BLASTX nr result
ID: Coptis25_contig00024893
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00024893
(1458 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like... 785 0.0
emb|CBI31395.3| unnamed protein product [Vitis vinifera] 785 0.0
ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|2... 759 0.0
ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like... 746 0.0
ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|... 739 0.0
>ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like [Vitis vinifera]
Length = 896
Score = 785 bits (2028), Expect = 0.0
Identities = 376/485 (77%), Positives = 413/485 (85%)
Frame = +1
Query: 4 VSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSWEGCYYQYAVSVYHPSTLKIENCMAT 183
V ACIYG PSGGDPLE V LEE NGVWS GP+SWEGCYY+Y VSVYHPSTL+IE C A
Sbjct: 160 VHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTAN 219
Query: 184 DPYARGLSSDGRRTLLINLDCKNLKPERWDDLANEKPELSSFSDISIYELHIRDFSANDQ 363
DPYARGLS+D RRTLL+NLD +LKPE WD+LA+EKP L SFSDISIYELHIRDFSA+D
Sbjct: 220 DPYARGLSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDH 279
Query: 364 TVNPDLCGGYLAFTYQDSAGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDADR 543
TV+PD GGYLAFT QDSAG+LHLKKL NAG++H+HLLPTFQFAGVDDEK KWK +D +
Sbjct: 280 TVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNI 339
Query: 544 LARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQSL 723
L LPPDS EQQAQ+ IQD+DGYNWGYNPVLWGVPK LEFRKMVQ+L
Sbjct: 340 LETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQAL 399
Query: 724 NRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHFM 903
NRIG RVVLDVVYNHLHG+GP D+NSVLDKIVPGYYLRR++DG IEHS CVNNTASEHFM
Sbjct: 400 NRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFM 459
Query: 904 VERLILDDLLCWAVEYKVDGFRFDLMGHIMKRTMVKAKNTLQGLSKEIHGVDGSMIYIYG 1083
VERLILDDLLCWAV+YKVDGFRFDLMGHIMKRTMVKAKN L LSK+ G+DGS IYIYG
Sbjct: 460 VERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYG 519
Query: 1084 EGWDFGEVANNGRGINASQSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLSYQP 1263
EGWDFGEVA NGRG+NASQ NL GTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL QP
Sbjct: 520 EGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQP 579
Query: 1264 NGHEHGAKTVVDNMLAESQDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYSS 1443
N H+HG + V + MLA S+DHIQVGMA NL+DFVLTN +GEEVKG EV TY G+PVAY+
Sbjct: 580 NDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTV 639
Query: 1444 CPMET 1458
CP ET
Sbjct: 640 CPTET 644
>emb|CBI31395.3| unnamed protein product [Vitis vinifera]
Length = 956
Score = 785 bits (2028), Expect = 0.0
Identities = 376/485 (77%), Positives = 413/485 (85%)
Frame = +1
