BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00027281
         (1181 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABD28730.1| Ribonuclease H [Medicago truncatula]                    83   2e-31
ref|XP_003621690.1| Cytochrome c biogenesis protein ccsA [Medica...    80   3e-29
ref|XP_003605842.1| Glycerol-3-phosphate dehydrogenase [Medicago...    74   5e-19
emb|CCA66140.1| hypothetical protein [Beta vulgaris subsp. vulga...    62   2e-16
ref|XP_003605652.1| GDSL esterase/lipase [Medicago truncatula] g...    89   2e-15

>gb|ABD28730.1| Ribonuclease H [Medicago truncatula]
          Length = 409

 Score = 82.8 bits (203), Expect(2) = 2e-31
 Identities = 45/153 (29%), Positives = 78/153 (50%), Gaps = 1/153 (0%)
 Frame = -2

Query: 1150 DYVVWCPDMKGNFSTRSAYEEIRTKKQVISWSKCILNNYIHLRPAAIAWKILQKSVATDE 971
            D ++W   + G+ S + A+  +      + W+K + N Y     A I W+ L   + TD+
Sbjct: 41   DKLIWTDSVDGDLSNKLAFSFLPGHGPTVHWAKMLWNAYTPPTGAFITWRFLHNKLPTDD 100

Query: 970  RVQKLGVHMAS-CCRLCRQCPETHSHLFWLCCFSQKLWQWLAKAFNTSHIVCLDDALKVN 794
             ++K G ++ S CC  CR+  ET SH+F  C  + +LW WL KA  T   +     L + 
Sbjct: 101  NLRKRGCYIVSICCCFCRKQAETSSHIFLQCPVTLQLWDWLLKA--TDQHLDFSSILNI- 157

Query: 793  SQWSPRLKQLWHTCIVAMLTSIWKQRNLIHFEG 695
               S  ++ + ++ IV ++ SIW + N  +F+G
Sbjct: 158  ---SRMVQHVMNSAIVHIMWSIWLECNNKYFDG 187



 Score = 80.9 bits (198), Expect(2) = 2e-31
 Identities = 50/155 (32%), Positives = 75/155 (48%), Gaps = 3/155 (1%)
 Frame = -3

Query: 585 KFWDVASKIRNAPTVKQCIWIPPAPNILKLNTDGASLGNPRPAGIGISYRDNMGGFKLTV 406
           + + +  K       ++ IW+PP    +K+N DG+ +G+P    IG+ +R +   F    
Sbjct: 226 RLFSIPFKTNRVNPCREIIWVPPHGGCMKINCDGSVVGSPSCGSIGVIFRASQTMFCGAF 285

Query: 405 WKSIGVGTNFWAECMAILEGVEIAMSKGWYKL*IETDSEAAAQVFR-SGNVPWTLQTRWN 229
            ++IG  T   AE  A +  +E A       + IETDS    + F  +  VPW +  RW+
Sbjct: 286 AQNIGYATALEAEYSACMFAIEKAKELHLTNIWIETDSVNVIRAFHFNTGVPWKMHIRWH 345

Query: 228 NCTLAVPDIK--ITSIFREANYTADRLAKIGALLA 130
           NC L    I+   T + RE N  AD LAK G  LA
Sbjct: 346 NCLLFCRSIRSLCTHVNREGNLVADALAKNGQGLA 380


>ref|XP_003621690.1| Cytochrome c biogenesis protein ccsA [Medicago truncatula]
           gi|355496705|gb|AES77908.1| Cytochrome c biogenesis
           protein ccsA [Medicago truncatula]
          Length = 666

 Score = 79.7 bits (195), Expect(2) = 3e-29
 Identities = 52/153 (33%), Positives = 72/153 (47%), Gaps = 3/153 (1%)
 Frame = -3

Query: 591 VIKFWDVASKIRNAPTVKQCIWIPPAPNILKLNTDGASLGNPRPAGIGISYRDNMGGFKL 412
           V K +++  K++        IW PP  +I+K+N DG+S+G      IGI  RD+   F  
Sbjct: 195 VAKLFNIPFKVKRVTPHLDIIWKPPIGDIVKINCDGSSVGRHPCGSIGIVIRDSNHHFLG 254

Query: 411 TVWKSIGVGTNFWAECMAILEGVEIAMSKGWYKL*IETDSEAAAQVFRSG-NVPWTLQTR 235
            +  +IG  T   AE  A +  +E A       + +ETDS      F  G  VPW ++ R
Sbjct: 255 AISSNIGNATPLEAEFCAGMMAMEKAQEMQLMHVCLETDSLKVVNAFNKGLGVPWQMRAR 314

Query: 234 WNNCTLAVPDIKIT--SIFREANYTADRLAKIG 142
           W NC      I  +   I RE N  AD LAK G
Sbjct: 315 WQNCWDFCDSISCSCVHILREGNMVADALAKHG 347



