BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00021554
         (1525 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282769.1| PREDICTED: proline dehydrogenase 2, mitochon...   545   e-152
dbj|BAB83948.1| CIG1 [Nicotiana tabacum]                              500   e-139
gb|AAT57674.1| proline oxidase/dehydrogenase 1 [Nicotiana tabacum]    499   e-139
ref|XP_002305291.1| predicted protein [Populus trichocarpa] gi|2...   494   e-137
gb|AAT57675.1| proline oxidase/dehydrogenase 2 [Nicotiana tabacum]    494   e-137

>ref|XP_002282769.1| PREDICTED: proline dehydrogenase 2, mitochondrial [Vitis vinifera]
            gi|298204407|emb|CBI16887.3| unnamed protein product
            [Vitis vinifera]
          Length = 500

 Score =  545 bits (1403), Expect = e-152
 Identities = 284/494 (57%), Positives = 366/494 (74%), Gaps = 6/494 (1%)
 Frame = -1

Query: 1474 MANR-VSTNLQQNIPSFLKSLNSSPSTYTAVTTPLNLTEELENTIKRKPITTP---TSHK 1307
            MANR VS +   N+  F+++L+SSPS+ T V+ PLNL E+ E T    P       +S  
Sbjct: 1    MANRLVSPSKFLNLRCFVRTLHSSPSSITDVS-PLNLAEKPEETTLLSPTFATGATSSTS 59

Query: 1306 TLSLDDGEXXXXXXXXXXXXXXXLNLQMASFDPIVDMGTWVMKSRLMESMVSKKIVLGMV 1127
            T+ L D +               +NL +A+ +P+VD+G WVMKSR++E+ V ++IVLG +
Sbjct: 60   TIDLHDVKGLFSSMSTAKLLRSSVNLHLATLEPMVDVGMWVMKSRVLETAVLREIVLGTI 119

Query: 1126 KHTLYEHFCAGENLDEASKTLQRIWDS-GLRGILDYGLEDATDNPSCDRNLNEFVKVVES 950
            +HT YEHFCAG++++EA  T + IW+S GLRG+L Y LE  TDN + DRNL  F++ VE 
Sbjct: 120  RHTFYEHFCAGQDVEEACWTARTIWNSAGLRGMLVYALEHTTDNATSDRNLEGFIRTVEG 179

Query: 949  TKLLPSSSVSYACMKITAICPISLLKRVSDLLRWEHRDPSFNLPWKHDTFPVLSDSSPFY 770
             K LP SSVS+   K+TAIC I LLKRVSDLLRW+HRDPSF+LPW+ + FP+ +DSSPFY
Sbjct: 180  AKRLPPSSVSFVIAKMTAICSIDLLKRVSDLLRWQHRDPSFHLPWRQNCFPIFADSSPFY 239

Query: 769  HXXXXXXXXXXXXEQDIEKARERLFELSQKCHDTNLPLLVDAEYTTVQPAIDYLTYLAMI 590
            H            E+D++ A +RLF+L QKC + NLPL VDAEYT+VQPAIDYLTY A I
Sbjct: 240  HTLERPDPLTPQEEKDLQLALQRLFKLCQKCVEANLPLSVDAEYTSVQPAIDYLTYSAAI 299

Query: 589  QFNKD-NNPIVYGTIQAYLKDAKDRMIQATDAAKNMGVTIGLKLVRGAYLSSETKLASSL 413
            Q+NKD NNPIVYGTIQAYLKDAK+R++ A  AA  MGV IG KLVRGAY+SSET+LASSL
Sbjct: 300  QYNKDKNNPIVYGTIQAYLKDAKERLLLAVQAADKMGVPIGFKLVRGAYISSETQLASSL 359

Query: 412  GYASPMHGSIQKTHSSYNDCASFMIHKVSTRSAAVLLATHNLESGKMAVKKSQALEIAKG 233
            GY SP+H SI++TH+ +N CASF++ +++  S AV+LATHNLESGK+   K++ L I+K 
Sbjct: 360  GYDSPVHNSIEETHACFNGCASFLLERIAGGSGAVVLATHNLESGKLLATKARDLGISKE 419

