BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00027107
         (1642 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002327186.1| cytochrome P450 [Populus trichocarpa] gi|222...   439   e-120
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   439   e-120
ref|XP_002327188.1| cytochrome P450 [Populus trichocarpa] gi|222...   434   e-119
ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis ...   432   e-118
dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       432   e-118

>ref|XP_002327186.1| cytochrome P450 [Populus trichocarpa] gi|222835501|gb|EEE73936.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 506

 Score =  439 bits (1129), Expect = e-120
 Identities = 233/501 (46%), Positives = 321/501 (64%), Gaps = 4/501 (0%)
 Frame = +3

Query: 45   LVIFFLSKKVL-HIKHHPKNLPPSPRSLPVIGHFHLLGKPIHIAVDSLLRQYGQILFLWF 221
            L  FFL   V+ ++ H  KNLPPSP SLP+IGH HLL  P+H  + +LL+QYG +L L  
Sbjct: 7    LAFFFLIFLVIKYVFHGNKNLPPSPPSLPIIGHLHLLKPPLHQTLQTLLQQYGPVLSLKA 66

Query: 222  GSRPVLVLCSPTAIKECFTRKDIIFASRPRLILGEILS-KYSAIPMAPYGDLWRNLRRFT 398
            G R +LVL SP+A++ECFT+ D++ ++RP  + G+ L+  Y+ I  +PYG LWR LRRF 
Sbjct: 67   GCRSMLVLSSPSAVEECFTKNDVVLSNRPTFLAGDHLTYNYTTIIFSPYGHLWRTLRRFA 126

Query: 399  ASEIFSTNCLDISSSIRREEVWSVIRGMCK-SCDGEFENVELNSIFKKLIFNILMRILGM 575
              E+FS   L+  S++R+EEV S++R + K SC G  + V+L+  F  L FN+ MR+   
Sbjct: 127  VLEMFSQKGLNKFSAVRKEEVCSLLRQLSKVSCSGN-KKVDLHYFFSLLSFNVAMRMSAG 185

Query: 576  KPCFGEEEMVDPEKAEDKLDELRSRFFPKQVFAAGDFFPFMKWIDHWRIKSSSSEFFTKR 755
            K C  EEE+   +  +  L EL+  F P       DFFP +KWID+   + S  +    R
Sbjct: 186  KKCI-EEEVACSDLGKQDLTELKKIFHPPLSTGLCDFFPALKWIDYKGFEKSVIKVRDGR 244

Query: 756  DKYMQDIIDDLRFKRXXXXXXFNTNTTREVTKDEKESKRTIIEVLLSLQEAEPECYTDDI 935
            D + QD+ID++R K+          T+   + D    K T+IE LLSLQE EP+ YTDDI
Sbjct: 245  DGFSQDLIDEIRQKK----------TSSCSSPDAGPEKTTMIETLLSLQEQEPDFYTDDI 294

Query: 936  IKGIILVVLNAGTETSTTTMEWAMSFLLNHPEVLNKARNEIDSIVGYDRLLNESDFGKLP 1115
            IKG+++ + +AGT+T   TMEWAM+ LLNHPE+L K R EIDS VG+ RL+ E D  KL 
Sbjct: 295  IKGLVVAMFSAGTDTVAVTMEWAMALLLNHPEILQKVRVEIDSQVGHTRLVEEVDLPKLK 354

Query: 1116 YLHSIISETLRLRPIGPFIPPHESMEDCTIGGFDIPKGTMLLVNQLALHSDPNVWPEPMK 1295
            YL  +I+ETLRL P+ P + P    EDCT+ G+++PKGT+LLVN  A+H DP +W +P +
Sbjct: 355  YLRCVINETLRLYPVVPLLLPRCPSEDCTVAGYNVPKGTILLVNAFAMHRDPKMWEQPDR 414

