BLASTX nr result
ID: Coptis25_contig00021982
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00021982
(1887 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002273501.2| PREDICTED: sterol 3-beta-glucosyltransferase... 387 e-158
emb|CBI23867.3| unnamed protein product [Vitis vinifera] 361 e-148
ref|XP_002528612.1| conserved hypothetical protein [Ricinus comm... 359 e-145
ref|XP_003571635.1| PREDICTED: sterol 3-beta-glucosyltransferase... 332 e-131
ref|NP_001183260.1| uncharacterized protein LOC100501651 [Zea ma... 323 e-129
>ref|XP_002273501.2| PREDICTED: sterol 3-beta-glucosyltransferase-like [Vitis vinifera]
Length = 599
Score = 387 bits (995), Expect(4) = e-158
Identities = 188/284 (66%), Positives = 226/284 (79%), Gaps = 2/284 (0%)
Frame = -3
Query: 1063 YGFSKEVVECPGYWPSNVRVCGLWFVPMEWQFSCNKCRDLSIQFSERYLHPKDELCSTYM 884
YGFSKEVVECPGYWPSNVRVCG WF+PMEW+FSCNKC ++S S R ++ + E+C +
Sbjct: 315 YGFSKEVVECPGYWPSNVRVCGFWFLPMEWEFSCNKCGEISASISLRRVNAEVEMCPAHT 374
Query: 883 DLQCFLMTSTSDVPIFIGFSSVGSMGFLRNSRAFLLVLQTVIQITNYRFILFTAGYEPLD 704
LQ FL T P+FIG SSVGSMGFLRN R FL V+ TV+ ITNYRFILF+AGYEPLD
Sbjct: 375 MLQSFLKTPAPMPPVFIGLSSVGSMGFLRNPREFLQVILTVLDITNYRFILFSAGYEPLD 434
Query: 703 SAIQMLA-EALSCFDRKICSEDGV-LFWDRLFCFSGTIPYNWLFQRCAVAVHHGGSGSTA 530
+A++++A EA S +R+ SEDG+ LF RLFCFSGTI YNWLF RC+ A+HHGGSGSTA
Sbjct: 435 AAVKVIAAEASSSLERRQSSEDGIFLFGGRLFCFSGTISYNWLFPRCSAAIHHGGSGSTA 494
Query: 529 AALHTGLPQVICPFLLDQFYWAERMFWLGVAPEPLDKYLLFPDKDDDTDIWKAANVLTNA 350
AAL G+PQV+CPF+LDQFYWAERMFWLGVAPEPL + LFPDK+D T I +AA VL+ A
Sbjct: 495 AALKAGIPQVLCPFMLDQFYWAERMFWLGVAPEPLKRNHLFPDKNDGTSIREAAVVLSRA 554
Query: 349 LNSALSPEIKACASEIAERIVSEDGVGEAVNAMKEEMKCQS*AN 218
++ ALSP++KA ASEIAERI EDGV EAV +KEE+ C++ N
Sbjct: 555 IDYALSPKVKARASEIAERISLEDGVSEAVKILKEEITCKNSTN 598
Score = 116 bits (290), Expect(4) = e-158
Identities = 64/144 (44%), Positives = 71/144 (49%), Gaps = 1/144 (0%)
Frame = -2
Query: 1550 EGWSLAELFRVRCXXXXXXXXXXXXXXSFERQFKKDIPLSFNHLQLVPPHKVCWNDVIHW 1371
EGWSLAELF VRC SFE F+K++PL + +LQ P KVCW DV HW
Sbjct: 211 EGWSLAELFHVRCIVAAPYVVPYSAPSSFEHHFRKELPLLYEYLQEAPTDKVCWKDVTHW 270
