BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00027422
         (1813 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   580   e-163
ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|2...   578   e-162
ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago...   570   e-160
ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   561   e-157
gb|AFK34722.1| unknown [Lotus japonicus]                              551   e-154

>ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial [Vitis
            vinifera] gi|297741744|emb|CBI32876.3| unnamed protein
            product [Vitis vinifera]
          Length = 424

 Score =  580 bits (1494), Expect = e-163
 Identities = 293/428 (68%), Positives = 342/428 (79%)
 Frame = +2

Query: 290  MLRHTLRRTLKSCTSSVWIQSRKSTIPSQSLLLETIPKENVDCXXXXXXXXXXXXXRDLA 469
            MLRH +R    S     WI S+  T  S+  +   + +E+VDC             R+LA
Sbjct: 1    MLRHAIRGLRNSRR---WISSKNFT--SRVGVERAVARESVDCVVIGAGVVGIAVARELA 55

Query: 470  LKGRDVLVIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCLRGRKLLYQYCANHGVP 649
            LKGR+V+VIE A TFG+GTSSRNSEVIHAGIYYP NSLKAIFC RGR+LLY+YC+ H VP
Sbjct: 56   LKGREVVVIEFASTFGTGTSSRNSEVIHAGIYYPRNSLKAIFCARGRELLYKYCSEHEVP 115

Query: 650  HKQIGKLIVATGSSEIQKLIVLLSQGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYS 829
            HKQIGKLIVAT SSE+ KL  L+ +G ENGV+GLRM+EGFEAM MEPEL+C++ALLSP S
Sbjct: 116  HKQIGKLIVATRSSEVPKLNDLMIRGNENGVDGLRMLEGFEAMEMEPELQCVRALLSPAS 175

Query: 830  GIVDTHALMLSLVGEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQP 1009
            GIVDTH+LMLSLVGEAENH  TF YNT V+GGHLEGD I +HIS+SK++E      P  P
Sbjct: 176  GIVDTHSLMLSLVGEAENHRTTFCYNTAVIGGHLEGDHICLHISESKDLENWDRRFPLHP 235

Query: 1010 ELILVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYAQGCYFTLSNNKTPPFEHLIYPI 1189
            E+IL+  LVVNSAGLS+P+LAK F GL+S VIP S+YA+GCYF LS  KTPPF+HLIYPI
Sbjct: 236  EVILMAKLVVNSAGLSAPALAKRFDGLNSTVIPASHYARGCYFVLSKMKTPPFKHLIYPI 295

Query: 1190 PEDGGLGVHVTLDLDGHVKFGPDVEWIGELDSISSFLNKFDYSVSSDRVQKFYPEIRRYY 1369
            PEDGGLGVHVTLDLDG +KFGPDVEWI   D ISSFLNKFDYSV + RV++FYPEIR+YY
Sbjct: 296  PEDGGLGVHVTLDLDGQIKFGPDVEWINGADDISSFLNKFDYSVCAKRVERFYPEIRKYY 355

Query: 1370 PNLKEGSLEPGYAGIRPKLSGPGQPPVDFIIQGEDINGVRGLINLFGIESPGLTSSLAIA 1549
            PNLK+ SLEPGYAGIRPKLSG  Q  VDF+IQGEDI+GV GL+NLFGIESPGLTSS+AIA
Sbjct: 356  PNLKDDSLEPGYAGIRPKLSGAQQSAVDFVIQGEDIHGVPGLVNLFGIESPGLTSSMAIA 415

Query: 1550 EHIGLKFI 1573
            EHI  +++
Sbjct: 416  EHIVARYL 423


>ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|222854820|gb|EEE92367.1|
            predicted protein [Populus trichocarpa]
          Length = 430

 Score =  578 bits (1489), Expect = e-162
 Identities = 282/426 (66%), Positives = 349/426 (81%), Gaps = 3/426 (0%)
 Frame = +2

