BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00025892
         (425 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002315623.1| acyl:coa ligase [Populus trichocarpa] gi|222...   132   4e-29
gb|AGE10622.1| 4-coumarate CoA ligase, partial [Lonicera macrant...   128   6e-28
ref|XP_002511221.1| AMP dependent CoA ligase, putative [Ricinus ...   125   4e-27
gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]             124   1e-26
gb|ADN33954.1| 4-coumarate-CoA ligase [Cucumis melo subsp. melo]      124   1e-26

>ref|XP_002315623.1| acyl:coa ligase [Populus trichocarpa] gi|222864663|gb|EEF01794.1|
           acyl:coa ligase [Populus trichocarpa]
          Length = 552

 Score =  132 bits (331), Expect = 4e-29
 Identities = 65/125 (52%), Positives = 86/125 (68%)
 Frame = +1

Query: 46  DLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSGTR 225
           D KSGF SKT  +HSLRP +QLPP   P+SA ++AI            TTAL+D+V+G R
Sbjct: 10  DPKSGFCSKTNTYHSLRPHLQLPPITTPVSATEYAISLLLYSFPSPPQTTALLDAVTGRR 69

Query: 226 VSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANPAS 405
           +SF E +   E+L+ SL   F LKKGD AFILSPN + +PILY+SL +LGV++SP+NP S
Sbjct: 70  ISFPELIHFTETLASSLLNRFRLKKGDTAFILSPNSIHIPILYLSLFTLGVVISPSNPLS 129

Query: 406 TVSEV 420
           +  E+
Sbjct: 130 SEQEI 134


>gb|AGE10622.1| 4-coumarate CoA ligase, partial [Lonicera macranthoides]
          Length = 162

 Score =  128 bits (321), Expect = 6e-28
 Identities = 66/127 (51%), Positives = 90/127 (70%)
 Frame = +1

Query: 43  FDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSGT 222
           FD  SGFSS+T+ FHSL P + LPP    +S+A +++            +T+LIDS +G 
Sbjct: 7   FDPNSGFSSQTKTFHSLTPPLPLPPETALISSATYSLSLQLTSPWPD--STSLIDSATGQ 64

Query: 223 RVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANPA 402
           R+S+++F  Q  +LS SL++   L KGDVAFILSPN LQ+P+LY SLLSLGVI+SP+NP+
Sbjct: 65  RLSYSQFTTQTNNLSSSLQSRLGLSKGDVAFILSPNSLQIPLLYFSLLSLGVIISPSNPS 124

Query: 403 STVSEVS 423
           ST SE+S
Sbjct: 125 STESEIS 131


>ref|XP_002511221.1| AMP dependent CoA ligase, putative [Ricinus communis]
           gi|223550336|gb|EEF51823.1| AMP dependent CoA ligase,
           putative [Ricinus communis]
          Length = 549

 Score =  125 bits (314), Expect = 4e-27
 Identities = 66/128 (51%), Positives = 84/128 (65%)
 Frame = +1

Query: 40  NFDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSG 219
           N D  +GF  +T+ FHSLRP   LPP + PLS AQ+A+            TT LIDS + 
Sbjct: 7   NIDPNNGFCKETKTFHSLRPSTPLPPSDQPLSIAQYAVSLLNSTTANLTATTFLIDSNTA 66

Query: 220 TRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANP 399
             ++F++FL +  SLS SLKT+FSL   DVAFIL P  L VP+LY SLLSLG+ +SPANP
Sbjct: 67  ESLTFSQFLNRTRSLSFSLKTHFSLSPNDVAFILCPASLHVPLLYFSLLSLGLTISPANP 126

Query: 400 ASTVSEVS 423
            S+ SEV+
Sbjct: 127 LSSNSEVA 134


>gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]
          Length = 540

 Score =  124 bits (310), Expect = 1e-26
 Identities = 65/127 (51%), Positives = 89/127 (70%)
 Frame = +1

Query: 43  FDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSGT 222
           FD  SGFSS+T+ FHSL P + LPP    +S+A +++            +T LIDS +G 
Sbjct: 4   FDPNSGFSSQTKTFHSLTPPLPLPPETALISSATYSLSLQLTSPWPD--STXLIDSATGQ 61

Query: 223 RVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANPA 402
           R+S+++F  Q  +LS SL+++  L KGDVAFILS N LQ+P+LY SLLSLGVI+SP+NP+
Sbjct: 62  RLSYSQFTTQTNNLSSSLQSHLGLSKGDVAFILSLNSLQIPLLYFSLLSLGVIISPSNPS 121

Query: 403 STVSEVS 423
           ST SE+S
Sbjct: 122 STESEIS 128


>gb|ADN33954.1| 4-coumarate-CoA ligase [Cucumis melo subsp. melo]
          Length = 1055

 Score =  124 bits (310), Expect = 1e-26
 Identities = 66/129 (51%), Positives = 88/129 (68%)
 Frame = +1

Query: 37  YNFDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVS 216
           ++ DL+SGF   T+IFHSLRP + LPP + PLS  QHA             +T LIDS S
Sbjct: 499 HSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPN-STLLIDSNS 557

Query: 217 GTRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPAN 396
           G  +S+  FL+Q  +L+ +LK+  SL  G VAFIL+P  LQ+P+LY +LLSLGV++SPAN
Sbjct: 558 GLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPAN 617

Query: 397 PASTVSEVS 423
           P S+VSE+S
Sbjct: 618 PTSSVSEIS 626



 Score =  120 bits (302), Expect = 9e-26
 Identities = 64/129 (49%), Positives = 87/129 (67%)
 Frame = +1

Query: 37  YNFDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVS 216
           ++ D +SGF S+T+I+ SLRP + LPP + PL+ A HA+            T ALIDS S
Sbjct: 4   HSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPN-TAALIDSDS 62

Query: 217 GTRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPAN 396
           G  VS+  FL+Q  +L+ +LK  +S   G VAFILSP  LQ+P+LY +LLSLGV+VSPAN
Sbjct: 63  GVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSPAN 122

Query: 397 PASTVSEVS 423
           P S+ SE++
Sbjct: 123 PISSESEIA 131