BLASTX nr result
ID: Coptis25_contig00030858
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00030858
(625 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop... 288 4e-76
emb|CBI24150.3| unnamed protein product [Vitis vinifera] 288 4e-76
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis] 285 6e-75
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso... 283 1e-74
gb|AFG18184.1| ent-kaurene synthase [Malus x domestica] 281 5e-74
>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
vinifera]
Length = 793
Score = 288 bits (738), Expect = 4e-76
Identities = 137/208 (65%), Positives = 167/208 (80%)
Frame = -2
Query: 624 ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 445
AT+F PE SDAR+SWAK VLTTVVDDF+D+GGS+EEL NLI L+EKWD A D CSEQ
Sbjct: 517 ATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQ 576
Query: 444 VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 265
VEIV++AL STI E+G KA WQ R+VT HI++IWL L++SM++E++W NKS PTMDEY
Sbjct: 577 VEIVFSALHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEY 636
Query: 264 NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 85
NAY SFALGPIVLPALYFVGP+LSEE V ECH L++LMSTCGR LNDI FKRE+
Sbjct: 637 MTNAYVSFALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLMSTCGRLLNDIHSFKRESK 696
Query: 84 EGKLNGVSLLMIHDPTVSNEEEAMKKMR 1
EGK N ++L MIH V+ EE+A+++M+
Sbjct: 697 EGKANALALHMIHGNGVTTEEQAIREMK 724
>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
Length = 826
Score = 288 bits (738), Expect = 4e-76
Identities = 137/208 (65%), Positives = 167/208 (80%)
Frame = -2
Query: 624 ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 445
AT+F PE SDAR+SWAK VLTTVVDDF+D+GGS+EEL NLI L+EKWD A D CSEQ
Sbjct: 550 ATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQ 609
Query: 444 VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 265
VEIV++AL STI E+G KA WQ R+VT HI++IWL L++SM++E++W NKS PTMDEY
Sbjct: 610 VEIVFSALHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEY 669
Query: 264 NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 85
NAY SFALGPIVLPALYFVGP+LSEE V ECH L++LMSTCGR LNDI FKRE+
Sbjct: 670 MTNAYVSFALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLMSTCGRLLNDIHSFKRESK 729
Query: 84 EGKLNGVSLLMIHDPTVSNEEEAMKKMR 1
EGK N ++L MIH V+ EE+A+++M+
Sbjct: 730 EGKANALALHMIHGNGVTTEEQAIREMK 757
>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
Length = 737
Score = 285 bits (728), Expect = 6e-75
Identities = 145/208 (69%), Positives = 168/208 (80%)
Frame = -2
Query: 624 ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 445
ATLF PELSDAR+SWAK GVLTTVVDDF+DVGGS+EEL NLI L+EKWD D CSE
Sbjct: 462 ATLFPPELSDARISWAKNGVLTTVVDDFFDVGGSEEELINLIQLVEKWDVNVGVDCCSEN 521
Query: 444 VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 265
VEI+++AL++TI E+G +AF Q R VT H++EIWL LIKSM KE+EW NKSVPTM+EY
Sbjct: 522 VEIIFSALKNTINEIGARAFTRQGRSVTSHVIEIWLDLIKSMFKEAEWLRNKSVPTMEEY 581
Query: 264 NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 85
NAY SFALGPIVLPALY VGP+LSEEAV S E +L+RLMST GR LNDIQGFKRE+
Sbjct: 582 MENAYVSFALGPIVLPALYLVGPKLSEEAVGSSEFDHLYRLMSTAGRLLNDIQGFKRESA 641
Query: 84 EGKLNGVSLLMIHDPTVSNEEEAMKKMR 1
EGKLN VSL MIH V+ EEEA+ +M+
Sbjct: 642 EGKLNAVSLAMIHGNGVT-EEEAINEMK 668
>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
synthase B, chloroplast precursor, putative [Ricinus
communis]
Length = 808
Score = 283 bits (725), Expect = 1e-74
Identities = 137/208 (65%), Positives = 168/208 (80%)
Frame = -2
Query: 624 ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 445
ATLF PELSDAR+SWAK GVLTTVVDDF+DVGGS EEL NLI L+EKWD + FCSEQ
Sbjct: 462 ATLFAPELSDARLSWAKNGVLTTVVDDFFDVGGSVEELINLIQLIEKWDVDESTHFCSEQ 521
Query: 444 VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 265
VEI+++ALRSTI E+G+KAF WQ R VT H+++IWL L+KSM+ E+ WT++KS+PT+DEY
Sbjct: 522 VEIIFSALRSTISEIGDKAFTWQGRKVTSHVIKIWLDLLKSMLTETLWTKSKSIPTLDEY 581
Query: 264 NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 85
N Y SFALGPIVLPAL+ VGP+L+EE VR E H+LF+ M TCGR LND +GF+RE+
Sbjct: 582 MINGYVSFALGPIVLPALFLVGPKLTEEDVRDPELHDLFKAMGTCGRLLNDWRGFQRESK 641
Query: 84 EGKLNGVSLLMIHDPTVSNEEEAMKKMR 1
EGKLN VSL MI NEEEA++K++
Sbjct: 642 EGKLNAVSLHMIQGNGGVNEEEAIRKIK 669
>gb|AFG18184.1| ent-kaurene synthase [Malus x domestica]
Length = 737
Score = 281 bits (720), Expect = 5e-74
Identities = 143/208 (68%), Positives = 169/208 (81%)
Frame = -2
Query: 624 ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 445
ATLF PELSDAR+SWAK GVLTTVVDDF+DVGGS+EEL NLI L+EKWD + D CSE
Sbjct: 462 ATLFPPELSDARISWAKNGVLTTVVDDFFDVGGSEEELINLIQLVEKWDVNVSVDCCSEN 521
Query: 444 VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 265
VEI+++AL++TI E+G +AF Q R VT H++EIWL LIKSM KE++W +NKSVPTM+EY
Sbjct: 522 VEIIFSALKNTINEIGARAFTRQGRSVTSHVIEIWLDLIKSMFKEAQWLKNKSVPTMEEY 581
Query: 264 NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 85
NAY SFALGPIVLPALY VGP+LSEEAV S E +L+RLMST GR LN IQGFKRE+
Sbjct: 582 MENAYVSFALGPIVLPALYLVGPKLSEEAVGSSEFDHLYRLMSTAGRLLNGIQGFKRESA 641
Query: 84 EGKLNGVSLLMIHDPTVSNEEEAMKKMR 1
EGKLN VSL MIH V+ EEEA+ +M+
Sbjct: 642 EGKLNAVSLAMIHGNGVT-EEEAINEMK 668