Query: 305  LRRTLKSCTSSVWIQSRKSTIPSQSLLL--ETIPKENVDCXXXXXXXXXXXXXRDLALKG 478
            L++TL+S   +  I S+   I + S  +  E +P E +DC             R+LA KG
Sbjct: 5    LKQTLQSLKRTATI-SKAQAIKNLSTQIKKEDVPTEKLDCVVIGAGVVGIAIARELASKG 63

Query: 479  RDVLVIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCLRGRKLLYQYCANHGVPHKQ 658
            R+VLV++SA TFG+GTSSRNSEVIHAGIYYPP+SLKA+FC+RGR+LLY+YC  HGVP KQ
Sbjct: 64   REVLVLDSASTFGTGTSSRNSEVIHAGIYYPPDSLKALFCVRGRELLYRYCYEHGVPCKQ 123

Query: 659  IGKLIVATGSSEIQKLIVLLSQGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGIV 838
            IGKLIVATG  EI KL  L+ +G +NGV+ L+M+EGFEAM+MEPEL+C KALLSP SGIV
Sbjct: 124  IGKLIVATGPLEISKLNELMDRGTQNGVDNLKMLEGFEAMKMEPELQCKKALLSPVSGIV 183

Query: 839  DTHALMLSLVGEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQPELI 1018
            D+H+LMLSLVGEAEN+G TFSYN+TV+ GHLEG+ + ++I +SK++E   G  P  PEL+
Sbjct: 184  DSHSLMLSLVGEAENNGTTFSYNSTVISGHLEGNCLHLYIVESKDLENWNGDYPLHPELV 243

Query: 1019 LVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYAQGCYFTLSNNKTPPFEHLIYPIPED 1198
            LVP LVVNSAGLSS +LAK F GLD+ +IPP ++A+GCYFTLS+ K PPFEHLIYPIPED
Sbjct: 244  LVPKLVVNSAGLSSLALAKRFHGLDNGIIPPGFFARGCYFTLSSTKVPPFEHLIYPIPED 303

Query: 1199 GGLGVHVTLDLDGHVKFGPDVEWIGELDSISSFLNKFDYSVSSDRVQKFYPEIRRYYPNL 1378
            GGLGVHVTLDLDGH+KFGPDVEWI  +D +SSFLNK+DYSVS+ R ++FYPEIR+YYPNL
Sbjct: 304  GGLGVHVTLDLDGHLKFGPDVEWIDGIDDVSSFLNKYDYSVSASRAERFYPEIRKYYPNL 363

Query: 1379 KEGSLEPGYAGIRPKLSGPGQPPVDFII-QGEDINGVRGLINLFGIESPGLTSSLAIAEH 1555
            K+GSL+PGY+GIRPK+SGP Q P+DF+I QGEDI+GV GL+NLFGIESPGLT+S+AIAEH
Sbjct: 364  KDGSLQPGYSGIRPKISGPRQSPIDFVIQQGEDIHGVPGLVNLFGIESPGLTASMAIAEH 423

Query: 1556 IGLKFI 1573
            I  +F+
Sbjct: 424  IASRFL 429


>ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago truncatula]
            gi|355493920|gb|AES75123.1| L-2-hydroxyglutarate
            dehydrogenase [Medicago truncatula]
          Length = 483

 Score =  570 bits (1469), Expect = e-160
 Identities = 279/446 (62%), Positives = 349/446 (78%), Gaps = 25/446 (5%)
 Frame = +2

Query: 311  RTLKSCTSSVWIQSRKSTIPSQSLLLETIPKENVDCXXXXXXXXXXXXXRDLALKGRDVL 490
            R ++S T +    +    +  ++    ++P+E VDC             R LALKGR+V+
Sbjct: 38   RNIRSTTQNDTNTTTSYPVSRETTTSYSVPRERVDCVVIGAGVVGIAVARALALKGREVI 97

Query: 491  VIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCLRGRKLLYQYCANHGVPHKQIGKL 670
            VIESAP+FG+GTSSRNSEV+HAGIYYP +SLKAIFC++GR++LY+YCA H +PH+Q GKL
Sbjct: 98   VIESAPSFGTGTSSRNSEVVHAGIYYPHHSLKAIFCVKGREMLYEYCAKHDIPHEQTGKL 157

