BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00022572
         (2416 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptas...    66   2e-21
gb|ABN09154.1| RNA-directed DNA polymerase (Reverse transcriptas...    71   1e-09
gb|ABD28670.2| RNA-directed DNA polymerase (Reverse transcriptas...    68   1e-08

>gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptase); Ribonuclease H;
            Endonuclease/exonuclease/phosphatase [Medicago
            truncatula]
          Length = 1246

 Score = 65.9 bits (159), Expect(2) = 2e-21
 Identities = 54/166 (32%), Positives = 83/166 (50%), Gaps = 18/166 (10%)
 Frame = +1

Query: 619  IPSYKEIETTVFAMD-QGNSGPDGFQGMFYQAYWNIVKSDVRKAIKSFF----------- 762
            +P + E++  VF ++  G  GP+GF G FYQ YW+IV +DV ++++ FF           
Sbjct: 441  LPLWGEVKNAVFTLNGDGAPGPNGFGGHFYQTYWDIVGADVIQSVQDFFISGQLAQNINS 500

Query: 763  --IVVIPIGMDSHFITHIR---MGNFIMN-*R*F*KNYK*DVTMLLRS*CQRNRQVSSKG 924
              IV+IP    +  +   R   + NF          +   D+TM + S  QR        
Sbjct: 501  NLIVLIPKVPGARVMGDYRPIALANFQFKIISKILADRLADITMRIISVEQRG--FIRDR 558

Query: 925  DASKAFQANIGLHSGMANELKKKIFAGNVGLRIDISQAYDTLSW*F 1062
            D SK     + L S   N L+K+ + GNV L++DI++A+DTL W F
Sbjct: 559  DISKC----VILASEAINLLEKRQYGGNVALKVDIAKAFDTLDWNF 600



 Score = 65.5 bits (158), Expect(2) = 2e-21
 Identities = 43/136 (31%), Positives = 67/136 (49%), Gaps = 2/136 (1%)
 Frame = +2

Query: 1076 TISHGE*RS--MRSLLFAPAEDVLSRGLNFLREQGLXXXXXXXXXXXXXGPSHLLFADDI 1249
            T SHG  +   +  LLF   E+VLSR L+     G               P+H+L+ADD+
Sbjct: 640  TCSHGVRQGDPLSPLLFCLVEEVLSRALSMAATDGQLIPMSYCRGVSF--PTHILYADDV 697

Query: 1250 FIFAKGDLRGVRYIAGLLHRCELSSGQCVSKAKSKLFTGCVTVVRKELLQNELGITVGAF 1429
             IF  G  R +R +  +  +    SGQ ++ AKS+ FT  +T  R +++ + LG  VG+ 
Sbjct: 698  LIFCTGTKRNIRRLIKIFSQYSEVSGQLINNAKSRFFTSAMTGSRVQMISSLLGFNVGSL 757

Query: 1430 LERDLGSWVYPLFKAR 1477
                LG    P+F+ +
Sbjct: 758  PFTYLGC---PIFRGK 770


>gb|ABN09154.1| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
            truncatula]
          Length = 528

 Score = 71.2 bits (173), Expect = 1e-09
 Identities = 55/172 (31%), Positives = 81/172 (47%), Gaps = 21/172 (12%)
 Frame = +1

Query: 610  IAAIPSYKEIETTVFAMDQGNS-GPDGFQGMFYQAYWNIVKSDVRKAIKSFF-------- 762
            +  IPS  EI+  VF+++  ++ GPDGF   FYQ YW+IVK DV KA+  FF        
Sbjct: 112  LTMIPSNDEIKQAVFSLNNDSAPGPDGFGSCFYQIYWDIVKEDVIKAVLQFFNTGWILPN 171

Query: 763  -----IVVIPIGMDSHFITHIR---MGNFIMN*R*F*KNYK*DVTMLLRS*CQRNRQVSS 918
                 +++IP   ++  +   R   M NF          +K    +L     Q    + S
Sbjct: 172  FNANTLILIPKTQNADSMDQFRPIAMANF---------KFKIISKILADRLAQIMPNIVS 222

Query: 919  KGDASKAFQANIG----LHSGMANELKKKIFAGNVGLRIDISQAYDTLSW*F 1062
            +         NI     L S   N L +K F GN+  ++DIS+A+DTL+W F
Sbjct: 223  QEQRGFIQGRNIKDCVCLASEAINMLDQKSFGGNLAFKVDISKAFDTLNWKF 274


>gb|ABD28670.2| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
            truncatula]
          Length = 642

 Score = 67.8 bits (164), Expect = 1e-08
 Identities = 51/173 (29%), Positives = 88/173 (50%), Gaps = 20/173 (11%)
 Frame = +1

Query: 604  KMIAAIPSYKEIETTVFAMDQGNS-GPDGFQGMFYQAYWNIVKSDVRKAIKSFF------ 762
            +++  +P+ +E++  VF ++  ++ GPD F   F+Q YWNIVK DV +A+  FF      
Sbjct: 171  RLLTMLPTKEEVKNAVFDLNSDDAPGPDVFGACFFQIYWNIVKKDVYEAVLDFFKNGWLP 230

Query: 763  -------IVVIPIGMDSHFITHIR---MGNF---IMN*R*F*KNYK*DVTMLLRS*CQRN 903
                   I++IP   ++  +   R   + NF   I+N     K     +  +L S   + 
Sbjct: 231  NNFNANSIILIPKTPNADSVDQYRTIALVNFKFKIIN-----KVLADRLAKILPSIISKE 285

Query: 904  RQVSSKGDASKAFQANIGLHSGMANELKKKIFAGNVGLRIDISQAYDTLSW*F 1062
            ++   +G   +  +  I L S   N L  K F GN+ L+ID+++A+DTL+W F
Sbjct: 286  QRGFVQG---RNIRDCIALTSEAINVLDNKSFGGNLALKIDVTKAFDTLNWDF 335



 Score = 57.4 bits (137), Expect(2) = 4e-07
 Identities = 38/122 (31%), Positives = 58/122 (47%)
 Frame = +2

Query: 1112 LLFAPAEDVLSRGLNFLREQGLXXXXXXXXXXXXXGPSHLLFADDIFIFAKGDLRGVRYI 1291
            LLF   E+VLSR ++ L ++GL              P H  + DD+ +F K  +  +  +
Sbjct: 389  LLFCIVEEVLSRSISILADKGLIDLIAASRNNCL--PFHCFYVDDLMVFCKAKMSSLIVL 446

Query: 1292 AGLLHRCELSSGQCVSKAKSKLFTGCVTVVRKELLQNELGITVGAFLERDLGSWVYPLFK 1471
              L  R    SGQ ++  KS +F G +T  R   + N LG  VG+     LG+   P+FK
Sbjct: 447  KSLFTRYADCSGQIMNIRKSFIFAGGITDTRMNNIVNILGFNVGSLPFTYLGA---PIFK 503

Query: 1472 AR 1477
             +
Sbjct: 504  GK 505



 Score = 25.4 bits (54), Expect(2) = 4e-07
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = +1

Query: 1450 LGVPIVQGKIKNAEVRIVVDKIRKWTIGLKITLIS 1554
            LG PI +GK K    + + DK++      K +L+S
Sbjct: 497  LGAPIFKGKPKGIHFQPIADKVKAKLAKWKASLLS 531