BLASTX nr result
ID: Coptis25_contig00021593
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00021593
(1408 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002272243.1| PREDICTED: aspartic proteinase nepenthesin-2... 559 e-157
ref|XP_002312826.1| predicted protein [Populus trichocarpa] gi|2... 520 e-145
ref|XP_002523869.1| Aspartic proteinase nepenthesin-2 precursor,... 508 e-141
ref|XP_002280866.1| PREDICTED: aspartic proteinase nepenthesin-2... 498 e-138
ref|XP_002534234.1| pepsin A, putative [Ricinus communis] gi|223... 494 e-137
>ref|XP_002272243.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
Length = 467
Score = 559 bits (1441), Expect = e-157
Identities = 276/427 (64%), Positives = 332/427 (77%), Gaps = 3/427 (0%)
Frame = +1
Query: 76 SPFSDPFQKLTHLTAASLTRAKHLKNPQTTNISTTPLSPHSYGAYSVSLSFGTPPQTIPL 255
SP DP++ L HL +ASL RA+HLKNP+TT STTPL HSYGAYS+ LSFGTPPQT+PL
Sbjct: 46 SPPPDPYRNLRHLVSASLIRARHLKNPKTTPTSTTPLFTHSYGAYSIPLSFGTPPQTLPL 105
Query: 256 IFDTGSELVWVPCTHRYICRNCTSST-NTQIPTFIPKLSSSTKIIGCKNPKCSWIHTTNV 432
I DTGS+LVW PCTHRY+CRNC+ ST N FIPK SSS+K++GC NPKC WIH + V
Sbjct: 106 IMDTGSDLVWFPCTHRYVCRNCSFSTSNPSSNIFIPKSSSSSKVLGCVNPKCGWIHGSKV 165
Query: 433 VESRCKDCEPNSTICSQICPPYIIIYGSASTGGILLSETLDLVSRKVPNFAVGCSLFASH 612
+SRC+DCEP S C+QICPPY++ YGS TGGI+LSETLDL + VPNF VGCS+ ++
Sbjct: 166 -QSRCRDCEPTSPNCTQICPPYLVFYGSGITGGIMLSETLDLPGKGVPNFIVGCSVLSTS 224
Query: 613 SPAGIAGFGRGSASLPSQLGLSKFSYCLLSHRFDDSTQSSSLVLDGELNNGDSKTNGVSY 792
PAGI+GFGRG SLPSQLGL KFSYCLLS R+DD+T+SSSLVLDGE ++G+ KT G+SY
Sbjct: 225 QPAGISGFGRGPPSLPSQLGLKKFSYCLLSRRYDDTTESSSLVLDGESDSGE-KTAGLSY 283
Query: 793 TPFVKNP-VTGRPAFSVYYYVGLRKITVGDKTVKIPYSKLSQRADGNGGAIVDSGSTFTF 969
TPFV+NP V G+ AFSVYYY+GLR ITVG K VKIPY L ADG+GG I+DSG+TFT+
Sbjct: 284 TPFVQNPKVAGKHAFSVYYYLGLRHITVGGKHVKIPYKYLIPGADGDGGTIIDSGTTFTY 343
Query: 970 MEGHVFEPVAAELEKQTAHYKRLTDVENVTGLRPCYDISGGKPVSFPKLVFHFKGGADME 1149
M+G +FE VAAE EKQ KR T+VE +TGLRPC++ISG SFP+L F+GGA+ME
Sbjct: 344 MKGEIFELVAAEFEKQ-VQSKRATEVEGITGLRPCFNISGLNTPSFPELTLKFRGGAEME 402
Query: 1150 LPVTNYFS-LVGDGIACMTIITDXXXXXXXXXXXXPAVILGNFQMQNFYVEYDLENKRFG 1326
LP+ NY + L GD + C+TI+TD PA+ILGNFQ QNFYVEYDL N+R G
Sbjct: 403 LPLANYVAFLGGDDVVCLTIVTD--GAAGKEFSGGPAIILGNFQQQNFYVEYDLRNERLG 460
Query: 1327 FRQQTCK 1347
FRQQ+CK
Sbjct: 461 FRQQSCK 467
>ref|XP_002312826.1| predicted protein [Populus trichocarpa] gi|222849234|gb|EEE86781.1|
