BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00027150
         (1559 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|2...   615   e-174
ref|XP_002532945.1| Pectinesterase-3 precursor, putative [Ricinu...   611   e-172
ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]      595   e-168
gb|AFI23414.1| pectin methylesterase [Coffea arabica]                 570   e-160
ref|XP_004144445.1| PREDICTED: pectinesterase 3-like [Cucumis sa...   567   e-159

>ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|222841688|gb|EEE79235.1|
            predicted protein [Populus trichocarpa]
          Length = 588

 Score =  615 bits (1587), Expect = e-174
 Identities = 294/484 (60%), Positives = 375/484 (77%), Gaps = 7/484 (1%)
 Frame = -1

Query: 1559 LFKLSMQIAINEISRISSLPDQFISNLNDTLAEDALRNCKELFDDSIDQLNTSISSMTVG 1380
            LFKLS+++A+NE+S++   PD+ I ++ DT  + AL+ C  +FDD++D+LN SISSM +G
Sbjct: 106  LFKLSLRVAMNELSKLKDYPDKLIQSIKDTTLQGALKVCATVFDDAVDRLNDSISSMAIG 165

Query: 1379 GGESLSSQQAQMDDIKTWISTAITDQETCLDGLNETTKSDVFNE-----MKMAMKNSTEF 1215
             GE + S  A+M+D+KTW+S  ITDQETCLD L E + +  FN+     +K AM+NSTEF
Sbjct: 166  EGEQILSP-AKMNDLKTWLSATITDQETCLDALQELSTTKHFNQTLVDQVKTAMENSTEF 224

Query: 1214 TSNSLAIVTKVMSVIDHLNIPVHRKLLGYIDHHGSDFPSWLHPMDRRLLQVANPKADITV 1035
             SNSLAIV K++ ++    IP+HR+LLG+   H S+FP W+   DRRLLQ + P  ++TV
Sbjct: 225  VSNSLAIVAKILGLLSDFKIPIHRRLLGFERSHISEFPQWVSFGDRRLLQESKPTPNVTV 284

Query: 1034 AKDGSGNFRTITAAIKAVGRKNKTRTVIYVKQGEYVENVVLDKTCWNIMMYGDGKAKTVV 855
            AKDGSG+  T+  A+  + +K++++ +I+VK+G YVENV+LDK+ WN+M+YG+GK KT+V
Sbjct: 285  AKDGSGDCETLREAVGKIPKKSESKFIIHVKEGVYVENVILDKSKWNVMIYGEGKDKTIV 344

Query: 854  SGSSNVVDGTPTFSTAPFIVVGKGFIAKDMGFVNTAGAQKHQAVALRSGSDQSVFYQCSF 675
            SGS N VDGTPTFST  F V GKGF A+DM F+NTAGA+KHQAVA RSGSD SVFY+C+F
Sbjct: 345  SGSLNFVDGTPTFSTPTFAVAGKGFFARDMKFINTAGAEKHQAVAFRSGSDMSVFYRCAF 404

Query: 674  GAYQDTLYAHSNRQFYRECDITGTVDFIFGNAAVIFQNCNIQPRQPLPNQFNTLTAQGKI 495
             A+QDTLYAHSNRQFYR+CDITGT+DFIFGNAAV+FQNCNIQPRQPL NQFNT+TAQGK 
Sbjct: 405  DAFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIQPRQPLANQFNTITAQGKK 464

Query: 494  DPNQNTGISIQGCTIAALGNVTVNTYLGRPWKNFSTTIIMKSVIGSVVSPPGWTPWVTNI 315
            DPNQNTGISIQ C  +A  NVT  TYLGRPWK++STT+IM+S IGS + P GW  WV+ +
Sbjct: 465  DPNQNTGISIQKCKFSAFDNVTAPTYLGRPWKDYSTTVIMQSDIGSFLRPLGWISWVSGV 524