Query: 671  IVATGSSEIQKLIVLLSQGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHA 850
            IVAT SSEI KL V+L+ G +NGV+GL+MM+G +AM+MEPEL+C+KA+LSP SGIVD+H+
Sbjct: 158  IVATRSSEIPKLSVILNHGIQNGVDGLKMMDGVDAMKMEPELQCVKAILSPLSGIVDSHS 217

Query: 851  LMLSLV-------------------------GEAENHGATFSYNTTVVGGHLEGDSIQIH 955
            LMLSLV                         GEAENHGATF+YN+TV+GGH+EG+ I +H
Sbjct: 218  LMLSLVVCISSFYYHVVKLDLVLNINPMLVQGEAENHGATFTYNSTVIGGHMEGNEICLH 277

Query: 956  ISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYAQGCY 1135
            +S++K++++  G    QPEL+L+P LVVNSAGLS+ +LAK F GL+++VIPP+YYA+GCY
Sbjct: 278  VSETKSLKEWNGKSSLQPELVLIPKLVVNSAGLSALALAKRFTGLENKVIPPAYYARGCY 337

Query: 1136 FTLSNNKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELDSISSFLNKFDY 1315
            FTLSN K  PF HLIYPIPEDGGLGVHVTLDL+G VKFGPDVEWI  +D ISSF NKFDY
Sbjct: 338  FTLSNTKASPFRHLIYPIPEDGGLGVHVTLDLNGQVKFGPDVEWIDGVDDISSFQNKFDY 397

Query: 1316 SVSSDRVQKFYPEIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFIIQGEDINGVRGL 1495
            SV ++R +KFYPEIR+YYPNLK+GSLEPGY+GIRPKLSGP Q PVDF+IQGEDI+GV GL
Sbjct: 398  SVQANRAEKFYPEIRKYYPNLKDGSLEPGYSGIRPKLSGPCQSPVDFVIQGEDIHGVPGL 457

Query: 1496 INLFGIESPGLTSSLAIAEHIGLKFI 1573
            INLFGIESPGLTSSLAIA+ I  KF+
Sbjct: 458  INLFGIESPGLTSSLAIADFISTKFL 483


>ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial-like
            [Glycine max]
          Length = 410

 Score =  561 bits (1446), Expect = e-157
 Identities = 267/395 (67%), Positives = 329/395 (83%)
 Frame = +2

Query: 389  ETIPKENVDCXXXXXXXXXXXXXRDLALKGRDVLVIESAPTFGSGTSSRNSEVIHAGIYY 568
            +++PKE V+C             R LALKGR+VLV+ESAPTFG+GTSSRNSEVIHAGIYY
Sbjct: 16   DSVPKERVECVVIGAGVVGIAVARALALKGREVLVVESAPTFGTGTSSRNSEVIHAGIYY 75

Query: 569  PPNSLKAIFCLRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKLIVLLSQGRENGVEG 748
            P NS KAIFC+RGR++LY+YC+ H +PHKQIGKLIVAT SSEI KL  +L+ G +NGV+G
Sbjct: 76   PLNSFKAIFCVRGREMLYEYCSKHDIPHKQIGKLIVATRSSEIPKLNDILNCGIQNGVDG 135

Query: 749  LRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLVGEAENHGATFSYNTTVVGGH 928
            L++++G EAM+MEPEL+C+KA+LSP +GIVD+H+LMLSLVGEAEN G TF+YN+TV+GGH
Sbjct: 136  LKIVDGVEAMKMEPELQCVKAILSPLTGIVDSHSLMLSLVGEAENQGTTFTYNSTVIGGH 195

Query: 929  LEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRVIP 1108
            LEG  I +H++++  + +  G    QPEL+L+P LVVNS GLSSP LAK F G  S V+P
Sbjct: 196  LEGSEICLHVTETDRLTEWKGTSILQPELLLIPKLVVNSTGLSSPVLAKRFNGPKSGVVP 255