predicted protein [Populus trichocarpa]
Length = 445
Score = 520 bits (1338), Expect = e-145
Identities = 258/430 (60%), Positives = 318/430 (73%), Gaps = 8/430 (1%)
Frame = +1
Query: 79 PFSDPFQKLTHLTAASLTRAKHLKNPQTTNISTT--PLSPHSYGAYSVSLSFGTPPQTIP 252
PF D +QKL HL SL RA+HLKNPQTT +TT PL HSYG YSVSLSFGTPPQT+
Sbjct: 22 PFQDQYQKLNHLVTTSLARARHLKNPQTTPATTTTAPLFSHSYGGYSVSLSFGTPPQTLS 81
Query: 253 LIFDTGSELVWVPCTHRYICRNCT---SSTNTQIPTFIPKLSSSTKIIGCKNPKCSWIHT 423
I DTGS++VW PCT Y+C++C+ SS +++I FIPK SSS+K++GCKNPKCSWIH
Sbjct: 82 FIMDTGSDIVWFPCTSHYLCKHCSFSSSSPSSRIQPFIPKESSSSKLLGCKNPKCSWIHH 141
Query: 424 TNVVESRC-KDCEPNSTICSQICPPYIIIYGSASTGGILLSETLDLVSRKVPNFAVGCSL 600
+N+ C +DC S + +Q CPPY+I YGS +TGG+ LSETL L S PNF VGCS+
Sbjct: 142 SNI---NCDQDCSIKSCL-NQTCPPYMIFYGSGTTGGVALSETLHLHSLSKPNFLVGCSV 197
Query: 601 FASHSPAGIAGFGRGSASLPSQLGLSKFSYCLLSHRFDDSTQ-SSSLVLDGELNNGDSKT 777
F+SH PAGIAGFGRG +SLPSQLGL KFSYCLLSHRFDD T+ SSSLVLD E + D KT
Sbjct: 198 FSSHQPAGIAGFGRGLSSLPSQLGLGKFSYCLLSHRFDDDTKKSSSLVLDMEQLDSDKKT 257
Query: 778 NGVSYTPFVKNP-VTGRPAFSVYYYVGLRKITVGDKTVKIPYSKLSQRADGNGGAIVDSG 954
N + YTPFVKNP V + +FSVYYY+GLR+ITVG VK+PY LS DGNGG I+DSG
Sbjct: 258 NALVYTPFVKNPKVDNKSSFSVYYYLGLRRITVGGHHVKVPYKYLSPGEDGNGGVIIDSG 317
Query: 955 STFTFMEGHVFEPVAAELEKQTAHYKRLTDVENVTGLRPCYDISGGKPVSFPKLVFHFKG 1134
+TFTFM FEP++ E +Q Y+R+ ++E+ GLRPC+++S K VSFP+L +FKG
Sbjct: 318 TTFTFMAREAFEPLSDEFIRQIKDYRRVKEIEDAIGLRPCFNVSDAKTVSFPELRLYFKG 377
Query: 1135 GADMELPVTNYFSLVGDGIACMTIITDXXXXXXXXXXXXPAVILGNFQMQNFYVEYDLEN 1314
GAD+ LPV NYF+ VG +AC+T++TD P +ILGNFQMQNFYVEYDL N
Sbjct: 378 GADVALPVENYFAFVGGEVACLTVVTD--GVAGPERVGGPGMILGNFQMQNFYVEYDLRN 435
Query: 1315 KRFGFRQQTC 1344
+R GF+Q+ C
Sbjct: 436 ERLGFKQEKC 445
>ref|XP_002523869.1| Aspartic proteinase nepenthesin-2 precursor, putative [Ricinus
communis] gi|223536957|gb|EEF38595.1| Aspartic proteinase
nepenthesin-2 precursor, putative [Ricinus communis]
Length = 447
Score = 508 bits (1309), Expect = e-141
Identities = 249/426 (58%), Positives = 314/426 (73%), Gaps = 2/426 (0%)
Frame = +1
Query: 76 SPFSDPFQKLTHLTAASLTRAKHLKNPQTTNISTTPLSPHSYGAYSVSLSFGTPPQTIPL 255
+P D QKL +L + SL RA HLKNPQTT P+ HSYG YS+SLSFGTPPQT+
Sbjct: 38 NPSQDHLQKLNYLVSTSLARAHHLKNPQTT-----PVFSHSYGGYSISLSFGTPPQTLSF 92
Query: 256 IFDTGSELVWVPCTHRYICRNCTSSTNTQIPTFIPKLSSSTKIIGCKNPKCSWIHTTNVV 435
