BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00030393
         (977 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003632058.1| PREDICTED: probable pectinesterase/pectinest...   192   1e-46
ref|XP_002527752.1| Pectinesterase-2 precursor, putative [Ricinu...   164   4e-38
ref|NP_191460.1| pectinesterase 35 [Arabidopsis thaliana] gi|753...   157   3e-36
ref|XP_002878254.1| pectinesterase family protein [Arabidopsis l...   147   5e-33
ref|NP_001148248.1| pectinesterase-2 [Zea mays] gi|195616932|gb|...   145   2e-32

>ref|XP_003632058.1| PREDICTED: probable pectinesterase/pectinesterase inhibitor 35-like
           [Vitis vinifera]
          Length = 553

 Score =  192 bits (488), Expect = 1e-46
 Identities = 115/275 (41%), Positives = 165/275 (60%), Gaps = 4/275 (1%)
 Frame = +3

Query: 165 SSVHISTVSKXXXXXXXPFPCKDTLYPKACESQILSAKDASSTPITLDDVLNLSIKSVID 344
           SS H STV K          CK T Y  AC+S +LS+ + +  P T  D+ +LS++  ++
Sbjct: 39  SSSHTSTVPKASMEAIVSAACKATFYQTACQSALLSSTNGA-VPQTQADLFDLSVQFSLN 97

Query: 345 RATLAKSLAQNVSLMNQRENPNSFTALDDCVELLDHSIDQLNSILXXXXXXXXXXXXXX- 521
           +A  A++   ++ L++ +      T  DDC+ELLD ++DQL ++                
Sbjct: 98  QARSARAHVHDLRLLDHKTQIVRGT--DDCMELLDDTLDQLTNVANRRKTLIEDPDDVQT 155

Query: 522 WLSTAITHQTTCLDGLQTHKFFKEKELIHATLQSNIQHVSNSLVIHKSIVSAKTL-KVST 698
           WLS A+T+Q TCL+ +QT++   +  L+    Q+    +SNSL +H S   +K   + +T
Sbjct: 156 WLSAALTNQATCLESIQTYQTGGQNGLMRPMAQNLTYSISNSLALHMSTRPSKEAQRTNT 215

Query: 699 AG--RRLLSDDFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEALAFASLVGG 872
           AG  RRLLSD FP WV+  ERKLLE+S+++I   AVVA DG+GTH+TI EALA    + G
Sbjct: 216 AGHHRRLLSDRFPGWVTAAERKLLEASVEEIGATAVVAKDGSGTHKTIGEALAMVVTLEG 275

Query: 873 GGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKG 977
            GRTVIHV AGTY+E  + + + Q NVMLVGDGKG
Sbjct: 276 EGRTVIHVKAGTYDE-GLKIPSSQKNVMLVGDGKG 309


>ref|XP_002527752.1| Pectinesterase-2 precursor, putative [Ricinus communis]
           gi|223532839|gb|EEF34613.1| Pectinesterase-2 precursor,
           putative [Ricinus communis]
          Length = 583

 Score =  164 bits (414), Expect = 4e-38
 Identities = 114/271 (42%), Positives = 156/271 (57%), Gaps = 20/271 (7%)
 Frame = +3

Query: 225 CKDTLYPKACESQILSAKDASSTPITLDDVLNLSIKSVIDRATLAKSLAQNVSLMNQ-RE 401
           C D  Y  ACES + S K  ++ P T  ++ + S+   + +A  A  LA  VS  N  + 
Sbjct: 71  CNDIPYKSACESVLSSLK--TTIPTTPKELFDHSVHYTLKQARSAFELASTVSPSNNVKA 128

Query: 402 NPNSFTA--------LDDCVELLDHSIDQLNSILXXXXXXXXXXXXXX-WLSTAITHQTT 554
             N+  A        + DCVELL+ S+D L++I                WLS A+T+Q T
Sbjct: 129 AANTHLAAVAGGGGGMIDCVELLEDSLDLLSTITNRKNNPKHTHNDVQTWLSAALTNQET 188

