BLASTX nr result
ID: Coptis25_contig00028464
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00028464
(977 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI25465.3| unnamed protein product [Vitis vinifera] 120 6e-25
ref|XP_002272219.1| PREDICTED: gibberellin 2-beta-dioxygenase 1-... 120 6e-25
ref|XP_003616656.1| Gibberellin 2-beta-dioxygenase [Medicago tru... 109 9e-22
ref|XP_002311230.1| 2-oxoglutarate-dependent dioxygenase [Populu... 108 3e-21
ref|XP_002528301.1| conserved hypothetical protein [Ricinus comm... 107 6e-21
>emb|CBI25465.3| unnamed protein product [Vitis vinifera]
Length = 380
Score = 120 bits (300), Expect = 6e-25
Identities = 58/102 (56%), Positives = 76/102 (74%)
Frame = -2
Query: 919 KFTSELEKVGTDVVEALSSAMGFSKRLKEKDIQFSSLMWVSEGVVGNKQMISGRFYPYII 740
+FT +EK+G DV+++L+ A+GF + + LMW+SE V GNK +SGR YPYII
Sbjct: 165 EFTRAMEKLGLDVLDSLARAVGFENPFRSGSNRVCPLMWISEDVPGNKPDLSGRVYPYII 224
Query: 739 ALQYQITCRKYSVLTDSGWSSVSPQVDSVLVTVGDIAQVQPN 614
LQYQIT +K+S+L DSGW +VSP+VDSV+VTVGDIAQV N
Sbjct: 225 GLQYQITSQKHSLLVDSGWITVSPRVDSVMVTVGDIAQVWSN 266
>ref|XP_002272219.1| PREDICTED: gibberellin 2-beta-dioxygenase 1-like [Vitis vinifera]
Length = 382
Score = 120 bits (300), Expect = 6e-25
Identities = 58/102 (56%), Positives = 76/102 (74%)
Frame = -2
Query: 919 KFTSELEKVGTDVVEALSSAMGFSKRLKEKDIQFSSLMWVSEGVVGNKQMISGRFYPYII 740
+FT +EK+G DV+++L+ A+GF + + LMW+SE V GNK +SGR YPYII
Sbjct: 165 EFTRAMEKLGLDVLDSLARAVGFENPFRSGSNRVCPLMWISEDVPGNKPDLSGRVYPYII 224
Query: 739 ALQYQITCRKYSVLTDSGWSSVSPQVDSVLVTVGDIAQVQPN 614
LQYQIT +K+S+L DSGW +VSP+VDSV+VTVGDIAQV N
Sbjct: 225 GLQYQITSQKHSLLVDSGWITVSPRVDSVMVTVGDIAQVWSN 266
Score = 82.8 bits (203), Expect = 1e-13
Identities = 46/121 (38%), Positives = 66/121 (54%), Gaps = 15/121 (12%)
Frame = -3
Query: 462 KVWSNGKLKKVRGRPVATSGND---NSRFITMSXXXXXXXXXXXTIS-PFLPVI------ 313
+VWSNGKLKKVRGR V G++ +SR ++M+ P LP +
Sbjct: 262 QVWSNGKLKKVRGRAVVNRGSEAGKSSRSVSMALLLTLPLDTPNVSPLPLLPHLPPADHE 321
Query: 312 -----SSHANDSANDEGQENDKVDKVTQEKMIFDTFSFEDYAWRVYHERFLFKDPLDRYL 148
+ ++ + G ++ ++ E+ +F +FSFEDYAWR+YHER L KDPLDRY
Sbjct: 322 TQPHKAEEQTEAGSTSGTSKEEEEEEDDERPLFRSFSFEDYAWRIYHERLLLKDPLDRYR 381
Query: 147 I 145
I
Sbjct: 382 I 382
>ref|XP_003616656.1| Gibberellin 2-beta-dioxygenase [Medicago truncatula]
gi|355517991|gb|AES99614.1| Gibberellin
2-beta-dioxygenase [Medicago truncatula]
Length = 379
Score = 109 bits (273), Expect = 9e-22
Identities = 53/103 (51%), Positives = 73/103 (70%), Gaps = 1/103 (0%)
Frame = -2
Query: 919 KFTSELEKVGTDVVEALSSAMGFSKRLKEKDIQFSSLMWVSEGVVGNKQMISGRFYPYII 740
+F LEK+G ++++ L + +G + + +FSS+MWVSE + GNK G FYP+I+
Sbjct: 175 EFACALEKLGLNIIDVLMNGLGVENPVGDDSNRFSSIMWVSECLPGNKPGSMGGFYPFIV 234
Query: 739 ALQYQITCRKYSVLTDS-GWSSVSPQVDSVLVTVGDIAQVQPN 614
LQYQI C+KYS+L+DS GW SV P VDS+LVTVGD+AQV N
Sbjct: 235 GLQYQIRCQKYSLLSDSGGWVSVLPHVDSILVTVGDVAQVWSN 277
