BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00022310
         (820 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAR83909.1| heavy metal transporter MTP2 [Arabidopsis lyrata]      225   1e-58
ref|XP_002320234.1| metal tolerance protein [Populus trichocarpa...   220   8e-57
gb|AAO83660.1| cation-efflux transporter [Brassica juncea]            218   2e-56
ref|XP_002876481.1| metal tolerance protein A2 [Arabidopsis lyra...   219   3e-56
ref|XP_002298977.1| metal tolerance protein [Populus trichocarpa...   218   4e-56

>gb|AAR83909.1| heavy metal transporter MTP2 [Arabidopsis lyrata]
          Length = 364

 Score =  225 bits (573), Expect(2) = 1e-58
 Identities = 119/207 (57%), Positives = 150/207 (72%), Gaps = 16/207 (7%)
 Frame = -2

Query: 771 VYEAICRLINHTAHEVKGYLMFIVAAIGLGVNIVMAVVLCHNH--SRGHSNDNR------ 616
           VYEAI +++  T+ EV G+LMF+VAA GL VNI+MAV+L H+H  S GH  D        
Sbjct: 143 VYEAIIKIVTETS-EVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGEDKHHAHGDV 201

Query: 615 -DSTREANNTEVVNSEKRHWNISVQGVYLHVIGDSIQSIGAMIA*AILWHKPELKIIDLL 439
            +   + + T+V   EKR  NI+VQG YLHV+GDSIQS+G MI  AI+W+ PE KI+DL+
Sbjct: 202 TEQLLDKSKTQVAAKEKRKRNINVQGAYLHVLGDSIQSVGVMIGGAIIWYNPEWKIVDLI 261

Query: 438 CTFTFSVIVLGTTIEMLSNIFEVLMESTPREIDVSRIEKGLCEMHEVVAIHELHIWAITV 259
           CT  FSVIVLGTTI M+ NI EVLMESTPREID ++++KGL EM EVVA+HELHIWAITV
Sbjct: 262 CTLVFSVIVLGTTINMIRNILEVLMESTPREIDATKLKKGLLEMEEVVAVHELHIWAITV 321

Query: 258 GKILLA-------DACTDIVL*NMVDY 199
           GK+LLA       +A  D+VL  ++DY
Sbjct: 322 GKVLLACHVNIRPEADADMVLNKVIDY 348



 Score = 28.1 bits (61), Expect(2) = 1e-58
 Identities = 12/14 (85%), Positives = 13/14 (92%)
 Frame = -3

Query: 188 YIRRECNISHVTMQ 147
           YIRRE NISHVT+Q
Sbjct: 348 YIRREYNISHVTIQ 361


>ref|XP_002320234.1| metal tolerance protein [Populus trichocarpa]
           gi|222861007|gb|EEE98549.1| metal tolerance protein
           [Populus trichocarpa]
          Length = 368

 Score =  220 bits (560), Expect(2) = 8e-57
 Identities = 125/214 (58%), Positives = 152/214 (71%), Gaps = 23/214 (10%)
 Frame = -2

Query: 771 VYEAICRLINHTAHEVKGYLMFIVAAIGLGVNIVMAVVLCHNHSRGHSNDNRDSTREANN 592
           VYE I RLI+ T+ EV G+LMF+VAA GL VNI+MA+VL H+H   H  D++  T  ++ 
Sbjct: 142 VYETIIRLIHGTS-EVNGFLMFLVAAFGLLVNIIMALVLGHDHGPDH--DHKHGTGHSHG 198

Query: 591 TEVVNS----------------EKRHWNISVQGVYLHVIGDSIQSIGAMIA*AILWHKPE 460
           T   ++                EK+  NI+VQG YLHV+GDSIQSIG MI  AI+W+KPE
Sbjct: 199 TTHHHAHEDHVEPLLDKGEAMHEKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPE 258