Query: 1296 FKPDRFEKGNGE-NGVNWIAFGLGRRACPGEGLALRMLGLTLGVLIQFFDWERIGGDMVD 1472
            FKP+RFE    E  G+ +I FG+GRRACPG  + +R + L +  L Q F+WER G +MVD
Sbjct: 415  FKPERFEATVEEKEGIKFIPFGMGRRACPGSNMGMRAIMLAMAALFQCFEWERTGQEMVD 474

Query: 1473 MSVREQGIRAKAKPLEAMYRP 1535
            M+V       KAKPLEA  +P
Sbjct: 475  MTVAAAISMVKAKPLEAFCKP 495


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  439 bits (1128), Expect = e-120
 Identities = 233/503 (46%), Positives = 326/503 (64%), Gaps = 3/503 (0%)
 Frame = +3

Query: 39   LVLVIFFLSKKVLHIKHHPKNLPPSPRSLPVIGHFHLLGKPIHIAVDSLLRQYGQILFLW 218
            L+ + F L  K++      KNLPPSP SLP+IGH HL  KP++  + ++  QYG +L+  
Sbjct: 9    LLFITFLLLSKLVFSSPKHKNLPPSPPSLPIIGHLHLFKKPLYRTLTNISNQYGPVLYFQ 68

Query: 219  FGSRPVLVLCSPTAIKECFTRKDIIFASRPRLILGEIL-SKYSAIPMAPYGDLWRNLRRF 395
            FGSRPVLV+ SP+A +EC T+ DI FA+RPRL++G+   + Y+ +  A YG  WRNLRR 
Sbjct: 69   FGSRPVLVVSSPSAAEECLTKNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRI 128

Query: 396  TASEIFSTNCLDISSSIRREEVWSVIRGMCKSCDGEFENVELNSIFKKLIFNILMRILGM 575
            +A E+FS N + + S IR +EV S++R +      E+  VE+ S+F +L  NI+MR++  
Sbjct: 129  SALEMFSPNRIQMFSEIRTDEVKSMVRRLVAGY--EYHKVEMKSVFFELTLNIMMRMIAG 186

Query: 576  KPCFGEEEMVDPEKAEDKLDELRSRFFPKQVFAAGDFFPFMKWIDHWRIKSSSSEFFTKR 755
            K  +GE  + D E+A    + +   F        GDF P ++WI +  I+        KR
Sbjct: 187  KRYYGEH-VADLEQARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKR 245

Query: 756  DKYMQDIIDDLRFKRXXXXXXFNTNTTREVTKDEKESKRTIIEVLLSLQEAEPECYTDDI 935
            D+++Q++I + R  R           ++    +EK  K+T+I+VLLSLQE EPE YTD++
Sbjct: 246  DEFLQELIQERRKLRV---------NSKSFPSEEK--KKTLIDVLLSLQETEPEYYTDEM 294

Query: 936  IKGIILVVLNAGTETSTTTMEWAMSFLLNHPEVLNKARNEIDSIVGYDRLLNESDFGKLP 1115
            I+G+I ++  AGT+TS  TMEWAMS LLN+P+++ KA++EID  +   R ++ESD  KLP
Sbjct: 295  IRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLP 354

Query: 1116 YLHSIISETLRLRPIGPFIPPHESMEDCTIGGFDIPKGTMLLVNQLALHSDPNVWPEPMK 1295
            YLH II+ETLR+ P GP + PHES EDC IGG++IP GTMLLVN  A+ +DPN+W EP K
Sbjct: 355  YLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRK 414

Query: 1296 FKPDRFE--KGNGENGVNWIAFGLGRRACPGEGLALRMLGLTLGVLIQFFDWERIGGDMV 1469
            FKP+RFE  +G   +G   + FG GRR CPGEGLA R++GLTL  L+Q FDW R+G +MV
Sbjct: 415  FKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMV 474

Query: 1470 DMSVREQGIRAKAKPLEAMYRPR 1538
            +MS        K  PLEA  RPR
Sbjct: 475  EMSEGTGLTLPKLHPLEAHCRPR 497


>ref|XP_002327188.1| cytochrome P450 [Populus trichocarpa] gi|222835503|gb|EEE73938.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 510