Query: 1370 MWPLFTEDWGSWRS-ELNLSPCPFMVWAFDK*REAIL*SYLSA*KARKYQYVFNLKSIFL 1194
MWPLFTEDWGSWRS +LNLSP PF
Sbjct: 271 MWPLFTEDWGSWRSDDLNLSPWPF------------------------------------ 294
Query: 1193 SFQMQDPVTGLPTSHDWVQSPMLL 1122
DPVTGLP HD SP+LL
Sbjct: 295 ----TDPVTGLPMWHDRYPSPVLL 314
Score = 79.3 bits (194), Expect(4) = e-158
Identities = 38/57 (66%), Positives = 46/57 (80%)
Frame = -3
Query: 1876 KKARAVFMAFGTKGDVLPIAAIAAGFATDQLQYQVFLITHSSHQNLIPHLAAKSLLF 1706
K+ AVFMAFGTKGDV PIAAIA+ FA+DQ QY V LITHS+H+NL HLA K++ +
Sbjct: 89 KRVLAVFMAFGTKGDVHPIAAIASAFASDQKQYHVVLITHSAHKNLTAHLAGKNVAY 145
Score = 48.5 bits (114), Expect(4) = e-158
Identities = 22/41 (53%), Positives = 31/41 (75%)
Frame = -1
Query: 1674 YQDHSTSESVEISFSMQKKIITKEHRNECLDVMGRIFGDGP 1552
+++H T S+E SFS+QK+IIT+EHR EC V+ IFG+ P
Sbjct: 157 HENHDTEGSLE-SFSLQKRIITREHRQECFSVVETIFGEEP 196
>emb|CBI23867.3| unnamed protein product [Vitis vinifera]
Length = 506
Score = 361 bits (926), Expect(4) = e-148
Identities = 181/284 (63%), Positives = 214/284 (75%), Gaps = 2/284 (0%)
Frame = -3
Query: 1063 YGFSKEVVECPGYWPSNVRVCGLWFVPMEWQFSCNKCRDLSIQFSERYLHPKDELCSTYM 884
YGFSKEVVECPGYWPSNVRVCG WF+PMEW+FS E+C +
Sbjct: 220 YGFSKEVVECPGYWPSNVRVCGFWFLPMEWEFSF-------------------EMCPAHT 260
Query: 883 DLQCFLMTSTSDVPIFIGFSSVGSMGFLRNSRAFLLVLQTVIQITNYRFILFTAGYEPLD 704
LQ FL T P+FIG SSVGSMGFLRN R FL V+ TV+ ITNYRFILF+AGYEPLD
Sbjct: 261 MLQSFLKTPAPMPPVFIGLSSVGSMGFLRNPREFLQVILTVLDITNYRFILFSAGYEPLD 320
Query: 703 SAIQMLA-EALSCFDRKICSEDGV-LFWDRLFCFSGTIPYNWLFQRCAVAVHHGGSGSTA 530
+A++++A EA S +R+ SEDG+ LF RLFCFSGTI YNWLF RC+ A+HHGGSGSTA
Sbjct: 321 AAVKVIAAEASSSLERRQSSEDGIFLFGGRLFCFSGTISYNWLFPRCSAAIHHGGSGSTA 380
Query: 529 AALHTGLPQVICPFLLDQFYWAERMFWLGVAPEPLDKYLLFPDKDDDTDIWKAANVLTNA 350
AAL G+PQV+CPF+LDQFYWAERMFWLGVAPEPL + LFPDK+D T I +AA VL+ A
Sbjct: 381 AALKAGIPQVLCPFMLDQFYWAERMFWLGVAPEPLKRNHLFPDKNDGTSIREAAVVLSRA 440
Query: 349 LNSALSPEIKACASEIAERIVSEDGVGEAVNAMKEEMKCQS*AN 218
++ ALSP++KA ASEIAERI EDGV EAV +KEE+ C++ N
Sbjct: 441 IDYALSPKVKARASEIAERISLEDGVSEAVKILKEEITCKNSTN 484
Score = 116 bits (290), Expect(4) = e-148
