKVDRVYIGSCTGGKTE 380

Query: 1247 DFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPAS 1426
            DFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPAS
Sbjct: 381  DFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPAS 440

Query: 1427 PNCGACLGGPRDTYARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVT 1606
            P+CGACLGGPRDTYARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVT
Sbjct: 441  PSCGACLGGPRDTYARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVT 500

Query: 1607 DPRE 1618
            DPR+
Sbjct: 501  DPRD 504


>ref|XP_002271046.1| PREDICTED: 3-isopropylmalate dehydratase-like [Vitis vinifera]
          Length = 508

 Score =  878 bits (2269), Expect = 0.0
 Identities = 426/491 (86%), Positives = 454/491 (92%), Gaps = 2/491 (0%)
 Frame = +2

Query: 152  KSELGVSSLFSP--SIIAHRCRRQASNKIHAVMTPTQSQRPLSTTGSVKHGMTMTEKILS 325
            K ++G+S+  S   S    + +R+ S KI +VM P QS R  STTGSVK  MTMTEKIL+
Sbjct: 15   KKDVGLSAFSSSPSSFSVPKTKRRNSRKIVSVMAPQQSPRSPSTTGSVKTAMTMTEKILA 74

Query: 326  RASERSQLEPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGENAKVWDREKIVIIPDHYIF 505
            RAS++ QL PGENVWV+VD+LMTHDVCGPG+FGIFKKEFG+NAKVWDREKIVIIPDHYIF
Sbjct: 75   RASQKPQLSPGENVWVDVDILMTHDVCGPGSFGIFKKEFGQNAKVWDREKIVIIPDHYIF 134

Query: 506  TNDERANRNVDILRDFCMEQNIKYFYDIKDLGNFKANPDYKGVCHIALAQEGHCRPGEVL 685
            TNDERANRNVDILRDFC EQNIKYFYDIKDLGNFK NPDYKGVCH+ALAQEGHCRPGEVL
Sbjct: 135  TNDERANRNVDILRDFCTEQNIKYFYDIKDLGNFKVNPDYKGVCHVALAQEGHCRPGEVL 194

Query: 686  VGTDSHTCNAGAFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRFILDGEMPDYLLAKDLI 865
            +GTDSHTC AGAFGQFATGIGNTDAGFVLGTGK+LLKVPPTLRF++DGEMPDYLLAKDLI
Sbjct: 195  LGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTLRFVMDGEMPDYLLAKDLI 254

Query: 866  LQIIGEISVSGATYKSMEFIGSTVESLSMEERMTLCNMVVEAGGKNGVVPADQTTFNYLK 1045
            LQIIGEISVSGATYKSMEF+GSTVESL+MEERMTLCNMVVEAGGKNGVVPAD TT  YL+
Sbjct: 255  LQIIGEISVSGATYKSMEFVGSTVESLTMEERMTLCNMVVEAGGKNGVVPADSTTHKYLE 314

Query: 1046 DKTSVDFEPVYSDGNARFLQDYKFDVSKLEPLVAKPHSPDNRALARECKDVKIDRVYIGS 1225
            DKT V +EPVYSD  ARFL +Y+FDVSKLEPLVAKPHSPDNRALARECKDVKIDRVYIGS
Sbjct: 315  DKTDVPYEPVYSDEQARFLSEYRFDVSKLEPLVAKPHSPDNRALARECKDVKIDRVYIGS 374

Query: 1226 CTGGKTEDFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEA 1405
            CTGGKTEDFLAAAKVF ASGKKVKVPTFLVPATQKVWMDVYSLPVPG GGKTCSQIFEEA
Sbjct: 375  CTGGKTEDFLAAAKVFHASGKKVKVPTFLVPATQKVWMDVYSLPVPGCGGKTCSQIFEEA 434

Query: 1406 GCDTPASPNCGACLGGPRDTYARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPYTAAAS 1585
            GCDTPASP+CGACLGGPRDTYARMNEP VCVSTTNRNFPGRMGHKEGQIYLASPYTAAAS
Sbjct: 435  GCDTPASPSCGACLGGPRDTYARMNEPQVCVSTTNRNFPGRMGHKEGQIYLASPYTAAAS 494

Query: 1586 ALTGYVTDPRE 1618
            ALTG+VTDPRE
Sbjct: 495  ALTGFVTDPRE 505


>ref|NP_001045743.1| Os02g0125100 [Oryza sativa Japonica Group]
            gi|41053038|dbj|BAD07969.1| putative 3-isopropylmalate
            dehydratase large subunit [Oryza sativa Japonica Group]
            gi|113535274|dbj|BAF07657.1| Os02g0125100 [Oryza sativa
            Japonica Group] gi|215678837|dbj|BAG95274.1| unnamed
            protein product [Oryza sativa Japonica Group]
            gi|218189963|gb|EEC72390.1| hypothetical protein
            OsI_05669 [Oryza sativa Indica Group]
            gi|222622087|gb|EEE56219.1| hypothetical protein
            OsJ_05201 [Oryza sativa Japonica Group]
          Length = 514

 Score =  878 bits (2268), Expect = 0.0
 Identities = 416/470 (88%), Positives = 449/470 (95%)
 Frame = +2

Query: 209  RRQASNKIHAVMTPTQSQRPLSTTGSVKHGMTMTEKILSRASERSQLEPGENVWVNVDVL 388
            RR  S ++ AV TP ++ R  S+TGSVK  MTMTEKIL+RASER+ LEPGENVWV+VDVL
Sbjct: 42   RRARSGRVRAVATPARAPRAPSSTGSVKSAMTMTEKILARASERASLEPGENVWVDVDVL 101

