BLASTX nr result
ID: Coptis25_contig00021781
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00021781
(1842 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABC69377.1| CYP81B2v2 [Nicotiana tabacum] 468 e-129
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V... 464 e-128
emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera] 463 e-128
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum] 462 e-127
gb|ABC69378.1| CYP81B2v1 [Nicotiana tabacum] gi|158635852|dbj|BA... 462 e-127
>gb|ABC69377.1| CYP81B2v2 [Nicotiana tabacum]
Length = 511
Score = 468 bits (1203), Expect = e-129
Identities = 230/458 (50%), Positives = 317/458 (69%), Gaps = 6/458 (1%)
Frame = +1
Query: 313 LSLKHGSIMFLKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQNYTALGWV 492
+S ++GS++ L+FG R VL VSSP+ EE T NDI+FANRP L+AG+HLG N+T+L W
Sbjct: 59 ISERYGSVLLLEFGSRKVLLVSSPSAAEECLTKNDIIFANRPLLMAGKHLGYNFTSLAWS 118
Query: 493 PYGEHWRNLRRVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLF 672
YG+HWRNLRR+TS+E+F +E+K VK + S + K +D++SM F
Sbjct: 119 SYGDHWRNLRRITSVEMFSTHRLQMLHGIRIDEVKSMVKRLNSSAMAE--KSVDMKSMFF 176
Query: 673 DLTVNIMMRIVVGRPCYGENM-GIEEKSRMREFLKETFVPNMALNLGDIYPVFRRFFSFV 849
+L +N+MMR + G+ YGEN+ IEE +R + ++ETF A N+GD P +
Sbjct: 177 ELMLNVMMRTIAGKRYYGENVEDIEEATRFKGLVQETFRIGGATNIGDFLPALKLLVR-- 234
Query: 850 GLDKKLVKLQSTREEFLQGLIEETRIKR-----VDDSIDREKNRTTVTDGLLSLQKMEPD 1014
L+K L+ LQ R+EF+Q LI++ R + V DS + E N+ + + LL+LQ+ EP+
Sbjct: 235 KLEKSLIVLQENRDEFMQELIKDCRKRMEKEGTVTDS-EIEGNKKCLIEVLLTLQENEPE 293
Query: 1015 YYSDDAIQGIISIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGRLIDES 1194
YY D+ I+ ++ ++ +AGTDTS TMEWA+SL+LNHPE L+KA+AEID HI + RL+DES
Sbjct: 294 YYKDEIIRSLMLVLLSAGTDTSVGTMEWALSLMLNHPETLKKAQAEIDEHIGHERLVDES 353
Query: 1195 DLSNLPYLHCILNETLRLFPAGPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKL 1374
D++NLPYL CI+NET R++PAGPLLVPH SSEE T+GGY++P GTMLLVN +AIH DPKL
Sbjct: 354 DINNLPYLRCIINETFRMYPAGPLLVPHESSEETTVGGYRVPGGTMLLVNLWAIHNDPKL 413
Query: 1375 WEEPNSFKPERFTGVQGEREGFKFIPFGLGRRACPXXXXXXXXXXXXXXTLIQCFEWKRL 1554
W+EP FKPERF G+ G R+G+K +PFG GRR+CP +IQCF+W+R+
Sbjct: 414 WDEPRKFKPERFQGLDGVRDGYKMMPFGSGRRSCPGEGLAVRMVALSLGCIIQCFDWQRI 473
Query: 1555 GPELVDLTEWPGTTMPKAESLEATYRPRPQLMQFLSQL 1668
G ELVD+TE G T+PKA+ L A PRP++ LSQ+
Sbjct: 474 GEELVDMTEGTGLTLPKAQPLVAKCSPRPKMANLLSQI 511
>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
Length = 503
Score = 464 bits (1194), Expect = e-128
Identities = 233/453 (51%), Positives = 311/453 (68%), Gaps = 1/453 (0%)
Frame = +1
Query: 313 LSLKHGSIMFLKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQNYTALGWV 492
+S ++G I+F++FG R V+ VSSP+ EE FT NDIVFANRP L+AG+HLG NYT L W
Sbjct: 56 ISNQYGPILFIRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYTTLTWA 115
Query: 493 PYGEHWRNLRRVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLF 672
PYG+HWRNLRR+ S+EI +E++ + ++F + ++ +D++SM F
Sbjct: 116 PYGQHWRNLRRIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDMKSMFF 175
Query: 673 DLTVNIMMRIVVGRPCYGENMG-IEEKSRMREFLKETFVPNMALNLGDIYPVFRRFFSFV 849
+LT+N MMR++ G+ YG+N+ +EE + RE + ETF + A N+ D P F ++
Sbjct: 176 ELTLNNMMRMISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVDFVP-FSKWIGLN 234
Query: 850 GLDKKLVKLQSTREEFLQGLIEETRIKRVDDSIDREKNRTTVTDGLLSLQKMEPDYYSDD 1029
G++KKLV LQ R+ F+Q LIEE R R E T+ D LLSLQ+ EP+ Y+DD
