BLASTX nr result
ID: Coptis25_contig00021617
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00021617
(1505 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis ... 673 0.0
emb|CBI17531.3| unnamed protein product [Vitis vinifera] 673 0.0
ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-de... 671 0.0
ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|2... 668 0.0
gb|AER08630.1| steroid 22-alpha hydroxylase [Populus euphratica] 667 0.0
>ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis vinifera]
Length = 487
Score = 673 bits (1736), Expect = 0.0
Identities = 326/438 (74%), Positives = 374/438 (85%), Gaps = 8/438 (1%)
Frame = -2
Query: 1291 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 1112
MS+L+F + +I L+I L KRK+ LNLPPGN GWPFLGETIGYLKPYSA + G+
Sbjct: 1 MSDLEFFLLLLASISVPLVILNLIKRKQSGLNLPPGNTGWPFLGETIGYLKPYSATSIGD 60
Query: 1111 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 932
FMEQHISRFG+IYKS+LFG TIVS D+ LNRFILQN+G+LFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKWSML 120
Query: 931 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 752
VLVGDMH++MRTISLNF+S RLRTHL+ +V H LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 751 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 572
MAKHIMS++PGKPE+E+L++EY+TFMKGVVS PLNFPGTAYRKAL+SRS+ILK IE M
Sbjct: 181 LMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIELKM 240
Query: 571 DEKIRKTNGHS-DKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYF 395
+E+I+K G + +EDDDLLGWVLKHSNL+TEQILDL+LS+LFAGHET+S++I+LAIYF
Sbjct: 241 EERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALAIYF 300
Query: 394 LQGCPEAVQELREEHLGITKAKKQAGESGLNWEDYKQMEFTQCVISETLRLGNVVRFLHR 215
L+GCP AVQ+LREEHL I++AKKQ+GE L+WEDYK+MEFTQCVISETLRLGNVVRFLHR
Sbjct: 301 LEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRFLHR 360
Query: 214 KALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQKG-------KTSAPT 56
KALKDVRYKGYDIPCGWKVLPV AAVHLDP FDQPQ FNPWRWQ S T
Sbjct: 361 KALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQNNGNRGNSTSWSTAT 420
Query: 55 SNNFMPFGGGPRLCAGSE 2
+ NFMPFGGGPRLCAGSE
Sbjct: 421 NQNFMPFGGGPRLCAGSE 438
>emb|CBI17531.3| unnamed protein product [Vitis vinifera]
Length = 488
Score = 673 bits (1736), Expect = 0.0
Identities = 326/439 (74%), Positives = 375/439 (85%), Gaps = 9/439 (2%)
Frame = -2
Query: 1291 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 1112
MS+L+F + +I L+I L KRK+ LNLPPGN GWPFLGETIGYLKPYSA + G+
Sbjct: 1 MSDLEFFLLLLASISVPLVILNLIKRKQSGLNLPPGNTGWPFLGETIGYLKPYSATSIGD 60
Query: 1111 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 932
FMEQHISRFG+IYKS+LFG TIVS D+ LNRFILQN+G+LFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKWSML 120
Query: 931 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 752
VLVGDMH++MRTISLNF+S RLRTHL+ +V H LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 751 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 572
MAKHIMS++PGKPE+E+L++EY+TFMKGVVS PLNFPGTAYRKAL+SRS+ILK IE M
Sbjct: 181 LMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIELKM 240
Query: 571 DEKIRKTNGHS-DKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYF 395
