BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cornus23_contig00017677
         (838 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADU19849.1| putative secologanin synthase [Camptotheca acumin...   385   e-104
gb|AGX93044.1| secologanin synthase-like protein, partial [Lonic...   334   4e-89
sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Shor...   331   5e-88
gb|AGX93049.1| secologanin synthase-like protein [Vinca minor]        331   5e-88
gb|AAA17732.1| cytochrome P450 [Catharanthus roseus] gi|81676101...   330   8e-88
gb|AGX93046.1| secologanin synthase-like protein [Amsonia hubric...   330   1e-87
gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]         329   1e-87
gb|AGX93064.1| secologanin synthase [Catharanthus roseus] gi|589...   329   1e-87
gb|AGX93047.1| secologanin synthase-like protein [Rauvolfia serp...   328   2e-87
gb|AGX93048.1| secologanin synthase-like protein [Tabernaemontan...   328   4e-87
dbj|BAP90521.1| secologanin synthase [Ophiorrhiza pumila]             327   5e-87
gb|AKF02530.1| secologanin synthase-like protein 4 [Catharanthus...   325   3e-86
gb|AGX93045.1| secologanin synthase-like protein, partial [Cinch...   317   5e-84
ref|XP_011009102.1| PREDICTED: cytochrome P450 72A15-like isofor...   314   6e-83
ref|XP_006377681.1| cytochrome P450 family protein [Populus tric...   313   8e-83
ref|XP_006377693.1| hypothetical protein POPTR_0011s10300g [Popu...   313   1e-82
ref|XP_011009101.1| PREDICTED: cytochrome P450 CYP72A219-like [P...   311   3e-82
gb|AGX93056.1| 7-deoxyloganic acid 7-hydroxylase-like protein [L...   311   5e-82
ref|XP_006377690.1| cytochrome P450 family protein [Populus tric...   310   6e-82
ref|XP_010092744.1| Secologanin synthase [Morus notabilis] gi|58...   308   3e-81

>gb|ADU19849.1| putative secologanin synthase [Camptotheca acuminata]
          Length = 524

 Score =  385 bits (990), Expect = e-104
 Identities = 183/219 (83%), Positives = 204/219 (93%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ER G +K+AG+S+EDVISE KLF+FAGQETTGILLTWTCVLLSRHP WQERAREE+ 
Sbjct: 304 KEIERLGNKKNAGMSIEDVISECKLFYFAGQETTGILLTWTCVLLSRHPEWQERAREEIF 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           QVFGNGK DFDR+QNLKIV MILYEVLRLYPPVIELTKVT++E KLGNLTIPAGVQ+MMP
Sbjct: 364 QVFGNGKVDFDRVQNLKIVPMILYEVLRLYPPVIELTKVTYEEQKLGNLTIPAGVQLMMP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           SILLHRD +MWG D+KEFNPGRFA+GIS A KSP+FYIPFSWGPR+CVGQNFALLQAKMA
Sbjct: 424 SILLHRDQEMWGADSKEFNPGRFADGISKAVKSPFFYIPFSWGPRICVGQNFALLQAKMA 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           LT+ILQRF+F +SP+YAHAPFTVLTLQPQHGAQVVF K+
Sbjct: 484 LTMILQRFTFDLSPTYAHAPFTVLTLQPQHGAQVVFRKI 522


>gb|AGX93044.1| secologanin synthase-like protein, partial [Lonicera japonica]
          Length = 520

 Score =  334 bits (857), Expect = 4e-89
 Identities = 147/219 (67%), Positives = 189/219 (86%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E +R G +K+AG++++DVI E KLF+FAGQETTGILLTWT V+LS+HP WQ+RAREEV+
Sbjct: 302 KEIQRLGNKKNAGMTIDDVIEECKLFYFAGQETTGILLTWTMVMLSKHPEWQDRAREEVI 361

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
             FG  KPDF+R+  LK+V MILYEVLRLYPPVI+LTK+ H+ETKLG+L+IP GVQ+M+P
Sbjct: 362 SAFGKSKPDFERLNQLKVVGMILYEVLRLYPPVIDLTKIVHEETKLGDLSIPPGVQIMLP 421

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WGDDA EFNP RFA+G++NATK+   YIPFSWGPR+C+GQNFALLQAK+ 
Sbjct: 422 TVMLHREKKIWGDDAMEFNPSRFADGVANATKNNVSYIPFSWGPRVCIGQNFALLQAKLG 481

