BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00022642
         (1007 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ...   328   1e-98
dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       323   2e-95
emb|CBI33076.3| unnamed protein product [Vitis vinifera]              315   8e-94
ref|XP_003635296.1| PREDICTED: secologanin synthase-like [Vitis ...   315   8e-94
ref|XP_002516130.1| cytochrome P450, putative [Ricinus communis]...   315   9e-92

>ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
            vinifera]
          Length = 520

 Score =  328 bits (842), Expect(2) = 1e-98
 Identities = 161/302 (53%), Positives = 217/302 (71%), Gaps = 1/302 (0%)
 Frame = -3

Query: 1005 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 826
            I+GTPYK + GD+KE      EAWSKP+NL HHIV RV PF    ++ YGK+S FW GT 
Sbjct: 44   IRGTPYKLVMGDMKEYIRLITEAWSKPMNLNHHIVSRVDPFTQNNMQQYGKVSLFWAGTT 103

Query: 825  PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 646
            P LI++DP ++KEVLS+K G  QKP  +P I  L+ GL++L+GE W K R++I+PAFH E
Sbjct: 104  PRLIVMDPGMIKEVLSNKQGHFQKPYISPLILTLARGLTALEGEVWAKXRRIINPAFHLE 163

Query: 645  KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 466
            KLK M PAF TSC  LIE+WK+    Q  CE+D W ++Q+ T DVISR A GS+FEEG++
Sbjct: 164  KLKVMIPAFTTSCSMLIERWKELASLQETCEVDIWPELQNLTRDVISRAALGSSFEEGRQ 223

Query: 465  IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 286
            IFELQKE   L ++A+Q++YIPGFRFIPTKKN+RR  L K      RDLI+RK  A+ +G
Sbjct: 224  IFELQKEHITLTLEAMQTLYIPGFRFIPTKKNQRRKYLQKRTTSMFRDLIQRKKDAIRTG 283

Query: 285  KSSVDDLLGMLLQYSRESS-QADMNNLKTTGMSIDQVIEECKLFYFAGQETSSNLLTWTM 109
            ++  D+LLG+LL  S +++   ++ + K   +++++VIEECK FY AG ET+S+ LTWT+
Sbjct: 284  QAEGDNLLGLLLLSSSQNNLPENVMSTKDNAITLEEVIEECKQFYLAGHETTSSWLTWTV 343

Query: 108  IV 103
             V
Sbjct: 344  TV 345



 Score = 58.9 bits (141), Expect(2) = 1e-98
 Identities = 26/34 (76%), Positives = 29/34 (85%)
 Frame = -2

Query: 103 LAMHPSWQEKAREEVLQICGKNMPSYEYLNHLKI 2
           LAMHP+WQEKAREEV+QICGK  P  E L+HLKI
Sbjct: 346 LAMHPNWQEKAREEVMQICGKKEPDSEALSHLKI 379


>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  323 bits (829), Expect(2) = 2e-95
 Identities = 158/301 (52%), Positives = 222/301 (73%)
 Frame = -3

Query: 1005 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 826
            IKG  YKFL G+LK++    +EA +KP+ L+H IVPRV+PF+++TV+ YGK+SF W G  
Sbjct: 45   IKGPSYKFLSGNLKDIVSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPY 104

Query: 825  PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 646
              + I+DP++++++LS+K G  +K   NP  KLL +G+ S +GEKWVK R++++PAFH E
Sbjct: 105  CRVNIMDPDMIRDILSNKFGHFEKIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLE 164

Query: 645  KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 466
            KLK M PA  TSC E++ KW+    SQ  CELD W  +Q+ TADVISRTAFGS++EEG++
Sbjct: 165  KLKMMLPAIYTSCSEMVNKWEMMV-SQDSCELDVWPHLQNLTADVISRTAFGSSYEEGRR 223

Query: 465  IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 286
            IF+LQ EQ+ LV++A+QS+YIPG RF+PTK+N R   +  E  V LRD++ ++  A++ G
Sbjct: 224  IFQLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIG 283