Query: 314  KPASTIFYAEYQNTGPGSSVAQRVTWA--KPTATADEAAKFTVEPFIQGSQWLPQAGVTF 141
             P +TIFYAEYQNTG G++V  RV W   KP  T DEA KF V+ FIQGS+WLP+  VTF
Sbjct: 525  DPPATIFYAEYQNTGSGANVDGRVKWTGYKPALTVDEAGKFAVDSFIQGSEWLPKTSVTF 584

Query: 140  TSAL 129
             S L
Sbjct: 585  QSML 588


>ref|XP_002532945.1| Pectinesterase-3 precursor, putative [Ricinus communis]
            gi|223527274|gb|EEF29429.1| Pectinesterase-3 precursor,
            putative [Ricinus communis]
          Length = 589

 Score =  611 bits (1576), Expect = e-172
 Identities = 300/486 (61%), Positives = 375/486 (77%), Gaps = 9/486 (1%)
 Frame = -1

Query: 1559 LFKLSMQIAINEISRISSLPDQFISNLN-DTLAEDALRNCKELFDDSIDQLNTSISSMTV 1383
            LFKLS+ + INE+S+I   P + I N N D   + AL  C+ +FDD+ D LN SISSMTV
Sbjct: 106  LFKLSLHVVINELSKIKDYPSKLIQNSNLDATVKAALNVCESVFDDAFDMLNDSISSMTV 165

Query: 1382 GGGESLSSQQAQMDDIKTWISTAITDQETCLDGLNETT-----KSDVFNEMKMAMKNSTE 1218
            G GE++ S  ++++D+KTW+ST ITDQETCLD L E        S V  +++ AM+NSTE
Sbjct: 166  GEGETILSP-SKINDMKTWLSTTITDQETCLDALQELNGTKHFDSKVLEDIRTAMENSTE 224

Query: 1217 FTSNSLAIVTKVMSVIDHLNIPVHRKLLGYIDHHGSDFPSWLHPMDRRLLQVANPKADIT 1038
            F SNSLAIV K++ ++   NIP+HRKLLG+ +   S FPSW+   DRRLLQ A P A++T
Sbjct: 225  FASNSLAIVAKIIGLLTDFNIPIHRKLLGF-ERTSSGFPSWVSIGDRRLLQEAKPAANVT 283

Query: 1037 VAKDGSGNFRTITAAIKAVGRKNKTRTVIYVKQGEYVENVVLDKTCWNIMMYGDGKAKTV 858
            VAKDGSG++ TI  A+ A+ +K+ +R +I+VK+G YVEN+++DK  WN+M+YGDGK KT+
Sbjct: 284  VAKDGSGDYTTIGEAVDAIPKKSPSRFIIHVKEGTYVENILMDKHKWNVMIYGDGKDKTI 343

Query: 857  VSGSSNVVDGTPTFSTAPFIVVGKGFIAKDMGFVNTAGAQKHQAVALRSGSDQSVFYQCS 678
            +SGS+N VDGTPTFSTA F V GKGF+A+D+ F+NTAGA KHQAVA RSGSD SV++QCS
Sbjct: 344  ISGSTNFVDGTPTFSTATFAVAGKGFMARDIKFINTAGAAKHQAVAFRSGSDMSVYFQCS 403

Query: 677  FGAYQDTLYAHSNRQFYRECDITGTVDFIFGNAAVIFQNCNIQPRQPLPNQFNTLTAQGK 498
            F AYQDTLYAHSNRQFYR+CDITGT+DFIFGNAAV+FQNCNI PRQPLPNQFNT+TAQGK
Sbjct: 404  FDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIMPRQPLPNQFNTITAQGK 463

Query: 497  IDPNQNTGISIQGCTIAALG-NVTVNTYLGRPWKNFSTTIIMKSVIGSVVSPPGWTPWVT 321
             DPNQNTGISIQ C   A G N+T +TYLGRPWKNFSTT+IM+S I S ++P GW  WV+
Sbjct: 464  KDPNQNTGISIQKCKFYAFGPNLTASTYLGRPWKNFSTTVIMQSNIDSFLNPLGWMSWVS 523

