BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00022508
         (1899 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis v...   897   0.0  
emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]   895   0.0  
sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=A...   860   0.0  
ref|XP_002530197.1| l-ascorbate oxidase, putative [Ricinus commu...   843   0.0  
ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trich...   843   0.0  

>ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis vinifera]
          Length = 581

 Score =  897 bits (2318), Expect = 0.0
 Identities = 416/559 (74%), Positives = 472/559 (84%), Gaps = 2/559 (0%)
 Frame = -2

Query: 1727 QLSLAAKTRHFRWEVSYLFWSPDCVENVVMGINGQYPGPTIRAKAGDTIVVHLHNKLHTE 1548
            QL LA+KTRHF+WEV Y++WSPDC+E VVMGINGQ+PGPTIRA AGDTIVV L N+LHTE
Sbjct: 24   QLCLASKTRHFKWEVEYMYWSPDCMEGVVMGINGQFPGPTIRAVAGDTIVVELTNRLHTE 83

Query: 1547 GVVIHWHGIRQFETPWADGTASISQCVINPEETFIYRFKIDKAGTYFYHGHYGMQRSAGL 1368
            GVVIHWHGIRQF TPWADGTASISQC INP ETFIYR+K+DKAGTYFYHGHYGMQRSAGL
Sbjct: 84   GVVIHWHGIRQFGTPWADGTASISQCAINPGETFIYRYKVDKAGTYFYHGHYGMQRSAGL 143

Query: 1367 YGSLIVDVADGEKEPFHYEGELNLLLSDWWHQNVHEQEIGLSSNPFRWIGEPQSLLMNGR 1188
            YGSL+V+V +G+KEPFHY+GE NLLLSDWWH+   EQE+ LSS PFRWIGEPQ+LL+NGR
Sbjct: 144  YGSLVVEVGEGQKEPFHYDGEFNLLLSDWWHKGSQEQEVALSSKPFRWIGEPQTLLINGR 203

Query: 1187 GQFNCSLAAHLSNSSV--CNIRGGEQCAPPILHVQPNKTYRLRIASTTSLASLNLAIGHH 1014
            GQ+NCSLAAH++NSS   C  RG EQC+P ILHVQP+KTYRLR++STT+LASLNL IG+H
Sbjct: 204  GQYNCSLAAHVTNSSSPQCQFRGNEQCSPQILHVQPHKTYRLRVSSTTALASLNLQIGNH 263

Query: 1013 KMVLVEADGNYVQPVTIDNMDIYSGESYSVLIHTDQNPSMNYWVSVNVXXXXXXXXXXXX 834
            KMV+VEADGNY+QPV +D++DIYSGESYSVLIHTDQ+PS NYW+SV+V            
Sbjct: 264  KMVMVEADGNYIQPVAVDDLDIYSGESYSVLIHTDQDPSKNYWISVSVRGREPKTPQGLT 323

Query: 833  XLNYHPNSASKLPASPLPIPPAWNDYVHSKSFSNKILALQGTPKPPMRYDRRIYLLNTQN 654
             LNY   SASKLP S  P+ P WNDY HSKSFSNKILAL G+PKPP  Y+RRI LLNTQN
Sbjct: 324  ILNYQTTSASKLPTSTPPVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQN 383

Query: 653  RINGYTKWAINNISLTLPSTPYLGSIKFGLKGAFDHQKPPEEFGSNYNVMKPPVNPNSTY 474
             ING+TKWAINNISLTLP TPYLG+IK  L  AFD + PPE F ++Y+VMKPP N NSTY
Sbjct: 384  TINGFTKWAINNISLTLPPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTY 443

Query: 473  GSGVYTLAFNRTIDIILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFSEEKDSDKLN 294
            G+GVY L F  T+D+ILQNANALA  VSEIHPWHLHGHDFWVLGYGEGKF  EKD+ + N
Sbjct: 444  GNGVYMLEFRTTVDVILQNANALATGVSEIHPWHLHGHDFWVLGYGEGKF-REKDAKRFN 502

Query: 293  LKNPPLRNTVVIFPYGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVERIKKKIP 114
            LKNPPLRNT VIFP+GWTALRFV DNPGVWAFHCHIEPHLHMGMGV+FAEGV  + K +P
Sbjct: 503  LKNPPLRNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLV-KDVP 561