Query: 4 VSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSWEGCYYQYAVSVYHPSTLKIENCMAT 183
V ACIYG PSGGDPLE V LEE NGVWS GP+SWEGCYY+Y VSVYHPSTL+IE C A
Sbjct: 220 VHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTAN 279
Query: 184 DPYARGLSSDGRRTLLINLDCKNLKPERWDDLANEKPELSSFSDISIYELHIRDFSANDQ 363
DPYARGLS+D RRTLL+NLD +LKPE WD+LA+EKP L SFSDISIYELHIRDFSA+D
Sbjct: 280 DPYARGLSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDH 339
Query: 364 TVNPDLCGGYLAFTYQDSAGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDADR 543
TV+PD GGYLAFT QDSAG+LHLKKL NAG++H+HLLPTFQFAGVDDEK KWK +D +
Sbjct: 340 TVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNI 399
Query: 544 LARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQSL 723
L LPPDS EQQAQ+ IQD+DGYNWGYNPVLWGVPK LEFRKMVQ+L
Sbjct: 400 LETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQAL 459
Query: 724 NRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHFM 903
NRIG RVVLDVVYNHLHG+GP D+NSVLDKIVPGYYLRR++DG IEHS CVNNTASEHFM
Sbjct: 460 NRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFM 519
Query: 904 VERLILDDLLCWAVEYKVDGFRFDLMGHIMKRTMVKAKNTLQGLSKEIHGVDGSMIYIYG 1083
VERLILDDLLCWAV+YKVDGFRFDLMGHIMKRTMVKAKN L LSK+ G+DGS IYIYG
Sbjct: 520 VERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYG 579
Query: 1084 EGWDFGEVANNGRGINASQSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLSYQP 1263
EGWDFGEVA NGRG+NASQ NL GTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL QP
Sbjct: 580 EGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQP 639
Query: 1264 NGHEHGAKTVVDNMLAESQDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYSS 1443
N H+HG + V + MLA S+DHIQVGMA NL+DFVLTN +GEEVKG EV TY G+PVAY+
Sbjct: 640 NDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTV 699
Query: 1444 CPMET 1458
CP ET
Sbjct: 700 CPTET 704
>ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|222864374|gb|EEF01505.1|
predicted protein [Populus trichocarpa]
Length = 893
Score = 759 bits (1959), Expect = 0.0
Identities = 360/486 (74%), Positives = 408/486 (83%)
Frame = +1
Query: 1 AVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSWEGCYYQYAVSVYHPSTLKIENCMA 180
AV AC+Y + DP+E VQL+EVNGVWS +G + WEGCYY Y VSVYHPSTL +E C A
Sbjct: 158 AVCACVYKNANSRDPVEVVQLKEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYA 217
Query: 181 TDPYARGLSSDGRRTLLINLDCKNLKPERWDDLANEKPELSSFSDISIYELHIRDFSAND 360
DPYARGLS D +RTL +NLD LKPE W+ LA+EKP + SFSDISIYELH+RDFSAND
Sbjct: 218 NDPYARGLSPDSQRTLFVNLDSDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSAND 277
Query: 361 QTVNPDLCGGYLAFTYQDSAGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDAD 540
TV+PD GGYLAFT +DSAG+LHLKKLSNAG+THVHLLPTFQFAGVDD K+ WK +D+
