BLASTX nr result
ID: Coptis25_contig00024962
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00024962
(1802 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB86897.1| syringolide-induced protein B13-1-1 [Glycine max] 893 0.0
ref|XP_002281314.2| PREDICTED: L-ascorbate oxidase-like [Vitis v... 890 0.0
ref|XP_003524331.1| PREDICTED: L-ascorbate oxidase [Glycine max] 886 0.0
emb|CBI21455.3| unnamed protein product [Vitis vinifera] 885 0.0
ref|XP_002281435.1| PREDICTED: L-ascorbate oxidase [Vitis vinife... 885 0.0
>dbj|BAB86897.1| syringolide-induced protein B13-1-1 [Glycine max]
Length = 567
Score = 893 bits (2307), Expect = 0.0
Identities = 426/568 (75%), Positives = 478/568 (84%), Gaps = 1/568 (0%)
Frame = -2
Query: 1795 IKVPVLLFVLCFIASLFASH-VEARIRHYKWEVKYEYKSPDCYKKLVIAINGRTPGPTIL 1619
+++P LL +LCF L H EARIRH+KWEVKYE++SPDC+KKLVI ING+TPGPTI
Sbjct: 1 LQLPTLL-ILCFFLILGNFHKAEARIRHHKWEVKYEFRSPDCFKKLVITINGKTPGPTIQ 59
Query: 1618 AEQGDTIVVELKNSLLTENVAIHWHGIRQIGTPWSDGTEGVSQCPILAGDTFTYKFVVDR 1439
A++GDTIVV++ NSL+TEN++IHWHGIRQIGTPW DGTEGV+QCPIL GDTF Y+FVVDR
Sbjct: 60 AQEGDTIVVQVNNSLVTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFIYQFVVDR 119
Query: 1438 PGTYLYHAHYGMQREAGLYGSIRVSVRKGVKEPFAYDEDRSIILNDWYHRSTYEQVARLS 1259
PGTYLYHAHYGMQREAGLYG IRV+ R EPFAYD DRSIILNDWYH+STYEQ A LS
Sbjct: 120 PGTYLYHAHYGMQREAGLYGMIRVAPRD--PEPFAYDLDRSIILNDWYHKSTYEQAAGLS 177
Query: 1258 SRPFQWVGEPQSLLIQGRGSFNCSILSTPNLEAGACNMSNPECSPYVITVVPGKTYRLRI 1079
S PFQWVGEPQSLLI G+G FNCS +P++ C+ SNP+CSP+V TV+PGKTYRLRI
Sbjct: 178 SIPFQWVGEPQSLLIHGKGRFNCS--KSPSVSTDVCDTSNPQCSPFVQTVIPGKTYRLRI 235
Query: 1078 SSLTSLSALNFEIEGHNMTVVEADGHNVEPFVIKNINIYSGETYSVIVKADQDPSRNYWA 899
+SLT+LSAL+FEIEGHNMTVVEADGH VEPF +KN+ IYSGETYSV+VK DQDPSRNYW
Sbjct: 236 ASLTALSALSFEIEGHNMTVVEADGHYVEPFEVKNLFIYSGETYSVLVKTDQDPSRNYWI 295
Query: 898 ASNVVSRPLHPPTGLAIFNYYPNHPKKSXXXXXXXXPLWNDTGYRLAQSNAIKARQGYIL 719
SNVVSR P GL +FNYYPNHPK+S P W+D RLAQS +IKARQGYIL
Sbjct: 296 TSNVVSRNRTTPPGLGMFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLSIKARQGYIL 355