Query: 113  NAAIGCGMTAKKLMDDNRH 57
            N A+ CG+T K LM +  H
Sbjct: 562  NHALACGLTGKMLMSNYSH 580


>emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]
          Length = 558

 Score =  895 bits (2312), Expect = 0.0
 Identities = 416/558 (74%), Positives = 470/558 (84%), Gaps = 2/558 (0%)
 Frame = -2

Query: 1724 LSLAAKTRHFRWEVSYLFWSPDCVENVVMGINGQYPGPTIRAKAGDTIVVHLHNKLHTEG 1545
            L LA+KTRHF+WEV Y++WSPDC+E VVMGINGQ+PGPTIRA AGDTIVV L N+LHTEG
Sbjct: 2    LCLASKTRHFKWEVEYMYWSPDCMEGVVMGINGQFPGPTIRAVAGDTIVVELTNRLHTEG 61

Query: 1544 VVIHWHGIRQFETPWADGTASISQCVINPEETFIYRFKIDKAGTYFYHGHYGMQRSAGLY 1365
            VVIHWHGIRQF TPWADGTASISQC INP ETFIYR+K+DKAGTYFYHGHYGMQRSAGLY
Sbjct: 62   VVIHWHGIRQFGTPWADGTASISQCAINPGETFIYRYKVDKAGTYFYHGHYGMQRSAGLY 121

Query: 1364 GSLIVDVADGEKEPFHYEGELNLLLSDWWHQNVHEQEIGLSSNPFRWIGEPQSLLMNGRG 1185
            GSL V+V +G+KEPFHY+GE NLLLSDWWH+   EQE+ LSS PFRWIGEPQ+LL+NGRG
Sbjct: 122  GSLXVEVGEGQKEPFHYDGEFNLLLSDWWHKGSQEQEVALSSKPFRWIGEPQTLLINGRG 181

Query: 1184 QFNCSLAAHLSNSSV--CNIRGGEQCAPPILHVQPNKTYRLRIASTTSLASLNLAIGHHK 1011
            Q+NCSLAAH++NSS   C  RG EQCAP ILHVQP+KTYRLR++STT+LASLNL IG+HK
Sbjct: 182  QYNCSLAAHVTNSSSPQCQFRGNEQCAPQILHVQPHKTYRLRVSSTTALASLNLQIGNHK 241

Query: 1010 MVLVEADGNYVQPVTIDNMDIYSGESYSVLIHTDQNPSMNYWVSVNVXXXXXXXXXXXXX 831
            MV+VEADGNY+QPV +D++DIYSGESYSVLIHTDQ+PS NYW+SV+V             
Sbjct: 242  MVMVEADGNYIQPVAVDDLDIYSGESYSVLIHTDQDPSXNYWISVSVRGREPKTPQGLTI 301

Query: 830  LNYHPNSASKLPASPLPIPPAWNDYVHSKSFSNKILALQGTPKPPMRYDRRIYLLNTQNR 651
            LNY   SASKLP S  P+ P WNDY HSKSFSNKILAL G+PKPP  Y+RRI LLNTQN 
Sbjct: 302  LNYQTTSASKLPTSTPPVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNT 361

Query: 650  INGYTKWAINNISLTLPSTPYLGSIKFGLKGAFDHQKPPEEFGSNYNVMKPPVNPNSTYG 471
            ING+TKWAINNISLTLP TPYLG+IK  L  AFD + PPE F ++Y+VMKPP N NSTYG
Sbjct: 362  INGFTKWAINNISLTLPPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYG 421

Query: 470  SGVYTLAFNRTIDIILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFSEEKDSDKLNL 291
            +GVY L F  T+D+ILQNANALA  VSEIHPWHLHGHDFWVLGYGEGKF  EKD+ + NL
Sbjct: 422  NGVYMLEFRTTVDVILQNANALATGVSEIHPWHLHGHDFWVLGYGEGKF-REKDAKRFNL 480

Query: 290  KNPPLRNTVVIFPYGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVERIKKKIPN 111
            KNPPLRNT VIFP+GWTALRFV DNPGVWAFHCHIEPHLHMGMGV+FAEGV  + K +PN
Sbjct: 481  KNPPLRNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLV-KDVPN 539