Query: 320  NIKPASTIFYAEYQNTGPGSSVAQRVTWA--KPTATADEAAKFTVEPFIQGSQWLPQAGV 147
             I P S+IFY EYQNTGPGS++ +R+ W   KP+ T  EA KF+V  FIQG+ WLP   V
Sbjct: 524  GIDPPSSIFYGEYQNTGPGSAIEKRIQWVGYKPSLTEVEAGKFSVGSFIQGTDWLPATSV 583

Query: 146  TFTSAL 129
            TF S+L
Sbjct: 584  TFDSSL 589


>ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]
          Length = 578

 Score =  595 bits (1535), Expect = e-168
 Identities = 296/479 (61%), Positives = 369/479 (77%), Gaps = 2/479 (0%)
 Frame = -1

Query: 1559 LFKLSMQIAINEISRISSLPDQFISNLNDTLAEDALRNCKELFDDSIDQLNTSISSMTVG 1380
            LF+L++++AI E+S++SSLP Q  +  ND   + AL  C+ +F+D+ID+LN SISSM V 
Sbjct: 104  LFRLTLRVAIAELSKLSSLPRQLSAKSNDAQLKKALGVCETVFEDAIDRLNDSISSMEVR 163

Query: 1379 GGESLSSQQAQMDDIKTWISTAITDQETCLDGLNETTKSDVFNEMKMAMKNSTEFTSNSL 1200
             GE L S  +++DDIKTW+S  ITDQETCLD L E   S + NE+K AM+NST F SNSL
Sbjct: 164  EGEKLLSA-SKIDDIKTWLSATITDQETCLDALEELN-STLLNEVKTAMQNSTVFASNSL 221

Query: 1199 AIVTKVMSVIDHLNIPVHRKLLGYIDHHGSDFPSWLHPMDRRLLQVANPKADITVAKDGS 1020
            AIV K++ ++  L+I VHRKLL +   +   FP W+   +RRLLQ   P  D+TVAKDG+
Sbjct: 222  AIVAKLIGILHDLDIQVHRKLLSF--SNSDQFPDWVGAGERRLLQETKPTPDVTVAKDGT 279

Query: 1019 GNFRTITAAIKAVGRKNKTRTVIYVKQGEYVENVVLDKTCWNIMMYGDGKAKTVVSGSSN 840
            G++ TI  A+  V +K++ R VIYVK+G Y EN++LDK+ WN+M+YGDGK K++VSG+ N
Sbjct: 280  GDYVTIKEAVAMVPKKSEKRFVIYVKEGNYSENIILDKSKWNVMIYGDGKDKSIVSGNLN 339

Query: 839  VVDGTPTFSTAPFIVVGKGFIAKDMGFVNTAGAQKHQAVALRSGSDQSVFYQCSFGAYQD 660
             +DGTPTF+TA F  VGKGFIAK M F NTAGA KHQAVA RSGSD SVFYQCSF A+QD
Sbjct: 340  FIDGTPTFATATFAAVGKGFIAKYMRFENTAGAAKHQAVAFRSGSDMSVFYQCSFDAFQD 399

Query: 659  TLYAHSNRQFYRECDITGTVDFIFGNAAVIFQNCNIQPRQPLPNQFNTLTAQGKIDPNQN 480
            TLYAHSNRQFYRECDITGT+DFIFGNAAV+FQ C IQPRQP+ NQFNT+TAQGK DPNQN
Sbjct: 400  TLYAHSNRQFYRECDITGTIDFIFGNAAVVFQACKIQPRQPMSNQFNTITAQGKKDPNQN 459

Query: 479  TGISIQGCTIAALGNVTVNTYLGRPWKNFSTTIIMKSVIGSVVSPPGWTPWVTNIKPAST 300
            TGISIQ C+I+AL  +T  TYLGRPWK +STTI+M+S IGS ++P GWT WVT + P ST
Sbjct: 460  TGISIQKCSISALNTLTAPTYLGRPWKAYSTTIVMQSNIGSFLNPKGWTEWVTGVDPPST 519