 Score = 76.3 bits (186), Expect(2) = 3e-29
 Identities = 44/165 (26%), Positives = 79/165 (47%), Gaps = 4/165 (2%)
 Frame = -2

Query: 1120 GNFSTRSAYEEIRTKKQVISWSKCILNNYIHLRPAAIAWKILQKSVATDERVQKLGVHMA 941
            G+ + + AY+ I      + W K + N+YI    + I W++L   + TDE ++K G  + 
Sbjct: 16   GDLTNQLAYKFINETGNHVLWDKFLWNSYIPPSRSFITWRLLHNKLPTDENLRKRGCLIV 75

Query: 940  SCCRLCRQCPETHSHLFWLCCFSQKLWQWLAKAFNTSHIV----CLDDALKVNSQWSPRL 773
            S C  C +  E+  H+F+ C  + +LW WL K   T  ++    CL   ++     S  +
Sbjct: 76   SICCFCMKSAESSQHIFFECHVTSRLWDWLGK--GTDKLLDCSSCLQLLIRNWGSGSKLV 133

Query: 772  KQLWHTCIVAMLTSIWKQRNLIHFEGRTGNIRDCKYYIRTQVKLS 638
              + ++ I+  + SIW +RN   F  +   +      I  +VK+S
Sbjct: 134  NNILNSAIIHTIWSIWIERNQRCFHNKHQAMTTLFNIILAEVKMS 178


>ref|XP_003605842.1| Glycerol-3-phosphate dehydrogenase [Medicago truncatula]
            gi|355506897|gb|AES88039.1| Glycerol-3-phosphate
            dehydrogenase [Medicago truncatula]
          Length = 866

 Score = 73.9 bits (180), Expect(2) = 5e-19
 Identities = 43/170 (25%), Positives = 85/170 (50%), Gaps = 2/170 (1%)
 Frame = -2

Query: 1126 MKGNFSTRSAYEEIRTKKQVISWSKCILNNYIHLRPAAIAWKILQKSVATDERVQKLGVH 947
            ++G  +  S  ++ + K+  ++  K I +  I    + +AW+++   +  DE++++ G  
Sbjct: 23   VRGEKAVESFVDQSKQKEDELA--KSIWSPNIPPSKSLLAWRLMNDKIPVDEKLKERGHV 80

Query: 946  MASCCRLCRQCPETHSHLFWLCCFSQKLWQWLAKAFNTS-HIVCLDDALKVNSQ-WSPRL 773
            +   C LC    E+  HLF+ C ++  LW WLA   N S H + L++   ++++ WSP+ 
Sbjct: 81   IPFICSLCMTAEESSFHLFFECSYAVHLWCWLASILNQSLHFLALEEIWSLSARGWSPQF 140

Query: 772  KQLWHTCIVAMLTSIWKQRNLIHFEGRTGNIRDCKYYIRTQVKLSGHLAK 623
                 + ++ +L++IW  RN   F+ R  +       I   V LS   +K
Sbjct: 141  TLAITSTVINILSAIWNARNTYRFKNRKIHWESVISQIIVNVSLSASNSK 190



 Score = 47.8 bits (112), Expect(2) = 5e-19
 Identities = 45/155 (29%), Positives = 63/155 (40%), Gaps = 3/155 (1%)
 Frame = -3

Query: 600 ETGVIKFWDVASKIRNAPTVKQCIWIPPAPNILKLNTDGASLGNPRPAGIGISYRDNMGG 421
           E  ++K ++V+        VK+ I  PP    +K N DGA       A  G  +R++ G 
Sbjct: 199 EFSILKAFNVSIHPLKLLIVKEVISSPPISGWIKGNCDGAFAAGK--AACGAIFRNSKGQ 256

Query: 420 FKLTVWKSIGVGTNFWAECMAILEGVEIAMSKGWYKL*IETDSEAAAQVFRSGNV-PWTL 244
           F                 C A  EG+             ETD     Q F+S ++ PW L
Sbjct: 257 FM---------------GCFA--EGLNN-----------ETDFMLVVQAFKSQDIFPWQL 288

Query: 243 QTRWNNCTLAVPDIK--ITSIFREANYTADRLAKI 145
             RW+NC      +   ++ IFRE N  AD LA I
Sbjct: 289 TNRWSNCLNITSSMNFIVSHIFREGNACADALANI 323


>emb|CCA66140.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
          Length = 1381

 Score = 61.6 bits (148), Expect(2) = 2e-16
 Identities = 47/171 (27%), Positives = 71/171 (41%), Gaps = 12/171 (7%)
 Frame = -2

Query: 1150 DYVVWCPDMKGNFSTRSAYEEIRTKKQVISWSKCILNNYIHLRPAAI---AWKILQKSVA 980
            D ++W     G FST+S   E+  K         I   +  L P  I    W  L   ++
Sbjct: 1014 DQLIWAYSSSGKFSTKSFSLEV-DKLSPPPHHDAINGVWRGLVPHRIEIFVWMALLGKIS 1072