Query: 718 PPPVTSDRVITLLNTQNEINGYRRWAVNNVSFTLPHTPYLVALKENLGHSFDQKPPPENY 539
PP TSDRVI LLNTQN I+ YR W+VNNVSFTLPHTPYL++LKEN+ +FD PPP+ Y
Sbjct: 356 KPPTTSDRVIVLLNTQNNISEYRHWSVNNVSFTLPHTPYLISLKENITGAFDPTPPPDGY 415
Query: 538 DAANYDIYQVQKNVNATSSNSIYRLKFNSTVDIILQNANTMNINNSETHPWHLHGHDFWV 359
D ANYDI+ V N NATSS+ IYRLKFN+TVDIILQNANTMN NNSETHPWHLHGHDFWV
Sbjct: 416 DFANYDIFSVASNANATSSSGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHGHDFWV 475
Query: 358 LGYGIGKFDPINDPKKYNLVNPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFYM 179
LGYG GKFD ND KKYNL NPIMKNTV VHPFGWTALRF DNPGVWAFHCHIE+HFYM
Sbjct: 476 LGYGKGKFDVNNDTKKYNLENPIMKNTVPVHPFGWTALRFRTDNPGVWAFHCHIESHFYM 535
Query: 178 GMGVVFAEGVEKVGKLPSSIMGCGATKG 95
GMGVVF EG+E+VGKLPSSIMGCG T+G
Sbjct: 536 GMGVVFEEGIERVGKLPSSIMGCGQTRG 563
>ref|XP_002281314.2| PREDICTED: L-ascorbate oxidase-like [Vitis vinifera]
Length = 730
Score = 890 bits (2299), Expect = 0.0
Identities = 418/562 (74%), Positives = 478/562 (85%), Gaps = 1/562 (0%)
Frame = -2
Query: 1777 LFVLCFIASLFASHVEARIRHYKWEVKYEYKSPDCYKKLVIAINGRTPGPTILAEQGDTI 1598
LF+L F +F + EARIR YKWEVKYEYKSPDC++K+VI ING++PGPTILAE+GDT+
Sbjct: 9 LFILLFSVLIFQA-AEARIRRYKWEVKYEYKSPDCFQKMVITINGQSPGPTILAEEGDTV 67
Query: 1597 VVELKNSLLTENVAIHWHGIRQIGTPWSDGTEGVSQCPILAGDTFTYKFVVDRPGTYLYH 1418
+VEL N LLTENVAIHWHGIRQIGTPW DGTEGV+QCPIL G+TFTY++ VDRPGTYLYH
Sbjct: 68 IVELTNGLLTENVAIHWHGIRQIGTPWFDGTEGVTQCPILPGETFTYEYKVDRPGTYLYH 127
Query: 1417 AHYGMQREAGLYGSIRVSVRKGVKEPFAYDEDRSIILNDWYHRSTYEQVARLSSRPFQWV 1238
AHYGMQREAGLYGSIRVSV +G EPF+YD DRSIIL+DWYH +TYEQV LSS PF W+
Sbjct: 128 AHYGMQREAGLYGSIRVSVARGKTEPFSYDYDRSIILSDWYHNTTYEQVLGLSSIPFDWI 187
Query: 1237 GEPQSLLIQGRGSFNCSILSTPNLEAGACNMSNPECSPYVITVVPGKTYRLRISSLTSLS 1058
GEPQSLLIQG+G +NCS++S+P + CN ++P+CSPYV+TVVPGKTYRLR+SSLTSLS
Sbjct: 188 GEPQSLLIQGKGRYNCSLVSSPYV----CNATSPQCSPYVLTVVPGKTYRLRVSSLTSLS 243
Query: 1057 ALNFEIEGHNMTVVEADGHNVEPFVIKNINIYSGETYSVIVKADQDPSRNYWAASNVVSR 878
AL+F+IEGHNMTVVEADGH VEPFVIKN+ IYSGETYSV+VKADQDPSRNYW S+VVSR
Sbjct: 244 ALSFQIEGHNMTVVEADGHFVEPFVIKNLFIYSGETYSVLVKADQDPSRNYWVTSSVVSR 303
Query: 877 P-LHPPTGLAIFNYYPNHPKKSXXXXXXXXPLWNDTGYRLAQSNAIKARQGYILPPPVTS 701
