BLASTX nr result
ID: Coptis25_contig00021432
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00021432
(1436 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AER38246.1| PME3 [Gossypium barbadense] 526 e-147
gb|AER38245.1| PME4 [Gossypium barbadense] 521 e-145
emb|CBI29628.3| unnamed protein product [Vitis vinifera] 519 e-145
dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis] 519 e-145
ref|XP_002270616.1| PREDICTED: pectinesterase/pectinesterase inh... 519 e-145
>gb|AER38246.1| PME3 [Gossypium barbadense]
Length = 514
Score = 526 bits (1356), Expect = e-147
Identities = 257/427 (60%), Positives = 322/427 (75%), Gaps = 2/427 (0%)
Frame = +2
Query: 5 ELIWTQSAVEKPIGGNTGTGVLGFDLKTWLSAALGNQDTCMDGFLGTSGSVQNSIAGSLK 184
+L W+ SA + P G + TG + DL+TWLSAA+ NQ+TC++GF GT+G + +AG L
Sbjct: 88 QLSWSLSASQNPNGKHNSTGDVASDLRTWLSAAMANQETCIEGFEGTNGIAKTVVAGGLN 147
Query: 185 NVTTQVKNVLGMVRPVRPRHAYN-ERKLGSTD-FPPWMSANDRRLLEAPTTGLSADVVVA 358
VT+ V ++L MV+P N +RK+ + FP W D++LL+A G++AD VVA
Sbjct: 148 QVTSLVSDLLTMVQPPGSDSRSNGDRKVAEKNRFPSWFEREDQKLLQA--NGVTADAVVA 205
Query: 359 TDGSGNFTSISDAISTAPDNSTHRFVIYIKKGVYRXXXXXXXXXXXLMIVGDGMNSTIVS 538
DG+G FT+I DA++ APD S +R VIYIKKG+Y+ LM+VGDG+N TI+S
Sbjct: 206 LDGTGTFTNIMDAVAAAPDYSMNRHVIYIKKGLYKENVEIKKKKWNLMMVGDGINGTIIS 265
Query: 539 AKLNVIDGCTTFKSATFAVVGIGFIARDIAFENTAGPEKHQAVAYRSDSDLSVLYRCGFY 718
+ +DG TTF+SATFAV G GFIARDI FENTAGP+KHQAVA RSDSDLSV +RC
Sbjct: 266 GNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCAIK 325
Query: 719 GYQDTLYAHSLRQFYRECVITGTVDFIFGNSVVVFQGCRILAKKGLSSQQNIVTAHGRID 898
GYQD+LY H++RQF+REC ITGTVDFIFG++ VVFQ C+ILAK+GL +Q+N +TA GR D
Sbjct: 326 GYQDSLYTHTMRQFFRECRITGTVDFIFGDASVVFQNCQILAKQGLPNQKNTITAQGRKD 385
Query: 899 PDQNTGFSLQFCNISADSDLLRFVNSTPTYLGRPWKQYSRTVFMQSYMTSAIKPKGWLEW 1078
P+Q TGFS+QFCNISAD+DLL FVNSTPTYLGRPWK YSRT+ MQSY+ +A++P+GWLEW
Sbjct: 386 PNQPTGFSIQFCNISADADLLPFVNSTPTYLGRPWKLYSRTIIMQSYIGNAVRPQGWLEW 445
Query: 1079 DGDFALSTLYYGEFLNFGPGAALRGRVNWTGYHAKMTPTQAATFTVAQFIDGNLWLPKTG 1258
+ DFAL TLYY EF+NFGPGA L GRV W GYHA QA FTVA+ I+G+LWLP TG
Sbjct: 446 NQDFALDTLYYAEFMNFGPGAGLGGRVQWPGYHALNNSAQAGNFTVARLIEGDLWLPSTG 505
Query: 1259 VKYTAGL 1279
VKYTAGL
Sbjct: 506 VKYTAGL 512
>gb|AER38245.1| PME4 [Gossypium barbadense]
Length = 525
Score = 521 bits (1343), Expect = e-145