Query: 555 CLDGLQTHKFF----KEKELIHATLQSNIQHVSNSLVIHKSIVSAKTLKVSTAGRRLLS- 719
           C++ LQ  K      + +E+I  T ++  QH+SNSL ++ S     T + +  GR+LLS 
Sbjct: 189 CIESLQNDKLLFKLDRGQEMIR-TARNLSQHISNSLALYMSHYY-NTKESNKGGRKLLSG 246

Query: 720 DDFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEALAFA---SLV--GGGGRT 884
           DDFP WVS  ERKLLE+ + +I+ +AVVA DG+GTH TI EA+A     SL   GGGGR 
Sbjct: 247 DDFPAWVSESERKLLETPVAEIKAHAVVAKDGSGTHTTIGEAIAEVMSYSLAGEGGGGRN 306

Query: 885 VIHVTAGTYNENNIMVSAKQSNVMLVGDGKG 977
           VI+V AGTY+E N+ +  KQ NVMLVGDGKG
Sbjct: 307 VIYVKAGTYHE-NLKLPTKQKNVMLVGDGKG 336


>ref|NP_191460.1| pectinesterase 35 [Arabidopsis thaliana]
           gi|75311708|sp|Q9LYT5.1|PME35_ARATH RecName:
           Full=Probable pectinesterase/pectinesterase inhibitor
           35; Includes: RecName: Full=Pectinesterase inhibitor 35;
           AltName: Full=Pectin methylesterase inhibitor 35;
           Includes: RecName: Full=Pectinesterase 35; Short=PE 35;
           AltName: Full=Pectin methylesterase 35; Short=AtPME35;
           Flags: Precursor gi|7529744|emb|CAB86929.1|
           pectinesterase precursor-like protein [Arabidopsis
           thaliana] gi|16974625|gb|AAL31215.1| AT3g59010/F17J16_60
           [Arabidopsis thaliana] gi|24111417|gb|AAN46858.1|
           At3g59010/F17J16_60 [Arabidopsis thaliana]
           gi|332646339|gb|AEE79860.1| pectinesterase 35
           [Arabidopsis thaliana]
          Length = 529

 Score =  157 bits (398), Expect = 3e-36
 Identities = 93/221 (42%), Positives = 137/221 (61%), Gaps = 3/221 (1%)
 Frame = +3

Query: 324 SIKSVIDRATLAKSLAQNVSLMNQRENPNSFTALDDCVELLDHSIDQLNSILXXXXXXXX 503
           S++  +D A  A+SLA N++L ++         ++DC+ELLD ++D L  I+        
Sbjct: 69  SVQLSLDHALFARSLAFNLTLSHRTSQTLMLDPVNDCLELLDDTLDMLYRIVVIKRKDHV 128

Query: 504 XXXXXXWLSTAITHQTTCLDGLQTHKFFKEKELIHATLQSNIQHV-SNSLVIHKSIVSAK 680
                 WLS A+T+Q TC   L     F ++ +   +   N+  + +NSL +  S     
Sbjct: 129 NDDVHTWLSAALTNQETCKQSLSEKSSFNKEGIAIDSFARNLTGLLTNSLDMFVSDKQKS 188

Query: 681 TLKVS-TAGRRLLSD-DFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEALAF 854
           +   + T GR+LLSD DFPTWVS+ +RKLLE+S++++  +AVVA DG+GTH +++EAL  
Sbjct: 189 SSSSNLTGGRKLLSDHDFPTWVSSSDRKLLEASVEELRPHAVVAADGSGTHMSVAEAL-- 246

Query: 855 ASLVGGGGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKG 977
           ASL  G GR+VIH+TAGTY E N+ + +KQ NVMLVGDGKG
Sbjct: 247 ASLEKGSGRSVIHLTAGTYKE-NLNIPSKQKNVMLVGDGKG 286


>ref|XP_002878254.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
           gi|297324092|gb|EFH54513.1| pectinesterase family
           protein [Arabidopsis lyrata subsp. lyrata]
          Length = 525