P GLAIFNYYPNHP KS PLWND R+ QS AIKA Y++PPP+TS
Sbjct: 304 NNTVTPPGLAIFNYYPNHPNKSPPTVPPAGPLWNDVEPRINQSRAIKAHHDYVIPPPLTS 363
Query: 700 DRVITLLNTQNEINGYRRWAVNNVSFTLPHTPYLVALKENLGHSFDQKPPPENYDAANYD 521
DRVI +LNTQNEINGYR+W+VNNVSF LPHTPYL+ALKEN+ FD P P+ +D NYD
Sbjct: 364 DRVILMLNTQNEINGYRKWSVNNVSFNLPHTPYLIALKENISGVFDPTPAPDYFDLENYD 423
Query: 520 IYQVQKNVNATSSNSIYRLKFNSTVDIILQNANTMNINNSETHPWHLHGHDFWVLGYGIG 341
IY N NATSSNSIYRLKFN+TVDIILQNANTM +NNSETHPWHLHGHDFWVLGYG G
Sbjct: 424 IYTKPNNSNATSSNSIYRLKFNTTVDIILQNANTMVVNNSETHPWHLHGHDFWVLGYGEG 483
Query: 340 KFDPINDPKKYNLVNPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFYMGMGVVF 161
F+ +DP+ YNL+NPIMKNT +HP+GWTALRF +DNPGVWAFHCHIE+HFYMGMGVVF
Sbjct: 484 TFNMSSDPRTYNLINPIMKNTAPIHPYGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVF 543
Query: 160 AEGVEKVGKLPSSIMGCGATKG 95
EGVE+VGKLPSSI+GCG +KG
Sbjct: 544 EEGVERVGKLPSSIIGCGKSKG 565
Score = 155 bits (391), Expect = 4e-35
Identities = 76/112 (67%), Positives = 86/112 (76%), Gaps = 1/112 (0%)
Frame = -2
Query: 958 GETYSVIVKADQDPSRNYWAASNVVSRP-LHPPTGLAIFNYYPNHPKKSXXXXXXXXPLW 782
GETYSV+VKADQDPSRNYW ++VVSR + P GLAIFNYYPNHP KS PLW
Sbjct: 618 GETYSVLVKADQDPSRNYWVTTSVVSRNNIVTPPGLAIFNYYPNHPNKSPPTVPPAGPLW 677
Query: 781 NDTGYRLAQSNAIKARQGYILPPPVTSDRVITLLNTQNEINGYRRWAVNNVS 626
ND RL QS AIKA Y++PPP+TSDRV +LNTQNEINGY+RW+VNNVS
Sbjct: 678 NDVVSRLNQSRAIKAHHDYVVPPPLTSDRVSLMLNTQNEINGYKRWSVNNVS 729
>ref|XP_003524331.1| PREDICTED: L-ascorbate oxidase [Glycine max]
Length = 577
Score = 886 bits (2289), Expect = 0.0
Identities = 423/568 (74%), Positives = 477/568 (83%), Gaps = 1/568 (0%)
Frame = -2
Query: 1795 IKVPVLLFVLCFIASLFASH-VEARIRHYKWEVKYEYKSPDCYKKLVIAINGRTPGPTIL 1619
+++P LL +LCF L H EARIRH+KWEVKYE++SPDC+KKLVI ING+TPGPTI
Sbjct: 11 LQLPTLL-ILCFFLILGNFHKAEARIRHHKWEVKYEFRSPDCFKKLVITINGKTPGPTIQ 69
Query: 1618 AEQGDTIVVELKNSLLTENVAIHWHGIRQIGTPWSDGTEGVSQCPILAGDTFTYKFVVDR 1439
A++GDTIVV++ NSL+TEN++IHWHGIRQIGTPW DGTEGV+QCPIL GDTF Y+FVVDR
Sbjct: 70 AQEGDTIVVQVNNSLVTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFIYQFVVDR 129