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF+F +SPSY HAP T+LTLQPQHGA V++ K+
Sbjct: 482 LAMILQRFTFDLSPSYTHAPMTILTLQPQHGAHVIYRKI 520


>sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Short=SLS; AltName:
           Full=CYPLXXII; AltName: Full=Cytochrome P450 72A1
           gi|167484|gb|AAA33106.1| cytochrome P-450 protein
           [Catharanthus roseus] gi|445604|prf||1909351A cytochrome
           P450 [Catharanthus roseus]
          Length = 524

 Score =  331 bits (848), Expect = 5e-88
 Identities = 146/219 (66%), Positives = 187/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG +KD G+S+ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEVL
Sbjct: 304 QEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVL 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KP+F+R+ +LK VSMILYEVLRLYPPVI+LTK+ HK+TKLG+ TIPAG QVM+P
Sbjct: 364 QAFGKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RF +G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVMLHREKSIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF F ++PSY HAPFT+LT+QPQ G+ V++ KL
Sbjct: 484 LAMILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522


>gb|AGX93049.1| secologanin synthase-like protein [Vinca minor]
          Length = 524

 Score =  331 bits (848), Expect = 5e-88
 Identities = 146/216 (67%), Positives = 187/216 (86%)
 Frame = -2

Query: 828 ERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQVF 649
           + QG +KD G+S+ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEVLQVF
Sbjct: 307 KNQGNKKDGGMSINDVIDECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQVF 366

Query: 648 GNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPSIL 469
           G  KP+FDR+ +LK +SMILYEVLRLYPPVI+LTK+ H++TKLG+ TIPAG QVM+P+++
Sbjct: 367 GKNKPEFDRLNHLKYISMILYEVLRLYPPVIDLTKIVHEDTKLGSYTIPAGAQVMLPTVM 426

Query: 468 LHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMALTL 289
           LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ L +
Sbjct: 427 LHREKSIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAM 486

Query: 288 ILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           ILQRF+F ++PSY HAPFT+LTLQPQ G+ V++ K+
Sbjct: 487 ILQRFTFDVAPSYVHAPFTILTLQPQLGSHVIYKKI 522


>gb|AAA17732.1| cytochrome P450 [Catharanthus roseus] gi|816761015|gb|AKF02529.1|
           secologanin synthase-like protein 3 [Catharanthus
           roseus]
          Length = 524

 Score =  330 bits (846), Expect = 8e-88
 Identities = 146/219 (66%), Positives = 188/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG +KD G+S+ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEVL
Sbjct: 304 QEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVL 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KP+F+R+ +LK VSMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG QVM+P
Sbjct: 364 QAFGKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPAGTQVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVMLHREKSIWGEDATEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF+F ++PSY HAPFT+LT+QPQ G+ V++ KL
Sbjct: 484 LAMILQRFTFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522


>gb|AGX93046.1| secologanin synthase-like protein [Amsonia hubrichtii]
          Length = 524

 Score =  330 bits (845), Expect = 1e-87
 Identities = 147/219 (67%), Positives = 188/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG +K+ G+++ DVI E KLF+FAGQETTG+LLTW  +LLS+HP WQERAREEVL
Sbjct: 304 QEIQKQGNKKNVGMTINDVIEECKLFYFAGQETTGVLLTWATILLSKHPEWQERAREEVL 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KPDF+R+ +LK VSMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG QVM+P
Sbjct: 364 QAFGKNKPDFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPAGTQVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVILHREKSIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF+F +SPSYAHAPFT+LTLQPQ G+ V++ KL
Sbjct: 484 LAMILQRFTFDVSPSYAHAPFTILTLQPQLGSHVIYKKL 522


>gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]
          Length = 516

 Score =  329 bits (844), Expect = 1e-87
 Identities = 146/219 (66%), Positives = 187/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG RKD G+++ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEVL
Sbjct: 293 QEIQKQGNRKDGGMTINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVL 352

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KP+F+R+ +LK VSMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG QVM+P
Sbjct: 353 QAFGKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIIHEDTKLGPYTIPAGTQVMLP 412

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 413 TVMLHREKSIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 472