Sbjct: 235 GIEKKLVILQGKRDGFMQNLIEEHRRMRSPC----EGRSKTMLDVLLSLQETEPECYTDD 290
Query: 1030 AIQGIISIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGRLIDESDLSNL 1209
I+G++ +M +AGTDTS+ TMEWAMSLLLN+PE LEKA+AEIDSH+ RLIDE D++ L
Sbjct: 291 IIRGMMQVMLSAGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSRLIDELDIAEL 350
Query: 1210 PYLHCILNETLRLFPAGPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPN 1389
PYL I+ ETLR++PA PLLVPH SSEECT+GG+++P GTMLLVN +AI DP LW EP+
Sbjct: 351 PYLRGIIKETLRMYPAAPLLVPHESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPS 410
Query: 1390 SFKPERFTGVQGEREGFKFIPFGLGRRACPXXXXXXXXXXXXXXTLIQCFEWKRLGPELV 1569
FKPERF G +G+R GF F PFG GRR CP +LIQ FEW+R+ E+V
Sbjct: 411 KFKPERFQGPEGQRNGFMFSPFGAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMV 470
Query: 1570 DLTEWPGTTMPKAESLEATYRPRPQLMQFLSQL 1668
D++E G TMPKA+SL A RPRP ++ LSQL
Sbjct: 471 DMSEGTGLTMPKAQSLVAKCRPRPSMVSLLSQL 503
>emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]
Length = 1001
Score = 463 bits (1192), Expect = e-128
Identities = 233/453 (51%), Positives = 311/453 (68%), Gaps = 1/453 (0%)
Frame = +1
Query: 313 LSLKHGSIMFLKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQNYTALGWV 492
+S ++G I+F++FG R V+ VSSP+ EE FT NDIVFANRP L+AG+HLG NYT L W
Sbjct: 554 ISNQYGPILFIRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYTTLTWA 613
Query: 493 PYGEHWRNLRRVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLF 672
PYG+HWRNLRR+ S+EI +E++ + ++F + ++ +D++SM F
Sbjct: 614 PYGQHWRNLRRIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDMKSMFF 673
Query: 673 DLTVNIMMRIVVGRPCYGENMG-IEEKSRMREFLKETFVPNMALNLGDIYPVFRRFFSFV 849
+LT+N MMR++ G+ YG+N+ +EE + RE + ETF + A N+ D P F ++
Sbjct: 674 ELTLNNMMRMISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVDFVP-FSKWIGLN 732
Query: 850 GLDKKLVKLQSTREEFLQGLIEETRIKRVDDSIDREKNRTTVTDGLLSLQKMEPDYYSDD 1029
G++KKLV LQ R+ F+Q LIEE R R E T+ D LLSLQ+ EP+ Y+DD
Sbjct: 733 GIEKKLVILQGKRDGFMQNLIEEHRRMRSPC----EXRSKTMLDVLLSLQETEPECYTDD 788
Query: 1030 AIQGIISIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGRLIDESDLSNL 1209
I+G++ +M +AGTDTS+ TMEWAMSLLLN+PE LEKA+AEIDSH+ RLIDE D++ L
Sbjct: 789 IIRGMMQVMLSAGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSRLIDELDIAXL 848
Query: 1210 PYLHCILNETLRLFPAGPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPN 1389
PYL I+ ETLR++PA PLLVPH SSEECT+GG+++P GTMLLVN +AI DP LW EP+
Sbjct: 849 PYLRGIIMETLRMYPAAPLLVPHESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPS 908
Query: 1390 SFKPERFTGVQGEREGFKFIPFGLGRRACPXXXXXXXXXXXXXXTLIQCFEWKRLGPELV 1569
FKPERF G +G+R GF F PFG GRR CP +LIQ FEW+R+ E+V
Sbjct: 909 KFKPERFQGPEGQRNGFMFSPFGAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMV 968
Query: 1570 DLTEWPGTTMPKAESLEATYRPRPQLMQFLSQL 1668
D++E G TMPKA+SL A RPRP ++ LSQL
Sbjct: 969 DMSEGTGLTMPKAQSLVAKCRPRPSMVSLLSQL 1001
>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
Length = 507
Score = 462 bits (1190), Expect = e-127
Identities = 228/456 (50%), Positives = 325/456 (71%), Gaps = 3/456 (0%)
Frame = +1
Query: 310 SLSLKHGSIMFLKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQNYTALGW 489
++S ++G +++ +FG R VL VSSP+ EE T NDI FANRP L+ G+H G NYT L W
Sbjct: 56 NISNQYGPVLYFQFGSRPVLVVSSPSAAEECLTKNDIGFANRPRLMIGKHSGNNYTTLAW 115
Query: 490 VPYGEHWRNLRRVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSML 669
YG++WRNLRR++++E+F +E+K V+ + + E+ ++++S+
Sbjct: 116 ASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLVAGY---EYHKVEMKSVF 172
Query: 670 FDLTVNIMMRIVVGRPCYGENMG-IEEKSRMREFLKETFVPNMALNLGDIYPVFRRFFSF 846
F+LT+NIMMR++ G+ YGE++ +E+ + +E ++ETF + A N+GD P+ R + +
Sbjct: 173 FELTLNIMMRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLR-WIGY 231
Query: 847 VGLDKKLVKLQSTREEFLQGLIEETRIKRVDD-SIDREKNRTTVTDGLLSLQKMEPDYYS 1023
G++K+L++L++ R+EFLQ LI+E R RV+ S E+ + T+ D LLSLQ+ EP+YY+
Sbjct: 232 KGIEKRLIRLKNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYT 291
Query: 1024 DDAIQGIISIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGRLIDESDLS 1203
D+ I+G+I I+F AGTDTS+ TMEWAMSLLLN+P++++KA++EID IE GR IDESDL+
Sbjct: 292 DEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLN 351
Query: 1204 NLPYLHCILNETLRLFPAGPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEE 1383
LPYLHCI+NETLR++PAGPLL+PH SSE+C IGGY IP GTMLLVN +AI DP LW+E
Sbjct: 352 KLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKE 411
Query: 1384 PNSFKPERFTGVQ-GEREGFKFIPFGLGRRACPXXXXXXXXXXXXXXTLIQCFEWKRLGP 1560
P FKPERF G Q G R+GFK +PFG GRR CP +L+QCF+W R+G
Sbjct: 412 PRKFKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGE 471
Query: 1561 ELVDLTEWPGTTMPKAESLEATYRPRPQLMQFLSQL 1668
E+V+++E G T+PK LEA RPR ++ FLSQ+
Sbjct: 472 EMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNFLSQV 507
>gb|ABC69378.1| CYP81B2v1 [Nicotiana tabacum] gi|158635852|dbj|BAF91366.1| cytochrome
P450 [Nicotiana tabacum]
Length = 511
Score = 462 bits (1190), Expect = e-127
Identities = 230/458 (50%), Positives = 315/458 (68%), Gaps = 6/458 (1%)
Frame = +1
Query: 313 LSLKHGSIMFLKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQNYTALGWV 492
+S ++GS++ L+FG R VL VSSP+ EE T NDI+FANRP L+AG+HLG N+T+L W
Sbjct: 59 ISERYGSVLLLEFGSRKVLLVSSPSAAEECLTKNDIIFANRPLLMAGKHLGYNFTSLAWS 118
Query: 493 PYGEHWRNLRRVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLF 672
YG+HWRNLRR+TS+E+F +E+K VK + S + K +D++SM F
Sbjct: 119 SYGDHWRNLRRITSVEMFSTHRLQMLHGIRIDEVKSMVKRLNSSAIAE--KSVDMKSMFF 176
Query: 673 DLTVNIMMRIVVGRPCYGENM-GIEEKSRMREFLKETFVPNMALNLGDIYPVFRRFFSFV 849
+L +N+MMR + G+ YGEN+ IEE +R + ++ETF A N+GD P +
Sbjct: 177 ELMLNVMMRTIAGKRYYGENVEDIEEATRFKGLVQETFRIGGATNIGDFLPALKLLVR-- 234
Query: 850 GLDKKLVKLQSTREEFLQGLIEETRIKR-----VDDSIDREKNRTTVTDGLLSLQKMEPD 1014
L+K L+ LQ R+EF+Q LI++ R + V DS + E N+ + + LL+LQ+ EP+
Sbjct: 235 KLEKSLIVLQENRDEFMQELIKDCRKRMEKEGTVTDS-EIEGNKKCLIEVLLTLQENEPE 293
Query: 1015 YYSDDAIQGIISIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGRLIDES 1194
YY D+ I+ ++ ++ +AGTDTS TMEWA+SL+LNHPE L+KA+AEID HI + RL+DES
Sbjct: 294 YYKDEIIRSLMLVLLSAGTDTSVGTMEWALSLMLNHPETLKKAQAEIDEHIGHERLVDES 353
Query: 1195 DLSNLPYLHCILNETLRLFPAGPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKL 1374
D++NLPYL CI+NET R++PAGPLLVPH SSEE T+GGY++P GTMLLVN +AIH DPKL
Sbjct: 354 DINNLPYLRCIINETFRMYPAGPLLVPHESSEETTVGGYRVPGGTMLLVNLWAIHNDPKL 413
Query: 1375 WEEPNSFKPERFTGVQGEREGFKFIPFGLGRRACPXXXXXXXXXXXXXXTLIQCFEWKRL 1554
W+EP FKPERF G++G R+G+K +PFG GRR+CP +IQCF+W+RL
Sbjct: 414 WDEPRKFKPERFEGLEGVRDGYKMMPFGSGRRSCPGEGLAIRMVALSLGCIIQCFDWQRL 473
Query: 1555 GPELVDLTEWPGTTMPKAESLEATYRPRPQLMQFLSQL 1668
G LVD TE G T+PKA+ L A PRP + LSQ+
Sbjct: 474 GEGLVDKTEGTGLTLPKAQPLVAKCSPRPIMANLLSQI 511