BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00027070
         (417 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004139935.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   129   2e-28
gb|ACO90220.1| putative O-methyltransferase [Eschscholzia califo...   129   2e-28
emb|CAQ58423.1| O-methyltransferase [Humulus lupulus]                 129   3e-28
gb|ABZ89565.1| O-methyltransferase 1 [Humulus lupulus]                129   3e-28
ref|XP_002329411.1| alkaloid o-methyltransferase related [Populu...   126   2e-27

>ref|XP_004139935.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis
           sativus] gi|449475791|ref|XP_004154552.1| PREDICTED:
           (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis
           sativus]
          Length = 357

 Score =  129 bits (324), Expect = 2e-28
 Identities = 67/123 (54%), Positives = 87/123 (70%)
 Frame = -3

Query: 415 EMAQEEAILQGQQNITKLLFAFADTMALKCVVELRIADIINSHGLPISLSEIAAGIQXXX 236
           E  + EA+LQGQ  I K +  FAD+MALKC VEL +ADIINSHG PISLS+IA+ I    
Sbjct: 5   EAKEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI-AAS 63

Query: 235 XXXSPPNINYLFRIMRLLVRKGVFSSHAPNQNEEKLYGLTNSSKWLLRDADFSMTPIIQA 56
              S P I+YL RIMRLLVR+ +F++H P+   + LYGLT+SSKWLLRD+  ++ P+  +
Sbjct: 64  NPSSAPQISYLNRIMRLLVRRNIFAAHHPSDGGDALYGLTHSSKWLLRDSPLTLAPMAFS 123

Query: 55  VTH 47
             H
Sbjct: 124 ELH 126


>gb|ACO90220.1| putative O-methyltransferase [Eschscholzia californica]
          Length = 362

 Score =  129 bits (324), Expect = 2e-28
 Identities = 72/139 (51%), Positives = 92/139 (66%), Gaps = 5/139 (3%)
 Frame = -3

Query: 403 EEAILQGQQNITKLLFAFADTMALKCVVELRIADIINSHGLPISLSEIAAGIQXXXXXXS 224
           EE  LQGQ +I KL+ AF D+MALKC VEL I DII+SHG PI+LSEI  GI        
Sbjct: 10  EEMELQGQADICKLMLAFIDSMALKCAVELGIPDIIHSHGQPITLSEIINGIPNLSPSF- 68

Query: 223 PPNINYLFRIMRLLVRKGVFSSHAPNQNE-----EKLYGLTNSSKWLLRDADFSMTPIIQ 59
             +INYLFRIMRLLVR  VFS++ P+  +     + LYGLT SSKWL++D+  S+ P++ 
Sbjct: 69  --DINYLFRIMRLLVRNRVFSAYEPDLKDGSSGTKTLYGLTPSSKWLVKDSKISLAPLVL 126

Query: 58  AVTHHCSMDSFQKLNKCVE 2
           A  H   +D +  L KCV+
Sbjct: 127 AENHPWLLDPWHYLGKCVQ 145


>emb|CAQ58423.1| O-methyltransferase [Humulus lupulus]
          Length = 352

 Score =  129 bits (323), Expect = 3e-28
 Identities = 65/120 (54%), Positives = 87/120 (72%), Gaps = 1/120 (0%)
 Frame = -3

Query: 391 LQGQQNITKLLFAFADTMALKCVVELRIADIINSHGLPISLSEIAAGIQXXXXXXSPPNI 212
           L+GQ+ I +L+F+F D+MALKC +ELRIADII+SHG PI+LS+IA+GI+        PNI
Sbjct: 4   LRGQEQIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNI 63

Query: 211 NYLFRIMRLLVRKGVFSSHAPNQNEE-KLYGLTNSSKWLLRDADFSMTPIIQAVTHHCSM 35
            YL RIMR LVRK +F+ H  + +E   LYGL++SS+WLLRD   S+ P++   TH  SM
Sbjct: 64  PYLSRIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSM 123


>gb|ABZ89565.1| O-methyltransferase 1 [Humulus lupulus]
          Length = 352

 Score =  129 bits (323), Expect = 3e-28
 Identities = 65/120 (54%), Positives = 87/120 (72%), Gaps = 1/120 (0%)
 Frame = -3

Query: 391 LQGQQNITKLLFAFADTMALKCVVELRIADIINSHGLPISLSEIAAGIQXXXXXXSPPNI 212
           L+GQ+ I +L+F+F D+MALKC +ELRIADII+SHG PI+LS+IA+GI+        PNI
Sbjct: 4   LRGQEQIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNI 63

Query: 211 NYLFRIMRLLVRKGVFSSHAPNQNEE-KLYGLTNSSKWLLRDADFSMTPIIQAVTHHCSM 35
            YL RIMR LVRK +F+ H  + +E   LYGL++SS+WLLRD   S+ P++   TH  SM
Sbjct: 64  PYLSRIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSM 123


>ref|XP_002329411.1| alkaloid o-methyltransferase related [Populus trichocarpa]
           gi|222870461|gb|EEF07592.1| alkaloid o-methyltransferase
           related [Populus trichocarpa]
          Length = 351

 Score =  126 bits (317), Expect = 2e-27
 Identities = 65/134 (48%), Positives = 93/134 (69%)
 Frame = -3

Query: 403 EEAILQGQQNITKLLFAFADTMALKCVVELRIADIINSHGLPISLSEIAAGIQXXXXXXS 224
           EEA+L+GQ  + +L+F FA++MALKC +EL IADIINS G P++L++IA+GI        
Sbjct: 6   EEALLRGQVEVWQLMFGFAESMALKCAIELGIADIINSLGGPVTLNQIASGIDSPCV--- 62

Query: 223 PPNINYLFRIMRLLVRKGVFSSHAPNQNEEKLYGLTNSSKWLLRDADFSMTPIIQAVTHH 44
             +I YL RIMR LVRK VF+ H P+   E LYGLT+SSKWLLRD++ S+ P++    + 
Sbjct: 63  --DIPYLARIMRFLVRKRVFTQHIPSDGGETLYGLTDSSKWLLRDSEVSLAPMVLMQNYP 120

Query: 43  CSMDSFQKLNKCVE 2
             +  +  L++CV+
Sbjct: 121 WQLAPWHYLSQCVK 134