BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00029773
         (1139 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAE16981.1| delta7 sterol C-5 desaturase [Zinnia elegans]         485   e-135
gb|AAD20458.1| sterol-C5(6)-desaturase homolog [Nicotiana tabacum]    483   e-134
gb|ABD96921.1| hypothetical protein [Cleome spinosa]                  482   e-134
ref|XP_002284080.1| PREDICTED: delta(7)-sterol-C5(6)-desaturase ...   479   e-133
ref|XP_002516756.1| sterol desaturase, putative [Ricinus communi...   474   e-131

>dbj|BAE16981.1| delta7 sterol C-5 desaturase [Zinnia elegans]
          Length = 271

 Score =  485 bits (1249), Expect = e-135
 Identities = 215/266 (80%), Positives = 242/266 (90%)
 Frame = -1

Query: 1064 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 885
            L LF+ ET++YNQIVLG ++P  KL+ PLPH+ QTW+RNYI GTL+YLISG LWC YIY+
Sbjct: 5    LSLFVTETTWYNQIVLGTLLP-EKLWTPLPHFLQTWLRNYIAGTLVYLISGFLWCFYIYH 63

Query: 884  LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 705
            LKRNVY+PKDAIP+ KAM LQI VAMKAMPWYCAL  VSEY VENGWTRC+SRISDVGW+
Sbjct: 64   LKRNVYVPKDAIPTRKAMYLQIYVAMKAMPWYCALRAVSEYMVENGWTRCFSRISDVGWA 123

Query: 704  AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 525
            +Y+ Y+ +YL+FVEFGIYWMHRELHDIKPLYK+LHATHHI+NKQNTLSPFAGLAFH +DG
Sbjct: 124  SYISYMTLYLIFVEFGIYWMHRELHDIKPLYKWLHATHHIHNKQNTLSPFAGLAFHAIDG 183

Query: 524  ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 345
            ILQA+PHVIALFL+PTHF THI LLF+EAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR
Sbjct: 184  ILQAIPHVIALFLVPTHFMTHILLLFMEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 243

Query: 344  HNYGHYTVWMDWMFGTLRDPVSDVTK 267
            HNYGHYTVWMDWMFGTLRDP+ +  K
Sbjct: 244  HNYGHYTVWMDWMFGTLRDPLEEEAK 269


>gb|AAD20458.1| sterol-C5(6)-desaturase homolog [Nicotiana tabacum]
          Length = 271

 Score =  483 bits (1244), Expect = e-134
 Identities = 216/266 (81%), Positives = 239/266 (89%)
 Frame = -1

Query: 1064 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 885
            L LF++ETSFYN++VLG  +P S  + PLPHWFQ W+RNYIGG LLY ISG LWC YIY 
Sbjct: 5    LNLFIEETSFYNRVVLGTFLPES-WWGPLPHWFQGWLRNYIGGVLLYFISGFLWCFYIYR 63

Query: 884  LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 705
            LKRNVY+PKDAIPS +AMLLQI VAMKAMP+YCALP++SEY + NGWT+C+SRISDVGW 
Sbjct: 64   LKRNVYIPKDAIPSNRAMLLQIGVAMKAMPFYCALPSLSEYMIVNGWTKCFSRISDVGWL 123

Query: 704  AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 525
            +YL+Y+A+YLV VEFGIYWMHRELHDIK LYKYLHATHHIYNKQNTLSPFAGLAFHPLDG
Sbjct: 124  SYLIYMAVYLVIVEFGIYWMHRELHDIKLLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 524  ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 345
            ILQA+PHV+ALFL+P HFTTHIALLFIEAIWTANIHDC H K WPVMGAGYHTIHHTTYR
Sbjct: 184  ILQAVPHVVALFLLPEHFTTHIALLFIEAIWTANIHDCKHAKVWPVMGAGYHTIHHTTYR 243

Query: 344  HNYGHYTVWMDWMFGTLRDPVSDVTK 267
            HNYGHYT+WMDWMFGTLRDPV D  K
Sbjct: 244  HNYGHYTIWMDWMFGTLRDPVEDEVK 269


>gb|ABD96921.1| hypothetical protein [Cleome spinosa]
          Length = 307

 Score =  482 bits (1240), Expect = e-134
 Identities = 211/273 (77%), Positives = 242/273 (88%)
 Frame = -1

Query: 1094 KNTKMENSTSLKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLIS 915
            K    E S  +  F+DETSFYN+IVLG+++P + L++PLPH+ QTW+RNY+ GTLLYLIS
Sbjct: 25   KTAAAEQSHLMMQFVDETSFYNRIVLGHLLP-ANLWDPLPHFLQTWLRNYLAGTLLYLIS 83