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF F ++PSY HAPFT+LT+QPQ G+ V++ KL
Sbjct: 473 LAMILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 511


>gb|AGX93064.1| secologanin synthase [Catharanthus roseus]
           gi|589097610|gb|AHK60848.1| secologanin synthase-like
           protein [Catharanthus roseus]
          Length = 527

 Score =  329 bits (844), Expect = 1e-87
 Identities = 146/219 (66%), Positives = 187/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG RKD G+++ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEVL
Sbjct: 304 QEIQKQGNRKDGGMTINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVL 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KP+F+R+ +LK VSMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG QVM+P
Sbjct: 364 QAFGKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIIHEDTKLGPYTIPAGTQVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVMLHREKSIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF F ++PSY HAPFT+LT+QPQ G+ V++ KL
Sbjct: 484 LAMILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522


>gb|AGX93047.1| secologanin synthase-like protein [Rauvolfia serpentina]
          Length = 524

 Score =  328 bits (842), Expect = 2e-87
 Identities = 146/219 (66%), Positives = 187/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG +KD G+S+ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEV 
Sbjct: 304 QEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVF 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KP+F+R+ +LK +SMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG QVM+P
Sbjct: 364 QAFGKDKPEFERLNHLKYISMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPAGTQVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVMLHREKSIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF F ++PSYAHAP+TVLTLQPQ G+ V++ KL
Sbjct: 484 LAMILQRFKFDVAPSYAHAPYTVLTLQPQLGSHVIYKKL 522


>gb|AGX93048.1| secologanin synthase-like protein [Tabernaemontana elegans]
          Length = 524

 Score =  328 bits (840), Expect = 4e-87
 Identities = 148/219 (67%), Positives = 188/219 (85%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E E+QG +KD G+++ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQ+ AREEVL
Sbjct: 304 QEIEKQGNKKDVGMTIHDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQKLAREEVL 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           QVFG  KPDF+R+ +LK VSMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG  VM+P
Sbjct: 364 QVFGKKKPDFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPAGTLVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVMLHREKSIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF+F ++PSYAHAPFTVLTLQPQ G+ V++ KL
Sbjct: 484 LAMILQRFTFDVAPSYAHAPFTVLTLQPQLGSHVIYKKL 522


>dbj|BAP90521.1| secologanin synthase [Ophiorrhiza pumila]
          Length = 520

 Score =  327 bits (839), Expect = 5e-87
 Identities = 148/219 (67%), Positives = 186/219 (84%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E + QG  K+ GLS++DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQ+RAREEVL
Sbjct: 301 QEIKDQGNEKNVGLSIDDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQDRAREEVL 360

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           Q FG  KP+FD++  LK VSMILYEVLRLYPPVI+LTK+ HK+TKLG   IPAG QVMMP
Sbjct: 361 QTFGKNKPEFDKLNQLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGPYIIPAGTQVMMP 420

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHR+  +WG+DA EFNP RFA+G++NATK+   ++PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 421 TVMLHREKSIWGEDAMEFNPERFADGVANATKNNVSFLPFSWGPRVCLGQNFALLQAKLG 480

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF+F +SPSYAHAP+TVLTLQPQ G+ V++ KL
Sbjct: 481 LAMILQRFTFDVSPSYAHAPYTVLTLQPQLGSHVIYSKL 519


>gb|AKF02530.1| secologanin synthase-like protein 4 [Catharanthus roseus]
          Length = 524

 Score =  325 bits (832), Expect = 3e-86
 Identities = 144/219 (65%), Positives = 186/219 (84%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG +KD G+S+ DVI E KLF+FAGQETTG+LLTWT +LLS+HP WQERAREEVL
Sbjct: 304 KEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVL 363

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           + FG  KP+ + + +LK VSMILYEVLRLYPPVI+LTK+ H++TKLG  TIPAG QVM+P
Sbjct: 364 EAFGKNKPELECLNHLKYVSMILYEVLRLYPPVIDLTKIVHEDTKLGPYTIPAGTQVMLP 423

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           +++LHRD ++WG+DA EFNP RFA+G++NATK+   Y+PFSWGPR+C+GQNFALLQAK+ 
Sbjct: 424 TVMLHRDKNIWGEDAMEFNPMRFADGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLG 483

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           L +ILQRF F ++PSY HAPFT+LT+QPQ G+ V++ KL
Sbjct: 484 LAMILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522