Query: 389  MTHDVCGPGTFGIFKKEFGENAKVWDREKIVIIPDHYIFTNDERANRNVDILRDFCMEQN 568
            MTHDVCGPGT GIFK+EFGE+AKVWDREK+VIIPDHYIFT+DERANRNVDILRDFCMEQN
Sbjct: 102  MTHDVCGPGTIGIFKREFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQN 161

Query: 569  IKYFYDIKDLGNFKANPDYKGVCHIALAQEGHCRPGEVLVGTDSHTCNAGAFGQFATGIG 748
            IKYFYDIKDL NFKANPDYKGVCH+ALAQEGHCRPGEVL+GTDSHTCNAGAFGQFATGIG
Sbjct: 162  IKYFYDIKDLSNFKANPDYKGVCHVALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIG 221

Query: 749  NTDAGFVLGTGKLLLKVPPTLRFILDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFIG 928
            NTDAGFV+GTGK LLKVPPT+RF+LDGEMP YLLAKDLILQIIGEISVSGATYKSMEF+G
Sbjct: 222  NTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVG 281

Query: 929  STVESLSMEERMTLCNMVVEAGGKNGVVPADQTTFNYLKDKTSVDFEPVYSDGNARFLQD 1108
            STVESL+MEERMTLCNMV+EAGGKNGVVPADQTTFNYL+ KTSV++EPVYSD  ARF+ D
Sbjct: 282  STVESLNMEERMTLCNMVIEAGGKNGVVPADQTTFNYLEGKTSVEYEPVYSDAQARFVSD 341

Query: 1109 YKFDVSKLEPLVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGK 1288
            Y+FDVSKLEP++AKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDF AAAKVFLASGK
Sbjct: 342  YRFDVSKLEPVIAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFFAAAKVFLASGK 401

Query: 1289 KVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPASPNCGACLGGPRDTY 1468
            KVKVPTFLVPATQKVWMD+YS+PVPG+GGKTCSQIFEEAGCDTPASP+CGACLGGPRDTY
Sbjct: 402  KVKVPTFLVPATQKVWMDIYSIPVPGAGGKTCSQIFEEAGCDTPASPSCGACLGGPRDTY 461

Query: 1469 ARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVTDPRE 1618
            ARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASP+TAAASALTGYVTDPR+
Sbjct: 462  ARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPFTAAASALTGYVTDPRD 511


>gb|ACR38517.1| unknown [Zea mays] gi|413935380|gb|AFW69931.1| 3-isopropylmalate
            dehydratase large subunit 2 isoform 1 [Zea mays]
            gi|413935381|gb|AFW69932.1| 3-isopropylmalate dehydratase
            large subunit 2 isoform 2 [Zea mays]
          Length = 505

 Score =  869 bits (2246), Expect = 0.0
 Identities = 415/470 (88%), Positives = 446/470 (94%)
 Frame = +2

Query: 209  RRQASNKIHAVMTPTQSQRPLSTTGSVKHGMTMTEKILSRASERSQLEPGENVWVNVDVL 388
            RR    ++ AV +P ++ R  S+TGSVK  MTMTEKIL+RASER+ LEPGENVWV++DVL
Sbjct: 33   RRTKPCRVRAVASPARTPRAPSSTGSVKTAMTMTEKILARASERAALEPGENVWVDIDVL 92

Query: 389  MTHDVCGPGTFGIFKKEFGENAKVWDREKIVIIPDHYIFTNDERANRNVDILRDFCMEQN 568
            MTHDVCGPGT GIFKKEFGE+AKVWDREK+VIIPDHYIFT+DERANRNVDILRDFC+EQN
Sbjct: 93   MTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCLEQN 152

Query: 569  IKYFYDIKDLGNFKANPDYKGVCHIALAQEGHCRPGEVLVGTDSHTCNAGAFGQFATGIG 748
            IKYFYDIKDL +F+ANPDYKGVCHIALAQEGHCRPGEVL+GTDSHTCNAGAFGQFATGIG
Sbjct: 153  IKYFYDIKDLSDFRANPDYKGVCHIALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIG 212

Query: 749  NTDAGFVLGTGKLLLKVPPTLRFILDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFIG 928
            NTDAGFV+GTGK LLKVPPT+RF+LDGEMP YLLAKDLILQIIGEISVSGATYKSMEF+G
Sbjct: 213  NTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVG 272

Query: 929  STVESLSMEERMTLCNMVVEAGGKNGVVPADQTTFNYLKDKTSVDFEPVYSDGNARFLQD 1108
            STVESL+MEERMTLCNMVVEAGGKNGVVPAD+TTF YL+ +TSVD++PVYSD  ARF  D
Sbjct: 273  STVESLTMEERMTLCNMVVEAGGKNGVVPADETTFKYLEGRTSVDYQPVYSDAEARFFSD 332

Query: 1109 YKFDVSKLEPLVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGK 1288
            Y+FDVSKLEP+VAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGK
Sbjct: 333  YRFDVSKLEPVVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGK 392

Query: 1289 KVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPASPNCGACLGGPRDTY 1468
            KVKVPTFLVPATQKVWMDVYSLPVPGSGGKTC+QIFEEAGCDTPASPNCGACLGGPRDTY
Sbjct: 393  KVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCAQIFEEAGCDTPASPNCGACLGGPRDTY 452

Query: 1469 ARMNEPMVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVTDPRE 1618
            ARMNEP VCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVTDPR+
Sbjct: 453  ARMNEPTVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVTDPRD 502