 Score =  434 bits (1115), Expect = e-119
 Identities = 229/500 (45%), Positives = 327/500 (65%), Gaps = 2/500 (0%)
 Frame = +3

Query: 48   VIFFLSKKVLHIKHHPKNLPPSPRSLPVIGHFHLLGKPIHIAVDSLLRQYGQILFLWFGS 227
            ++FFL   V ++ H  +NLPP+P +LPVIGH +LL +P++ ++ +LL +YG  L L FGS
Sbjct: 10   LLFFLFIVVKNLFHRKRNLPPAPFALPVIGHLYLLKQPLYKSLHALLSRYGPALSLRFGS 69

Query: 228  RPVLVLCSPTAIKECFTRKDIIFASRPRLILGEILS-KYSAIPMAPYGDLWRNLRRFTAS 404
            R V+V+ SP+ ++ECFT+ D IFA+RP+ + G+ L+  YSA   APYGDLWR LRR   +
Sbjct: 70   RFVIVVSSPSVVEECFTKNDKIFANRPKSMAGDRLTYNYSAFVWAPYGDLWRKLRRLAVA 129

Query: 405  EIFSTNCLDISSSIRREEVWSVIRGMCKSCDGEFENVELNSIFKKLIFNILMRILGMKPC 584
            EIFS+  L  SS++R EEV  +IR + K      +NVEL  +F  L  N++M +   K C
Sbjct: 130  EIFSSKSLRKSSTVREEEVSCLIRRLLKVSTSGTQNVELRLLFSILASNVVMIVSAGKRC 189

Query: 585  FGEEEMVDPEKAEDKLDELRSRFFPKQVFAAGDFFPFMKWIDHWRIKSSSSEFFTKRDKY 764
              EEE    +  +    + + +FFP       DF P ++ I    ++ +  +    RD++
Sbjct: 190  V-EEEHAGTKMEKQLFQDFKDKFFPSLAMNICDFIPILRVIGFKGLEKNMKKLHGIRDEF 248

Query: 765  MQDIIDDLRFKRXXXXXXFNTNTTREVTKDEKESKRTIIEVLLSLQEAEPECYTDDIIKG 944
            +Q++ID++R K         +  T EVT  + E +R++ E+LL LQE+EPE YTD++IK 
Sbjct: 249  LQNLIDEIRLKLKKT----TSLKTDEVT--DGEERRSVAEILLCLQESEPEFYTDEVIKS 302

Query: 945  IILVVLNAGTETSTTTMEWAMSFLLNHPEVLNKARNEIDSIVGYDRLLNESDFGKLPYLH 1124
             +L++  AGTETS  T+EWAM+ LLNHP+V+ K + EID  VG+ RLLNESD  KLPYL 
Sbjct: 303  TVLMMFIAGTETSAITLEWAMTLLLNHPKVMQKVKAEIDEHVGHGRLLNESDIVKLPYLR 362

Query: 1125 SIISETLRLRPIGPFIPPHESMEDCTIGGFDIPKGTMLLVNQLALHSDPNVWPEPMKFKP 1304
             +I+ETLRL P  P + PH S E CT GGFDIP+GTML+VN   +H DP +W EP +FKP
Sbjct: 363  CVINETLRLYPPAPLLLPHFSSEACTAGGFDIPQGTMLVVNAWTMHRDPKLWEEPNEFKP 422

Query: 1305 DRFEKGNGE-NGVNWIAFGLGRRACPGEGLALRMLGLTLGVLIQFFDWERIGGDMVDMSV 1481
            +RFE G GE +G  +I FG+GRR CPG  + L+++ L LGVL+Q F+W+++ G + D S 
Sbjct: 423  ERFEAGLGEGDGFKYIPFGIGRRVCPGASMGLQIVSLALGVLVQCFEWDKV-GTVEDTSH 481