Query: 232  NPNLQFAQLKGMAEGLSFGLRNAGFLVSKYLAYGPVEMVIPFLLRRAEENRGLLSASTLD 53
            +  +QFAQL GM+E LSFGLRNAGF VSKY+A+GPVE V+P+LLRRAEENRGLLS STLD
Sbjct: 420  DHKVQFAQLYGMSESLSFGLRNAGFQVSKYMAFGPVEKVMPYLLRRAEENRGLLSTSTLD 479

Query: 52   RQLMRRELVRRLRS 11
            R LMR+EL RR+++
Sbjct: 480  RHLMRKELKRRIKA 493


>dbj|BAB83948.1| CIG1 [Nicotiana tabacum]
          Length = 493

 Score =  500 bits (1287), Expect = e-139
 Identities = 255/494 (51%), Positives = 344/494 (69%), Gaps = 7/494 (1%)
 Frame = -1

Query: 1474 MANRV-STNLQQNIPSFLKSLNSSPSTYTAVTTPLNLTEELENTIKRKPITTPTSH---- 1310
            MAN+V      +++ SF++ LN++P+       P+N T   + T    P   PT      
Sbjct: 1    MANKVVCPKAFRDLRSFVRCLNTAPTV-----PPMNFTGAYDATTVTTPALIPTDQVITA 55

Query: 1309 --KTLSLDDGEXXXXXXXXXXXXXXXLNLQMASFDPIVDMGTWVMKSRLMESMVSKKIVL 1136
              K ++ +D +               L LQMA+ +P+VD+G WVM S+LM   + K+++L
Sbjct: 56   DKKVINFEDVKELFTGVSTLKLIRSTLTLQMAATEPMVDVGIWVMNSKLMHMPIVKEVIL 115

Query: 1135 GMVKHTLYEHFCAGENLDEASKTLQRIWDSGLRGILDYGLEDATDNPSCDRNLNEFVKVV 956
            G VK T YEHFCAG++L E  +T+ ++ D GL+G+LDYG+E AT+N SCD+++  F++  
Sbjct: 116  GFVKGTFYEHFCAGKDLIEVRRTVTKLSDVGLKGMLDYGVEHATENESCDQSMKVFLQTA 175

Query: 955  ESTKLLPSSSVSYACMKITAICPISLLKRVSDLLRWEHRDPSFNLPWKHDTFPVLSDSSP 776
            ESTK LPSSSVS+  +KITAIC   LLKR+SDLLRWEH++PSFNLPWK  + P+ SDSSP
Sbjct: 176  ESTKSLPSSSVSFVVVKITAICTPKLLKRMSDLLRWEHKNPSFNLPWKQKSLPLFSDSSP 235

Query: 775  FYHXXXXXXXXXXXXEQDIEKARERLFELSQKCHDTNLPLLVDAEYTTVQPAIDYLTYLA 596
            FYH            E D++ A ERL  + +KC + ++ LL+DAE T +QPAIDY  Y A
Sbjct: 236  FYHTPQKPEPLTVEEEHDLQLAHERLMTICKKCLELDVDLLIDAEDTAIQPAIDYFAYSA 295

Query: 595  MIQFNKDNNPIVYGTIQAYLKDAKDRMIQATDAAKNMGVTIGLKLVRGAYLSSETKLASS 416
             I+++KD++P+++GTIQAYLKD+K+RM+ A  AA+ MGV +G KLVRGAY+SSE +LAS 
Sbjct: 296  AIKYHKDDDPMIFGTIQAYLKDSKERMVIAKKAAEKMGVPMGFKLVRGAYMSSERELASR 355

Query: 415  LGYASPMHGSIQKTHSSYNDCASFMIHKVSTRSAAVLLATHNLESGKMAVKKSQALEIAK 236
            LG  SP+H SI++TH  +N CA FM+ ++S  S AV+LATHN++SGK+A  K+  L I K
Sbjct: 356  LGVQSPIHDSIEQTHDCFNSCAEFMLDEISNGSGAVVLATHNIDSGKLAASKAIDLGIRK 415

