BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00022872
         (1916 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid ...   682   0.0  
dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]           667   0.0  
ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinu...   667   0.0  
gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]       662   0.0  
ref|XP_002879389.1| ent-kaurenoic acid hydroxylase [Arabidopsis ...   661   0.0  

>ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222862021|gb|EEE99563.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 493

 Score =  682 bits (1761), Expect(2) = 0.0
 Identities = 321/455 (70%), Positives = 389/455 (85%), Gaps = 1/455 (0%)
 Frame = -2

Query: 1651 KVVSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGMYKAFMFGNPTIMVT 1472
            K  SLPPGD+GWPFIGNMWSFLRAFKS+DP+SF+  F+ ++G  G+YKAFMFGNP++ VT
Sbjct: 39   KQYSLPPGDLGWPFIGNMWSFLRAFKSSDPDSFMRTFINKYGDNGIYKAFMFGNPSVFVT 98

Query: 1471 IPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVY 1292
             PE CR+VL +D+ FKPGWP ST++LIGRKSF+ +SYE+HK LRR+T+APVNGHEALSVY
Sbjct: 99   TPEACRRVLSNDDAFKPGWPISTLKLIGRKSFIDISYEEHKRLRRLTSAPVNGHEALSVY 158

Query: 1291 LEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGESVMDALEEVYTTLNY 1112
            + +IEENV++ LE+W+ MG+IEFLTQ+RKLTF+I+M+IF+S   E VM+ALE+ YTTLNY
Sbjct: 159  IPYIEENVIAMLEKWTTMGKIEFLTQVRKLTFKIIMYIFLSSESEVVMEALEKDYTTLNY 218

Query: 1111 GVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKK-DMLDNLLDVKD 935
            GVRAMAINLPGFAYY+ALK+RKRLV IF S+V+ERR +R      AKK DM+D+LL V+D
Sbjct: 219  GVRAMAINLPGFAYYKALKARKRLVAIFQSIVDERRNLRKNSARNAKKKDMMDSLLGVED 278

Query: 934  ENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRSS 755
            EN RKL DEEIID ++MYLNAGHESSGHIT WAT+ LQEHP+  QKAK EQEQIVK R  
Sbjct: 279  ENGRKLTDEEIIDVILMYLNAGHESSGHITTWATIFLQEHPEFLQKAKEEQEQIVKRRPP 338

Query: 754  MQKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRN 575
             Q GL LKE+++MDYL+KVIDETLRLI+FSL VFREAK D S+NGYIIP GWKV VW R 
Sbjct: 339  AQNGLSLKEVREMDYLSKVIDETLRLITFSLTVFREAKTDFSINGYIIPKGWKVLVWFRT 398

Query: 574  VHRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNY 395
            VH DPE+Y NP +F+PSRWD +T KAGT+LPFGAGSRLCPGN+LAKLEI+IF+HYFLL+Y
Sbjct: 399  VHLDPEIYQNPKEFNPSRWDNYTPKAGTFLPFGAGSRLCPGNDLAKLEISIFLHYFLLDY 458

Query: 394  RVERLNPKCPVMYLPHTRHTDNCLARIRKISPPSM 290
            R+ER NP+C  M+LPHTR TDNC+ARI+K+S  S+
Sbjct: 459  RLERENPECRWMFLPHTRPTDNCVARIKKVSSTSV 493



 Score = 26.2 bits (56), Expect(2) = 0.0
 Identities = 10/17 (58%), Positives = 13/17 (76%)
 Frame = -3

Query: 1692 KKANEWNYESKLGEKWF 1642
            KK N W YE++LGEK +
Sbjct: 25   KKVNWWLYEAQLGEKQY 41


>dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]
          Length = 493

 Score =  667 bits (1722), Expect = 0.0
 Identities = 310/446 (69%), Positives = 374/446 (83%)
 Frame = -2

Query: 1639 LPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGMYKAFMFGNPTIMVTIPET 1460
            LPPGDMGWPFIGNMWSFLRAFKSNDP++FI+ F++RFG  G+YK+FMFG+P+I+VT+PE 
Sbjct: 47   LPPGDMGWPFIGNMWSFLRAFKSNDPDAFISNFLRRFGSRGLYKSFMFGSPSIIVTVPEA 106

