BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00021314
         (3788 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002524359.1| RNA binding protein, putative [Ricinus commu...   810   0.0  
ref|XP_004163683.1| PREDICTED: uncharacterized LOC101213211 [Cuc...   726   0.0  
ref|XP_004147263.1| PREDICTED: uncharacterized protein LOC101213...   726   0.0  
ref|NP_193496.1| tRNA/rRNA methyltransferase (SpoU) family prote...   699   0.0  
ref|XP_002326676.1| predicted protein [Populus trichocarpa] gi|2...   806   0.0  

>ref|XP_002524359.1| RNA binding protein, putative [Ricinus communis]
            gi|223536320|gb|EEF37970.1| RNA binding protein, putative
            [Ricinus communis]
          Length = 1744

 Score =  810 bits (2091), Expect(3) = 0.0
 Identities = 426/669 (63%), Positives = 507/669 (75%), Gaps = 5/669 (0%)
 Frame = -1

Query: 2039 LRFREQTGAEICLAAYEALVPVLKAVASSMTPKIFDLVMASDRSLMPKAEDRHWLETLII 1860
            L +  ++GAE+CLAAYEAL PVL+A+  + +P   DL+  SD+S    AE++ WL+ L++
Sbjct: 888  LTYDTESGAEVCLAAYEALAPVLRALVFTFSPLALDLIRDSDKS-SSSAEEKAWLDQLVL 946

Query: 1859 CFLENINELLAVGMLARSRRAILMNWKWLCLNSLLSILCWMMENEVHPDNAATFVSTTSL 1680
             FL+NIN LLAVG+L RSRRA+L+NWKWLCL SLLSI  +  EN  H  +   F S  ++
Sbjct: 947  SFLQNINNLLAVGVLVRSRRAVLLNWKWLCLESLLSIPHYAFENGPHLVDNRLFFSEAAI 1006

Query: 1679 NCIFSDLIGSLENANESSVLPMLISVRMVMGLYASERLGPIVFSSDGMKTEMMWDLVRSS 1500
              IFSDL+ SLENA E SVLPML S+R+  GL AS   G +V S +G+  +MMW LVRSS
Sbjct: 1007 RLIFSDLVESLENAGEGSVLPMLRSIRLTFGLLASGNSGSLVSSCNGVDAQMMWHLVRSS 1066

Query: 1499 WILHVSCKKRKVAHIAALLSSVLHFSVFCDDRMHETAENTQGPCKWFIEKIIEEATKSPR 1320
            W+LHVS  KR+VA IAALLSSVLH SVF D+ MH T  N  GP KWF+E I+ E TKSPR
Sbjct: 1067 WMLHVSNNKRRVAAIAALLSSVLHASVFADEAMH-TNNNGPGPLKWFVENILVEGTKSPR 1125

Query: 1319 TIRLAALHLTGLWLMYPRTIRYYVKELKLLSLHGSVXXXXXXXXXXXESHDARTEVSLLA 1140
            TIRLAALHLTGLWL  PR ++YY+KELKLL+L+GSV           E+ DARTEVSLLA
Sbjct: 1126 TIRLAALHLTGLWLSQPRMMKYYIKELKLLTLYGSVAFDEDFEAELAENRDARTEVSLLA 1185

Query: 1139 KGSDIELTEAFMNTELYARVSVAVLFHELANRADRLGSVKENEDRHAALHAGKSFLLELL 960
            K  D ELTEAF+NTELYARVSVAVL + LA+ A+ +GS  ENED  AAL +GK FLLELL
Sbjct: 1186 KCPDSELTEAFINTELYARVSVAVLLNNLADLANLVGSANENEDCSAALESGKIFLLELL 1245

Query: 959  DSVVNDKDLAKELYKKYSGIHRRKVRAWQMICILSRFVDEDIVQQVTASLHITIYRNNMP 780
            DS VNDKDLAKELYKKYSGIHRRK+R WQMIC+LSRFV +DIV +VT SLHI +YRNN+P
Sbjct: 1246 DSAVNDKDLAKELYKKYSGIHRRKIRVWQMICVLSRFVTDDIVGKVTCSLHIALYRNNLP 1305

Query: 779  SIRQYLETFAIQIYLKFPSLVKEQLSPIFQDHNMRPQ-----ALSSYVFIAANVILHTTE 615
            ++RQYLETFAI IYLKFP+LV EQL PI +D++MRPQ     ALSSYVFIAAN+ILHT++
Sbjct: 1306 AVRQYLETFAINIYLKFPTLVGEQLVPILRDYDMRPQVNITSALSSYVFIAANIILHTSK 1365