Query: 1482 REQGIRAKAKPLEAMYRPRK 1541
                I +KAKPLEA+  PR+
Sbjct: 482  GLGMILSKAKPLEALCSPRR 501


>ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis vinifera]
          Length = 507

 Score =  432 bits (1112), Expect = e-118
 Identities = 230/512 (44%), Positives = 324/512 (63%), Gaps = 5/512 (0%)
 Frame = +3

Query: 18   GNMEIVALVLV-IFFLSKKVLHIKHHPK-NLPPSPRSLPVIGHFHLLGKPIHIAVDSLLR 191
            GN+   A++L+ I  + K + H +   +  LPPSP +LPVIGH HLL  P++  + +L  
Sbjct: 2    GNLYHYAVILLPIILIIKFLFHGRQRQRYRLPPSPFALPVIGHLHLLKPPLYQGLQALSS 61

Query: 192  QYGQILFLWFGSRPVLVLCSPTAIKECFTRKDIIFASRPRLILGE-ILSKYSAIPMAPYG 368
            QYG ILFL FG RP +V+ SP+A++ECFT+ D++ A+RPR ++G+ +   Y+A   A YG
Sbjct: 62   QYGPILFLRFGCRPFVVVSSPSAVQECFTKNDVVLANRPRSMIGDHVTYNYTAFAWASYG 121

Query: 369  DLWRNLRRFTASEIFSTNCLDISSSIRREEVWSVIRGMCKSCDGEFENVELNSIFKKLIF 548
             LWR LRR T  EI S+N L + S++R EEV  ++R + K  +   + V++        F
Sbjct: 122  HLWRVLRRLTVVEILSSNKLLLLSTVREEEVRYLLRQLFKVSNDGAQKVDMRLYLSLFSF 181

Query: 549  NILMRILGMKPCFGEEEMVDPEKAEDKLDELRSRFFPKQVFAAGDFFPFMKWIDHWRIKS 728
            N +M+ +  K C  EEE    E     L+ L+  F P       DFFP ++W+ +  ++ 
Sbjct: 182  NFIMKTITGKRCI-EEEAEGIETNRQFLERLKRIFVPTTTTNLCDFFPILRWVGYKGLEK 240

Query: 729  SSSEFFTKRDKYMQDIIDDLRFKRXXXXXXFNTNTTREVTKDEKESKRTIIEVLLSLQEA 908
            S  +F  +RD Y+Q ++D+ R               R  +  E + KRT+IE LL LQ++
Sbjct: 241  SVIQFGKERDGYLQGMLDEFR---------------RNNSAVEWQKKRTLIETLLFLQQS 285

Query: 909  EPECYTDDIIKGIILVVLNAGTETSTTTMEWAMSFLLNHPEVLNKARNEIDSIVGYDRLL 1088
            EP+ YTDD+IKG++LVV++AGT+TS+ T+EWAMS LLNHPE L KAR EIDS V    LL
Sbjct: 286  EPDFYTDDVIKGLMLVVISAGTDTSSVTLEWAMSLLLNHPEALEKARAEIDSHVKPGHLL 345

Query: 1089 NESDFGKLPYLHSIISETLRLRPIGPFIPPHESMEDCTIGGFDIPKGTMLLVNQLALHSD 1268
            ++SD  KLPYL S+++ETLRL P  P + PH S EDC++GGFDIP+GT ++VN  ALH D
Sbjct: 346  DDSDLAKLPYLRSVVNETLRLYPTAPLLLPHLSSEDCSVGGFDIPRGTTVMVNVWALHRD 405

Query: 1269 PNVWPEPMKFKPDRFE--KGNGENGVNWIAFGLGRRACPGEGLALRMLGLTLGVLIQFFD 1442
            P VW E  KFKP+RFE  +   +    +  FG+GRRACPG  LA++++ L LG LIQ F+
Sbjct: 406  PRVWEEATKFKPERFEGMENEEKEAFKFAPFGIGRRACPGAALAMKIVSLALGGLIQCFE 465