Identities = 259/436 (59%), Positives = 317/436 (72%), Gaps = 12/436 (2%)
Frame = +2
Query: 5 ELIWTQSAVEKPIGGNTGTGVLGFDLKTWLSAALGNQDTCMDGFLGTSGSVQNSIAGSLK 184
EL WT SA + P + TG L DL+TWLSAA+ NQ TC+DGF GT+ V+ ++GSL
Sbjct: 90 ELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMVNQQTCIDGFEGTNSMVKTVVSGSLN 149
Query: 185 NVTTQVKNVLGMVRPVRPRHAYNERKLGS------------TDFPPWMSANDRRLLEAPT 328
+T+ V+N+L MV P P N + GS + FP W DRRLL+
Sbjct: 150 QITSLVRNLLIMVHP-GPNSKSNGTRNGSQKGGGGGGHPGQSRFPVWFKREDRRLLQI-- 206
Query: 329 TGLSADVVVATDGSGNFTSISDAISTAPDNSTHRFVIYIKKGVYRXXXXXXXXXXXLMIV 508
G++A+VVVA DGSGNFT I DA+ TAPD S +R+VIYIKKG+Y+ L+++
Sbjct: 207 NGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYVIYIKKGLYKENVEIKKKKWNLVMI 266
Query: 509 GDGMNSTIVSAKLNVIDGCTTFKSATFAVVGIGFIARDIAFENTAGPEKHQAVAYRSDSD 688
GDGM+ T++S + IDG TT +SATFAV G GFIARDI FENTAGP+KHQAVA RSDSD
Sbjct: 267 GDGMDVTVISGNRSFIDGWTTLRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSD 326
Query: 689 LSVLYRCGFYGYQDTLYAHSLRQFYRECVITGTVDFIFGNSVVVFQGCRILAKKGLSSQQ 868
LSV +RC GYQD+LY H++RQFYREC ITGTVDFIFG+ V+FQ C+ILAK+GL SQ+
Sbjct: 327 LSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDFIFGDGAVLFQNCQILAKQGLPSQK 386
Query: 869 NIVTAHGRIDPDQNTGFSLQFCNISADSDLLRFVNSTPTYLGRPWKQYSRTVFMQSYMTS 1048
N +TA GR DP+Q TGFS+QFCNISAD+DLL VNSTPTYLGRPWK YSRT+ MQSY++
Sbjct: 387 NTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNSTPTYLGRPWKLYSRTIIMQSYISD 446
Query: 1049 AIKPKGWLEWDGDFALSTLYYGEFLNFGPGAALRGRVNWTGYHAKMTPTQAATFTVAQFI 1228
AI+P+GWLEW+ DFAL TLYY E++N GPGA+L RV W GYH QA FTVAQFI
Sbjct: 447 AIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSERVKWPGYHVLNNSAQAVNFTVAQFI 506
Query: 1229 DGNLWLPKTGVKYTAG 1276
+G+LWLP TGVKYT+G
Sbjct: 507 EGDLWLPSTGVKYTSG 522
>emb|CBI29628.3| unnamed protein product [Vitis vinifera]
Length = 527
Score = 519 bits (1336), Expect = e-145
Identities = 253/430 (58%), Positives = 317/430 (73%), Gaps = 5/430 (1%)
Frame = +2
Query: 5 ELIWTQSAVEKPIGGNTGTGVLGFDLKTWLSAALGNQDTCMDGFLGTSGSVQNSIAGSLK 184
+L W+ SA++ P G + GTG LG DLKTWLS+ NQDTC++GF+GT+G V+ +A SL
Sbjct: 98 DLSWSLSAIQNPKGKDNGTGDLGSDLKTWLSSTFTNQDTCIEGFVGTNGIVKTVVAESLS 157
Query: 185 NVTTQVKNVLGMVRPVRPRHAYNE-----RKLGSTDFPPWMSANDRRLLEAPTTGLSADV 349
V + V ++L MV P+ N + +GS DFP W+ + R+LL+A + +S DV
Sbjct: 158 QVASLVHSLLTMVHDPAPKGKSNGGGGGVKHVGSGDFPSWVGKHSRKLLQA--SSVSPDV 215