Query: 110  AAIGCGMTAKKLMDDNRH 57
             A+ CG+T K LM +  H
Sbjct: 540  HALACGLTGKMLMSNYSH 557


>sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags:
            Precursor gi|599594|dbj|BAA07734.1| ascorbate oxidase
            precursor [Nicotiana tabacum]
          Length = 578

 Score =  860 bits (2222), Expect = 0.0
 Identities = 409/560 (73%), Positives = 464/560 (82%), Gaps = 2/560 (0%)
 Frame = -2

Query: 1730 SQLSLAAKTRHFRWEVSYLFWSPDCVENVVMGINGQYPGPTIRAKAGDTIVVHLHNKLHT 1551
            S+  +AAKTRHF+W+V Y+ WSPD  E+VVMGINGQ+PGPTIRAKAGDT+ VHL NKLHT
Sbjct: 22   SRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKAGDTVAVHLTNKLHT 81

Query: 1550 EGVVIHWHGIRQFETPWADGTASISQCVINPEETFIYRFKIDKAGTYFYHGHYGMQRSAG 1371
            EGVVIHWHGIRQ  TPWADGTA+ISQC INP ETF+YRFK+DKAGTYFYHGHYGMQRSAG
Sbjct: 82   EGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGTYFYHGHYGMQRSAG 141

Query: 1370 LYGSLIVDVADGEKEPFHYEGELNLLLSDWWHQNVHEQEIGLSSNPFRWIGEPQSLLMNG 1191
            LYGSLIV+V +GEKEPFHY+GE NLLLSDWWH+  HEQE+ LSSNP RWIGEPQ+LL+NG
Sbjct: 142  LYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSNPLRWIGEPQTLLLNG 201

Query: 1190 RGQFNCSLAAHLSNSSV--CNIRGGEQCAPPILHVQPNKTYRLRIASTTSLASLNLAIGH 1017
            RGQ+NCSLAA  S   +  C +RGGEQ AP IL V+PNK YRLR+ASTT+L SL+LAIG 
Sbjct: 202  RGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGG 261

Query: 1016 HKMVLVEADGNYVQPVTIDNMDIYSGESYSVLIHTDQNPSMNYWVSVNVXXXXXXXXXXX 837
            HKMV+VEADGNYVQP ++ +MDIYSGESYSVL  TDQ+P+ NYW+S+NV           
Sbjct: 262  HKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGL 321

Query: 836  XXLNYHPNSASKLPASPLPIPPAWNDYVHSKSFSNKILALQGTPKPPMRYDRRIYLLNTQ 657
              LNY PNSASK P  P PI P WNDY HSKSFSNKI AL G+PKPP +  RRI LLNTQ
Sbjct: 322  TLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQNHRRIILLNTQ 381

Query: 656  NRINGYTKWAINNISLTLPSTPYLGSIKFGLKGAFDHQKPPEEFGSNYNVMKPPVNPNST 477
            N+I+GYTKWAINN+SL LP+  YLGSI++G+  AFD + PP+ F  +Y+V+K   N NST
Sbjct: 382  NKIDGYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDNFPKDYDVLKQAPNSNST 440

Query: 476  YGSGVYTLAFNRTIDIILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFSEEKDSDKL 297
            YG+GVY L FN TIDIILQNANALA++VSEIHPWHLHGHDFWVLGYGEGKFS EKD  K 
Sbjct: 441  YGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFS-EKDVKKF 499

Query: 296  NLKNPPLRNTVVIFPYGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVERIKKKI 117
            NLKNPPLRNT VIFP+GWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGV  + KKI
Sbjct: 500  NLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLV-KKI 558

Query: 116  PNAAIGCGMTAKKLMDDNRH 57
            P  A+ CG+T K LM  N+H
Sbjct: 559  PKEALACGLTGKMLM-SNKH 577


>ref|XP_002530197.1| l-ascorbate oxidase, putative [Ricinus communis]
            gi|223530290|gb|EEF32187.1| l-ascorbate oxidase, putative
            [Ricinus communis]
          Length = 589

 Score =  843 bits (2177), Expect = 0.0
 Identities = 387/552 (70%), Positives = 457/552 (82%), Gaps = 3/552 (0%)
 Frame = -2

