BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00022746
         (463 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]      116   2e-24
ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|2...   112   2e-23
gb|AFI23414.1| pectin methylesterase [Coffea arabica]                 112   3e-23
ref|XP_003547912.1| PREDICTED: pectinesterase 3-like [Glycine max]    107   1e-21
ref|XP_002532945.1| Pectinesterase-3 precursor, putative [Ricinu...   107   1e-21

>ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]
          Length = 578

 Score =  116 bits (291), Expect = 2e-24
 Identities = 61/133 (45%), Positives = 89/133 (66%), Gaps = 1/133 (0%)
 Frame = -2

Query: 396 MDTVKSFKGYGKVDEVEERTFRQKTRRRXXXXXXXXXXXXXXXXXXXXXXXVHNNNNKSD 217
           MD++KSFKGYGKVDE+EER F++KTR+R                       +H + ++S+
Sbjct: 1   MDSIKSFKGYGKVDELEERAFKRKTRKRLIILIISSVVLVAVIIGAVAGTLIHKSKSESN 60

Query: 216 SKNPT-TSTAQTIKGVCSVTQYPESCISSLSSVQDSNKITDPKQLFKLSMQIAINEISRI 40
           S   +  S A +IK VCSVTQYP+SC+SS+SS+  SN  TDP++LF+L++++AI E+S++
Sbjct: 61  SVPASPVSPATSIKAVCSVTQYPDSCVSSISSLDTSN-TTDPEELFRLTLRVAIAELSKL 119

Query: 39  SSLPDQFISNLND 1
           SSLP Q  +  ND
Sbjct: 120 SSLPRQLSAKSND 132


>ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|222841688|gb|EEE79235.1|
           predicted protein [Populus trichocarpa]
          Length = 588

 Score =  112 bits (281), Expect = 2e-23
 Identities = 58/135 (42%), Positives = 83/135 (61%), Gaps = 3/135 (2%)
 Frame = -2

Query: 396 MDTVKSFKGYGKVDEVEERTFRQKTRRRXXXXXXXXXXXXXXXXXXXXXXXVHNNNNKSD 217
           MD++ SFKGYGKVDE E++ FR+K+R+R                       VH  NNKS 
Sbjct: 1   MDSINSFKGYGKVDEAEQQAFRKKSRKRLIIIVVSSIVLLAVIIGAVIGAVVHKRNNKSS 60

Query: 216 SK---NPTTSTAQTIKGVCSVTQYPESCISSLSSVQDSNKITDPKQLFKLSMQIAINEIS 46
           S     P  + A ++K VCSVTQYP SC SS+S+++  N  TDP+ LFKLS+++A+NE+S
Sbjct: 61  SNPVPPPELTPATSLKAVCSVTQYPASCFSSISALETGN-TTDPEVLFKLSLRVAMNELS 119

Query: 45  RISSLPDQFISNLND 1
           ++   PD+ I ++ D
Sbjct: 120 KLKDYPDKLIQSIKD 134


>gb|AFI23414.1| pectin methylesterase [Coffea arabica]
          Length = 582

 Score =  112 bits (280), Expect = 3e-23
 Identities = 55/133 (41%), Positives = 84/133 (63%), Gaps = 1/133 (0%)
 Frame = -2

Query: 396 MDTVKSFKGYGKVDEVEERTFRQKTRRRXXXXXXXXXXXXXXXXXXXXXXXVHNN-NNKS 220
           MDT+KSFKGYGKVD VEE+ FR+KTRRR                        H   N+K 
Sbjct: 2   MDTIKSFKGYGKVDPVEEQAFRRKTRRRIIVLLVSLLLLVALIVGVVVATVAHKKKNSKG 61

Query: 219 DSKNPTTSTAQTIKGVCSVTQYPESCISSLSSVQDSNKITDPKQLFKLSMQIAINEISRI 40
           ++ +  TS +Q+++ +CSVT +P+SC +S+SS++ SN  TDP++LF+LS+Q+    + ++
Sbjct: 62  NANDAPTSPSQSLRAICSVTVHPDSCYTSISSLEASNSTTDPEKLFQLSLQVVHASLQKL 121

Query: 39  SSLPDQFISNLND 1
           S+LP  +IS+  D
Sbjct: 122 STLPQHWISDARD 134


>ref|XP_003547912.1| PREDICTED: pectinesterase 3-like [Glycine max]
          Length = 586

 Score =  107 bits (267), Expect = 1e-21
 Identities = 59/134 (44%), Positives = 83/134 (61%), Gaps = 5/134 (3%)
 Frame = -2

Query: 396 MDTVKSFKGYGKVDEVEERTFRQKTRRRXXXXXXXXXXXXXXXXXXXXXXXVHNNNN--- 226
           MDT+KSFKGYGKVDE+E++ +++KTR+R                       +H  N    
Sbjct: 1   MDTIKSFKGYGKVDELEQQAYQKKTRKRLIIITVSSIVLIAVIIAAIAGVVIHKRNTSSS 60

Query: 225 -KSDSKNPTTST-AQTIKGVCSVTQYPESCISSLSSVQDSNKITDPKQLFKLSMQIAINE 52
             SDS   T  T A ++K VC VTQYP SC S++SS+ DSN  TDP+ LFKLS+++AI+E
Sbjct: 61  PSSDSPPQTELTPAASLKAVCDVTQYPNSCFSAISSLPDSN-TTDPELLFKLSLRVAIDE 119

Query: 51  ISRISSLPDQFISN 10
           +S++SS P +  +N
Sbjct: 120 LSKLSSFPSKLRAN 133


>ref|XP_002532945.1| Pectinesterase-3 precursor, putative [Ricinus communis]
           gi|223527274|gb|EEF29429.1| Pectinesterase-3 precursor,
           putative [Ricinus communis]
          Length = 589

 Score =  107 bits (266), Expect = 1e-21
 Identities = 58/134 (43%), Positives = 82/134 (61%), Gaps = 3/134 (2%)
 Frame = -2

Query: 396 MDTVKSFKGYGKVDEVEERTFRQKTRRRXXXXXXXXXXXXXXXXXXXXXXXVHNNNNKS- 220
           MD++ SFKGYGKVDE+E++ +R+KTR+R                       +H  NN S 
Sbjct: 1   MDSINSFKGYGKVDELEQQAYRRKTRKRLIILIVSFIVLVAIIIGAVVGTVIHKRNNDSS 60

Query: 219 -DSKNPTTST-AQTIKGVCSVTQYPESCISSLSSVQDSNKITDPKQLFKLSMQIAINEIS 46
            +S  PT  T A ++K VCSVTQ+P SC SS+SS++ +N  +DP+ LFKLS+ + INE+S
Sbjct: 61  PNSAVPTELTPAASLKAVCSVTQFPSSCFSSISSLETAN-TSDPEVLFKLSLHVVINELS 119

Query: 45  RISSLPDQFISNLN 4
           +I   P + I N N
Sbjct: 120 KIKDYPSKLIQNSN 133