Query: 1443 WERIGGDMVDMSVREQGIRAKAKPLEAMYRPR 1538
            WER+  + VDMS        KAKPLE ++RPR
Sbjct: 466  WERVEAEKVDMSPGSGITMPKAKPLEIIFRPR 497


>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  432 bits (1112), Expect = e-118
 Identities = 233/504 (46%), Positives = 318/504 (63%), Gaps = 2/504 (0%)
 Frame = +3

Query: 33   VALVLVIFFLSKKVLHIKHHPKNLPPSPRSLPVIGHFHLLGKPIHIAVDSLLRQYGQILF 212
            ++ +LVI FL  K        KNLPP P SLP+IGH HL  KP+H  +  L  +YG +L+
Sbjct: 11   ISSILVIKFLVPK------RYKNLPPGPPSLPLIGHLHLFKKPLHRTLAKLSDKYGHVLY 64

Query: 213  LWFGSRPVLVLCSPTAIKECFTRKDIIFASRPRLILGEILSK-YSAIPMAPYGDLWRNLR 389
            L FGSRPVLV+ SP+A ++C  + DIIFA+RP L++G+IL   Y+ +  APYG  WRNLR
Sbjct: 65   LQFGSRPVLVVSSPSAAEDCLAKNDIIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLR 124

Query: 390  RFTASEIFSTNCLDISSSIRREEVWSVIRGMCKSCDGEFENVELNSIFKKLIFNILMRIL 569
            + +A E+ S N L   S IR +EV S+++ +      E+  VE+ ++  +L  N++M ++
Sbjct: 125  KISAIELLSANRLQSYSDIRHDEVKSMVQRLSGQ-GNEYRTVEMKTMIFELTLNVMMTMI 183

Query: 570  GMKPCFGEEEMVDPEKAEDKLDELRSRFFPKQVFAAGDFFPFMKWIDHWRIKSSSSEFFT 749
              K  +G + + + E+A+   + ++           GDF P + WI    I+        
Sbjct: 184  AGKRYYGGQ-VEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLKE 242

Query: 750  KRDKYMQDIIDDLRFKRXXXXXXFNTNTTREVTKDEKESKRTIIEVLLSLQEAEPECYTD 929
            KRDK++Q++I++ R  R                   KE   T+I+VLLSLQE EPE YTD
Sbjct: 243  KRDKFLQELIEEHRISRSGGM-------------QTKEKNATLIDVLLSLQETEPEYYTD 289

Query: 930  DIIKGIILVVLNAGTETSTTTMEWAMSFLLNHPEVLNKARNEIDSIVGYDRLLNESDFGK 1109
            ++I GII V+L AGT+TS  TMEWAMS LLN+P+V+ KA+ EID+ +   RL+NESD  K
Sbjct: 290  EMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLINESDVNK 349

Query: 1110 LPYLHSIISETLRLRPIGPFIPPHESMEDCTIGGFDIPKGTMLLVNQLALHSDPNVWPEP 1289
            LPYLHSII+ETLR+ P GP + PHES E+C +GG+ +P GTMLLVN  A+  DPN+W EP
Sbjct: 350  LPYLHSIITETLRIYPAGPLLVPHESSEECIVGGYKVPSGTMLLVNVWAIQQDPNIWVEP 409

Query: 1290 MKFKPDRFEKGNG-ENGVNWIAFGLGRRACPGEGLALRMLGLTLGVLIQFFDWERIGGDM 1466
             KFKP+RF+   G  +G   + FG GRR CPGEGLA+R++ L LG LIQ FDWER+G +M
Sbjct: 410  TKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAMRVVALALGALIQCFDWERVGEEM 469

Query: 1467 VDMSVREQGIRAKAKPLEAMYRPR 1538
            VDMS        K  PLEA  RPR
Sbjct: 470  VDMSEGPGLTLPKVHPLEAKCRPR 493