Query: 235  GNPNLQFAQLKGMAEGLSFGLRNAGFLVSKYLAYGPVEMVIPFLLRRAEENRGLLSASTL 56
             +  LQFAQL GMAEGLSFGLRNAGF VSKYL +GPVE V+P+L+RRAEENRGLLS S  
Sbjct: 416  DSQKLQFAQLYGMAEGLSFGLRNAGFQVSKYLPFGPVEQVMPYLIRRAEENRGLLSTSAF 475

Query: 55   DRQLMRRELVRRLR 14
            DRQLMR+EL RR +
Sbjct: 476  DRQLMRKELTRRFK 489


>gb|AAT57674.1| proline oxidase/dehydrogenase 1 [Nicotiana tabacum]
          Length = 499

 Score =  499 bits (1286), Expect = e-139
 Identities = 254/497 (51%), Positives = 350/497 (70%), Gaps = 10/497 (2%)
 Frame = -1

Query: 1474 MANRV-STNLQQNIPSFLKSLNSSPSTYTAVTTPLNLTEELENTIKRKPI---------T 1325
            MAN+V    L +N+   ++ LNS+PS  +AV  PL+ T +   T    P          T
Sbjct: 1    MANKVVCPKLLKNLGFHVRRLNSAPSPLSAVP-PLSFTGDFNATTVTTPNLVDTVPQINT 59

Query: 1324 TPTSHKTLSLDDGEXXXXXXXXXXXXXXXLNLQMASFDPIVDMGTWVMKSRLMESMVSKK 1145
             P  +  ++ DD +               L LQMA+ DP+VDMG WVM S+LME  + ++
Sbjct: 60   VPDKNNIINFDDVKELFYGVPTSKLIRSTLTLQMAAIDPMVDMGMWVMNSKLMEMPIFRE 119

Query: 1144 IVLGMVKHTLYEHFCAGENLDEASKTLQRIWDSGLRGILDYGLEDATDNPSCDRNLNEFV 965
            ++LG VK+T YEHFCAG++L E  +T+ ++ D GL+ +LDYG+E AT+N SC++++  F+
Sbjct: 120  VMLGFVKNTFYEHFCAGKDLTEVRRTVSKLSDGGLKAMLDYGVEHATNNESCEQSMKAFI 179

Query: 964  KVVESTKLLPSSSVSYACMKITAICPISLLKRVSDLLRWEHRDPSFNLPWKHDTFPVLSD 785
            + +ESTK LP SS S+  +KITAIC   LLKR+SDLLRWEH+DPSFNLPWK  T P+ ++
Sbjct: 180  QTIESTKSLPQSSTSFVVVKITAICTPRLLKRMSDLLRWEHKDPSFNLPWKQKTLPLFAE 239

Query: 784  SSPFYHXXXXXXXXXXXXEQDIEKARERLFELSQKCHDTNLPLLVDAEYTTVQPAIDYLT 605
            SSP YH            E+D++ ARERL ++ +KC + ++PLL+DAE TT+QP IDY+ 
Sbjct: 240  SSPLYHTSKKPEPLTVEEERDLQLARERLTKICEKCLEHDVPLLIDAEDTTIQPGIDYMA 299

Query: 604  YLAMIQFNKDNNPIVYGTIQAYLKDAKDRMIQATDAAKNMGVTIGLKLVRGAYLSSETKL 425
            Y A I+++KD++P+++GTIQAYLKDAK+RM  A  AA+ MGV +G KLVRGAY+ SE +L
Sbjct: 300  YSAAIKYHKDDDPLIFGTIQAYLKDAKERMAIAKKAAEKMGVPMGFKLVRGAYMCSEREL 359

Query: 424  ASSLGYASPMHGSIQKTHSSYNDCASFMIHKVSTRSAAVLLATHNLESGKMAVKKSQALE 245
            AS LG+ SP+H SI++TH+ +N CA FMI +++  S AV+LATHN+ESGK+A  K+  + 
Sbjct: 360  ASRLGFQSPIHDSIEQTHACFNSCAEFMIEEIANGSGAVVLATHNIESGKLAATKAIDMG 419