+ DTGS VW PCT RY+C NC S ++I F+PK SSS+KIIGCKNPKCSWIH T++
Sbjct: 93 VMDTGSSFVWFPCTLRYLCNNC--SFTSRISPFLPKHSSSSKIIGCKNPKCSWIHQTDL- 149
Query: 436 ESRCKDCEPNSTICSQICPPYIIIYGSASTGGILLSETLDLVSRKVPNFAVGCSLFASHS 615
RC DC+ NS CSQICPPY+I+YGS +TGG+ LSETL L VPNF VGCS+F+S
Sbjct: 150 --RCTDCDNNSRNCSQICPPYLILYGSGTTGGVALSETLHLHGLIVPNFLVGCSVFSSRQ 207
Query: 616 PAGIAGFGRGSASLPSQLGLSKFSYCLLSHRFDDSTQSSSLVLDGELNNGDSKTNGVSYT 795
PAGIAGFGRG +SLPSQLGL+KFSYCLLSH+FDD+ +SSSLVLD + ++ D KT + YT
Sbjct: 208 PAGIAGFGRGPSSLPSQLGLTKFSYCLLSHKFDDTQESSSLVLDSQ-SDSDKKTAALMYT 266
Query: 796 PFVKNP-VTGRPAFSVYYYVGLRKITVGDKTVKIPYSKLSQRADGNGGAIVDSGSTFTFM 972
P VKNP V +PAFSVYYYV LR+I++G ++VKIPY LS DGNGG I+DSG+TFT+M
Sbjct: 267 PLVKNPKVQDKPAFSVYYYVSLRRISIGGRSVKIPYKYLSPDKDGNGGTIIDSGTTFTYM 326
Query: 973 EGHVFEPVAAELEKQTAHYKRLTDVENVTGLRPCYDISGGKPVSFPKLVFHFKGGADMEL 1152
FE ++ E Q +Y+R VE ++GL+PC+++SG K + P+L HFKGGAD+EL
Sbjct: 327 STEAFEILSNEFISQVKNYERALMVEALSGLKPCFNVSGAKELELPQLRLHFKGGADVEL 386
Query: 1153 PVTNYFSLVGD-GIACMTIITDXXXXXXXXXXXXPAVILGNFQMQNFYVEYDLENKRFGF 1329
P+ NYF+ +G +AC T++TD P +ILGNFQMQNFYVEYDL+N+R GF
Sbjct: 387 PLENYFAFLGSREVACFTVVTD-----GAEKASGPGMILGNFQMQNFYVEYDLQNERLGF 441
Query: 1330 RQQTCK 1347
++++CK
Sbjct: 442 KKESCK 447
>ref|XP_002280866.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
Length = 469
Score = 498 bits (1281), Expect = e-138
Identities = 247/427 (57%), Positives = 306/427 (71%), Gaps = 3/427 (0%)
Frame = +1
Query: 76 SPFSDPFQKLTHLTAASLTRAKHLKNPQTTNISTTPLSPHSYGAYSVSLSFGTPPQTIPL 255
+P SDP+Q L+HLT+ASLTRA HLK+ + T+ TPL HSYG YSVSLSFGTP QT+
Sbjct: 46 NPSSDPWQLLSHLTSASLTRAHHLKHRKNTSSVNTPLFAHSYGGYSVSLSFGTPSQTLSF 105
Query: 256 IFDTGSELVWVPCTHRYICRNCT--SSTNTQIPTFIPKLSSSTKIIGCKNPKCSWIHTTN 429
+ DTGS LVW PCT RY+C C+ + +IPTFIPKLSSS KI+GC NPKC ++ +
Sbjct: 106 VMDTGSSLVWFPCTSRYVCTRCSFPNIDPAKIPTFIPKLSSSAKIVGCLNPKCGFVMDSE 165
Query: 430 VVESRCKDCEPNSTICSQICPPYIIIYGSASTGGILLSETLDLVSRKVPNFAVGCSLFAS 609
V +RC C+ NS C++ CP Y I YG +T G+LL E+L R P+F VGCS+ +S
Sbjct: 166 V-RTRCPGCDQNSANCTKACPTYAIQYGLGTTVGLLLLESLVFAERTEPDFVVGCSILSS 224
Query: 610 HSPAGIAGFGRGSASLPSQLGLSKFSYCLLSHRFDDSTQSSSLVLDGELNNGDSKTNGVS 789
P+GIAGFGRG +SLP Q+GL KFSYCLLSHRFDDS +SS + L ++ D KT G+S
Sbjct: 225 RQPSGIAGFGRGPSSLPKQMGLKKFSYCLLSHRFDDSPKSSKMTLYVGPDSKDDKTGGLS 284
Query: 790 YTPFVKNPVTGRPAFSVYYYVGLRKITVGDKTVKIPYSKLSQRADGNGGAIVDSGSTFTF 969
YTPF KNPV+ AF YYYV LR I VGDK VK+PYS + +DGNGG IVDSGSTFTF