Query: 914  GLLWCLYIYYLKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRC 735
            GL+WC YIYYLK NVY+PKDAIP+ KAMLLQI VAMKAMPWY  LPTVSE+ +E GWTRC
Sbjct: 84   GLIWCFYIYYLKHNVYVPKDAIPTRKAMLLQIYVAMKAMPWYTLLPTVSEHMIERGWTRC 143

Query: 734  YSRISDVGWSAYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPF 555
            +S I D GW+ YLVYLA YLV VEFGIYW+HRELHDIKPLYKYLHATHHIYNKQNTLSPF
Sbjct: 144  FSEIDDYGWTLYLVYLAFYLVIVEFGIYWVHRELHDIKPLYKYLHATHHIYNKQNTLSPF 203

Query: 554  AGLAFHPLDGILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAG 375
            AGLAFHPLDGILQA+PHVIALF++P HFTTH+ALLF+EA+WTANIHDCIHG  WPVMGAG
Sbjct: 204  AGLAFHPLDGILQAVPHVIALFIVPIHFTTHLALLFMEAVWTANIHDCIHGNIWPVMGAG 263

Query: 374  YHTIHHTTYRHNYGHYTVWMDWMFGTLRDPVSD 276
            YHTIHHTTY+HNYGHYT+WMDWMFG+LRDP+ +
Sbjct: 264  YHTIHHTTYKHNYGHYTIWMDWMFGSLRDPIEE 296


>ref|XP_002284080.1| PREDICTED: delta(7)-sterol-C5(6)-desaturase [Vitis vinifera]
            gi|296087259|emb|CBI33633.3| unnamed protein product
            [Vitis vinifera]
          Length = 271

 Score =  479 bits (1233), Expect = e-133
 Identities = 218/260 (83%), Positives = 234/260 (90%)
 Frame = -1

Query: 1064 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 885
            L LF+ ETSFYN+IVLG  +P +KL+E  PH+ QTW+RNYIGGTL+YL+SG LWC YIYY
Sbjct: 5    LHLFVAETSFYNRIVLGTFLP-AKLWETWPHFVQTWLRNYIGGTLIYLVSGFLWCFYIYY 63

Query: 884  LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 705
            LKRNVY+PKDAIP+T+AM LQI VAMKAMPWY ALPT+SEY VENGWTRCYS ISDVGW 
Sbjct: 64   LKRNVYVPKDAIPTTRAMYLQIYVAMKAMPWYTALPTLSEYMVENGWTRCYSSISDVGWP 123

Query: 704  AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 525
            AY V LA+YLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG
Sbjct: 124  AYFVNLAVYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 524  ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 345
            ILQALPHV+ALFL  THF TH+ LLFIEAIWTANIHDCI GK WPVMGA YHTIHHTTYR
Sbjct: 184  ILQALPHVLALFLFRTHFLTHVLLLFIEAIWTANIHDCIDGKIWPVMGAAYHTIHHTTYR 243

Query: 344  HNYGHYTVWMDWMFGTLRDP 285
            HNYGHYTVWMDWMFGTL DP
Sbjct: 244  HNYGHYTVWMDWMFGTLCDP 263


>ref|XP_002516756.1| sterol desaturase, putative [Ricinus communis]
            gi|223544129|gb|EEF45654.1| sterol desaturase, putative
            [Ricinus communis]
          Length = 276

 Score =  474 bits (1221), Expect = e-131
 Identities = 213/263 (80%), Positives = 234/263 (88%)
 Frame = -1

Query: 1064 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 885
            L+ FL+ET+ YN IVLG+++P S  +  LP + QTW+RNYI GTLLY ISG LWC YIYY
Sbjct: 9    LQYFLEETAMYNNIVLGHLLP-SGWWATLPRFLQTWLRNYIAGTLLYFISGFLWCFYIYY 67

Query: 884  LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 705
            LKRNVYLPKDAIP+TKAMLLQI VAMKAMPWYCALP++SEY VENGWT+C+S ISDVG  
Sbjct: 68   LKRNVYLPKDAIPTTKAMLLQIYVAMKAMPWYCALPSLSEYMVENGWTKCFSYISDVGCF 127

Query: 704  AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 525
             Y++YL  YLV VEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG
Sbjct: 128  GYIIYLIAYLVMVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 187

Query: 524  ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 345
            ILQA+PHVIALF++PTHF THI LLF+EAIWTANIHDCIHGK WPVMGAGYHTIHHTTYR
Sbjct: 188  ILQAVPHVIALFIVPTHFRTHIGLLFLEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYR 247

Query: 344  HNYGHYTVWMDWMFGTLRDPVSD 276
            HNYGHYT+WMDWM GTLRDP  D
Sbjct: 248  HNYGHYTIWMDWMLGTLRDPEED 270