Query: 1703 RHF-RWEVSYLFWSPDCVENVVMGINGQYPGPTIRAKAGDTIVVHLHNKLHTEGVVIHWH 1527
            RHF +W+V Y+FWSPDC ENVVMGINGQ+PGPTIRA AGD I + L NKLHTEGVVIHWH
Sbjct: 40   RHFYKWDVEYMFWSPDCEENVVMGINGQFPGPTIRAHAGDYIHIELTNKLHTEGVVIHWH 99

Query: 1526 GIRQFETPWADGTASISQCVINPEETFIYRFKIDKAGTYFYHGHYGMQRSAGLYGSLIVD 1347
            GIRQ  TPWADGTA+ISQC INP ETF YRF +D+ GTYFYHGHYGMQRSAGLYGSLIVD
Sbjct: 100  GIRQLGTPWADGTAAISQCAINPGETFTYRFIVDRPGTYFYHGHYGMQRSAGLYGSLIVD 159

Query: 1346 VADGEKEPFHYEGELNLLLSDWWHQNVHEQEIGLSSNPFRWIGEPQSLLMNGRGQFNCSL 1167
            VA+G+KEPF Y+GE +LLLSDWWH+NVH QE+GLSS+P RWIGEPQ++LMNGRGQ+NCSL
Sbjct: 160  VAEGQKEPFKYDGEFDLLLSDWWHENVHHQEVGLSSSPMRWIGEPQTILMNGRGQYNCSL 219

Query: 1166 AAHLSNSSV--CNIRGGEQCAPPILHVQPNKTYRLRIASTTSLASLNLAIGHHKMVLVEA 993
            AA  S +    C ++GGE CAP I HV+P K YRLRIASTT+LASLNLAI  HKM++VEA
Sbjct: 220  AAVYSRNPTQQCKLKGGENCAPFIFHVEPKKKYRLRIASTTALASLNLAIDGHKMLVVEA 279

Query: 992  DGNYVQPVTIDNMDIYSGESYSVLIHTDQNPSMNYWVSVNVXXXXXXXXXXXXXLNYHPN 813
            DGNYV+P   D++DIYSGESYSV+I T+Q+P  NYW+S  V             L+Y+  
Sbjct: 280  DGNYVKPFETDSLDIYSGESYSVIITTNQHPYQNYWISAGVIGREPHTPQGLTLLHYNQT 339

Query: 812  SASKLPASPLPIPPAWNDYVHSKSFSNKILALQGTPKPPMRYDRRIYLLNTQNRINGYTK 633
            SASKLP SP P+ P WND   SK+F+NKILA +GT  PP  YDRRI LLNTQN+ NG+TK
Sbjct: 340  SASKLPESPPPVTPRWNDTARSKAFTNKILAKKGTQPPPKNYDRRIILLNTQNKYNGHTK 399

Query: 632  WAINNISLTLPSTPYLGSIKFGLKGAFDHQKPPEEFGSNYNVMKPPVNPNSTYGSGVYTL 453
            WAINN+SL+LP+TPYLG++KFGL+ AFD +KPPE++  +Y+VMKP  NPN+T GSGVYT+
Sbjct: 400  WAINNVSLSLPATPYLGAMKFGLRNAFDQEKPPEKYDESYDVMKPAANPNTTVGSGVYTI 459

Query: 452  AFNRTIDIILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFSEEKDSDKLNLKNPPLR 273
             +  T+D+ILQNANAL+++VSE+HPWHLHGHDFWVLGYGEGKF+ EKD  K NLKNPPLR
Sbjct: 460  GYQTTVDVILQNANALSKDVSEVHPWHLHGHDFWVLGYGEGKFT-EKDEKKFNLKNPPLR 518

Query: 272  NTVVIFPYGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVERIKKKIPNAAIGCG 93
            NT VIFPYGWTALRFV DNPGVWAFHCHIEPHLHMGMGV+FAEG+E + K IP+ A+ CG
Sbjct: 519  NTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGIEHV-KDIPHEALACG 577

Query: 92   MTAKKLMDDNRH 57
            +T ++ MD N+H
Sbjct: 578  LTGRRFMDTNKH 589


>ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trichocarpa]
            gi|222849246|gb|EEE86793.1| l-ascorbate oxidase precursor
            [Populus trichocarpa]
          Length = 597

 Score =  843 bits (2177), Expect = 0.0
 Identities = 394/570 (69%), Positives = 464/570 (81%), Gaps = 12/570 (2%)
 Frame = -2