Identities = 64/144 (44%), Positives = 71/144 (49%), Gaps = 1/144 (0%)
Frame = -2
Query: 1550 EGWSLAELFRVRCXXXXXXXXXXXXXXSFERQFKKDIPLSFNHLQLVPPHKVCWNDVIHW 1371
EGWSLAELF VRC SFE F+K++PL + +LQ P KVCW DV HW
Sbjct: 116 EGWSLAELFHVRCIVAAPYVVPYSAPSSFEHHFRKELPLLYEYLQEAPTDKVCWKDVTHW 175
Query: 1370 MWPLFTEDWGSWRS-ELNLSPCPFMVWAFDK*REAIL*SYLSA*KARKYQYVFNLKSIFL 1194
MWPLFTEDWGSWRS +LNLSP PF
Sbjct: 176 MWPLFTEDWGSWRSDDLNLSPWPF------------------------------------ 199
Query: 1193 SFQMQDPVTGLPTSHDWVQSPMLL 1122
DPVTGLP HD SP+LL
Sbjct: 200 ----TDPVTGLPMWHDRYPSPVLL 219
Score = 72.0 bits (175), Expect(4) = e-148
Identities = 34/50 (68%), Positives = 41/50 (82%)
Frame = -3
Query: 1855 MAFGTKGDVLPIAAIAAGFATDQLQYQVFLITHSSHQNLIPHLAAKSLLF 1706
MAFGTKGDV PIAAIA+ FA+DQ QY V LITHS+H+NL HLA K++ +
Sbjct: 1 MAFGTKGDVHPIAAIASAFASDQKQYHVVLITHSAHKNLTAHLAGKNVAY 50
Score = 48.5 bits (114), Expect(4) = e-148
Identities = 22/41 (53%), Positives = 31/41 (75%)
Frame = -1
Query: 1674 YQDHSTSESVEISFSMQKKIITKEHRNECLDVMGRIFGDGP 1552
+++H T S+E SFS+QK+IIT+EHR EC V+ IFG+ P
Sbjct: 62 HENHDTEGSLE-SFSLQKRIITREHRQECFSVVETIFGEEP 101
>ref|XP_002528612.1| conserved hypothetical protein [Ricinus communis]
gi|223531957|gb|EEF33770.1| conserved hypothetical
protein [Ricinus communis]
Length = 512
Score = 359 bits (921), Expect(4) = e-145
Identities = 171/278 (61%), Positives = 212/278 (76%), Gaps = 1/278 (0%)
Frame = -3
Query: 1063 YGFSKEVVECPGYWPSNVRVCGLWFVPMEWQFSCNKCRDLSIQFSERYLHPKDELCSTYM 884
YGFSKE+VECP YWPSNVRVCG WF+P EWQFSC KC D+S S + E+C+ +M
Sbjct: 235 YGFSKEIVECPDYWPSNVRVCGFWFLPCEWQFSCKKCGDISAISSSESPRSETEMCADHM 294
Query: 883 DLQCFLMTSTSDVPIFIGFSSVGSMGFLRNSRAFLLVLQTVIQITNYRFILFTAGYEPLD 704
LQ FL S PIFIG SSVGSMGFL++ +AFL V+QTV++ITN++FILFT+GY+PLD
Sbjct: 295 KLQHFLKASVP--PIFIGLSSVGSMGFLKHPQAFLWVIQTVLEITNFKFILFTSGYDPLD 352
Query: 703 SAIQMLAEALSCFDRKICSEDGV-LFWDRLFCFSGTIPYNWLFQRCAVAVHHGGSGSTAA 527
A+++L SC D++ S +G+ LF RLFCF G IPYNWLF RC A+HHGGSGSTAA
Sbjct: 353 EAVRILNSETSCIDQRQYSNEGMCLFNGRLFCFPGMIPYNWLFPRCLAAIHHGGSGSTAA 412
Query: 526 ALHTGLPQVICPFLLDQFYWAERMFWLGVAPEPLDKYLLFPDKDDDTDIWKAANVLTNAL 347