Query: 285  KSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQETSSNLLTWTMI 106
            ++  DDLLG+L++ S      D  N K  GM+ID+VI+ECKLFYFAGQET+S LL WTM+
Sbjct: 284  EAHNDDLLGLLME-SNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMV 342

Query: 105  V 103
            V
Sbjct: 343  V 343



 Score = 52.8 bits (125), Expect(2) = 2e-95
 Identities = 23/34 (67%), Positives = 27/34 (79%)
 Frame = -2

Query: 103 LAMHPSWQEKAREEVLQICGKNMPSYEYLNHLKI 2
           L+MHP WQEKAREEVLQ+ G   P ++ LNHLKI
Sbjct: 344 LSMHPDWQEKAREEVLQVFGGKDPDFDGLNHLKI 377


>emb|CBI33076.3| unnamed protein product [Vitis vinifera]
          Length = 557

 Score =  315 bits (808), Expect(2) = 8e-94
 Identities = 158/301 (52%), Positives = 210/301 (69%)
 Frame = -3

Query: 1005 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 826
            I+GT YK L+GD K +   F EA SKP+ L H IVPRVIPF +   + YGK+S  W  T 
Sbjct: 90   IRGTHYKLLFGDAKAMKQSFMEARSKPMALNHSIVPRVIPFYHEMAQKYGKVSVSWHFTT 149

Query: 825  PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 646
            P ++I++PEL++ +L  K+G + + P NP    LS GL SL+GEKW K+RKL+SPAFH E
Sbjct: 150  PRVLIVEPELMRMILKYKNGHLHRLPGNPLGYHLSRGLLSLEGEKWAKRRKLLSPAFHLE 209

Query: 645  KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 466
            KLKGM PAF TSC +LIE+WK     QG  ELD   + Q+ T DVISRTAFGS++EEG++
Sbjct: 210  KLKGMMPAFSTSCHDLIERWKNLVGPQGTYELDVMPEFQNLTGDVISRTAFGSSYEEGRR 269

Query: 465  IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 286
            +FELQKEQ +LVM+  ++ YIPGFRF+PT+KNKRR  +D EI   ++ +I +K   L++G
Sbjct: 270  VFELQKEQIVLVMEDFRNFYIPGFRFVPTRKNKRRYYMDSEIKAMIKKIILKKKQTLKNG 329

Query: 285  KSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQETSSNLLTWTMI 106
                DDLLG+LLQ   ++          + M+ID V+EECKLFYF GQET++N LTWT +
Sbjct: 330  DPGNDDLLGLLLQCQEQTD---------SEMTIDDVVEECKLFYFVGQETTANWLTWTTL 380

Query: 105  V 103
            +
Sbjct: 381  L 381



 Score = 55.8 bits (133), Expect(2) = 8e-94
 Identities = 24/34 (70%), Positives = 29/34 (85%)
 Frame = -2

Query: 103 LAMHPSWQEKAREEVLQICGKNMPSYEYLNHLKI 2
           L+MHP+WQEKAR EVLQICGK MP  E +++LKI
Sbjct: 382 LSMHPNWQEKARAEVLQICGKKMPDIEAISNLKI 415


>ref|XP_003635296.1| PREDICTED: secologanin synthase-like [Vitis vinifera]
          Length = 511

 Score =  315 bits (808), Expect(2) = 8e-94
 Identities = 158/301 (52%), Positives = 210/301 (69%)
 Frame = -3

Query: 1005 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 826
            I+GT YK L+GD K +   F EA SKP+ L H IVPRVIPF +   + YGK+S  W  T 
Sbjct: 44   IRGTHYKLLFGDAKAMKQSFMEARSKPMALNHSIVPRVIPFYHEMAQKYGKVSVSWHFTT 103

Query: 825  PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 646
            P ++I++PEL++ +L  K+G + + P NP    LS GL SL+GEKW K+RKL+SPAFH E
Sbjct: 104  PRVLIVEPELMRMILKYKNGHLHRLPGNPLGYHLSRGLLSLEGEKWAKRRKLLSPAFHLE 163