+E+I+K G + +EDDDLLGWVLKHSNL+TEQILDL+LS+LFAGHET+S++I+LAIYF
Sbjct: 241 EERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALAIYF 300
Query: 394 LQGCPEAVQELREEHLGITKAKKQAGESGLNWEDYKQMEFTQCVISETLRLGNVVRFLHR 215
L+GCP AVQ+LREEHL I++AKKQ+GE L+WEDYK+MEFTQCVISETLRLGNVVRFLHR
Sbjct: 301 LEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRFLHR 360
Query: 214 KALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQKG--------KTSAP 59
KALKDVRYKGYDIPCGWKVLPV AAVHLDP FDQPQ FNPWRWQ+ S
Sbjct: 361 KALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQQNNGNRGNSTSWSTA 420
Query: 58 TSNNFMPFGGGPRLCAGSE 2
T+ NFMPFGGGPRLCAGSE
Sbjct: 421 TNQNFMPFGGGPRLCAGSE 439
>ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-deoxocathasterone or
6-oxocampestanol to cathasterone [Populus trichocarpa]
gi|222853127|gb|EEE90674.1| cytochrome P450 probable
campestanol to 6-deoxocathasterone or 6-oxocampestanol to
cathasterone [Populus trichocarpa]
Length = 486
Score = 671 bits (1732), Expect = 0.0
Identities = 322/439 (73%), Positives = 376/439 (85%), Gaps = 9/439 (2%)
Frame = -2
Query: 1291 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 1112
MS + + + P+IL+LLL++ L +RK+ NLPPGNMGWPFLGETIGYLKPYSA + GE
Sbjct: 1 MSHSELVVFLLPSILSLLLLFILVQRKQVRFNLPPGNMGWPFLGETIGYLKPYSATSIGE 60
Query: 1111 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 932
FMEQHISR+GKIYKS+LFG TIVS DA LNRFILQN+GRLFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPKSIGGILGKWSML 120
Query: 931 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 752
VLVGDMH++MR ISLNF+S ARLRTHL+++VE LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRDMRIISLNFLSHARLRTHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 751 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 572
MAKHIMS++PGKPE+E+L++EY+TFMKGVVS P+N PGT YRKALKSRS ILK IE+ M
Sbjct: 181 LMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPINLPGTPYRKALKSRSIILKFIERKM 240
Query: 571 DEKIRKTNGHSDKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYFL 392
+E+I +T G + +EDDDLLGWVLKHSNL+TEQILDLILS+LFAGHET+S+SI+LAIYFL
Sbjct: 241 EERIAETKGGVENLEDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAIYFL 300
Query: 391 QGCPEAVQELREEHLGITKAKKQAGESGLNWEDYKQMEFTQCVISETLRLGNVVRFLHRK 212
Q CP A+Q+L+EEH+ I++AKK++GE+ LNW+DYK+MEFTQCVISETLRLGNVVRFLHRK
Sbjct: 301 QACPGAIQQLKEEHIQISRAKKRSGETELNWDDYKKMEFTQCVISETLRLGNVVRFLHRK 360
Query: 211 ALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQK---------GKTSAP 59
ALKDVRYKGYDIPCGWKVLPV +AVHLD FDQPQ FNPWRWQ+ +
Sbjct: 361 ALKDVRYKGYDIPCGWKVLPVISAVHLDSTLFDQPQHFNPWRWQQHNNARGASTSSSGTT 420
Query: 58 TSNNFMPFGGGPRLCAGSE 2
+SN FMPFGGGPRLCAGSE
Sbjct: 421 SSNYFMPFGGGPRLCAGSE 439
>ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|222836894|gb|EEE75287.1|
predicted protein [Populus trichocarpa]
Length = 488
Score = 668 bits (1723), Expect = 0.0
Identities = 319/439 (72%), Positives = 376/439 (85%), Gaps = 9/439 (2%)
Frame = -2
Query: 1291 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 1112
MS + + + P+IL+LLL++ L +RK+ NLPPGNMGWPFLGETIGYLKPYSA + GE
Sbjct: 1 MSHSELVVLLLPSILSLLLVFILMQRKQARFNLPPGNMGWPFLGETIGYLKPYSATSIGE 60
Query: 1111 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 932