Query: 614  EIVRFRHLNELLPPITPLLTSHHHSLRGFAQLLVYQVLSKMFPALDCDGSHILSLETRCF 435
               + RHL+ELLPPI PLLTSHHHSLRGF QLLVYQVLSK+   LDC  S    LE RCF
Sbjct: 1366 AF-QSRHLDELLPPILPLLTSHHHSLRGFTQLLVYQVLSKILSPLDCGASETTDLEKRCF 1424

Query: 434  KDLKSYLAKNSDCARLRASMQGFLDAFDPIAFATPIGIFTARDEGFEFECVPTSLMEQVN 255
            +DLKSYLAKN DC RLRASM+G+LDA++PI   TP GIF  R E  EFECVPTSL+E+V 
Sbjct: 1425 EDLKSYLAKNPDCRRLRASMEGYLDAYNPIVSGTPTGIFINRVEELEFECVPTSLLEEVL 1484

Query: 254  LFLNEVREDLRCSMAKDMVTIKNESLIIGDNFNGTNASTTANKEALPSQVPKEISLDFQK 75
             FLN+VREDLRCSMAKD++TIKNES  I +N           +  LP ++ +E SLDFQK
Sbjct: 1485 SFLNDVREDLRCSMAKDVITIKNESFKIDEN--------PTCRRTLPKELLEEASLDFQK 1536

Query: 74   KITLSNHER 48
            KIT S HE+
Sbjct: 1537 KITPSKHEK 1545



 Score =  539 bits (1389), Expect(3) = 0.0
 Identities = 283/562 (50%), Positives = 378/562 (67%), Gaps = 24/562 (4%)
 Frame = -3

Query: 3786 GTQRDAVVSEKDLDENYSTSIGSTRRGQWAEKEAKSLGVGQICNLTDTCSNGQQKWDAFI 3607
            GTQ ++  +EK   E YS   G T+R  WA+KEAKSLGVG++CN  D+  +G+Q+W+AFI
Sbjct: 280  GTQSNSPNTEKKSQEKYSIPNGMTKRAIWADKEAKSLGVGKLCNSMDSPLDGRQQWEAFI 339

Query: 3606 LLYEMLEEYGTHLVEAAWNHQISMLLHIPC-LQRSGNAIGGVHQSQMETLEEMFSWLAIL 3430
            LLYEMLEEYGTHLVEAAW HQ+++LL          N+I G+ Q+Q   L E+FSWL IL
Sbjct: 340  LLYEMLEEYGTHLVEAAWEHQVTLLLQFSVSYDNFANSICGIQQNQTAVLGEVFSWLTIL 399

Query: 3429 WERALCHDNPQVRCLIMQSFLSVEWKNHGNCAEIVPESFIFGPFIQGLNDTVHHREFGVK 3250
            W+    HDNPQVRCLIMQSFL +EW  + + A+ VPESFI G F++GLND VHH++FGVK
Sbjct: 400  WQLGFRHDNPQVRCLIMQSFLGIEWMKYHDAAKSVPESFILGSFVEGLNDPVHHKDFGVK 459

Query: 3249 GFYSSKTIEGATTFVSHFCKHFHLRERIAFMCSLASLVKQESFGRAGLMALSACIASAAC 3070
            G Y+S+TIE A  F+  + ++ + R+ I F+ SLAS+ K +SFGRAGLM L+ CIASAAC
Sbjct: 460  GVYTSRTIEAAARFLCQYTRYLNARKEIVFLHSLASVAKHQSFGRAGLMGLAECIASAAC 519

Query: 3069 GV--------QWCEVCSSDVEHQISFEASLLCNSKTDLLDILRLLIQSCKQHFNLNYRHQ 2914
            GV        +W +    D E  +   +  +  +KTDLLD+LR +I+S KQHFN  YR +
Sbjct: 520  GVGLRDGNEAEWTKDALCD-EVWLDSSSKKVHINKTDLLDVLRYVIESSKQHFNPKYRFR 578

Query: 2913 --------------VCENVVEAASSVICTSDVPLETLMHFISVVPRECTDYDGPLRGKVQ 2776
                          VCE V+ AA+SV+ T DVPLE L+HF+S +PRE TDY GPLR K+Q
Sbjct: 579  VLNTIIVIPFVIFAVCEKVLGAATSVVSTVDVPLEVLLHFVSTLPREFTDYGGPLRVKMQ 638

Query: 2775 NWFSKCSQTLQRSESFSTREHVLHSLCAFPKRYTEYHHSPEAFVTYDDGDLQAWEFEAQR 2596
             W     +     + FS+   +L SL  FP+R+T   H  +AFV++DD DL AW+ E +R
Sbjct: 639  EWLLGVDK-----KHFSSEIQLLKSLQEFPERFTSSQHVVDAFVSFDDEDLDAWDSEVKR 693