Query: 1438 PGTYLYHAHYGMQREAGLYGSIRVSVRKGVKEPFAYDEDRSIILNDWYHRSTYEQVARLS 1259
PGTYLYHAHYGMQREAGLYG IRV+ R EPFAYD DRSIILNDWYH+STYEQ A LS
Sbjct: 130 PGTYLYHAHYGMQREAGLYGMIRVAPRD--PEPFAYDLDRSIILNDWYHKSTYEQAAGLS 187
Query: 1258 SRPFQWVGEPQSLLIQGRGSFNCSILSTPNLEAGACNMSNPECSPYVITVVPGKTYRLRI 1079
S PFQWVGEPQSLLI G+G FNCS +P++ C+ SNP+CSP+V TV+PGKTYRLRI
Sbjct: 188 SIPFQWVGEPQSLLIHGKGRFNCS--KSPSVSTDVCDTSNPQCSPFVQTVIPGKTYRLRI 245
Query: 1078 SSLTSLSALNFEIEGHNMTVVEADGHNVEPFVIKNINIYSGETYSVIVKADQDPSRNYWA 899
+SLT+LSAL+FEIE ++MTVVEADGH VEPF +KN+ IYSGETYSV+VK DQDPSRNYW
Sbjct: 246 ASLTALSALSFEIEANDMTVVEADGHYVEPFEVKNLFIYSGETYSVLVKTDQDPSRNYWI 305
Query: 898 ASNVVSRPLHPPTGLAIFNYYPNHPKKSXXXXXXXXPLWNDTGYRLAQSNAIKARQGYIL 719
SNVVSR P GL +FNYYPNHPK+S P W+D RLAQS +IKARQGYIL
Sbjct: 306 TSNVVSRNRTTPPGLGMFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLSIKARQGYIL 365
Query: 718 PPPVTSDRVITLLNTQNEINGYRRWAVNNVSFTLPHTPYLVALKENLGHSFDQKPPPENY 539
PP TSDRVI LLNTQN I+ YR W+VNNVSFTLPHTPYL++LKEN+ +FD PPP+ Y
Sbjct: 366 KPPTTSDRVIVLLNTQNNISEYRHWSVNNVSFTLPHTPYLISLKENITGAFDPTPPPDGY 425
Query: 538 DAANYDIYQVQKNVNATSSNSIYRLKFNSTVDIILQNANTMNINNSETHPWHLHGHDFWV 359
D ANYDI+ V N NATSS+ IYRLKFN+TVDIILQNANTMN NNSETHPWHLHGHDFWV
Sbjct: 426 DFANYDIFSVASNANATSSSGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHGHDFWV 485
Query: 358 LGYGIGKFDPINDPKKYNLVNPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFYM 179
LGYG GKFD ND KKYNL NPIMKNTV VHPFGWTALRF DNPGVWAFHCHIE+HFYM
Sbjct: 486 LGYGKGKFDVNNDTKKYNLENPIMKNTVPVHPFGWTALRFRTDNPGVWAFHCHIESHFYM 545
Query: 178 GMGVVFAEGVEKVGKLPSSIMGCGATKG 95
GMGVVF EG+E+VGKLPSSIMGCG T+G
Sbjct: 546 GMGVVFEEGIERVGKLPSSIMGCGQTRG 573
>emb|CBI21455.3| unnamed protein product [Vitis vinifera]
Length = 567
Score = 885 bits (2288), Expect = 0.0
Identities = 419/562 (74%), Positives = 473/562 (84%), Gaps = 1/562 (0%)
Frame = -2
Query: 1777 LFVLCFIASLFASHVEARIRHYKWEVKYEYKSPDCYKKLVIAINGRTPGPTILAEQGDTI 1598
LF L F +F + EARIR Y+WEVKYEYKSPDC++K+VI ING +PGPTILAE+GDT+
Sbjct: 6 LFSLLFSVLMFPA-AEARIRRYRWEVKYEYKSPDCFQKMVITINGESPGPTILAEEGDTV 64