Query: 244  IAKGNPNLQFAQLKGMAEGLSFGLRNAGFLVSKYLAYGPVEMVIPFLLRRAEENRGLLSA 65
            I K +  LQFAQL GMAEGL+FGLRNAGF VSKYL +GPV+ ++ +L+RRAEENRG+LS 
Sbjct: 420  I-KDSQKLQFAQLYGMAEGLTFGLRNAGFQVSKYLPFGPVDQIMHYLMRRAEENRGMLST 478

Query: 64   STLDRQLMRRELVRRLR 14
            S  DRQLMR+EL RRL+
Sbjct: 479  SAFDRQLMRKELSRRLK 495


>ref|XP_002305291.1| predicted protein [Populus trichocarpa] gi|222848255|gb|EEE85802.1|
            predicted protein [Populus trichocarpa]
          Length = 435

 Score =  494 bits (1271), Expect = e-137
 Identities = 244/409 (59%), Positives = 316/409 (77%), Gaps = 1/409 (0%)
 Frame = -1

Query: 1234 NLQMASFDPIVDMGTWVMKSRLMES-MVSKKIVLGMVKHTLYEHFCAGENLDEASKTLQR 1058
            NL +AS  P+VD G WVM SRLME+  + +  ++  V+HT +EHFCAGE++ EA + +++
Sbjct: 24   NLYLASISPMVDFGMWVMNSRLMETDNILRAAMIKTVRHTFFEHFCAGEDVVEAGRCIKK 83

Query: 1057 IWDSGLRGILDYGLEDATDNPSCDRNLNEFVKVVESTKLLPSSSVSYACMKITAICPISL 878
            + +SGLRG+L + +E   DN +CD+NL  F+  V+S   LP SSVS   +KITAICPISL
Sbjct: 84   VNESGLRGMLVFAVEYTGDNDACDQNLKGFLDTVQSAMSLPPSSVSSVVVKITAICPISL 143

Query: 877  LKRVSDLLRWEHRDPSFNLPWKHDTFPVLSDSSPFYHXXXXXXXXXXXXEQDIEKARERL 698
            L+RVSDLLRW+ R PSFNLPWK ++FP+ SDSSP YH            E D++  +ERL
Sbjct: 144  LERVSDLLRWQQRYPSFNLPWKQNSFPLFSDSSPLYHTLKKPEPLTPQEEHDLQLGQERL 203

Query: 697  FELSQKCHDTNLPLLVDAEYTTVQPAIDYLTYLAMIQFNKDNNPIVYGTIQAYLKDAKDR 518
            ++L +K    N+PL VDAE T +QPAIDYLTY A I++NKD+NPIVYGTIQAYLKDAK+R
Sbjct: 204  WKLCEKSVQANIPLTVDAEKTAIQPAIDYLTYSAAIKYNKDDNPIVYGTIQAYLKDAKER 263

Query: 517  MIQATDAAKNMGVTIGLKLVRGAYLSSETKLASSLGYASPMHGSIQKTHSSYNDCASFMI 338
            ++ AT AA  M V +G K+VRGAY+SSE+KLAS+LGY SP+H SIQ+TH+ YNDCASFM+
Sbjct: 264  LLLATKAADKMRVPMGFKVVRGAYMSSESKLASALGYDSPIHNSIQETHACYNDCASFML 323

Query: 337  HKVSTRSAAVLLATHNLESGKMAVKKSQALEIAKGNPNLQFAQLKGMAEGLSFGLRNAGF 158
             K++  S AV+LATHN+ESG++A  K+  L I KGNP L+FAQL GM++ LSFGL NAGF
Sbjct: 324  EKIANSSDAVILATHNVESGRLAATKALDLGIRKGNPKLEFAQLYGMSDALSFGLSNAGF 383

Query: 157  LVSKYLAYGPVEMVIPFLLRRAEENRGLLSASTLDRQLMRRELVRRLRS 11
            LVSKY+ YGPVE VIP+LLRRAEENRGLLS S++D++LMR+EL RRL++
Sbjct: 384  LVSKYMPYGPVEKVIPYLLRRAEENRGLLSTSSIDKELMRKELKRRLKA 432