Query: 2595 WARVLLLVITTKHHLELICEFLQDCGINICKQNKFMEWLPQKFLILNSSLVQELQIMHMK 2416
            WARVL LVI  ++HL  I +FL++CG++ICKQ K   W P KFL+L  +L+ E++I+  +
Sbjct: 694  WARVLFLVIKEENHLVPIFKFLRNCGVDICKQCKHAGWSPVKFLVLTVNLIAEIRILWER 753

Query: 2415 SIGCGIEVRSNMKSHMLDRPARLGSLDASIIFEKFTFTFLFIL-ELVSYAKSICSAFWSC 2239
             +  G ++RSN ++ +L    +LGS +AS I EKF+  FL IL ELVS+A + CS FW+ 
Sbjct: 754  EVERGFKIRSNSENSLLRTIDQLGSEEASAINEKFSDLFLSILEELVSFASTSCSIFWTS 813

Query: 2238 PNLDSSLPSSVRGKLGGPSQRR 2173
               D+ LPSSVRGKLGGPSQRR
Sbjct: 814  FVKDTDLPSSVRGKLGGPSQRR 835



 Score = 38.5 bits (88), Expect(3) = 0.0
 Identities = 19/48 (39%), Positives = 29/48 (60%)
 Frame = -2

Query: 2173 ILSMKTVASVASWCTRFKRDDDSLDLIFTFLWSFSHKVILSKHFDLDS 2030
            + S+ +VASV SWC+ FK +D  L   ++F+W F  K   S  +D +S
Sbjct: 848  VCSLPSVASVTSWCSLFK-NDVQLKFAWSFMWKFFLKTNSSLTYDTES 894


>ref|XP_004163683.1| PREDICTED: uncharacterized LOC101213211 [Cucumis sativus]
          Length = 1833

 Score =  726 bits (1874), Expect(2) = 0.0
 Identities = 390/684 (57%), Positives = 487/684 (71%), Gaps = 4/684 (0%)
 Frame = -1

Query: 2087 FLVEFLSQGYSVKTL*LRFREQTGAEICLAAYEALVPVLKAVASSMTPKIFDLVMASDRS 1908
            FL+ FL +  S       +  ++GAEICLA YEAL  VL+ + S  + +    V      
Sbjct: 960  FLLTFLLKTVSSPV----YHSESGAEICLATYEALASVLQVLVSEFSSEALRFVQDESTI 1015

Query: 1907 LMPKAEDRHWLETLIICFLENINELLAVGMLARSRRAILMNWKWLCLNSLLSILCWMMEN 1728
              P+ E R  L++LI+ F +++N +L  G+L R+RRA+L+ WKW CL SLLSI    ++N
Sbjct: 1016 HHPRVEGRPLLDSLILTFHQHVNGILDAGVLVRTRRAVLLKWKWHCLESLLSIPYCALQN 1075

Query: 1727 EVHPDNAATFVSTTSLNCIFSDLIGSLENANESSVLPMLISVRMVMGLYASERLGPIVFS 1548
             +  ++   F+S  +L  IF+DL+ SLENA ESSVLPML  VR+++ L+     G +V S
Sbjct: 1076 GISLEDNNAFLSEATLLQIFNDLVESLENAGESSVLPMLRLVRLILCLFYKGNSGLLVTS 1135

Query: 1547 SDGMKTEMMWDLVRSSWILHVSCKKRKVAHIAALLSSVLHFSVFCDDRMHETAENTQGPC 1368
             +G+ +EMMW LV SSWILHVSC KR+VAHIA LLSSVLH S F +  MH  ++   GP 
Sbjct: 1136 CNGVNSEMMWRLVHSSWILHVSCNKRRVAHIAVLLSSVLHSSAFSEINMH-LSDGGPGPL 1194

Query: 1367 KWFIEKIIEEATKSPRTIRLAALHLTGLWLMYPRTIRYYVKELKLLSLHGSVXXXXXXXX 1188
            KWFIEKI+EE TKSPRT RLAALHLTG+WL +P TI+YY+KELKLLSL+GS+        
Sbjct: 1195 KWFIEKILEEGTKSPRTFRLAALHLTGMWLSHPWTIKYYLKELKLLSLYGSI-AFDEDFE 1253

Query: 1187 XXXESHDARTEVSLLAKGSDIELTEAFMNTELYARVSVAVLFHELANRADRLGSVKENED 1008
                 HD +TEVSLLA+  D ELTE F+NTELYARVSVA LFH+LA+ A  +    E   
Sbjct: 1254 AELTDHDTQTEVSLLAESPDPELTEVFINTELYARVSVATLFHKLADLA-MVELSNEYGS 1312