>gb|AGX93045.1| secologanin synthase-like protein, partial [Cinchona calisaya]
          Length = 518

 Score =  317 bits (813), Expect = 5e-84
 Identities = 140/219 (63%), Positives = 183/219 (83%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E ++QG +K+ G++++DVI E KLF+FAGQETTG+LLTWT ++LS+HP WQ+RAREEVL
Sbjct: 300 KEIQKQGNKKNVGMTIDDVIEECKLFYFAGQETTGVLLTWTMIMLSKHPEWQDRAREEVL 359

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
             FG   P+FD++ +LK VSMILYEVLRLYPPV +L KV +++TKLG  TIPAG Q+ +P
Sbjct: 360 HAFGKNTPEFDKLNHLKYVSMILYEVLRLYPPVFDLNKVVYEDTKLGTYTIPAGTQIELP 419

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
           S+++HRD  +WG+DA EFNP RFA G++ ATK+   YIPFSWGPR+C+GQNFA+LQAK+ 
Sbjct: 420 SVMIHRDKTIWGEDAMEFNPMRFANGVAAATKNQVAYIPFSWGPRVCLGQNFAILQAKLG 479

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
           LT+ILQRFSF +SPSY HAPFT+L+LQPQ G+ V+F KL
Sbjct: 480 LTMILQRFSFDVSPSYVHAPFTILSLQPQFGSHVIFRKL 518


>ref|XP_011009102.1| PREDICTED: cytochrome P450 72A15-like isoform X1 [Populus
           euphratica] gi|743929736|ref|XP_011009103.1| PREDICTED:
           cytochrome P450 72A15-like isoform X2 [Populus
           euphratica]
          Length = 542

 Score =  314 bits (804), Expect = 6e-83
 Identities = 144/218 (66%), Positives = 181/218 (83%)
 Frame = -2

Query: 834 ERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQ 655
           E +  G  K AG+S+EDVI E K+F+FAGQETT +LLTWT VLL+++P+WQ RAREEV+Q
Sbjct: 325 EIQEHGNSKSAGMSIEDVIDECKIFYFAGQETTSVLLTWTMVLLAQYPNWQARAREEVVQ 384

Query: 654 VFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPS 475
           VFGN KPDFD + +LK+V+MILYEVLRLYPPVI L +  H+E KLGNL +PAGVQ+ +P+
Sbjct: 385 VFGNKKPDFDGLNHLKVVTMILYEVLRLYPPVIMLNRDVHEEIKLGNLLVPAGVQISVPT 444

Query: 474 ILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMAL 295
           ILLH+D ++WGDDA EF P RFAEG+S ATKS   ++PF WGPR+CVGQNFA+++AKMAL
Sbjct: 445 ILLHQDRELWGDDASEFKPERFAEGVSKATKSQVSFLPFGWGPRICVGQNFAMIEAKMAL 504

Query: 294 TLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
            +ILQR SF++SPSY HAP TV+TLQPQHGA ++  KL
Sbjct: 505 AMILQRCSFELSPSYIHAPRTVITLQPQHGAPMILRKL 542


>ref|XP_006377681.1| cytochrome P450 family protein [Populus trichocarpa]
           gi|550328061|gb|ERP55478.1| cytochrome P450 family
           protein [Populus trichocarpa]
          Length = 410

 Score =  313 bits (803), Expect = 8e-83
 Identities = 143/218 (65%), Positives = 181/218 (83%)
 Frame = -2

Query: 834 ERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQ 655
           E +  G  K  G+S+EDVI E K+F+FAGQETT +LLTWT +LL+++P+WQ RAREEV+Q
Sbjct: 193 EIQEHGNTKSVGMSIEDVIDECKIFYFAGQETTLVLLTWTMILLAQYPNWQARAREEVVQ 252

Query: 654 VFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPS 475
           VFGN KPDFD + +LK+V+MILYEVLRLYPPVI L +  H+E KLGNL +PAGVQV +P+
Sbjct: 253 VFGNKKPDFDGLNHLKVVTMILYEVLRLYPPVITLNRDVHEEIKLGNLLLPAGVQVSLPT 312

