BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00021842
         (1037 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002275928.1| PREDICTED: protein argonaute 4 [Vitis vinife...   337   3e-90
gb|ABC61505.1| AGO4-2, partial [Nicotiana benthamiana]                335   8e-90
gb|AFV15381.1| AGO4A [Solanum lycopersicum]                           335   8e-90
gb|ACZ04920.1| argonaute 4-like protein [Pelargonium x hortorum]      333   4e-89
ref|XP_004149653.1| PREDICTED: protein argonaute 4-like [Cucumis...   331   2e-88

>ref|XP_002275928.1| PREDICTED: protein argonaute 4 [Vitis vinifera]
            gi|296083994|emb|CBI24382.3| unnamed protein product
            [Vitis vinifera]
          Length = 913

 Score =  337 bits (864), Expect = 3e-90
 Identities = 186/318 (58%), Positives = 223/318 (70%), Gaps = 25/318 (7%)
 Frame = +2

Query: 158  MLCPSGVSSDSVLIQIKGRVLSPPQ---------------------KLVLPRKIEHWAIV 274
            ML   G+S    L QI+GRVL+ P+                     KLV P KIE WA+V
Sbjct: 442  MLRSCGISISRDLTQIEGRVLAAPRLKVGNGEDFFPRNGRWNFNNKKLVEPTKIERWAVV 501

Query: 275  NFSVSCDVQELSKDLIRYGKQHGILFKDPYVVIKENPSVRTESPLDRVENMLEQIRGKRP 454
            NFS  CD++ L ++LI+ G   GI    P+ V +ENP  R   P+ RVE M E+I+ K P
Sbjct: 502  NFSARCDIRNLVRELIKCGGMKGIHIDPPFDVFEENPQSRRAPPIVRVEKMFEEIQSKLP 561

Query: 455  FTPQFLLCLLPE-SDSAIYGPWKRKTRSEFGIVTQCMAPQHFYVDLGHLTNVLLNINMKL 631
              PQFLLCLLPE  +S +YGPWKRK  SE+GIVTQC+AP    V+  +LTNVLL IN KL
Sbjct: 562  GAPQFLLCLLPERKNSDLYGPWKRKNLSEYGIVTQCIAPTR--VNDQYLTNVLLKINAKL 619

Query: 632  GGSKKFIDLEHS--IPLISKASTLFLGMSVSHRSP-ESDVPSIAAVVSSRQWPLISCYRA 802
            GG    + +EHS  IP++SK  T+ LGM VSH SP +SDVPSIAAVVSSRQWPLIS YRA
Sbjct: 620  GGLNSMLAVEHSPSIPIVSKGPTIILGMDVSHGSPGQSDVPSIAAVVSSRQWPLISRYRA 679

Query: 803  SMRTQSPKVEIIDSLFKQVSDNIDEGIVRELLLDFSANSGGRMPEQIVIFRDGVSESHFH 982
            S+RTQSPKVE+IDSL+K+VS+  DEGI+RELLLDF  +SG R P+QI+IFRDGVSES F+
Sbjct: 680  SVRTQSPKVEMIDSLYKRVSETEDEGIIRELLLDFYVSSGKRKPDQIIIFRDGVSESQFN 739

Query: 983  QVLNFELDQIKEACKFLD 1036
            QVLN ELDQI EACKFLD
Sbjct: 740  QVLNIELDQIIEACKFLD 757


>gb|ABC61505.1| AGO4-2, partial [Nicotiana benthamiana]
          Length = 905

 Score =  335 bits (860), Expect = 8e-90
 Identities = 182/317 (57%), Positives = 222/317 (70%), Gaps = 24/317 (7%)
 Frame = +2

Query: 158  MLCPSGVSSDSVLIQIKGRVLSPPQ--------------------KLVLPRKIEHWAIVN 277
            +L   G+S  S   Q++GRVLSPP+                    +LV P KIE WA+VN
Sbjct: 435  LLRACGISISSNFTQVEGRVLSPPKLKTGGDDFVPRNGRWNFNNKRLVDPTKIERWAVVN 494

Query: 278  FSVSCDVQELSKDLIRYGKQHGILFKDPYVVIKENPSVRTESPLDRVENMLEQIRGKRPF 457
            FS  C++Q L  DLI+ GK  GI+ +DP+ V +E+P  R   PL RVE M E+++ K P 
Sbjct: 495  FSARCNIQGLISDLIKCGKMKGIMVEDPFDVFEESPQFRRAPPLVRVEKMFEEVQSKLPG 554