Query: 1459 CRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFI 1280
            CR+VL DDE F+PGWP +T ELIG+KSF+G+SYEDHK LR++TAAPVNGHEALS Y+++I
Sbjct: 107  CRKVLFDDEAFQPGWPTATRELIGKKSFIGISYEDHKRLRKLTAAPVNGHEALSTYMQYI 166

Query: 1279 EENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGESVMDALEEVYTTLNYGVRA 1100
            E NVV  LE+WS MG IEFLT LRKLTF+I+MHIF+S   E VMDALE+ YT LNYGVRA
Sbjct: 167  ETNVVLALEKWSKMGRIEFLTYLRKLTFQIIMHIFLSTESEHVMDALEKEYTVLNYGVRA 226

Query: 1099 MAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKKDMLDNLLDVKDENDRK 920
            MAIN+PGFAYY ALK+RK LV I  +VV ERR  R  +   +KKDMLD LLD  DE  RK
Sbjct: 227  MAINIPGFAYYNALKARKNLVAILQTVVYERRKKREANEGMSKKDMLDALLDTTDEKGRK 286

Query: 919  LNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRSSMQKGL 740
            L+DEEIIDTL+MYLNAGHESSGHITMW+T+LLQ HP+ F  AK EQE+IVKN    Q+GL
Sbjct: 287  LDDEEIIDTLVMYLNAGHESSGHITMWSTILLQAHPEFFHIAKEEQERIVKNMPPTQEGL 346

Query: 739  RLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDP 560
             LKE +QM+YL+KVIDETLRL++FSL+ FREAKKDV + GY IP GWKV +W R+VH +P
Sbjct: 347  TLKEYRQMEYLSKVIDETLRLVTFSLMTFREAKKDVDIKGYFIPKGWKVLLWFRSVHHNP 406

Query: 559  EVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERL 380
            E+YP P +F+PSRWD    K GT+LPFGAGSRLCPGN+LAKLEIAIF+H+FL+NY  ER 
Sbjct: 407  ELYPQPKEFNPSRWDDLVPKPGTFLPFGAGSRLCPGNDLAKLEIAIFLHHFLVNYEFERE 466

Query: 379  NPKCPVMYLPHTRHTDNCLARIRKIS 302
            NP+CP+MYLPH+R  DNC+ R+R++S
Sbjct: 467  NPECPIMYLPHSRPKDNCVGRVRRVS 492


>ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinus communis]
            gi|223534199|gb|EEF35915.1| Ent-kaurenoic acid oxidase,
            putative [Ricinus communis]
          Length = 492

 Score =  667 bits (1721), Expect = 0.0
 Identities = 313/448 (69%), Positives = 378/448 (84%), Gaps = 2/448 (0%)
 Frame = -2

Query: 1642 SLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGMYKAFMFGNPTIMVTIPE 1463
            SLPPGD+GWPFIGNMWSFLRAFKSNDP+SF+  F  R+G  G+YKAFMFG P+++VT  E
Sbjct: 42   SLPPGDLGWPFIGNMWSFLRAFKSNDPDSFMRNFTARYGSGGIYKAFMFGKPSVLVTTSE 101

Query: 1462 TCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEF 1283
             C++VL DD+ FKPGWP ST ELIG+KSF+G+SYE+HK LRR+TA+PVNGHEALSVY+ +
Sbjct: 102  ACKRVLTDDDAFKPGWPSSTTELIGKKSFIGISYEEHKRLRRLTASPVNGHEALSVYMHY 161

Query: 1282 IEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGESVMDALEEVYTTLNYGVR 1103
            IE+ V S LE+WS MGEI+FLTQLRKLTFRI+M+IF+S    SVM+ALE  YTTLNYGVR
Sbjct: 162  IEDKVKSALEKWSTMGEIQFLTQLRKLTFRIIMYIFLSSESHSVMEALEREYTTLNYGVR 221