Query: 299  IFYAEYQNTGPGSSVAQRVTWA--KPTATADEAAKFTVEPFIQGSQWLPQAGVTFTSAL 129
            IFYAE+QNTGPG+++ QRV WA      T DEAAKFTV  FIQG+ WL ++ VTF ++L
Sbjct: 520  IFYAEFQNTGPGATLDQRVKWAGFMTNITEDEAAKFTVGTFIQGASWLSESSVTFDASL 578


>gb|AFI23414.1| pectin methylesterase [Coffea arabica]
          Length = 582

 Score =  570 bits (1468), Expect = e-160
 Identities = 275/478 (57%), Positives = 355/478 (74%), Gaps = 4/478 (0%)
 Frame = -1

Query: 1559 LFKLSMQIAINEISRISSLPDQFISNLNDTLAEDALRNCKELFDDSIDQLNTSISSMTVG 1380
            LF+LS+Q+    + ++S+LP  +IS+  D   + AL  C+ + DD++D  + S+SS+ V 
Sbjct: 106  LFQLSLQVVHASLQKLSTLPQHWISDARDLPLKKALGVCQAVIDDAVDATDESLSSLNVS 165

Query: 1379 GGESLSSQQAQMDDIKTWISTAITDQETCLDGLNETTKSDVFNEMKMAMKNSTEFTSNSL 1200
             G+ L +   +++D+KTW+S ++TD ETCLD L E   + +  +++ + +NSTEF SNSL
Sbjct: 166  EGDRLLTVD-RVNDLKTWLSASLTDLETCLDSLQEVNATVLAEQVRASSRNSTEFASNSL 224

Query: 1199 AIVTKVMSVIDHLNIPVHRKLLGYIDHHGSDFPSWLHPMDRRLLQVANP--KADITVAKD 1026
            AIV+K+++++   NIP+HRKLL         FP W+   DRRLLQ  N   K D+ VA+D
Sbjct: 225  AIVSKLLTILSGFNIPIHRKLLAAGTDSDGGFPRWVRAADRRLLQTPNENTKPDLVVAQD 284

Query: 1025 GSGNFRTITAAIKAVGRKNKTRTVIYVKQGEYVENVVLDKTCWNIMMYGDGKAKTVVSGS 846
            GSG++RTI+ A+  + +K+KTR VIYVK G Y E V LDK+ WN+MMYGDGKAKT+V+  
Sbjct: 285  GSGDYRTISEAVAKIPKKSKTRFVIYVKAGVYKEKVSLDKSTWNVMMYGDGKAKTIVTSD 344

Query: 845  SNVVDGTPTFSTAPFIVVGKGFIAKDMGFVNTAGAQKHQAVALRSGSDQSVFYQCSFGAY 666
             N VDGTPTF TA F V GKGFIAK M F NTAGA KHQAVA RSGSDQSV Y CSF A+
Sbjct: 345  DNFVDGTPTFDTATFAVAGKGFIAKSMAFRNTAGAAKHQAVAFRSGSDQSVLYLCSFDAF 404

Query: 665  QDTLYAHSNRQFYRECDITGTVDFIFGNAAVIFQNCNIQPRQPLPNQFNTLTAQGKIDPN 486
            QDTLY HSNRQFYRECDI+GT+DFIFGNAAV+FQNCNI+PRQPLPNQF T+TAQGK DPN
Sbjct: 405  QDTLYPHSNRQFYRECDISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFVTITAQGKKDPN 464

Query: 485  QNTGISIQGCTIAALGNVTVNTYLGRPWKNFSTTIIMKSVIGSVVSPPGWTPWVTNIKPA 306
            QNTGI+IQ C ++ L  +T  TYLGRPWK +STT+IM++ IG+ ++P GW  WV N++P 
Sbjct: 465  QNTGITIQNCVMSPLDKLTAPTYLGRPWKPYSTTVIMQTNIGAFLAPKGWIEWVFNVEPP 524