Query: 458  TPQFLLCLLPESDSA-IYGPWKRKTRSEFGIVTQCMAPQHFYVDLGHLTNVLLNINMKLG 634
             P+FLLCLLPE  +  IYGPWKRK  +EFGIVTQC+AP    V+  ++TNVLL IN KLG
Sbjct: 555  APKFLLCLLPERKNCDIYGPWKRKNLAEFGIVTQCIAPTR--VNDQYITNVLLKINAKLG 612

Query: 635  GSKKFIDLEHS--IPLISKASTLFLGMSVSHRSP-ESDVPSIAAVVSSRQWPLISCYRAS 805
            G    + +EH+  IP++SK  T+ LGM VSH SP +SDVPSIAAVVSSRQWP IS YRAS
Sbjct: 613  GLNSMLTVEHAPAIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRAS 672

Query: 806  MRTQSPKVEIIDSLFKQVSDNIDEGIVRELLLDFSANSGGRMPEQIVIFRDGVSESHFHQ 985
            +RTQSPKVE+ID+LFK+ SD  DEGI+RE LLDF  +SG R PE I+IFRDGVSES F+Q
Sbjct: 673  VRTQSPKVEMIDNLFKRASDTEDEGIMREALLDFYVSSGKRKPEHIIIFRDGVSESQFNQ 732

Query: 986  VLNFELDQIKEACKFLD 1036
            VLN ELDQI EACKFLD
Sbjct: 733  VLNIELDQIIEACKFLD 749


>gb|AFV15381.1| AGO4A [Solanum lycopersicum]
          Length = 909

 Score =  335 bits (860), Expect = 8e-90
 Identities = 183/317 (57%), Positives = 222/317 (70%), Gaps = 24/317 (7%)
 Frame = +2

Query: 158  MLCPSGVSSDSVLIQIKGRVLSPPQ--------------------KLVLPRKIEHWAIVN 277
            +L   G+S  +   QI+GRVL PP+                    +LV P KIE WA+VN
Sbjct: 439  LLRSCGISISNNFTQIEGRVLPPPKLKTGGDDFVPRNGRWNFNNKRLVDPTKIERWAVVN 498

Query: 278  FSVSCDVQELSKDLIRYGKQHGILFKDPYVVIKENPSVRTESPLDRVENMLEQIRGKRPF 457
            FS  C+VQ L  DLI+ GKQ GI+ +DP+ V +E+P VR   PL RVE M EQ++ K P 
Sbjct: 499  FSARCNVQGLVSDLIKCGKQKGIMVEDPFDVFEESPQVRRAPPLVRVEKMFEQVQSKLPG 558

Query: 458  TPQFLLCLLPESDSA-IYGPWKRKTRSEFGIVTQCMAPQHFYVDLGHLTNVLLNINMKLG 634
             P+FLLCLLPE  +  +YGPWKRK  +E+GIVTQC+AP    V+  ++TNVLL IN KLG
Sbjct: 559  APKFLLCLLPERKNCDVYGPWKRKNLAEYGIVTQCIAPTR--VNDQYITNVLLKINAKLG 616

Query: 635  GSKKFIDLEHS--IPLISKASTLFLGMSVSHRSP-ESDVPSIAAVVSSRQWPLISCYRAS 805
            G    + +EHS  IP++SK  T+ LGM VSH SP +SDVPSIAAVVSSRQWP IS YRAS
Sbjct: 617  GLNSMLTVEHSPAIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRAS 676

Query: 806  MRTQSPKVEIIDSLFKQVSDNIDEGIVRELLLDFSANSGGRMPEQIVIFRDGVSESHFHQ 985
            +RTQSPKVE+ID+LFK+ SD  D+GI+RE LLDF  +SG R PE I+IFRDGVSES F Q
Sbjct: 677  VRTQSPKVEMIDNLFKRTSDTEDDGIMREALLDFYVSSGKRKPEHIIIFRDGVSESQFSQ 736

Query: 986  VLNFELDQIKEACKFLD 1036
            VLN ELDQI EACKFLD
Sbjct: 737  VLNVELDQIIEACKFLD 753


>gb|ACZ04920.1| argonaute 4-like protein [Pelargonium x hortorum]
          Length = 933

 Score =  333 bits (854), Expect = 4e-89
 Identities = 184/319 (57%), Positives = 222/319 (69%), Gaps = 25/319 (7%)
 Frame = +2

Query: 155  RMLCPSGVSSDSVLIQIKGRVLSPPQ---------------------KLVLPRKIEHWAI 271
            RML  SG+S  S   Q++GRVL  P+                     KLV P KIE WA+
Sbjct: 460  RMLRSSGISISSNFTQVEGRVLQAPKLKVGNGEDFFPRNGRWNFNNKKLVDPTKIEKWAV 519