Query: 474 ILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMAL 295
           ILLH+D ++WGDDA EF P RFAEG+S ATKS   ++PF WGPR+CVGQNFAL++AKMAL
Sbjct: 313 ILLHQDHELWGDDASEFKPERFAEGVSKATKSQVSFLPFGWGPRICVGQNFALIEAKMAL 372

Query: 294 TLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
            ++LQR+SF++SPSY HAP TV+TLQPQHGA ++  KL
Sbjct: 373 AMVLQRYSFELSPSYIHAPRTVITLQPQHGAPMILRKL 410


>ref|XP_006377693.1| hypothetical protein POPTR_0011s10300g [Populus trichocarpa]
           gi|550328073|gb|ERP55490.1| hypothetical protein
           POPTR_0011s10300g [Populus trichocarpa]
          Length = 518

 Score =  313 bits (801), Expect = 1e-82
 Identities = 144/218 (66%), Positives = 180/218 (82%)
 Frame = -2

Query: 834 ERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQ 655
           E +  G  K  G+S+EDVI E ++F+FAGQETT +LLTWT VLL+++P+WQ RAREEV+Q
Sbjct: 301 EIQEHGNTKSVGMSIEDVIDECRIFYFAGQETTTVLLTWTMVLLAQYPNWQARAREEVVQ 360

Query: 654 VFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPS 475
           VFGN KPDFD + +LK+V+MI YEVLRLYPPVI L +  H+E KLGNL +PAGVQV +P+
Sbjct: 361 VFGNKKPDFDGLNHLKVVTMIFYEVLRLYPPVIMLNRDVHEEIKLGNLLLPAGVQVSVPT 420

Query: 474 ILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMAL 295
           ILLH+D ++WGDDA EF PGRFAEG+S ATKS   ++PF WGPR+CVGQNFAL++AKMAL
Sbjct: 421 ILLHQDHELWGDDASEFKPGRFAEGVSKATKSQVSFLPFGWGPRICVGQNFALIEAKMAL 480

Query: 294 TLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
            +ILQR SF++SPSY HAP TV+TLQPQHGA ++  KL
Sbjct: 481 AMILQRCSFELSPSYIHAPRTVITLQPQHGAPMILRKL 518


>ref|XP_011009101.1| PREDICTED: cytochrome P450 CYP72A219-like [Populus euphratica]
          Length = 547

 Score =  311 bits (798), Expect = 3e-82
 Identities = 142/218 (65%), Positives = 179/218 (82%)
 Frame = -2

Query: 834 ERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQ 655
           E +  G  K  G+S+EDVI E K+F+FAGQETT +LLTWT VLL+++P+WQ R REEV+Q
Sbjct: 330 EIQEHGNTKSVGMSIEDVIDECKIFYFAGQETTSVLLTWTMVLLAQYPNWQARGREEVVQ 389

Query: 654 VFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPS 475
           VFGN KPDFD + +LK+V+MILYEVLRLYPPVI L +  H+E KLGNL +PAGVQ+ +P+
Sbjct: 390 VFGNKKPDFDGLNHLKVVTMILYEVLRLYPPVIMLNRDVHEEIKLGNLLLPAGVQISVPT 449

Query: 474 ILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMAL 295
           ILLH+D ++WGDDA EF P RFAEG+S ATKS   ++PF WGPR+CVGQNFA+++AKMAL
Sbjct: 450 ILLHQDRELWGDDASEFKPERFAEGVSKATKSQVSFLPFGWGPRICVGQNFAMIEAKMAL 509

Query: 294 TLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
            +ILQR SF++SPSY HAP TV+TLQPQHGA ++  KL
Sbjct: 510 AMILQRCSFELSPSYVHAPHTVITLQPQHGAPMILRKL 547


>gb|AGX93056.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Lonicera japonica]
          Length = 519

 Score =  311 bits (796), Expect = 5e-82
 Identities = 145/214 (67%), Positives = 178/214 (83%)
 Frame = -2

Query: 822 QGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQVFGN 643
           Q   K+AGLS+E++I E KLF+FAGQ+TT  LL WT V+LSR P WQ RAREEVLQVFG+
Sbjct: 306 QTKNKNAGLSIEEIIGECKLFYFAGQDTTSTLLCWTMVILSRFPDWQARAREEVLQVFGD 365