Sbjct: 278 HTVHPDFRGGYLAFTLEDSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDVKENWKCVDST 337
Query: 541 RLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQS 720
L +LPPDS EQQAQ+T IQDDDGYNWGYNPVLWGVPK +EFRKMVQ+
Sbjct: 338 VLEKLPPDSTEQQAQITLIQDDDGYNWGYNPVLWGVPKGSYASNPSGSCRTIEFRKMVQA 397
Query: 721 LNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHF 900
LN IGLRVVLDVVYNHLHGNGP D+NSVLDKIVPGYYLRR+TDG IEHSTCVNNTASEH+
Sbjct: 398 LNHIGLRVVLDVVYNHLHGNGPFDENSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHY 457
Query: 901 MVERLILDDLLCWAVEYKVDGFRFDLMGHIMKRTMVKAKNTLQGLSKEIHGVDGSMIYIY 1080
MVER+I+DD+L WA YKVDGFRFDLMGHIMK TMVKAK+ + L+KE G+DGS +YIY
Sbjct: 458 MVERMIIDDMLNWAGNYKVDGFRFDLMGHIMKSTMVKAKDAVNRLTKERDGIDGSSVYIY 517
Query: 1081 GEGWDFGEVANNGRGINASQSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLSYQ 1260
GEGWDFGEVANNGRGINASQ N+ GTGIGSFNDRIRDA+LGGSPFGHPLQQGF+TGL Q
Sbjct: 518 GEGWDFGEVANNGRGINASQFNVGGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLMLQ 577
Query: 1261 PNGHEHGAKTVVDNMLAESQDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYS 1440
PNGH+HG K V + ML+ ++DHIQVGMAGNLRD+VLTN G+EVKG+EVLTYGG+PVAY+
Sbjct: 578 PNGHDHGGKDVEELMLSAAKDHIQVGMAGNLRDYVLTNSDGKEVKGMEVLTYGGAPVAYA 637
Query: 1441 SCPMET 1458
P ET
Sbjct: 638 LHPAET 643
>ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like [Glycine max]
Length = 951
Score = 746 bits (1927), Expect = 0.0
Identities = 356/486 (73%), Positives = 405/486 (83%)
Frame = +1
Query: 1 AVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSWEGCYYQYAVSVYHPSTLKIENCMA 180
AV A IY PSG DP+E V LEE NGVW GP+SWEGCYY Y V VYHPST++IE C
Sbjct: 214 AVHAYIYKDPSGDDPIEIVCLEEENGVWRTKGPKSWEGCYYVYEVCVYHPSTMRIEKCYT 273
Query: 181 TDPYARGLSSDGRRTLLINLDCKNLKPERWDDLANEKPELSSFSDISIYELHIRDFSAND 360
+DPYARGLSSDGRR+ L+NLD LKP+ WD+LAN+KP + SFSDISIYE+HIRDFSA+D
Sbjct: 274 SDPYARGLSSDGRRSFLLNLDSVKLKPDGWDNLANKKPTIHSFSDISIYEMHIRDFSASD 333
Query: 361 QTVNPDLCGGYLAFTYQDSAGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDAD 540
+V P+ GGYLAFT QDSAG+LHLKKLS+AG+THVHLLPTFQFAGVDD+K+ W+ +D
Sbjct: 334 LSVQPEFRGGYLAFTLQDSAGVLHLKKLSSAGITHVHLLPTFQFAGVDDQKEDWRFVDTS 393
Query: 541 RLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQS 720
L LPPDSD+QQA +TAIQ+ DGYNWGYNPVLWGVPK +EFRKMV +
Sbjct: 394 ILESLPPDSDQQQALITAIQNFDGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVMA 453
Query: 721 LNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHF 900
LN IGLRVVLDVVYNHL G+GP D++SVLDKIVPGYYLRR++DG IEHSTC+NNTASEHF
Sbjct: 454 LNHIGLRVVLDVVYNHLQGSGPFDEHSVLDKIVPGYYLRRNSDGLIEHSTCINNTASEHF 513
Query: 901 MVERLILDDLLCWAVEYKVDGFRFDLMGHIMKRTMVKAKNTLQGLSKEIHGVDGSMIYIY 1080