Query: 1597 VVELKNSLLTENVAIHWHGIRQIGTPWSDGTEGVSQCPILAGDTFTYKFVVDRPGTYLYH 1418
VEL NSLLTENVAIHWHGIRQIGTPW DGTEGV+QCPIL GDTFTY++ VDRPGTYLYH
Sbjct: 65 SVELTNSLLTENVAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFTYEYKVDRPGTYLYH 124
Query: 1417 AHYGMQREAGLYGSIRVSVRKGVKEPFAYDEDRSIILNDWYHRSTYEQVARLSSRPFQWV 1238
AHYGMQREAGLYGSI VSV +G EPF+YD DRSIIL DWYH +TYEQ LSS PF WV
Sbjct: 125 AHYGMQREAGLYGSICVSVARGKTEPFSYDHDRSIILTDWYHNTTYEQALGLSSIPFDWV 184
Query: 1237 GEPQSLLIQGRGSFNCSILSTPNLEAGACNMSNPECSPYVITVVPGKTYRLRISSLTSLS 1058
GEPQSLLIQG+G +NCS++S+P + CN ++P+CSPYV+TVVPGKTYRLR+SSLTSLS
Sbjct: 185 GEPQSLLIQGKGRYNCSLVSSPYV----CNSTSPQCSPYVLTVVPGKTYRLRVSSLTSLS 240
Query: 1057 ALNFEIEGHNMTVVEADGHNVEPFVIKNINIYSGETYSVIVKADQDPSRNYWAASNVVSR 878
AL+F+IEGHNMTVVEADGH VEPFVIKN+ IYSGETYSV+VKADQDPSRNYW ++VVSR
Sbjct: 241 ALSFQIEGHNMTVVEADGHFVEPFVIKNLFIYSGETYSVLVKADQDPSRNYWVTTSVVSR 300
Query: 877 P-LHPPTGLAIFNYYPNHPKKSXXXXXXXXPLWNDTGYRLAQSNAIKARQGYILPPPVTS 701
P GLAI NYYPNHPKKS PLWND R+ QS AIKA Y++PPP+TS
Sbjct: 301 NNTVTPLGLAILNYYPNHPKKSPPTVPPAGPLWNDVEPRINQSRAIKAHHDYVVPPPLTS 360
Query: 700 DRVITLLNTQNEINGYRRWAVNNVSFTLPHTPYLVALKENLGHSFDQKPPPENYDAANYD 521
DRVI +LNTQNEINGYRRW+VNNVSF LPHTPYL+ALKEN+ +FD PPP YD NYD
Sbjct: 361 DRVILMLNTQNEINGYRRWSVNNVSFNLPHTPYLIALKENITGAFDPTPPPNGYDFVNYD 420
Query: 520 IYQVQKNVNATSSNSIYRLKFNSTVDIILQNANTMNINNSETHPWHLHGHDFWVLGYGIG 341
I+ V N NATSSNSIYRL+FN+TVDIILQNANTM N+SETHPWHLHGH+FWVLGYG G
Sbjct: 421 IHNVSNNTNATSSNSIYRLQFNTTVDIILQNANTMKKNDSETHPWHLHGHNFWVLGYGKG 480
Query: 340 KFDPINDPKKYNLVNPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFYMGMGVVF 161
KFD +DP KYNL++PIMKNT VHP+GWTALRF +DNPG WAFHCHIE+HFY+GMGVVF
Sbjct: 481 KFDNFSDPIKYNLIDPIMKNTAPVHPYGWTALRFRSDNPGTWAFHCHIESHFYLGMGVVF 540
Query: 160 AEGVEKVGKLPSSIMGCGATKG 95
EGVE+VGKLPSSIMGCG KG
Sbjct: 541 EEGVERVGKLPSSIMGCGKAKG 562
>ref|XP_002281435.1| PREDICTED: L-ascorbate oxidase [Vitis vinifera]
gi|296082452|emb|CBI21457.3| unnamed protein product
[Vitis vinifera]
Length = 567
Score = 885 bits (2288), Expect = 0.0
Identities = 420/564 (74%), Positives = 473/564 (83%), Gaps = 1/564 (0%)