Query: 350 VVATDGSGNFTSISDAISTAPDNSTHRFVIYIKKGVYRXXXXXXXXXXXLMIVGDGMNST 529
VA DG+GN+T++ DA+ APD S + +VIYIK+G+YR LM+VGDGM +T
Sbjct: 216 TVAADGTGNYTTVMDAVQAAPDYSQNHYVIYIKQGIYRENVEIKKKKWNLMMVGDGMGAT 275
Query: 530 IVSAKLNVIDGCTTFKSATFAVVGIGFIARDIAFENTAGPEKHQAVAYRSDSDLSVLYRC 709
+++ + IDG TT+ SATFAV G GFIARD+ FENTAGPEKHQAVA RSDSDLSV YRC
Sbjct: 276 VITGNRSYIDGWTTYASATFAVKGKGFIARDMTFENTAGPEKHQAVALRSDSDLSVYYRC 335
Query: 710 GFYGYQDTLYAHSLRQFYRECVITGTVDFIFGNSVVVFQGCRILAKKGLSSQQNIVTAHG 889
GYQDTLY H+ RQFYREC I+GTVDFIFG++ VVFQ C+IL KKGL +Q+N +TA G
Sbjct: 336 SMRGYQDTLYPHTNRQFYRECRISGTVDFIFGDATVVFQNCQILVKKGLPNQKNTITAQG 395
Query: 890 RIDPDQNTGFSLQFCNISADSDLLRFVNSTPTYLGRPWKQYSRTVFMQSYMTSAIKPKGW 1069
R DP Q TGFS+QF NISADSDLL VNST +YLGRPWKQYSRT+ M+SY++ AI+P+GW
Sbjct: 396 RKDPAQPTGFSIQFSNISADSDLLASVNSTLSYLGRPWKQYSRTIIMKSYISDAIRPEGW 455
Query: 1070 LEWDGDFALSTLYYGEFLNFGPGAALRGRVNWTGYHAKMTPTQAATFTVAQFIDGNLWLP 1249
LEW+GDFAL TLYYGE++N+GP A L RV W G+H QAA FTV +FI GNLWLP
Sbjct: 456 LEWNGDFALDTLYYGEYMNYGPSAGLGSRVQWPGFHLLNNSAQAANFTVTEFIAGNLWLP 515
Query: 1250 KTGVKYTAGL 1279
TGVKY+AGL
Sbjct: 516 STGVKYSAGL 525
>dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis]
Length = 509
Score = 519 bits (1336), Expect = e-145
Identities = 255/425 (60%), Positives = 314/425 (73%)
Frame = +2
Query: 5 ELIWTQSAVEKPIGGNTGTGVLGFDLKTWLSAALGNQDTCMDGFLGTSGSVQNSIAGSLK 184
EL W+ SA + P G + TG L DL+TWLSAAL NQDTC DGF GT+ V+ + L
Sbjct: 91 ELNWSLSASQNPEGKDNSTGKLSSDLRTWLSAALVNQDTCSDGFEGTNSIVKGLVTTGLN 150
Query: 185 NVTTQVKNVLGMVRPVRPRHAYNERKLGSTDFPPWMSANDRRLLEAPTTGLSADVVVATD 364
VT+ V+ +L V+P H N + P W+ A DR+LL+A G++ D VVA D
Sbjct: 151 QVTSLVQGLLTQVQPNTDHHGPNG------EIPSWVKAEDRKLLQAG--GVNVDAVVAQD 202
Query: 365 GSGNFTSISDAISTAPDNSTHRFVIYIKKGVYRXXXXXXXXXXXLMIVGDGMNSTIVSAK 544
G+GNFT+++DA+ APD S R+VIYIK G Y+ LM++GDGM++TI+S
Sbjct: 203 GTGNFTNVTDAVLAAPDESMTRYVIYIKGGTYKENVEIKKKKWNLMMIGDGMDATIISGS 262
Query: 545 LNVIDGCTTFKSATFAVVGIGFIARDIAFENTAGPEKHQAVAYRSDSDLSVLYRCGFYGY 724
N +DG TTF+SATFAV G GFIARD+ FENTAGPEKHQAVA RSDSDLSV YRC GY
Sbjct: 263 RNFVDGWTTFRSATFAVSGRGFIARDLTFENTAGPEKHQAVALRSDSDLSVFYRCQIRGY 322
Query: 725 QDTLYAHSLRQFYRECVITGTVDFIFGNSVVVFQGCRILAKKGLSSQQNIVTAHGRIDPD 904
QDTLYAH++RQFYR+C I+GTVDFIFG++ VVFQ C+ILAKKGL +Q+N VTA GR DP+
Sbjct: 323 QDTLYAHAMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSVTAQGRKDPN 382