FMEQHISR+GKIYKS+LFG TIVS DA LNRFILQ++GRLFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQSEGRLFECSYPRSIGGILGKWSML 120
Query: 931 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 752
VLVGDMH+NMR+ISLNF+S ARLR+HL+++VE LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRNMRSISLNFLSHARLRSHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 751 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 572
MAKH+MS++PGKPE+E+L++EY+TFMKGVVS PLNFPGT YRKALKSRS ILK IEQ M
Sbjct: 181 LMAKHMMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTPYRKALKSRSIILKFIEQKM 240
Query: 571 DEKIRKTNGHSDKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYFL 392
+E+I + + +E+DDLLGWVLKHSNL+TEQILDLILS+LFAGHET+S+SI+LAIYFL
Sbjct: 241 EERIARLKEGVEDLEEDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAIYFL 300
Query: 391 QGCPEAVQELREEHLGITKAKKQAGESGLNWEDYKQMEFTQCVISETLRLGNVVRFLHRK 212
Q CP A+Q+L+EEH+ I++AKKQ+GE +NW+DYK+MEFTQCVISETLRLGNVVRFLHRK
Sbjct: 301 QACPGAIQQLKEEHIEISRAKKQSGEMEINWDDYKKMEFTQCVISETLRLGNVVRFLHRK 360
Query: 211 ALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQK---------GKTSAP 59
A++DVRYKGYDIPCGWKVLPV +AVHLD FDQPQ FNPWRWQ +A
Sbjct: 361 AVRDVRYKGYDIPCGWKVLPVISAVHLDSTVFDQPQQFNPWRWQHNNARGSSTCSSAAAA 420
Query: 58 TSNNFMPFGGGPRLCAGSE 2
+SN+FMPFGGGPRLCAGSE
Sbjct: 421 SSNHFMPFGGGPRLCAGSE 439
>gb|AER08630.1| steroid 22-alpha hydroxylase [Populus euphratica]
Length = 490
Score = 667 bits (1720), Expect = 0.0
Identities = 320/441 (72%), Positives = 374/441 (84%), Gaps = 11/441 (2%)
Frame = -2
Query: 1291 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 1112
MS + + P+IL+LLL++ L +RK+ NLPPGNMGWPFLGETIGYLKPYSA + GE
Sbjct: 1 MSHSELAVLLLPSILSLLLVFILMQRKQARFNLPPGNMGWPFLGETIGYLKPYSATSIGE 60
Query: 1111 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 932
FMEQHISR+GKIYKS+LFG TIVS DA LNRFILQ++GRLFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQSEGRLFECSYPRSIGGILGKWSML 120
Query: 931 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 752
VLVGDMH+NMR ISLNF+S ARLR+HL+++VE LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRNMRNISLNFLSHARLRSHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 751 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 572
MAKH+MS++PGKPE+E+L++EY+TFMKGVVS PLNFPGT YRKALKSRS+ILK IEQ M
Sbjct: 181 LMAKHMMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTPYRKALKSRSTILKFIEQKM 240
Query: 571 DEKIRKTNGHSDKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYFL 392
DE+I + + +E+DDLLGWVLKHSNL+TEQILDLILS+LFAGHET+S+SI+LAIYFL
Sbjct: 241 DERIARLKEGVEDLEEDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAIYFL 300
Query: 391 QGCPEAVQELREEHLGITKAKKQAGESGLNWEDYKQMEFTQCVISETLRLGNVVRFLHRK 212
Q CP A+Q+L+EEH+ I++AKKQ+GE +NW+DYK+MEFTQCVISETLRLGNVVRFLHRK
Sbjct: 301 QACPGAIQQLKEEHIEISRAKKQSGEMEMNWDDYKKMEFTQCVISETLRLGNVVRFLHRK 360
Query: 211 ALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQ-----------KGKTS 65
A++DVRYKGYDIPCGWKVLPV +AVHLD FDQPQ FNPWRWQ +
Sbjct: 361 AVRDVRYKGYDIPCGWKVLPVISAVHLDSTVFDQPQQFNPWRWQHNNARGSSTCSSAAAA 420
Query: 64 APTSNNFMPFGGGPRLCAGSE 2
A +SN FMPFGGGPRLCAGSE
Sbjct: 421 AASSNYFMPFGGGPRLCAGSE 441