 Score =  147 bits (370), Expect = 5e-33
 Identities = 91/224 (40%), Positives = 134/224 (59%), Gaps = 6/224 (2%)
 Frame = +3

Query: 324 SIKSVIDRATLAKSLAQNVSLMNQRENPNSFTALDDCVELLDHSIDQLNSILXXXXXXXX 503
           S++  ++ A  A+S A N++L ++         ++DC+ELLD ++D L+ I+        
Sbjct: 65  SVQLSLEDALFARSFAFNLTLSHRTSQSLMLDPVNDCLELLDDTLDMLSRIVVIKRKDHV 124

Query: 504 XXXXXXWLSTAITHQTTCLDGLQTHKFFKEKELIHATLQSNIQHV-SNSLVIHKSIVSAK 680
                 WLS A+T+Q TC   L     F +  +   +   N+  + +NSL +    VS K
Sbjct: 125 NDDVHTWLSAALTNQETCKQSLSEKSSFNKDGIAIDSFARNLTGLLTNSLDM---FVSDK 181

Query: 681 TLKVS----TAGRRLLSD-DFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEA 845
               S    T GR+LLS+ DFP+W S  +RKLLE+S++++  +AVVA DG+GTH +I+EA
Sbjct: 182 RKSSSSSRLTGGRKLLSEHDFPSWFSMSDRKLLEASVEELRPHAVVAADGSGTHMSIAEA 241

Query: 846 LAFASLVGGGGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKG 977
           L  ASL  G GR+VIH+ AGTY E N+ + +KQ NVMLVGDGKG
Sbjct: 242 L--ASLEKGSGRSVIHLAAGTYKE-NLNIPSKQKNVMLVGDGKG 282


>ref|NP_001148248.1| pectinesterase-2 [Zea mays] gi|195616932|gb|ACG30296.1|
           pectinesterase-2 precursor [Zea mays]
           gi|414591337|tpg|DAA41908.1| TPA: pectinesterase [Zea
           mays]
          Length = 578

 Score =  145 bits (365), Expect = 2e-32
 Identities = 100/261 (38%), Positives = 137/261 (52%), Gaps = 10/261 (3%)
 Frame = +3

Query: 225 CKDTLYPKACESQILSAKDASSTPITLDDVLNLSIKSVIDRATLAKSLAQNVSLMNQREN 404
           C  T YP ACE+ + S    SS     DD    S+   + R   A+++A+N+S  + R  
Sbjct: 77  CLSTPYPSACETALSSPAQGSSGT---DDPFATSVHYAMARVASARAVARNLSAAHLR-G 132

Query: 405 PNSFTALDDCVELLDHSIDQLNSILXXXXXXXXXXXXXXWLSTAITHQTTCLDGLQTHKF 584
                 + DC ELLD S+DQL   L              WLS A+T+Q TC D L     
Sbjct: 133 ARPPPGVQDCAELLDISLDQLGDALAAAARDADGVTT--WLSAALTNQATCDDSLAADPD 190

Query: 585 FKEKELIHATLQSNIQHVSNSLVIHKSIVSAKTL---KVSTAGRRLLSDDFPTWVSTGER 755
              +  I A L +  Q ++ +L +H +   A        S+      +  FP+WV+  +R
Sbjct: 191 SAGRGAIRARLSALTQFIATALALHVNKSKAHHSGGGSPSSGSLPTPASPFPSWVTQQDR 250

Query: 756 KLLESSIKD----IEVNAVVAMDGTGTHRTISEALAFASLV---GGGGRTVIHVTAGTYN 914
           KLLESS       + V+AVVA+DG+GTHRTI+EA+A  +     GGG R VIHV AG Y 
Sbjct: 251 KLLESSHAGASGGLAVDAVVALDGSGTHRTINEAIAAVTTTAANGGGARKVIHVKAGRY- 309

Query: 915 ENNIMVSAKQSNVMLVGDGKG 977
           E ++ VS+KQ NVML+GDGKG
Sbjct: 310 EESVTVSSKQKNVMLMGDGKG 330