AL+ G+PQVICPF+LDQFYWAERM+WLGVAPEP+ + L PDK ++ I AAN+L+ ++
Sbjct: 413 ALYAGIPQVICPFMLDQFYWAERMYWLGVAPEPVKQSYLVPDKINEMSIRVAANMLSKSI 472
Query: 346 NSALSPEIKACASEIAERIVSEDGVGEAVNAMKEEMKC 233
N LSP++KA A EIA RI EDGV EAV +K+EM C
Sbjct: 473 NDTLSPKVKARAIEIAARISLEDGVMEAVKILKQEMNC 510
Score = 119 bits (297), Expect(4) = e-145
Identities = 62/144 (43%), Positives = 74/144 (51%), Gaps = 1/144 (0%)
Frame = -2
Query: 1550 EGWSLAELFRVRCXXXXXXXXXXXXXXSFERQFKKDIPLSFNHLQLVPPHKVCWNDVIHW 1371
EGWSLAELF VRC SFE F+K++PL + +LQ P +KVCW DV HW
Sbjct: 131 EGWSLAELFHVRCVVAAPYVIPYSAPSSFEVHFRKELPLLYEYLQAAPINKVCWKDVTHW 190
Query: 1370 MWPLFTEDWGSWRS-ELNLSPCPFMVWAFDK*REAIL*SYLSA*KARKYQYVFNLKSIFL 1194
MWPLFTE+WGSWR+ +LNLS CP
Sbjct: 191 MWPLFTENWGSWRNDDLNLSSCP------------------------------------- 213
Query: 1193 SFQMQDPVTGLPTSHDWVQSPMLL 1122
+ DPVTGLPT HDW +P+LL
Sbjct: 214 ---LTDPVTGLPTWHDWPPAPLLL 234
Score = 69.7 bits (169), Expect(4) = e-145
Identities = 33/56 (58%), Positives = 42/56 (75%)
Frame = -3
Query: 1885 ESSKKARAVFMAFGTKGDVLPIAAIAAGFATDQLQYQVFLITHSSHQNLIPHLAAK 1718
E +K A+FMAFGTKGDV PIAAIAA FA DQ ++ V L+THS+H++L HL +
Sbjct: 6 EKKRKPVALFMAFGTKGDVYPIAAIAAAFACDQKRHNVVLVTHSAHESLRSHLEGR 61
Score = 40.0 bits (92), Expect(4) = e-145
Identities = 17/40 (42%), Positives = 26/40 (65%)
Frame = -1
Query: 1674 YQDHSTSESVEISFSMQKKIITKEHRNECLDVMGRIFGDG 1555
+ +H ++ S ++ S+ KKIIT+EHR EC + IFG G
Sbjct: 77 HNNHESTGSQGLALSLDKKIITREHRQECHSAVEGIFGHG 116
>ref|XP_003571635.1| PREDICTED: sterol 3-beta-glucosyltransferase-like [Brachypodium
distachyon]
Length = 522
Score = 332 bits (852), Expect(4) = e-131
Identities = 168/292 (57%), Positives = 209/292 (71%), Gaps = 14/292 (4%)
Frame = -3
Query: 1063 YGFSKEVVECPGYWPSNVRVCGLWFVPMEWQFSCNKCRDLSIQFSERYLHPKDE--LCST 890
YGFSKE+VE PGYWPS+ VCG WF+PM WQFSC+ CR L ++P E LC
Sbjct: 242 YGFSKEIVERPGYWPSSAHVCGFWFLPMAWQFSCDNCRGLLCG----NVNPSSEGILCGN 297
Query: 889 YMDLQCFLMTST-SDVPIFIGFSSVGSMGFLRNSRAFLLVLQTVIQITNYRFILFTAGYE 713
+ L+ +LM S+ S +PIFIG SS+GSMGFLRN +AFL+V++ VI+ T+YRFILF++GY+
Sbjct: 298 HSGLEHYLMGSSYSSLPIFIGLSSIGSMGFLRNPKAFLMVIKAVIESTDYRFILFSSGYQ 357