MVERLILDDL+ WAV YK+DGFRFDLMGHIMK TMVKAK LQ L+KE G+DGS IYIY
Sbjct: 514 MVERLILDDLVHWAVNYKIDGFRFDLMGHIMKSTMVKAKTALQCLTKEKDGLDGSSIYIY 573
Query: 1081 GEGWDFGEVANNGRGINASQSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLSYQ 1260
GEGWDFGEVA NGRG+NASQ NL GT IGSFNDRIRDAILGGSPFGHPLQQGF+TGL Q
Sbjct: 574 GEGWDFGEVAKNGRGVNASQFNLPGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQ 633
Query: 1261 PNGHEHGAKTVVDNMLAESQDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYS 1440
PNGH+HG + +MLA S DHIQ+GMA NL+DFVLTN +GEEVKG E+LTYGG+PVAY+
Sbjct: 634 PNGHDHGTEANAKSMLAASMDHIQIGMAANLKDFVLTNSEGEEVKGSEILTYGGTPVAYA 693
Query: 1441 SCPMET 1458
SCP+ET
Sbjct: 694 SCPIET 699
>ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|223527468|gb|EEF29599.1|
pullulanase, putative [Ricinus communis]
Length = 964
Score = 739 bits (1908), Expect = 0.0
Identities = 357/486 (73%), Positives = 400/486 (82%)
Frame = +1
Query: 1 AVSACIYGAPSGGDPLETVQLEEVNGVWSFDGPRSWEGCYYQYAVSVYHPSTLKIENCMA 180
AV IY PLE QL+EVNGVWS GP+ WEGCYY Y VSVYHPSTL+IE C A
Sbjct: 229 AVLVYIYKDSFSKVPLEIHQLKEVNGVWSIKGPKDWEGCYYVYEVSVYHPSTLRIEKCYA 288
Query: 181 TDPYARGLSSDGRRTLLINLDCKNLKPERWDDLANEKPELSSFSDISIYELHIRDFSAND 360
DPYARGLSSDG+RTLL+NL ++LKPE WD+LA EKP L SF DIS+YELHIRDFSAND
Sbjct: 289 NDPYARGLSSDGKRTLLVNLTSESLKPEGWDNLAKEKPSLLSFCDISLYELHIRDFSAND 348
Query: 361 QTVNPDLCGGYLAFTYQDSAGLLHLKKLSNAGLTHVHLLPTFQFAGVDDEKQKWKNLDAD 540
QTV+PD GGYLAFT+++SAG+LHLKKLSNAG+THVHLLPTFQFAGVDD K+ WK +D
Sbjct: 349 QTVHPDFRGGYLAFTFENSAGVLHLKKLSNAGITHVHLLPTFQFAGVDDVKENWKCVDNK 408
Query: 541 RLARLPPDSDEQQAQVTAIQDDDGYNWGYNPVLWGVPKXXXXXXXXXXXXXLEFRKMVQS 720
L L PDS EQQAQ+TA+QD+DGYNWGYNPVLWGVPK ++FRKMVQ+
Sbjct: 409 MLETLLPDSIEQQAQITAVQDNDGYNWGYNPVLWGVPKGSYASNPNGPSRTIQFRKMVQA 468
Query: 721 LNRIGLRVVLDVVYNHLHGNGPHDDNSVLDKIVPGYYLRRSTDGSIEHSTCVNNTASEHF 900
LNRIGLRVVLDVVYNHLHG+GP D+NSVLDKIVPGYYLRR+ DG IE+STCVNNTASEH+
Sbjct: 469 LNRIGLRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNIDGIIENSTCVNNTASEHY 528
Query: 901 MVERLILDDLLCWAVEYKVDGFRFDLMGHIMKRTMVKAKNTLQGLSKEIHGVDGSMIYIY 1080
MVERLI+DDLL WAV YKVDGFRFDLMGH+MK TMVKAK L LS E GVDGS +YIY
Sbjct: 529 MVERLIIDDLLNWAVNYKVDGFRFDLMGHLMKSTMVKAKIALHSLSVERDGVDGSSLYIY 588
Query: 1081 GEGWDFGEVANNGRGINASQSNLHGTGIGSFNDRIRDAILGGSPFGHPLQQGFLTGLSYQ 1260
GEGWDFGEVA NGRG+NASQ NL+GTGIGSFNDRIRDA+LGGSPFGHPL QGF+TGL Q
Sbjct: 589 GEGWDFGEVAKNGRGVNASQFNLYGTGIGSFNDRIRDAMLGGSPFGHPLHQGFVTGLMLQ 648
Query: 1261 PNGHEHGAKTVVDNMLAESQDHIQVGMAGNLRDFVLTNFQGEEVKGLEVLTYGGSPVAYS 1440
PNGH+HG K V + ML ++DHIQVGMA NLRDFVL N +G+EVKG E+ TYGG PVAY+
Sbjct: 649 PNGHDHGGKDVEELMLTTAKDHIQVGMAANLRDFVLINSEGKEVKGSEITTYGGEPVAYA 708
Query: 1441 SCPMET 1458
P ET
Sbjct: 709 LSPTET 714