Query: 1109 PSYYAQGCYFTLSNNKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELDSI 1288
            P+YYA+GCYFTLSN K  PF  LIYPIPEDGG+GVHVT+DL+G VKFGP+VEWI  +D I
Sbjct: 256  PAYYARGCYFTLSNTKNSPFRRLIYPIPEDGGIGVHVTIDLNGQVKFGPNVEWIDSVDDI 315

Query: 1289 SSFLNKFDYSVSSDRVQKFYPEIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFIIQG 1468
            SSF NK+DYSV+++R ++FYPEIR+YYPNLK+GSLEPGY+GIRPKLSGP QPP DF+IQG
Sbjct: 316  SSFQNKYDYSVNANRAERFYPEIRKYYPNLKDGSLEPGYSGIRPKLSGPLQPPSDFVIQG 375

Query: 1469 EDINGVRGLINLFGIESPGLTSSLAIAEHIGLKFI 1573
            EDI+GV GLINLFGIESPGLTSS+AIAE I  +F+
Sbjct: 376  EDIHGVPGLINLFGIESPGLTSSMAIAEFISTRFL 410


>gb|AFK34722.1| unknown [Lotus japonicus]
          Length = 418

 Score =  551 bits (1421), Expect = e-154
 Identities = 265/398 (66%), Positives = 325/398 (81%)
 Frame = +2

Query: 383  LLETIPKENVDCXXXXXXXXXXXXXRDLALKGRDVLVIESAPTFGSGTSSRNSEVIHAGI 562
            +++++P+E VDC             R LALKGR+VLVIES  TFG+GTSSRNSEVIHAGI
Sbjct: 21   IMDSVPREKVDCVVIGAGVVGIAVARALALKGREVLVIESGSTFGTGTSSRNSEVIHAGI 80

Query: 563  YYPPNSLKAIFCLRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKLIVLLSQGRENGV 742
            YYP +SLKAIFC+RGR++LY+YC+ H +PHKQ+GKLIVAT SSEI KL  +L+ G +NGV
Sbjct: 81   YYPRDSLKAIFCVRGREMLYEYCSMHDIPHKQVGKLIVATRSSEIPKLNDILNHGIQNGV 140

Query: 743  EGLRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLVGEAENHGATFSYNTTVVG 922
            + L M+ G +AM+MEPEL+C+KA+LSP SGIVD+H+LMLSLVGEAENH  TF+YN+TV+G
Sbjct: 141  DSLEMINGAKAMKMEPELQCVKAILSPLSGIVDSHSLMLSLVGEAENHRTTFTYNSTVIG 200

Query: 923  GHLEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRV 1102
            GHLEG  I +HI ++K +++  G     PELILVP L+VNS GLS+P+LAK F  L S V
Sbjct: 201  GHLEGKEICLHILETKCLKEWDGTSILHPELILVPKLLVNSTGLSAPALAKRFTSLQSEV 260

Query: 1103 IPPSYYAQGCYFTLSNNKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELD 1282
            IPPSYYA+GCYFTLS+ K  PF HLIYPIPEDGGLGVHVTLDL+G +KFGPDVEWI  +D
Sbjct: 261  IPPSYYARGCYFTLSDTKASPFRHLIYPIPEDGGLGVHVTLDLNGQIKFGPDVEWIDGVD 320

Query: 1283 SISSFLNKFDYSVSSDRVQKFYPEIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFII 1462
             ISSF NKFDYSV ++R ++FYP IR YYPNLK+ +LEPGYAGIRPKLS PGQ PVDF+I
Sbjct: 321  DISSFQNKFDYSVHANRAEQFYPAIRSYYPNLKDRALEPGYAGIRPKLSRPGQSPVDFVI 380

Query: 1463 QGEDINGVRGLINLFGIESPGLTSSLAIAEHIGLKFIG 1576
            QGEDI+G+ GL+NLFGIESPGLT+SLAIA+ +  + +G
Sbjct: 381  QGEDIHGIPGLVNLFGIESPGLTASLAIADFVSTRLLG 418