>gb|AAT57675.1| proline oxidase/dehydrogenase 2 [Nicotiana tabacum]
          Length = 499

 Score =  494 bits (1271), Expect = e-137
 Identities = 252/497 (50%), Positives = 346/497 (69%), Gaps = 10/497 (2%)
 Frame = -1

Query: 1474 MANRV-STNLQQNIPSFLKSLNSSPSTYTAVTTPLNLTEELENTIKRKPI---------T 1325
            MAN+V    L +N+   ++ LNS+PS  + V  PL+ T +   T    P          T
Sbjct: 1    MANKVVCPKLLKNLGFHVRRLNSAPSPLSVVP-PLSFTGDFNATTVTTPNLVDTVPQINT 59

Query: 1324 TPTSHKTLSLDDGEXXXXXXXXXXXXXXXLNLQMASFDPIVDMGTWVMKSRLMESMVSKK 1145
             P  +  ++ DD +               L LQMA+ +P+VDMG WVM S+LME  + ++
Sbjct: 60   VPDKNNIINFDDVKELFYGVPTSKLIRSSLTLQMAAIEPMVDMGMWVMNSKLMEMPIFRE 119

Query: 1144 IVLGMVKHTLYEHFCAGENLDEASKTLQRIWDSGLRGILDYGLEDATDNPSCDRNLNEFV 965
            ++LG VK+T YEHFCAG++L E  +T+ ++ D GL+ +LDYG+E AT N SC++++  F+
Sbjct: 120  VMLGFVKNTFYEHFCAGKDLTEVRRTVSKLSDGGLKAMLDYGVEHATHNESCEQSMKAFI 179

Query: 964  KVVESTKLLPSSSVSYACMKITAICPISLLKRVSDLLRWEHRDPSFNLPWKHDTFPVLSD 785
            + +ESTK LP SS S+  +KITAIC   LLKR+SDLLRWEH+DPSFNLPWK  T P+ ++
Sbjct: 180  QTIESTKSLPQSSTSFVVVKITAICTPRLLKRMSDLLRWEHKDPSFNLPWKQKTLPLFAE 239

Query: 784  SSPFYHXXXXXXXXXXXXEQDIEKARERLFELSQKCHDTNLPLLVDAEYTTVQPAIDYLT 605
            SSP YH            E+D++ A +RL ++ +KC + ++PLL+DAE TT+QP IDY+ 
Sbjct: 240  SSPLYHTSKKPEPLTVEEERDLQLAHKRLTKICEKCLEHDVPLLIDAEDTTIQPGIDYMA 299

Query: 604  YLAMIQFNKDNNPIVYGTIQAYLKDAKDRMIQATDAAKNMGVTIGLKLVRGAYLSSETKL 425
            Y A I+++KD+ P+++GTIQAYLKDAK+RM  A  AA+ MGV +G KLVRGAY+ SE +L
Sbjct: 300  YSAAIKYHKDDGPLIFGTIQAYLKDAKERMAIAKKAAEKMGVPMGFKLVRGAYMCSEREL 359

Query: 424  ASSLGYASPMHGSIQKTHSSYNDCASFMIHKVSTRSAAVLLATHNLESGKMAVKKSQALE 245
            AS LG+ SP+H SI++TH+ +N CA FMI +++  S AV+LATHN+ESGK+A  K+  L 
Sbjct: 360  ASRLGFQSPIHDSIEQTHACFNSCAEFMIEEIANGSGAVVLATHNIESGKLAATKAIDLG 419

Query: 244  IAKGNPNLQFAQLKGMAEGLSFGLRNAGFLVSKYLAYGPVEMVIPFLLRRAEENRGLLSA 65
            I K +  LQFAQL GMAEGLSFGLRNAGF VSKYL +GPV+ ++ +L+RRAEENRG+LS 
Sbjct: 420  I-KDSQKLQFAQLYGMAEGLSFGLRNAGFQVSKYLPFGPVDQIMHYLMRRAEENRGMLST 478

Query: 64   STLDRQLMRRELVRRLR 14
            S  DRQLMR+EL RRL+
Sbjct: 479  SAFDRQLMRKELSRRLK 495