Query: 712 PLDSAIQMLAE-----------ALSCFDRKICSEDGVLFWDRLFCFSGTIPYNWLFQRCA 566
PLDSAI+ +A ALSC + +LF RLFCFSG+IPY+WLF +CA
Sbjct: 358 PLDSAIRFVASSVPESSELEATALSC-------DSTLLFNSRLFCFSGSIPYSWLFPKCA 410
Query: 565 VAVHHGGSGSTAAALHTGLPQVICPFLLDQFYWAERMFWLGVAPEPLDKYLLFPDKDDDT 386
A+HH GSGSTAAAL G+PQV CPFLLDQFYWAER+ WLGVAPEPL + L PD DD
Sbjct: 411 AAIHHAGSGSTAAALFAGIPQVACPFLLDQFYWAERLHWLGVAPEPLKRQHLVPDVDDTL 470
Query: 385 DIWKAANVLTNALNSALSPEIKACASEIAERIVSEDGVGEAVNAMKEEMKCQ 230
I AA+VL A+ SALSPEIKA A+ IA R+ EDGVGEA+ +KE++ C+
Sbjct: 471 SINNAADVLLGAIRSALSPEIKAQATRIAHRLAPEDGVGEALRTLKEKVLCE 522
Score = 98.2 bits (243), Expect(4) = e-131
Identities = 54/144 (37%), Positives = 66/144 (45%), Gaps = 1/144 (0%)
Frame = -2
Query: 1550 EGWSLAELFRVRCXXXXXXXXXXXXXXSFERQFKKDIPLSFNHLQLVPPHKVCWNDVIHW 1371
EGW LAELF+V+C SFERQFK+ PL + + Q P + VCW D+ HW
Sbjct: 138 EGWHLAELFQVKCIIAAPYFVPYSAPTSFERQFKQSFPLLYKYFQEAPTNTVCWTDIAHW 197
Query: 1370 MWPLFTEDWGSWRSE-LNLSPCPFMVWAFDK*REAIL*SYLSA*KARKYQYVFNLKSIFL 1194
MW LF E WGSWR++ LNLSP P+
Sbjct: 198 MWALFMETWGSWRNDCLNLSPIPY------------------------------------ 221
Query: 1193 SFQMQDPVTGLPTSHDWVQSPMLL 1122
DPVT LP H +SP+LL
Sbjct: 222 ----TDPVTNLPLWHVRAESPLLL 241
Score = 76.6 bits (187), Expect(4) = e-131
Identities = 36/57 (63%), Positives = 44/57 (77%)
Frame = -3
Query: 1876 KKARAVFMAFGTKGDVLPIAAIAAGFATDQLQYQVFLITHSSHQNLIPHLAAKSLLF 1706
++ RAVFMAFGT GDV PIAA+AA F+ DQ QY V ITHS+HQNL HLAA ++ +
Sbjct: 9 RRPRAVFMAFGTHGDVFPIAALAAAFSLDQQQYSVVFITHSAHQNLSAHLAASNVRY 65
Score = 33.5 bits (75), Expect(4) = e-131
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -1
Query: 1638 SFSMQKKIITKEHRNECLDVMGRIFGDGP 1552
SFS +K+ I EHR ECL + +FG+ P
Sbjct: 95 SFSRRKETIQIEHREECLSYVEEVFGNDP 123
>ref|NP_001183260.1| uncharacterized protein LOC100501651 [Zea mays]
gi|238010378|gb|ACR36224.1| unknown [Zea mays]
gi|413936800|gb|AFW71351.1| putative acetate/butyrate
kinase domain protein [Zea mays]
Length = 532
Score = 323 bits (828), Expect(4) = e-129
Identities = 156/284 (54%), Positives = 201/284 (70%), Gaps = 5/284 (1%)
Frame = -3