Query: 1102 AMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPA--KKDMLDNLLDVKDEN 929
            AMAINLPGFAYY+ALK+RK LV +   VV+ RR  +    P +  KKDM+D LLDV+DE 
Sbjct: 222  AMAINLPGFAYYKALKARKNLVAVLQFVVDARRNQKKGAEPNSSKKKDMMDALLDVEDEK 281

Query: 928  DRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRSSMQ 749
             RKL+DEEI+D L+MYLNAGHESSGHITMWATV LQEHP+  QKA+ EQE+I+K R   Q
Sbjct: 282  GRKLSDEEIVDVLLMYLNAGHESSGHITMWATVFLQEHPEFLQKARQEQEEIIKRRPPTQ 341

Query: 748  KGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVH 569
            KGL LKE++ M+YL+KVIDETLRLI+FSLVVFREAK +V+++GY+IP GWK+ VW R+VH
Sbjct: 342  KGLTLKEVRDMEYLSKVIDETLRLITFSLVVFREAKTNVNISGYVIPKGWKILVWFRSVH 401

Query: 568  RDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRV 389
             DPE+YPNP +F+PSRWD  T KAGT+LPFGAGSR+CPGN+LAKLEIAIF+H+FLLNY +
Sbjct: 402  LDPEIYPNPREFNPSRWDNHTAKAGTFLPFGAGSRMCPGNDLAKLEIAIFLHHFLLNYEL 461

Query: 388  ERLNPKCPVMYLPHTRHTDNCLARIRKI 305
            ERLNP   +MYLPH+R  DNCLARI+KI
Sbjct: 462  ERLNPGSSMMYLPHSRPKDNCLARIKKI 489


>gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]
          Length = 492

 Score =  662 bits (1709), Expect(2) = 0.0
 Identities = 310/454 (68%), Positives = 378/454 (83%)
 Frame = -2

Query: 1651 KVVSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGMYKAFMFGNPTIMVT 1472
            K  SLPPGD+GWPFIGNMWSFL AFKS DP+SF++ FV RFGR G+YK FMFGNP+++VT
Sbjct: 39   KQYSLPPGDLGWPFIGNMWSFLSAFKSKDPDSFVSSFVSRFGRTGIYKVFMFGNPSVIVT 98

Query: 1471 IPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVY 1292
             PE CR+VL DD+ FKPGWP+STV LIG+KSF+G+S+E+HK LRR+TAAPVNG+EALS+Y
Sbjct: 99   SPEACRRVLSDDDCFKPGWPKSTVALIGKKSFIGISFEEHKRLRRLTAAPVNGYEALSMY 158

Query: 1291 LEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGESVMDALEEVYTTLNY 1112
             ++IEE V S+L++W+ MGEIEFLT+LRKLTFRI+M+IF+S   E VM ALE  YT LNY
Sbjct: 159  TKYIEEIVTSSLDKWTTMGEIEFLTELRKLTFRIIMYIFLSSESEPVMTALEREYTALNY 218

Query: 1111 GVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKKDMLDNLLDVKDE 932
            GVRAMAIN+PGFAY++ALK+RK LV  F S+V++RR  +    P  KKDM+D L+DVKDE
Sbjct: 219  GVRAMAINIPGFAYHKALKARKNLVATFQSIVDDRRNQKKVSVPTKKKDMMDALIDVKDE 278

Query: 931  NDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRSSM 752
            N RKL DEEIID L+MYLNAGHESSGH  MWAT+ L +HP+  QKAK EQE+IV+ R   
Sbjct: 279  NGRKLTDEEIIDILVMYLNAGHESSGHTAMWATIFLNQHPEYLQKAKKEQEEIVRKRLPE 338

Query: 751  QKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNV 572
            QKGL LKEI++M+YL+KVIDETLR+I+FSL VFREAK+DV++ GY IP GW+V VW R+V
Sbjct: 339  QKGLTLKEIREMEYLSKVIDETLRVITFSLTVFREAKQDVNIAGYTIPKGWRVLVWFRSV 398

