BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00030165
         (804 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI15698.3| unnamed protein product [Vitis vinifera]              217   3e-54
gb|ABM30178.1| steroleosin [Brassica napus]                           214   1e-53
gb|ACG69523.1| steroleosin SLO1-2 [Brassica napus]                    213   4e-53
gb|ABM90633.1| short-chain dehydrogenase reductase [Brassica nap...   212   9e-53
gb|ACG69524.1| steroleosin SLO1-3 [Brassica napus]                    209   4e-52

>emb|CBI15698.3| unnamed protein product [Vitis vinifera]
          Length = 322

 Score =  217 bits (552), Expect = 3e-54
 Identities = 103/204 (50%), Positives = 144/204 (70%), Gaps = 1/204 (0%)
 Frame = +2

Query: 20  VDHMVNNAGIISAFYFEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASV 199
           VDH+VNNAGI SA   EE+ D+T+  PVMDVNFWGSVY T  A+PHL+ S GKI+ +AS 
Sbjct: 100 VDHLVNNAGIQSASTLEEATDITNFRPVMDVNFWGSVYTTRFAIPHLRNSHGKIIGMASS 159

Query: 200 SGWLYGPNISFYAASKAALLNFFDTLRVEFGSSV-KVTIASPGFVESEMSQGKHRTMQGK 376
           + WL  P +S Y ASKAAL++F+DTLR+E GS +  +T+ +PGF+ESEM+QG+    + +
Sbjct: 160 AAWLPMPRMSIYNASKAALISFYDTLRIELGSDIGGITVVTPGFIESEMTQGEFLKNEDQ 219

Query: 377 VEIDKEKRDALIGNFPVRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEW 556
           +E+D++ RDA +   PV  A  CAK I+K   RGE+++T+PSWFR+ Y+ KVL PEV+EW
Sbjct: 220 IEVDQDMRDAQVSIIPVGRAEACAKAIVKSACRGEKYLTEPSWFRVTYLWKVLCPEVIEW 279

Query: 557 FFHVLFVILPNRNKDKSSLQKSIS 628
            F + ++  P  ++  S     IS
Sbjct: 280 CFRLFYLARPGESETLSKKILDIS 303


>gb|ABM30178.1| steroleosin [Brassica napus]
          Length = 244

 Score =  214 bits (546), Expect = 1e-53
 Identities = 102/211 (48%), Positives = 151/211 (71%)
 Frame = +2

Query: 20  VDHMVNNAGIISAFYFEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASV 199
           +DH+VNNAGI+    FE   ++T +  VMD NFWGSVY T  ALP+L++S GKI+ ++S 
Sbjct: 21  LDHLVNNAGIMQISMFENIEEITRTRAVMDTNFWGSVYTTRAALPYLRQSNGKIVAMSSS 80

Query: 200 SGWLYGPNISFYAASKAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKV 379
           + WL  P +SFY ASKAALLNFF+TLR+E GS V +TI +PG++ESE++QGK+ + +G++
Sbjct: 81  AAWLTAPRMSFYNASKAALLNFFETLRIELGSDVHITIVTPGYIESELTQGKYFSGEGEL 140

Query: 380 EIDKEKRDALIGNFPVRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWF 559
            ++++ RD  IG FPV S   CAK I+KGV R +R+VT+PSWF++ Y+ KV  PE++EW 
Sbjct: 141 VVNQDIRDVQIGAFPVTSVSGCAKGIVKGVCRKQRYVTEPSWFKVTYLWKVFCPELIEWG 200

Query: 560 FHVLFVILPNRNKDKSSLQKSISNRNEIKKA 652
             +LF +  +   +K++L K I +   ++ A
Sbjct: 201 CRLLF-LSGHGTSEKNALNKKILDIPGVRSA 230


>gb|ACG69523.1| steroleosin SLO1-2 [Brassica napus]
          Length = 349

 Score =  213 bits (542), Expect = 4e-53
 Identities = 101/211 (47%), Positives = 151/211 (71%)
 Frame = +2

Query: 20  VDHMVNNAGIISAFYFEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASV 199
           +DH+VNNAGI+    FE   ++T +  VMD NFWGSVY T  ALP+L++S GKI+ ++S 
Sbjct: 126 LDHLVNNAGIMQISMFENIEEITRTRAVMDTNFWGSVYTTRAALPYLRQSNGKIVAMSSS 185

Query: 200 SGWLYGPNISFYAASKAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKV 379
           + WL  P +SFY ASKAALLNFF+TLR+E GS V +TI +PG++ESE++QGK+ + +G++
Sbjct: 186 AAWLTAPRMSFYNASKAALLNFFETLRIELGSDVHITIVTPGYIESELTQGKYFSGEGEL 245

Query: 380 EIDKEKRDALIGNFPVRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWF 559
            ++++ RD  IG FPV S   CAK I+KGV R +R+VT+PSWF++ Y+ KV  PE++EW 
Sbjct: 246 VVNQDIRDVQIGAFPVTSVSGCAKGIVKGVCRKQRYVTEPSWFKVTYLWKVFCPELIEWG 305

Query: 560 FHVLFVILPNRNKDKSSLQKSISNRNEIKKA 652
             +LF +  +   ++++L K I +   ++ A
Sbjct: 306 CRLLF-LSGHGTSEENALNKKILDIPGVRSA 335


>gb|ABM90633.1| short-chain dehydrogenase reductase [Brassica napus]
           gi|321173854|gb|ADW77633.1| steroleosin-A [Brassica
           napus] gi|321173856|gb|ADW77634.1| steroleosin-A
           [Brassica napus]
          Length = 349

 Score =  212 bits (539), Expect = 9e-53
 Identities = 100/211 (47%), Positives = 151/211 (71%)
 Frame = +2

Query: 20  VDHMVNNAGIISAFYFEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASV 199
           +DH+VNNAGI+    FE   ++T +  VMD NFWG+VY T  ALP+L++S GKI+ ++S 
Sbjct: 126 LDHLVNNAGIMQISMFENIEEITRTRAVMDTNFWGAVYTTRAALPYLRQSNGKIVAMSSS 185

Query: 200 SGWLYGPNISFYAASKAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKV 379
           + WL  P +SFY ASKAALLNFF+TLR+E GS V +TI +PG++ESE++QGK+ + +G++
Sbjct: 186 AAWLTAPRMSFYNASKAALLNFFETLRIELGSDVHITIVTPGYIESELTQGKYVSGEGEL 245

Query: 380 EIDKEKRDALIGNFPVRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWF 559
            ++++ RD  IG FPV S   CAK I+KGV R ER+VT+PSWF++ Y+ KV  PE++EW 
Sbjct: 246 VVNQDIRDVQIGAFPVTSVSGCAKGIVKGVCRKERYVTEPSWFKVTYLWKVFCPELIEWG 305

Query: 560 FHVLFVILPNRNKDKSSLQKSISNRNEIKKA 652
             ++F +  +   ++++L K I +   ++ A
Sbjct: 306 CRLMF-LSGHGTPEENALNKKILDIPGVRSA 335


>gb|ACG69524.1| steroleosin SLO1-3 [Brassica napus]
          Length = 341

 Score =  209 bits (533), Expect = 4e-52
 Identities = 97/209 (46%), Positives = 145/209 (69%)
 Frame = +2

Query: 20  VDHMVNNAGIISAFYFEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASV 199
           +DH+VNNAGI     FE   ++T +  VMD NFWGSVY T  ALP+L++S GKI+V++S 
Sbjct: 126 LDHLVNNAGITKISMFENFEEITRTRSVMDTNFWGSVYTTRAALPYLRQSNGKIVVMSSS 185

Query: 200 SGWLYGPNISFYAASKAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKV 379
           + WL  P +SFY ASKAALLNFF+TLR+E G  V +TI +PG+ + E++QGK+ + +G++
Sbjct: 186 AAWLTAPRMSFYNASKAALLNFFETLRIELGGDVHITIVTPGYTQFELAQGKYFSAEGEL 245

Query: 380 EIDKEKRDALIGNFPVRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWF 559
            +D++ RD  +G FPV S   CAK I+ GV R +R+VT+PSWF++ Y+ KV  PE++EW 
Sbjct: 246 VVDQDVRDVQVGAFPVASVSACAKEIVNGVCRKQRYVTEPSWFKVTYLWKVFCPELIEWG 305

Query: 560 FHVLFVILPNRNKDKSSLQKSISNRNEIK 646
             +LF+       ++++L K I +   ++
Sbjct: 306 CRLLFM----TTSEENALNKKILDNTGVR 330