Query: 642 GKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPSILLH 463
           GKPD+D I  LK V+MILYEVLRLYPPV+ELTKV H++TKLG+LTIPAGVQVM+P+ILLH
Sbjct: 366 GKPDYDGINRLKTVTMILYEVLRLYPPVVELTKVAHEDTKLGDLTIPAGVQVMLPTILLH 425

Query: 462 RDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMALTLIL 283
            + D+WG+D  EF P RFA+G+  ATKS   + PFS GPRMC+GQNFALL+AKMAL LIL
Sbjct: 426 HNPDIWGEDVDEFKPERFAQGVLKATKSQGSFFPFSLGPRMCIGQNFALLEAKMALALIL 485

Query: 282 QRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
            RFSF++SPSY HAP+T++T+QPQ GA ++ HK+
Sbjct: 486 PRFSFELSPSYVHAPYTLITMQPQFGAHLILHKI 519


>ref|XP_006377690.1| cytochrome P450 family protein [Populus trichocarpa]
           gi|550328070|gb|ERP55487.1| cytochrome P450 family
           protein [Populus trichocarpa]
          Length = 353

 Score =  310 bits (795), Expect = 6e-82
 Identities = 145/218 (66%), Positives = 179/218 (82%)
 Frame = -2

Query: 834 ERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVLQ 655
           E +  G  K  G+S+EDVI E KLF+FAGQETT +LLTWT VLL+++P+WQ RAREEV+Q
Sbjct: 136 EIQEHGNTKSVGMSIEDVIDECKLFYFAGQETTLVLLTWTMVLLAQYPNWQARAREEVVQ 195

Query: 654 VFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMPS 475
           VFGN KPDFD + +LK+V+MILYEVLRLYPPVI L +  H+E KLGNL +PAGVQV +P 
Sbjct: 196 VFGNKKPDFDGLNHLKVVTMILYEVLRLYPPVIMLNRDVHEEIKLGNLLLPAGVQVSLPI 255

Query: 474 ILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMAL 295
           ILLH+D ++WGDDA EF P RFAEGIS ATKS   ++PF WGPR+CVGQNFAL++AKMAL
Sbjct: 256 ILLHQDHELWGDDASEFKPERFAEGISKATKSQVSFLPFGWGPRICVGQNFALIEAKMAL 315

Query: 294 TLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHKL 181
            ++LQR+SF++S SY HAP TV+TLQPQHGA ++  KL
Sbjct: 316 AMVLQRYSFELSSSYIHAPRTVITLQPQHGAPMILRKL 353


>ref|XP_010092744.1| Secologanin synthase [Morus notabilis] gi|587862278|gb|EXB52089.1|
           Secologanin synthase [Morus notabilis]
          Length = 558

 Score =  308 bits (789), Expect = 3e-81
 Identities = 141/218 (64%), Positives = 177/218 (81%)
 Frame = -2

Query: 837 EERERQGYRKDAGLSLEDVISESKLFFFAGQETTGILLTWTCVLLSRHPHWQERAREEVL 658
           +E +  G  K+ G+S+ DVI E KLF+FAGQETT +LL WT VLLSR+P WQ RAREEVL
Sbjct: 340 KEIQEHGNNKNMGISIRDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVL 399

Query: 657 QVFGNGKPDFDRIQNLKIVSMILYEVLRLYPPVIELTKVTHKETKLGNLTIPAGVQVMMP 478
           QVFG   PDFD + +LKIV+MILYEVLR YPPVI LT+   K+T+LGNL++P GVQ+ +P
Sbjct: 400 QVFGYNNPDFDGLGHLKIVTMILYEVLRFYPPVILLTRTVQKQTQLGNLSLPPGVQISLP 459

Query: 477 SILLHRDSDMWGDDAKEFNPGRFAEGISNATKSPYFYIPFSWGPRMCVGQNFALLQAKMA 298
            IL+HRD+++WG+DAKEF P RFAEG+S ATK    + PF WGPR+C+GQNFA+++AKMA
Sbjct: 460 VILVHRDTNLWGEDAKEFKPERFAEGVSKATKGQVSFFPFGWGPRICIGQNFAMIEAKMA 519

Query: 297 LTLILQRFSFQISPSYAHAPFTVLTLQPQHGAQVVFHK 184
           L LILQRF+F++SPSYAHAP TV+TLQPQ+GA +  HK
Sbjct: 520 LALILQRFTFELSPSYAHAPCTVITLQPQYGAHITLHK 557