Frame = -2
Query: 1783 VLLFVLCFIASLFASHVEARIRHYKWEVKYEYKSPDCYKKLVIAINGRTPGPTILAEQGD 1604
++LF L F +F + EARIR Y+WEVKYEYKSPDC++K+VI ING++PGPTILAE+GD
Sbjct: 4 LVLFSLLFSVLMFPA-AEARIRRYRWEVKYEYKSPDCFQKMVITINGQSPGPTILAEEGD 62
Query: 1603 TIVVELKNSLLTENVAIHWHGIRQIGTPWSDGTEGVSQCPILAGDTFTYKFVVDRPGTYL 1424
T++VEL NSLLTENVAIHWHGIRQIGTPW DGTEGV+QCPIL GDTFTY++ VDRPGTYL
Sbjct: 63 TVIVELTNSLLTENVAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFTYEYKVDRPGTYL 122
Query: 1423 YHAHYGMQREAGLYGSIRVSVRKGVKEPFAYDEDRSIILNDWYHRSTYEQVARLSSRPFQ 1244
YHAHYGMQREAGLYGSIRVSV +G EPF+YD DRSIIL DWYH +TYEQ LSS P
Sbjct: 123 YHAHYGMQREAGLYGSIRVSVARGKTEPFSYDHDRSIILTDWYHNTTYEQALGLSSNPLD 182
Query: 1243 WVGEPQSLLIQGRGSFNCSILSTPNLEAGACNMSNPECSPYVITVVPGKTYRLRISSLTS 1064
WVGEPQSLLIQG+G +NCS++S+P + CN ++P+CSPYV+TVVPGKTYRLR+SSLTS
Sbjct: 183 WVGEPQSLLIQGKGRYNCSLVSSPYV----CNATSPQCSPYVLTVVPGKTYRLRVSSLTS 238
Query: 1063 LSALNFEIEGHNMTVVEADGHNVEPFVIKNINIYSGETYSVIVKADQDPSRNYWAASNVV 884
LSAL+F+IEGHNMTVVEADGH VEPFVIKN+ IYSGETYSV+VKADQDPSRNYW ++VV
Sbjct: 239 LSALSFQIEGHNMTVVEADGHFVEPFVIKNLFIYSGETYSVLVKADQDPSRNYWVTTSVV 298
Query: 883 SRP-LHPPTGLAIFNYYPNHPKKSXXXXXXXXPLWNDTGYRLAQSNAIKARQGYILPPPV 707
SR P GLAIFNYYPNHP KS PLWND RL QS AIKA YI+PPP
Sbjct: 299 SRNNTVTPPGLAIFNYYPNHPNKSPPTVPPVGPLWNDVEPRLNQSRAIKAHHDYIVPPPH 358
Query: 706 TSDRVITLLNTQNEINGYRRWAVNNVSFTLPHTPYLVALKENLGHSFDQKPPPENYDAAN 527
TSDRVI LNTQN+INGY RW+VNN SF LPHTPYL+ALKEN+ +FD PPP YD N
Sbjct: 359 TSDRVIVFLNTQNKINGYVRWSVNNNSFNLPHTPYLIALKENITGAFDPTPPPNGYDFVN 418
Query: 526 YDIYQVQKNVNATSSNSIYRLKFNSTVDIILQNANTMNINNSETHPWHLHGHDFWVLGYG 347
YDIY V N NATSSNSIYRL+FN+TVDIILQNANTM N+SETHPWHLHGH+FWVLGYG
Sbjct: 419 YDIYNVANNTNATSSNSIYRLQFNTTVDIILQNANTMKKNDSETHPWHLHGHNFWVLGYG 478
Query: 346 IGKFDPINDPKKYNLVNPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFYMGMGV 167
GKFD +DP KYNL++PIMKNT VHP+GWTALRF +DNPG WAFHCHIE+HFYMGMGV
Sbjct: 479 KGKFDNFSDPIKYNLIDPIMKNTAPVHPYGWTALRFRSDNPGTWAFHCHIESHFYMGMGV 538
Query: 166 VFAEGVEKVGKLPSSIMGCGATKG 95
VF EGVE+VGKLPSSIMGCG KG
Sbjct: 539 VFEEGVERVGKLPSSIMGCGKAKG 562