Score = 92.4 bits (228), Expect = 1e-16
Identities = 52/108 (48%), Positives = 68/108 (62%), Gaps = 2/108 (1%)
Frame = -3
Query: 462 KVWSNGKLKKVRGRPV-ATSGNDN-SRFITMSXXXXXXXXXXXTISPFLPVISSHANDSA 289
+VWSNGKLKKVRGRP+ A G++N S ITMS ++P LP+ + + +
Sbjct: 273 QVWSNGKLKKVRGRPIMAALGDENDSSCITMSLLITLPLESN--VAPLLPIGNKNKVEDD 330
Query: 288 NDEGQENDKVDKVTQEKMIFDTFSFEDYAWRVYHERFLFKDPLDRYLI 145
D +E + + Q K +F++ FEDYAWRVYHER LFKDPLDRY I
Sbjct: 331 IDNDEEENNIGGEGQ-KRVFNSIDFEDYAWRVYHERLLFKDPLDRYRI 377
>ref|XP_002311230.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
gi|222851050|gb|EEE88597.1| 2-oxoglutarate-dependent
dioxygenase [Populus trichocarpa]
Length = 357
Score = 108 bits (269), Expect = 3e-21
Identities = 56/104 (53%), Positives = 73/104 (70%), Gaps = 2/104 (1%)
Frame = -2
Query: 919 KFTSELEKVGTDVVEALSSAMGFSKRLKEKDIQFSSLMWVSEGVVGN--KQMISGRFYPY 746
+ T LEK+G +V++ LS+ GF LKE + SL+ + G+ GN K +SG YPY
Sbjct: 151 ELTRALEKLGLEVIQMLSNGAGFENPLKEHPTRNYSLLCLHGGLDGNDDKPGLSGGSYPY 210
Query: 745 IIALQYQITCRKYSVLTDSGWSSVSPQVDSVLVTVGDIAQVQPN 614
I+ LQYQI C+KYS+LTDSGW +V PQVDS++VTVGDIAQV N
Sbjct: 211 IVGLQYQIRCQKYSLLTDSGWVTVLPQVDSIMVTVGDIAQVWSN 254
Score = 92.4 bits (228), Expect = 1e-16
Identities = 52/110 (47%), Positives = 67/110 (60%), Gaps = 4/110 (3%)
Frame = -3
Query: 462 KVWSNGKLKKVRGRPVATSGN-DNSRFITMSXXXXXXXXXXXTISPFLPVISS---HAND 295
+VWSNGKLKKVRGRP A G+ +NSR I+MS +SP LP + + +AN+
Sbjct: 250 QVWSNGKLKKVRGRPKACLGDCENSRCISMSLLVTLPSEST--VSPLLPKVITDGINANE 307
Query: 294 SANDEGQENDKVDKVTQEKMIFDTFSFEDYAWRVYHERFLFKDPLDRYLI 145
E +E D + V + F +F F+DYAWRVYH LFKDPLD+Y I
Sbjct: 308 DEIREDEEQDNIHSVCKTGRRFGSFPFDDYAWRVYHGPLLFKDPLDKYRI 357
>ref|XP_002528301.1| conserved hypothetical protein [Ricinus communis]
gi|223532256|gb|EEF34059.1| conserved hypothetical
protein [Ricinus communis]
Length = 435
Score = 107 bits (266), Expect = 6e-21
Identities = 57/103 (55%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Frame = -2
Query: 919 KFTSELEKVGTDVVEALSSAMGFSKRLKEKDIQFSSLMWVSEGVVGNK-QMISGRFYPYI 743
+ T LEK+G + +E LS A+GF K + SS+M V EG G+K ISG FYPY+
Sbjct: 223 ELTRGLEKLGLEAMEMLSKAVGFENPFKAYPTRLSSMMSVHEGSYGDKPDHISGGFYPYV 282
Query: 742 IALQYQITCRKYSVLTDSGWSSVSPQVDSVLVTVGDIAQVQPN 614
+ LQY+I RKY +L DSGW +V PQVDSVLVTVGDIAQV N
Sbjct: 283 VGLQYEIRSRKYWLLADSGWVAVVPQVDSVLVTVGDIAQVWSN 325
Score = 100 bits (248), Expect = 7e-19
Identities = 55/115 (47%), Positives = 75/115 (65%), Gaps = 9/115 (7%)
Frame = -3
Query: 462 KVWSNGKLKKVRGRPVATSGND-NSRFITMSXXXXXXXXXXXTISPFLPVISSH--ANDS 292
+VWSNGKLK+VRGRP+ G+ +S ITM+ ISP LP + +H A+D
Sbjct: 321 QVWSNGKLKRVRGRPLPCMGDGKDSSCITMTLLLTLPTETT--ISPLLPELDTHNSADDE 378
Query: 291 ANDEGQENDKVDK------VTQEKMIFDTFSFEDYAWRVYHERFLFKDPLDRYLI 145
+E +E ++ D+ V +EK +F++FSFEDYAWRVYHE F+FKDPLD+Y I
Sbjct: 379 VREEEEEEEEEDEKDGKNVVKKEKRMFNSFSFEDYAWRVYHEPFIFKDPLDKYRI 433