Query: 905 QNTGFSLQFCNISADSDLLRFVNSTPTYLGRPWKQYSRTVFMQSYMTSAIKPKGWLEWDG 1084
+ TG S+QFCNI+AD+DLL FVNST TYLGRPWK YSRTV MQS++++AI+P+GWLEW+
Sbjct: 383 EPTGISIQFCNITADADLLPFVNSTSTYLGRPWKLYSRTVIMQSFLSNAIRPEGWLEWNA 442
Query: 1085 DFALSTLYYGEFLNFGPGAALRGRVNWTGYHAKMTPTQAATFTVAQFIDGNLWLPKTGVK 1264
DFALSTL YGE++N+GPGA L RV W GY TQ +TVAQFI+G+LWLP TGVK
Sbjct: 443 DFALSTLSYGEYINYGPGAGLESRVKWPGYRVFNESTQVQNYTVAQFIEGDLWLPTTGVK 502
Query: 1265 YTAGL 1279
YTAGL
Sbjct: 503 YTAGL 507
>ref|XP_002270616.1| PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B [Vitis
vinifera] gi|15081598|gb|AAK81875.1| pectin
methylesterase PME1 [Vitis vinifera]
Length = 531
Score = 519 bits (1336), Expect = e-145
Identities = 253/430 (58%), Positives = 317/430 (73%), Gaps = 5/430 (1%)
Frame = +2
Query: 5 ELIWTQSAVEKPIGGNTGTGVLGFDLKTWLSAALGNQDTCMDGFLGTSGSVQNSIAGSLK 184
+L W+ SA++ P G + GTG LG DLKTWLS+ NQDTC++GF+GT+G V+ +A SL
Sbjct: 102 DLSWSLSAIQNPKGKDNGTGDLGSDLKTWLSSTFTNQDTCIEGFVGTNGIVKTVVAESLS 161
Query: 185 NVTTQVKNVLGMVRPVRPRHAYNE-----RKLGSTDFPPWMSANDRRLLEAPTTGLSADV 349
V + V ++L MV P+ N + +GS DFP W+ + R+LL+A + +S DV
Sbjct: 162 QVASLVHSLLTMVHDPAPKGKSNGGGGGVKHVGSGDFPSWVGKHSRKLLQA--SSVSPDV 219
Query: 350 VVATDGSGNFTSISDAISTAPDNSTHRFVIYIKKGVYRXXXXXXXXXXXLMIVGDGMNST 529
VA DG+GN+T++ DA+ APD S + +VIYIK+G+YR LM+VGDGM +T
Sbjct: 220 TVAADGTGNYTTVMDAVQAAPDYSQNHYVIYIKQGIYRENVEIKKKKWNLMMVGDGMGAT 279
Query: 530 IVSAKLNVIDGCTTFKSATFAVVGIGFIARDIAFENTAGPEKHQAVAYRSDSDLSVLYRC 709
+++ + IDG TT+ SATFAV G GFIARD+ FENTAGPEKHQAVA RSDSDLSV YRC
Sbjct: 280 VITGNRSYIDGWTTYASATFAVKGKGFIARDMTFENTAGPEKHQAVALRSDSDLSVYYRC 339
Query: 710 GFYGYQDTLYAHSLRQFYRECVITGTVDFIFGNSVVVFQGCRILAKKGLSSQQNIVTAHG 889
GYQDTLY H+ RQFYREC I+GTVDFIFG++ VVFQ C+IL KKGL +Q+N +TA G
Sbjct: 340 SMRGYQDTLYPHTNRQFYRECRISGTVDFIFGDATVVFQNCQILVKKGLPNQKNTITAQG 399
Query: 890 RIDPDQNTGFSLQFCNISADSDLLRFVNSTPTYLGRPWKQYSRTVFMQSYMTSAIKPKGW 1069
R DP Q TGFS+QF NISADSDLL VNST +YLGRPWKQYSRT+ M+SY++ AI+P+GW
Sbjct: 400 RKDPAQPTGFSIQFSNISADSDLLASVNSTLSYLGRPWKQYSRTIIMKSYISDAIRPEGW 459
Query: 1070 LEWDGDFALSTLYYGEFLNFGPGAALRGRVNWTGYHAKMTPTQAATFTVAQFIDGNLWLP 1249
LEW+GDFAL TLYYGE++N+GP A L RV W G+H QAA FTV +FI GNLWLP
Sbjct: 460 LEWNGDFALDTLYYGEYMNYGPSAGLGSRVQWPGFHLLNNSAQAANFTVTEFIAGNLWLP 519
Query: 1250 KTGVKYTAGL 1279
TGVKY+AGL
Sbjct: 520 STGVKYSAGL 529