Query: 979  TDERVQKLGVHMAS--CCRLCRQCPETHSHLFWLCCFSQKLWQWLAKAFNTSHIV--CLD 812
            T  ++ K+G+       C LC    ET  HL   C F++ LW W    +N   +    L 
Sbjct: 1073 TKHKLAKIGIIPKDDDICILCSNSSETSDHLLLHCNFARSLWHWWFSLWNIQWVFPHTLR 1132

Query: 811  DALKVNSQWSPR-----LKQLWHTCIVAMLTSIWKQRNLIHFEGRTGNIRD 674
            +A     QW  R      K+ W T    ++ S+WK+RN   FE    +++D
Sbjct: 1133 EAF---DQWQTRSRCVFFKKAWLTIFFIIVWSVWKERNSRIFEKSESSVKD 1180



 Score = 51.2 bits (121), Expect(2) = 2e-16
 Identities = 44/155 (28%), Positives = 68/155 (43%), Gaps = 9/155 (5%)
 Frame = -3

Query: 579  WDVASKIRNAPTVKQC--IWIPPAPNILKLNTDGASLGNPRPAGIGISYRDNMGGFKLTV 406
            W+ A+ +   P ++ C  +W PP  N LK N D ++      + +G   R++ G F    
Sbjct: 1216 WNGANSLMQYPKLQPCPIVWTPPIENFLKWNVDASANPLLSTSAMGGVLRNSQGNFMCLF 1275

Query: 405  WKSIGVGTNFWAECMAILEGVEIAMSKGWYK---L*IETDS-EAAAQVFRSGNVPWTLQT 238
               I       AE + I   V+I++S    K   L IE+DS  A +   +    PW +  
Sbjct: 1276 SSPIPFMEINCAEILGIYRAVKISISSDCIKEKNLIIESDSANAVSWCNQDEGGPWNMNF 1335

Query: 237  RWN---NCTLAVPDIKITSIFREANYTADRLAKIG 142
            + N   N       + IT   R AN+ AD +AK G
Sbjct: 1336 QLNFIRNARKKNLRLTITHERRSANFVADSMAKQG 1370


>ref|XP_003605652.1| GDSL esterase/lipase [Medicago truncatula]
            gi|355506707|gb|AES87849.1| GDSL esterase/lipase
            [Medicago truncatula]
          Length = 1004

 Score = 89.0 bits (219), Expect = 2e-15
 Identities = 45/148 (30%), Positives = 76/148 (51%), Gaps = 2/148 (1%)
 Frame = -2

Query: 1150 DYVVWCPDMKGNFSTRSAYEEIRTKKQVISWSKCILNNYIHLRPAAIAWKILQKSVATDE 971
            D ++W  +  G+   R +Y          +W+K I +  I    + + W+++   V TDE
Sbjct: 82   DKLIWNHNSNGDLMMRDSYAFKNQPVNTHNWAKLIWSKDIPPSKSILTWRLIHDKVPTDE 141

Query: 970  RVQKLGVHMASCCRLCRQCPETHSHLFWLCCFSQKLWQWLAKAFNTS-HIVCLDDALKV- 797
            ++Q+ G  + S C LC    ET  HLF+ CCF+ ++W W A   ++S H   + D   V 
Sbjct: 142  KLQQRGCQLPSMCSLCGTHVETSFHLFFECCFAFRIWCWFASVLDSSLHFQSIPDIWSVC 201

Query: 796  NSQWSPRLKQLWHTCIVAMLTSIWKQRN 713
            +  WSP+ K +    IV ++++IW  RN
Sbjct: 202  DRGWSPQCKVVNQATIVNIISTIWYNRN 229



 Score = 74.3 bits (181), Expect = 6e-11
 Identities = 52/152 (34%), Positives = 71/152 (46%), Gaps = 6/152 (3%)
 Frame = -3

Query: 579 WDVASKIRNAPTVKQCI---WIPPAPNILKLNTDGASLGNPRPAGIGISYRDNMGGFKLT 409
           W    K+ N  T+   I   W       +K NTDGAS       G G+ +RD    F   
Sbjct: 205 WSPQCKVVNQATIVNIISTIWYNRNQRWIKFNTDGASTATSSSCG-GV-FRDQNANFLSC 262

Query: 408 VWKSIGVGTNFWAECMAILEGVEIAMSKGWYKL*IETDSEAAAQVFRS-GNVPWTLQTRW 232
             K++G G+ +  E  AI+  VEIA  + W  + IE+DS      F+    +P  L+ RW
Sbjct: 263 FAKNLGGGSAYQPELAAIMRVVEIAYQRNWRNIWIESDSALVVMAFKKMSMIPCRLRNRW 322

Query: 231 NNC--TLAVPDIKITSIFREANYTADRLAKIG 142
            NC   L   +  IT I+RE N  AD+LA  G
Sbjct: 323 INCRKLLCRMNFVITHIYREDNRCADKLASKG 354