Query: 645  KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 466
            KLKGM PAF TSC +LIE+WK     QG  ELD   + Q+ T DVISRTAFGS++EEG++
Sbjct: 164  KLKGMMPAFSTSCHDLIERWKNLVGPQGTYELDVMPEFQNLTGDVISRTAFGSSYEEGRR 223

Query: 465  IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 286
            +FELQKEQ +LVM+  ++ YIPGFRF+PT+KNKRR  +D EI   ++ +I +K   L++G
Sbjct: 224  VFELQKEQIVLVMEDFRNFYIPGFRFVPTRKNKRRYYMDSEIKAMIKKIILKKKQTLKNG 283

Query: 285  KSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQETSSNLLTWTMI 106
                DDLLG+LLQ   ++          + M+ID V+EECKLFYF GQET++N LTWT +
Sbjct: 284  DPGNDDLLGLLLQCQEQTD---------SEMTIDDVVEECKLFYFVGQETTANWLTWTTL 334

Query: 105  V 103
            +
Sbjct: 335  L 335



 Score = 55.8 bits (133), Expect(2) = 8e-94
 Identities = 24/34 (70%), Positives = 29/34 (85%)
 Frame = -2

Query: 103 LAMHPSWQEKAREEVLQICGKNMPSYEYLNHLKI 2
           L+MHP+WQEKAR EVLQICGK MP  E +++LKI
Sbjct: 336 LSMHPNWQEKARAEVLQICGKKMPDIEAISNLKI 369


>ref|XP_002516130.1| cytochrome P450, putative [Ricinus communis]
            gi|223544616|gb|EEF46132.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 510

 Score =  315 bits (807), Expect(2) = 9e-92
 Identities = 158/301 (52%), Positives = 209/301 (69%)
 Frame = -3

Query: 1005 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 826
            I+G  YK   GD + +      A SKPI L   I PRV+PF +  VK YGK+S  W GT+
Sbjct: 44   IRGNSYKLFNGDGEAITKSSMRALSKPIALNDQINPRVLPFFHEMVKNYGKVSLSWFGTR 103

Query: 825  PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 646
            P LI+ DPE+++ VL+DK+G   KPP NP + LL  G+S+L+G+KW K+RKL++PAFH E
Sbjct: 104  PRLILADPEMIRWVLTDKNGHFVKPPLNPLVNLLQLGVSTLEGDKWAKRRKLMTPAFHYE 163

Query: 645  KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 466
            KLK M P F TSC +LI +WKK    +G CE+D   +      DVI+RTAFGS+++EGK+
Sbjct: 164  KLKCMVPQFATSCSDLINRWKKLVSPKGLCEIDVATEFDALAGDVIARTAFGSSYQEGKR 223

Query: 465  IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 286
            IFELQKEQ  LV++A +S+YIPG RFIPTKKNKRR ++D EI   LRD+IRRK  A++  
Sbjct: 224  IFELQKEQVSLVLEAYRSIYIPGLRFIPTKKNKRRYDIDDEIKATLRDMIRRKEQAMQID 283

Query: 285  KSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQETSSNLLTWTMI 106
              S  DLL +L++  RE++ +DM N        + +IEECKL YFAGQET++N LTWT+I
Sbjct: 284  SPSNVDLLSLLIRCKREAA-SDMTN--------EDIIEECKLLYFAGQETTANWLTWTLI 334

Query: 105  V 103
            V
Sbjct: 335  V 335



 Score = 49.3 bits (116), Expect(2) = 9e-92
 Identities = 22/33 (66%), Positives = 26/33 (78%)
 Frame = -2

Query: 103 LAMHPSWQEKAREEVLQICGKNMPSYEYLNHLK 5
           L+ +P+WQ KAREEVLQICGK +P  E LN LK
Sbjct: 336 LSRNPNWQVKAREEVLQICGKKIPEIEDLNRLK 368