Query: 459 LKIIDLLCTFTFSVIVLGTTIEMLSNIFEVLMESTPREIDVSRIEKGLCEMHEVVAIHEL 280
            KIIDL+CT  FSVIVLGTTI+ML NI EVLMESTPREID ++IEKGL EM EV+AIHEL
Sbjct: 259 WKIIDLICTLIFSVIVLGTTIKMLRNILEVLMESTPREIDATKIEKGLLEMEEVMAIHEL 318

Query: 279 HIWAITVGKILLA-------DACTDIVL*NMVDY 199
           HIWAITVGKILLA       +A  D+VL N++ Y
Sbjct: 319 HIWAITVGKILLACHVKIMPEANADMVLDNVISY 352



 Score = 27.3 bits (59), Expect(2) = 8e-57
 Identities = 11/14 (78%), Positives = 13/14 (92%)
 Frame = -3

Query: 188 YIRRECNISHVTMQ 147
           Y+RRE NISHVT+Q
Sbjct: 352 YLRREYNISHVTIQ 365


>gb|AAO83660.1| cation-efflux transporter [Brassica juncea]
          Length = 376

 Score =  218 bits (554), Expect(2) = 2e-56
 Identities = 119/219 (54%), Positives = 148/219 (67%), Gaps = 28/219 (12%)
 Frame = -2

Query: 771 VYEAICRLINHTAHEVKGYLMFIVAAIGLGVNIVMAVVLCHNHSRGHSN----------- 625
           VYEAI RL+  T+ EV G+LMF+VAA GL VNIVMAV+L H+H  GH +           
Sbjct: 143 VYEAIIRLLTETS-EVDGFLMFLVAAFGLLVNIVMAVLLGHDHGHGHGHGHSHDGVTVTT 201

Query: 624 ----------DNRDSTREANNTEVVNSEKRHWNISVQGVYLHVIGDSIQSIGAMIA*AIL 475
                     D   S  +     +  SEKR  NI+VQG YLHV+GD IQS+G M+  AI+
Sbjct: 202 HHHHGHTHGEDKHHSHGDVTEELLDKSEKRKRNINVQGAYLHVLGDLIQSVGVMVGGAII 261

Query: 474 WHKPELKIIDLLCTFTFSVIVLGTTIEMLSNIFEVLMESTPREIDVSRIEKGLCEMHEVV 295
           W+KPE KI+DL+CT  FSVIVLGTTI M+ +I EVLMESTPREID +++E+GL EM EVV
Sbjct: 262 WYKPEWKIVDLICTLVFSVIVLGTTINMIRSILEVLMESTPREIDATKLEQGLLEMEEVV 321

Query: 294 AIHELHIWAITVGKILLA-------DACTDIVL*NMVDY 199
           A+HELHIWAITVGK+LLA       +A  D+VL  ++DY
Sbjct: 322 AVHELHIWAITVGKVLLACHVNITPEADADMVLNKVIDY 360



 Score = 28.1 bits (61), Expect(2) = 2e-56
 Identities = 12/14 (85%), Positives = 13/14 (92%)
 Frame = -3

Query: 188 YIRRECNISHVTMQ 147
           YIRRE NISHVT+Q
Sbjct: 360 YIRREYNISHVTIQ 373


>ref|XP_002876481.1| metal tolerance protein A2 [Arabidopsis lyrata subsp. lyrata]
           gi|297322319|gb|EFH52740.1| metal tolerance protein A2
           [Arabidopsis lyrata subsp. lyrata]
          Length = 379

 Score =  219 bits (557), Expect(2) = 3e-56
 Identities = 120/225 (53%), Positives = 155/225 (68%), Gaps = 29/225 (12%)
 Frame = -2

Query: 771 VYEAICRLINHTAHEVKGYLMFIVAAIGLGVNIVMAVVL-----------CHNHSRGHSN 625
           VYEAI RL N+ + EV+G LMF V+A+GL VNI MA++L            H+H  GHS+
Sbjct: 144 VYEAIVRL-NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGGHSHSHDHGHGHSH 202

Query: 624 DNRDSTR-----------EANNTEVVNSEKRHWNISVQGVYLHVIGDSIQSIGAMIA*AI 478
           D+                E++ +EV+  +K+  N+++QG YLHV+GDSIQS+G MI  AI
Sbjct: 203 DHDHGIAATEHHHGQRHDESHLSEVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAI 262

Query: 477 LWHKPELKIIDLLCTFTFSVIVLGTTIEMLSNIFEVLMESTPREIDVSRIEKGLCEMHEV 298
           +W+KPE KI+DL+CT  FSVIVLGTTIEML NI EVLMESTPREID + +EKG+CE+ EV
Sbjct: 263 IWYKPEWKILDLICTLVFSVIVLGTTIEMLRNILEVLMESTPREIDATMLEKGVCEIEEV 322

Query: 297 VAIHELHIWAITVGKILLA-------DACTDIVL*NMVDYYTSIH 184
           VA+HELHIWAITVGK+LLA       +A  D+VL  ++DY    H
Sbjct: 323 VAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREH 367



 Score = 26.6 bits (57), Expect(2) = 3e-56
 Identities = 11/14 (78%), Positives = 13/14 (92%)
 Frame = -3

Query: 188 YIRRECNISHVTMQ 147
           YI+RE NISHVT+Q
Sbjct: 362 YIKREHNISHVTIQ 375


>ref|XP_002298977.1| metal tolerance protein [Populus trichocarpa]
           gi|222846235|gb|EEE83782.1| metal tolerance protein
           [Populus trichocarpa]
          Length = 397

 Score =  218 bits (556), Expect(2) = 4e-56
 Identities = 128/245 (52%), Positives = 152/245 (62%), Gaps = 49/245 (20%)
 Frame = -2

Query: 771 VYEAICRLINHTAHEVKGYLMFIVAAIGLGVNIVMAVVLCHNHSRGH------------- 631
           VYEAI R+I+ T  EVKG LMF VAA+GL VNI MA +L H+H  GH             
Sbjct: 143 VYEAIVRIIHDTG-EVKGALMFAVAAVGLLVNIGMAFLLGHDHGHGHGHGHGHGHGHGHE 201

Query: 630 ------------SNDNRDS-----------------TREANNTEVVNSEKRHWNISVQGV 538
                       S++N D                  T   N T+  + +K+  NI+VQG 
Sbjct: 202 HATHHNHHHHEGSSENNDEHHHTHGADLAEPLLSTHTEVDNKTKGGSKQKKQRNINVQGA 261

Query: 537 YLHVIGDSIQSIGAMIA*AILWHKPELKIIDLLCTFTFSVIVLGTTIEMLSNIFEVLMES 358
           YLHV+GDSIQS+G MI  AI+W+KPE KIIDL+CT  FS+IVLGTTI M+ NI EVLMES
Sbjct: 262 YLHVLGDSIQSVGVMIGGAIIWYKPEWKIIDLICTLAFSIIVLGTTIGMIRNILEVLMES 321

Query: 357 TPREIDVSRIEKGLCEMHEVVAIHELHIWAITVGKILLA-------DACTDIVL*NMVDY 199
           TPREID +R+EKGLCEM EVVAIHELHIWAITVGK LLA       DA  D+VL  ++DY
Sbjct: 322 TPREIDATRLEKGLCEMDEVVAIHELHIWAITVGKFLLACHVMIKPDADADMVLDKVIDY 381

Query: 198 YTSIH 184
               H
Sbjct: 382 IRREH 386



 Score = 26.6 bits (57), Expect(2) = 4e-56
 Identities = 11/14 (78%), Positives = 13/14 (92%)
 Frame = -3

Query: 188 YIRRECNISHVTMQ 147
           YIRRE NI+HVT+Q
Sbjct: 381 YIRREHNITHVTIQ 394