Query: 305  STIFYAEYQNTGPGSSVAQRVTW--AKPTATADEAAKFTVEPFIQGSQWLPQAGVTFT 138
            STIFY EYQNTGPGSSVAQRV W    P+ TA +A+K+TV+ FI G  W+P + VTFT
Sbjct: 525  STIFYGEYQNTGPGSSVAQRVKWDGLNPSLTATQASKYTVKSFIAGQSWIPASAVTFT 582


>ref|XP_004144445.1| PREDICTED: pectinesterase 3-like [Cucumis sativus]
            gi|449528768|ref|XP_004171375.1| PREDICTED:
            pectinesterase 3-like [Cucumis sativus]
          Length = 576

 Score =  567 bits (1461), Expect = e-159
 Identities = 276/480 (57%), Positives = 349/480 (72%), Gaps = 3/480 (0%)
 Frame = -1

Query: 1559 LFKLSMQIAINEISRISSLPDQFISNLNDTLAEDALRNCKELFDDSIDQLNTSISSMTVG 1380
            LFKLS+++A + +S++S     F S   D   E A++ C+ +F+D+ID LN ++SSM V 
Sbjct: 105  LFKLSLRVATDSLSKLSDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVD 164

Query: 1379 GGESLSSQQAQMDDIKTWISTAITDQETCLDGLNETTKSDVFNEMKMAMKNSTEFTSNSL 1200
                     ++++D+KTW+ST ITDQETCLD L +  ++ V  +++ AM NSTEFTSNSL
Sbjct: 165  RHSEKFLSPSRIEDLKTWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSL 224

Query: 1199 AIVTKVMSVIDHLNIPVHRKLLGYIDHHGSDFPSWLHPMDRRLLQVANPKADITVAKDGS 1020
            AIVTK++ ++   NIP+HRKL+G         P W+   DRRLLQ  N  A +TV+KDG 
Sbjct: 225  AIVTKILGLLADFNIPIHRKLMG--------LPEWVSSGDRRLLQENNVTAHVTVSKDGK 276

Query: 1019 GNFRTITAAIKAVGRKNKTRTVIYVKQGEYVENVVLDKTCWNIMMYGDGKAKTVVSGSSN 840
            G + TI  A+ AV +K+K R +I+VK+G Y ENV+LDK+ WN+MMYGDG+ KT+VSG  N
Sbjct: 277  GQYTTIQDAVAAVPKKSKERFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLN 336

Query: 839  VVDGTPTFSTAPFIVVGKGFIAKDMGFVNTAGAQKHQAVALRSGSDQSVFYQCSFGAYQD 660
             +DGTPTFSTA F V GKGFI KDMGF+NTAG  KHQAVA RSGSD SV   CSF  YQD
Sbjct: 337  FIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQD 396

Query: 659  TLYAHSNRQFYRECDITGTVDFIFGNAAVIFQNCNIQPRQPLPNQFNTLTAQGKIDPNQN 480
            TLYAHSNRQFYR+CDITGT+DFIFGNAAV+FQNCNI+PRQPLPNQFNT+TAQGK D NQN
Sbjct: 397  TLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQN 456

Query: 479  TGISIQGCTIAALG-NVTVNTYLGRPWKNFSTTIIMKSVIGSVVSPPGWTPWVTNIKPAS 303
            +GISIQ CT +A   ++   TYLGRPWK FSTT+IM+S IG  ++P GW  WV+   P S
Sbjct: 457  SGISIQKCTFSAYNDSLNAPTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPS 516

Query: 302  TIFYAEYQNTGPGSSVAQRVTWA--KPTATADEAAKFTVEPFIQGSQWLPQAGVTFTSAL 129
            +IFY EYQN+GPGS+V +RV WA  KP+ T  EA +FTV  F+ G  WLP   V F ++L
Sbjct: 517  SIFYGEYQNSGPGSNVDKRVRWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576