Query: 571  HRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYR 392
            H DPE+Y NP +F PSRWD FT KAG +LPFGAG+RLCPGN+LAKLEI+IF+H+FLLNY+
Sbjct: 399  HLDPEIYENPKEFKPSRWDNFTPKAGAFLPFGAGTRLCPGNDLAKLEISIFLHHFLLNYQ 458

Query: 391  VERLNPKCPVMYLPHTRHTDNCLARIRKISPPSM 290
            +ERLNP  P MYLPHTR  DNCLARI+K    S+
Sbjct: 459  LERLNPGSPSMYLPHTRPVDNCLARIKKCPSSSL 492



 Score = 25.8 bits (55), Expect(2) = 0.0
 Identities = 10/17 (58%), Positives = 13/17 (76%)
 Frame = -3

Query: 1692 KKANEWNYESKLGEKWF 1642
            K AN W YE++LGEK +
Sbjct: 25   KNANWWLYETQLGEKQY 41


>ref|XP_002879389.1| ent-kaurenoic acid hydroxylase [Arabidopsis lyrata subsp. lyrata]
            gi|297325228|gb|EFH55648.1| ent-kaurenoic acid
            hydroxylase [Arabidopsis lyrata subsp. lyrata]
          Length = 489

 Score =  661 bits (1706), Expect(2) = 0.0
 Identities = 310/447 (69%), Positives = 377/447 (84%)
 Frame = -2

Query: 1639 LPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGMYKAFMFGNPTIMVTIPET 1460
            LPPGD+GWP IGNMWSFLRAFK++DPESFI  ++ R+GR G+YKA MFG P ++VT PET
Sbjct: 44   LPPGDLGWPIIGNMWSFLRAFKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPET 103

Query: 1459 CRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRITAAPVNGHEALSVYLEFI 1280
            CR+VL DD+ F  GWP+ST++LIGRKSFVG+S+E+HK LRR+T+APVNG EALSVY++FI
Sbjct: 104  CRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFI 163

Query: 1279 EENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGESVMDALEEVYTTLNYGVRA 1100
            EE V++ LE+WS MGEIEFL+ LRKLTF+++M+IF+S   E VMDALE  YT LNYGVRA
Sbjct: 164  EETVITDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSSESEHVMDALEREYTNLNYGVRA 223

Query: 1099 MAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPAKKDMLDNLLDVKDENDRK 920
            M INLPGFAY+RALK+RK+LV  F S+V  RR  R ++    +KDMLDNL+DVKDEN R 
Sbjct: 224  MGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV 283

Query: 919  LNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKAKAEQEQIVKNRSSMQKGL 740
            L+DEEIID L+MYLNAGHESSGH+TMWAT+L+QEHP++ QKAK EQE+IVKNR+  QK L
Sbjct: 284  LDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPEILQKAKEEQERIVKNRALGQK-L 342

Query: 739  RLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYIIPMGWKVEVWLRNVHRDP 560
             LKE ++M+YL++VIDETLR+I+FSL  FREAK DV ++GYIIP GWKV  W RNVH DP
Sbjct: 343  TLKETREMEYLSQVIDETLRVITFSLTAFREAKSDVQIDGYIIPKGWKVLTWFRNVHLDP 402

Query: 559  EVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKLEIAIFMHYFLLNYRVERL 380
            E+YP+P KFDPSRW+G+T KAGT+LPFG GS LCPGN+LAKLEI+IF+H+FLL YRVER 
Sbjct: 403  EIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLRYRVERS 462

Query: 379  NPKCPVMYLPHTRHTDNCLARIRKISP 299
            NP CPVM+LPH R  DNCLARI K +P
Sbjct: 463  NPGCPVMFLPHNRPKDNCLARITKTTP 489



 Score = 26.9 bits (58), Expect(2) = 0.0
 Identities = 11/15 (73%), Positives = 11/15 (73%)
 Frame = -3

Query: 1692 KKANEWNYESKLGEK 1648
            K  N W YESKLGEK
Sbjct: 26   KSVNVWIYESKLGEK 40