Query: 272  VNFSVSCDVQELSKDLIRYGKQHGILFKDPYVVIKENPSVRTESPLDRVENMLEQIRGKR 451
            VNFS  CD++ L +DLI+ G+  GI  + P+ V +E+P  R   P+ RVE M E I+ K 
Sbjct: 520  VNFSARCDIRGLVRDLIKCGEMKGIRVEAPFDVFEESPQCRRAPPMVRVEKMFEDIQSKL 579

Query: 452  PFTPQFLLCLLPE-SDSAIYGPWKRKTRSEFGIVTQCMAPQHFYVDLGHLTNVLLNINMK 628
            P  PQFLLCLLPE  +S +YGPWKRK  SE+GIVTQC+AP    V+  +LTNVLL IN K
Sbjct: 580  PGAPQFLLCLLPERKNSELYGPWKRKNLSEYGIVTQCIAPTR--VNDQYLTNVLLKINAK 637

Query: 629  LGGSKKFIDLEHS--IPLISKASTLFLGMSVSHRSP-ESDVPSIAAVVSSRQWPLISCYR 799
            LGG    + +EHS  IP++SK  T+ +GM VSH SP +SDVPSIAAVVSSRQWP IS YR
Sbjct: 638  LGGLNSMLAIEHSPSIPMVSKVPTIIVGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYR 697

Query: 800  ASMRTQSPKVEIIDSLFKQVSDNIDEGIVRELLLDFSANSGGRMPEQIVIFRDGVSESHF 979
            AS+RTQSPKVE+IDSLFK+ SD  D+GI+RELLLDF  +S  R P+QI+IFRDGVSES F
Sbjct: 698  ASVRTQSPKVEMIDSLFKKTSDTEDDGIMRELLLDFYVSSQKRKPDQIIIFRDGVSESQF 757

Query: 980  HQVLNFELDQIKEACKFLD 1036
            +QVLN ELDQI EACKFLD
Sbjct: 758  NQVLNIELDQIIEACKFLD 776


>ref|XP_004149653.1| PREDICTED: protein argonaute 4-like [Cucumis sativus]
          Length = 915

 Score =  331 bits (849), Expect = 2e-88
 Identities = 178/318 (55%), Positives = 223/318 (70%), Gaps = 25/318 (7%)
 Frame = +2

Query: 158  MLCPSGVSSDSVLIQIKGRVLSPPQ---------------------KLVLPRKIEHWAIV 274
            ML   G++ +S  IQ++GRVL  P+                     KL  P KIE WA+V
Sbjct: 444  MLRSCGIAINSSFIQVEGRVLPAPKLKVGNGEDFFPRNGRWNFNNKKLAQPTKIERWAVV 503

Query: 275  NFSVSCDVQELSKDLIRYGKQHGILFKDPYVVIKENPSVRTESPLDRVENMLEQIRGKRP 454
            NFS  CD + L +DLI+ G   GI  + P+ V +ENP  R   P+ RVE M E+++ K P
Sbjct: 504  NFSARCDTRGLVRDLIKCGDMKGIAIEAPFDVFEENPQFRRAPPMVRVEKMFEEVQSKLP 563

Query: 455  FTPQFLLCLLPE-SDSAIYGPWKRKTRSEFGIVTQCMAPQHFYVDLGHLTNVLLNINMKL 631
              PQFLLCLLPE  +S +YGPWK+K  +EFGIVTQC+AP    V+  +LTNVLL IN KL
Sbjct: 564  GQPQFLLCLLPERKNSDLYGPWKKKNLAEFGIVTQCIAPTR--VNDQYLTNVLLKINAKL 621

Query: 632  GGSKKFIDLEHS--IPLISKASTLFLGMSVSHRSP-ESDVPSIAAVVSSRQWPLISCYRA 802
            GG    + +EHS  IP++SK  T+ LGM VSH SP +SD+PSIAAVVSSRQWPLIS YRA
Sbjct: 622  GGLNSLLAVEHSPSIPMVSKVPTIILGMDVSHGSPGQSDIPSIAAVVSSRQWPLISRYRA 681

Query: 803  SMRTQSPKVEIIDSLFKQVSDNIDEGIVRELLLDFSANSGGRMPEQIVIFRDGVSESHFH 982
            ++RTQSPKVE+IDSL+K++SD  D+GI+RELLLDF  +SG R P+QI+IFRDGVSES F+
Sbjct: 682  AVRTQSPKVEMIDSLYKRISDTEDDGIMRELLLDFYTSSGKRKPDQIIIFRDGVSESQFN 741

Query: 983  QVLNFELDQIKEACKFLD 1036
            QVLN ELDQI ++CKFLD
Sbjct: 742  QVLNVELDQIIQSCKFLD 759