Query: 1736 AFSQLSLAAKTRH-------FRWEVSYLFWSPDCVENVVMGINGQYPGPTIRAKAGDTIV 1578
            + +Q SLA  T +       ++WEV Y++WSPD +ENVVMGING++PGPTIRA+AGDT+ 
Sbjct: 27   SLTQCSLATATSNKTRSLHFYKWEVEYMYWSPDGLENVVMGINGKFPGPTIRARAGDTVH 86

Query: 1577 VHLHNKLHTEGVVIHWHGIRQFETPWADGTASISQCVINPEETFIYRFKIDKAGTYFYHG 1398
            VHL NKLHTEGVVIHWHGIRQ  TPWADGTASISQC INP E+F YRF +D+AGTYFYHG
Sbjct: 87   VHLTNKLHTEGVVIHWHGIRQKGTPWADGTASISQCAINPGESFDYRFTVDRAGTYFYHG 146

Query: 1397 HYGMQRSAGLYGSLIVDVADGEKEPFHYEGELNLLLSDWWHQNVHEQEIGLSSNPFRWIG 1218
            HYGMQRSAGLYGSLIVDVA+GEKEPFHY+GE +LLLSDWWH++ H QE+GLSS P RWIG
Sbjct: 147  HYGMQRSAGLYGSLIVDVAEGEKEPFHYDGEFDLLLSDWWHESAHHQEVGLSSRPMRWIG 206

Query: 1217 EPQSLLMNGRGQFNCSLAAHLSNS---SVCNIRGGEQCAPPILHVQPNKTYRLRIASTTS 1047
            EPQ+LL+NGRGQ+ CSLAAH SN+   S CN+ G EQ AP ILHV PNKTYR+R++STT+
Sbjct: 207  EPQTLLVNGRGQYGCSLAAHYSNNSSLSQCNVTGHEQWAPYILHVDPNKTYRIRLSSTTA 266

Query: 1046 LASLNLAIGHHKMVLVEADGNYVQPVTIDNMDIYSGESYSVLIHTDQNPSMNYWVSVNVX 867
            LASLNLAIG+HKM++VEADGNY+QP   D++DIYSGESYSVL+ T Q+PS NYW+S  V 
Sbjct: 267  LASLNLAIGNHKMLVVEADGNYLQPFETDDLDIYSGESYSVLLKTSQDPSQNYWISFGVR 326

Query: 866  XXXXXXXXXXXXLNYHPNSASKLPASPLPIPPAWNDYVHSKSFSNKILAL--QGTPKPPM 693
                        LNY  NSASK P SP P+ P W+DY HSK+F+NK+ AL  +  PKPP 
Sbjct: 327  GRKPQTPQALTILNYKTNSASKFPLSPPPVTPRWDDYAHSKAFTNKVKALDHKTIPKPPS 386

Query: 692  RYDRRIYLLNTQNRINGYTKWAINNISLTLPSTPYLGSIKFGLKGAFDHQKPPEEFGSNY 513
             Y RRI LLNTQN++NGYTKW+INN+SL+LP+TPYLGSI+FGL+  FD  KPPE F   Y
Sbjct: 387  TYHRRIILLNTQNKMNGYTKWSINNVSLSLPATPYLGSIRFGLQNGFDQTKPPESFPVQY 446

Query: 512  NVMKPPVNPNSTYGSGVYTLAFNRTIDIILQNANALAENVSEIHPWHLHGHDFWVLGYGE 333
            +VMKPP NPN+T G+GVY L++  T+D+ILQNANALAENVSEIHPWHLHGHDFWVLGYGE
Sbjct: 447  DVMKPPGNPNTTTGNGVYMLSYYSTVDVILQNANALAENVSEIHPWHLHGHDFWVLGYGE 506

Query: 332  GKFSEEKDSDKLNLKNPPLRNTVVIFPYGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVI 153
            GKF+++ D  K N+KNPP RN+ VIFPYGWTALRFV DNPGVWAFHCHIEPHLHMGMGV+
Sbjct: 507  GKFTKD-DEKKFNMKNPPYRNSAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVV 565

Query: 152  FAEGVERIKKKIPNAAIGCGMTAKKLMDDN 63
             AEGV+R+  KIP  A+ CG+T KK M  N
Sbjct: 566  LAEGVQRL-PKIPKEALSCGLTGKKFMTGN 594