Sbjct: 285 YTPFRKNPVSSNSAFKEYYYVTLRHIIVGDKRVKVPYSFMVAGSDGNGGTIVDSGSTFTF 344
Query: 970 MEGHVFEPVAAELEKQTAHYKRLTDVENVTGLRPCYDISGGKPVSFPKLVFHFKGGADME 1149
ME VFE VA E ++Q A+Y R DVE ++GL+PC+++SG V+ P LVF FKGGA ME
Sbjct: 345 MEKPVFEAVATEFDRQMANYTRAADVEALSGLKPCFNLSGVGSVALPSLVFQFKGGAKME 404
Query: 1150 LPVTNYFSLVGD-GIACMTIITDXXXXXXXXXXXXPAVILGNFQMQNFYVEYDLENKRFG 1326
LPV NYFSLVGD + C+TI+++ P++ILGN+Q QNFY EYDLEN+RFG
Sbjct: 405 LPVANYFSLVGDLSVLCLTIVSN--EAVGSTLSSGPSIILGNYQSQNFYTEYDLENERFG 462
Query: 1327 FRQQTCK 1347
FR+Q CK
Sbjct: 463 FRRQRCK 469
>ref|XP_002534234.1| pepsin A, putative [Ricinus communis] gi|223525662|gb|EEF28148.1|
pepsin A, putative [Ricinus communis]
Length = 468
Score = 494 bits (1272), Expect = e-137
Identities = 243/429 (56%), Positives = 307/429 (71%), Gaps = 7/429 (1%)
Frame = +1
Query: 79 PFSDPFQKLTHLTAASLTRAKHLKNPQTT-NISTTPLSPHSYGAYSVSLSFGTPPQTIPL 255
P SDP++ L HL S++RA HLK+P+T ++ TPL SYG YS+SLS GTP QT+ L
Sbjct: 40 PSSDPWEYLNHLATTSISRAHHLKSPKTNFSLIKTPLFSRSYGGYSMSLSLGTPSQTVKL 99
Query: 256 IFDTGSELVWVPCTHRYICRNCT--SSTNTQIPTFIPKLSSSTKIIGCKNPKCSWIHTTN 429
I DTGS LVW PCT RY+C +C ++ T+IP F+P+LSSS+K+IGCKNPKC+W+ ++
Sbjct: 100 IMDTGSSLVWFPCTSRYVCASCNFPNTDITKIPKFMPRLSSSSKLIGCKNPKCAWVFGSS 159
Query: 430 VVESRCKDCEPNSTICSQICPPYIIIYGSASTGGILLSETLDLVSRKVPNFAVGCSLFAS 609
V +S+C +C P + C+Q CPPYII YG ST G+LLSET++ ++ + +F GCSL ++
Sbjct: 160 V-QSKCHNCNPQAQNCTQACPPYIIQYGLGSTAGLLLSETINFPNKTISDFLAGCSLLST 218
Query: 610 HSPAGIAGFGRGSASLPSQLGLSKFSYCLLSHRFDDSTQSSSLVLDGELNNGDSKTNGVS 789
P GIAGFGR SLP QLGL KFSYCL+S RFDDS SS L+LD + DSKT G+S
Sbjct: 219 RQPEGIAGFGRSQESLPLQLGLKKFSYCLVSRRFDDSPVSSDLILDMGPSTSDSKTTGLS 278
Query: 790 YTPFVKNPVT-GRPAFSVYYYVGLRKITVGDKTVKIPYSKLSQRADGNGGAIVDSGSTFT 966
YTPF KN + PAF YYYV LRKI VG VK+PYS L +DGNGG IVDSGSTFT
Sbjct: 279 YTPFQKNLASQSNPAFQEYYYVMLRKIIVGKTHVKVPYSFLVPGSDGNGGTIVDSGSTFT 338
Query: 967 FMEGHVFEPVAAELEKQTAHYKRLTDVENVTGLRPCYDISGGKPVSFPKLVFHFKGGADM 1146
F+EGHVFE +A E EKQ A+Y T+V+ +TGLRPC+DISG K V P L F FKGGA M
Sbjct: 339 FVEGHVFELLAKEFEKQMANYTVATNVQKLTGLRPCFDISGEKSVVIPDLTFQFKGGAKM 398
Query: 1147 ELPVTNYFSLVGDGIACMTIITD---XXXXXXXXXXXXPAVILGNFQMQNFYVEYDLENK 1317
+LP++NYF+ V G+ C+TI++D PA+ILGNFQ QNFY+EYDLEN
Sbjct: 399 QLPLSNYFAFVDMGVVCLTIVSDNAAALGGDGGVRSSGPAIILGNFQQQNFYIEYDLEND 458
Query: 1318 RFGFRQQTC 1344
RFGF++Q+C
Sbjct: 459 RFGFKEQSC 467