Query: 1063 YGFSKEVVECPGYWPSNVRVCGLWFVPMEWQFSCNKCRDLSIQFSERYLHPKDELCSTYM 884
YGFSKE+VECPGYWP N CG WF+PM+WQFSC+KC +LS + + LC+ +
Sbjct: 242 YGFSKEIVECPGYWPFNAHACGFWFLPMDWQFSCDKCMELSGNINSSF---GGMLCANHS 298
Query: 883 DLQCFLMTST-SDVPIFIGFSSVGSMGFLRNSRAFLLVLQTVIQITNYRFILFTAGYEPL 707
L+ FL + S PIF+G SS+G MGFLRN +AFL+VL+ I+ T+Y FILF++GY+PL
Sbjct: 299 SLEHFLTRNAYSSRPIFVGLSSIGRMGFLRNPKAFLMVLKAAIESTDYMFILFSSGYKPL 358
Query: 706 DSAIQMLA----EALSCFDRKICSEDGVLFWDRLFCFSGTIPYNWLFQRCAVAVHHGGSG 539
DSAIQ +A E+ + + +LF RLFCF G+IPY+WLF RCA A+HH GSG
Sbjct: 359 DSAIQSIASSVNESRGVDSPSLGGDSALLFNGRLFCFLGSIPYSWLFPRCAAAIHHAGSG 418
Query: 538 STAAALHTGLPQVICPFLLDQFYWAERMFWLGVAPEPLDKYLLFPDKDDDTDIWKAANVL 359
STAAAL G+PQV CPF++DQFYW+ER+ WLGVAPEPL + L PD DD I AA+VL
Sbjct: 419 STAAALLAGIPQVTCPFMMDQFYWSERLQWLGVAPEPLQRQNLVPDNDDALSIHNAADVL 478
Query: 358 TNALNSALSPEIKACASEIAERIVSEDGVGEAVNAMKEEMKCQS 227
A+ SALSPE KA A+ IA+R+ SEDG+GEA+ +K + Q+
Sbjct: 479 VGAIRSALSPETKAQAARIADRLSSEDGIGEALRILKARVLTQN 522
Score = 99.0 bits (245), Expect(4) = e-129
Identities = 55/144 (38%), Positives = 67/144 (46%), Gaps = 1/144 (0%)
Frame = -2
Query: 1550 EGWSLAELFRVRCXXXXXXXXXXXXXXSFERQFKKDIPLSFNHLQLVPPHKVCWNDVIHW 1371
EGW LAELF+V+C SF+RQFK+ PL + + Q P + VCW D+IHW
Sbjct: 138 EGWHLAELFQVKCVIAAPYFVPYSAPTSFKRQFKQTFPLLYKYFQEAPANTVCWTDIIHW 197
Query: 1370 MWPLFTEDWGSWRSE-LNLSPCPFMVWAFDK*REAIL*SYLSA*KARKYQYVFNLKSIFL 1194
MW LF E WGSWR++ LNLSP PF
Sbjct: 198 MWALFMESWGSWRNDCLNLSPIPFT----------------------------------- 222
Query: 1193 SFQMQDPVTGLPTSHDWVQSPMLL 1122
DPVT LP H +SP+LL
Sbjct: 223 -----DPVTNLPLWHVREESPLLL 241
Score = 75.1 bits (183), Expect(4) = e-129
Identities = 35/59 (59%), Positives = 46/59 (77%)
Frame = -3
Query: 1882 SSKKARAVFMAFGTKGDVLPIAAIAAGFATDQLQYQVFLITHSSHQNLIPHLAAKSLLF 1706
+ ++ RAVFMAFGT+GDV PIAA+AA FA DQ +Y V ITHS+H++L HLAA S+ +
Sbjct: 7 AGRRPRAVFMAFGTQGDVFPIAALAAAFACDQQEYAVVFITHSAHRSLSAHLAASSVRY 65
Score = 38.1 bits (87), Expect(4) = e-129
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = -1
Query: 1638 SFSMQKKIITKEHRNECLDVMGRIFGDGP 1552
SFSM+KK I EHR ECL + +FG+ P
Sbjct: 95 SFSMRKKAIQAEHRKECLSFVEEVFGNDP 123