Query: 1007 RHAALHAGKSFLLELLDSVVNDKDLAKELYKKYSGIHRRKVRAWQMICILSRFVDEDIVQ 828
             + A+ +G+ FLLELLDSVVN  DLAKELYKK+S IHRRK+RAWQM+CILSRFV EDI+Q
Sbjct: 1313 CYDAVESGRLFLLELLDSVVNSNDLAKELYKKHSAIHRRKIRAWQMMCILSRFVCEDIIQ 1372

Query: 827  QVTASLHITIYRNNMPSIRQYLETFAIQIYLKFPSLVKEQLSPIFQDHNMRPQ----ALS 660
            QVT SLH+ + +NN+PS+RQYLETFAI IYLKFP+LVKEQL PI QD+NM+PQ     LS
Sbjct: 1373 QVTNSLHVCLSKNNLPSVRQYLETFAISIYLKFPTLVKEQLVPILQDYNMKPQVTISVLS 1432

Query: 659  SYVFIAANVILHTTEEIVRFRHLNELLPPITPLLTSHHHSLRGFAQLLVYQVLSKMFPAL 480
            SYVFIA NVILH  E+ V+  HL+ELLP + P LTSHHHSLRGF QLLVY VL K FPA+
Sbjct: 1433 SYVFIATNVILHANED-VQSSHLDELLPSLVPQLTSHHHSLRGFTQLLVYHVLCKFFPAM 1491

Query: 479  DCDGSHILSLETRCFKDLKSYLAKNSDCARLRASMQGFLDAFDPIAFATPIGIFTARDEG 300
                +  + LE RCF+DLKSYL KN DC RLRASM+G+L A++P++  TP GIF++R + 
Sbjct: 1492 KFRPTGYMPLEKRCFEDLKSYLEKNPDCVRLRASMEGYLHAYNPVSSVTPSGIFSSRVKD 1551

Query: 299  FEFECVPTSLMEQVNLFLNEVREDLRCSMAKDMVTIKNESLIIGDNFNGTNASTTANKEA 120
              FECVPTSLMEQV  FLN+VREDLRCSMA D+  IKNES    +  N    S+  N+E 
Sbjct: 1552 L-FECVPTSLMEQVLNFLNDVREDLRCSMANDLTAIKNESFKTNEGHNLIGISSDINEEN 1610

Query: 119  LPSQVPKEISLDFQKKITLSNHER 48
              S++P   SLDFQKK+TLS HE+
Sbjct: 1611 STSKLPVATSLDFQKKVTLSKHEK 1634



 Score =  480 bits (1235), Expect(2) = 0.0
 Identities = 259/528 (49%), Positives = 344/528 (65%), Gaps = 11/528 (2%)
 Frame = -3

Query: 3723 GSTRRGQWAEKEAKSLGVGQICNLTDTCSNG-QQKWDAFILLYEMLEEYGTHLVEAAWNH 3547
            G T+R +WA KEAKSLGVGQIC+     +N  QQKW+AFILLYEMLEEYG+HLVEAAW+H
Sbjct: 401  GITKRERWANKEAKSLGVGQICSQNKIATNSRQQKWEAFILLYEMLEEYGSHLVEAAWSH 460

Query: 3546 QISMLLHIPCLQRSGNAIGGVHQSQMETLEEMFSWLAILWERALCHDNPQVRCLIMQSFL 3367
            QIS+LL  P      +   GVHQ+Q+E   E++SWL+ILW R   HDNP VRCLIMQ FL
Sbjct: 461  QISLLLQHPTSTEFDSFSSGVHQNQIEMSGEIYSWLSILWVRGFHHDNPLVRCLIMQFFL 520

Query: 3366 SVEWKNHGNCAEIVPESFIFGPFIQGLNDTVHHREFGVKGFYSSKTIEGATTFVSHFCKH 3187
            ++EW++   C + +PE+FI GPFI+ LND V H++FG+KG YSSKT+EGA  FV  +   
Sbjct: 521  TIEWRDKVPCLKSLPETFIIGPFIEALNDPVQHKDFGLKGIYSSKTVEGAARFVCQYTNI 580

Query: 3186 FHLRERIAFMCSLASLVKQESFGRAGLMALSACIASAACGVQW-------CEVCSSDVEH 3028
               R R+ F+  L SL +++SFGR GL++LS CIASAA  V +       C   SS    
Sbjct: 581  LDARTRVVFLHQLTSLARKKSFGRVGLISLSECIASAASIVGFDYNIEGECFNGSSLSSQ 640

Query: 3027 QISFEASLLCNSKTDLLDILRLLIQSCKQHFNLNYRHQVCENVVEAASSVICTSDVPLET 2848
            +     SL C  K +LLD LR +++S KQHFN +Y  QVC   +EAA+SV+CTS++ LE 
Sbjct: 641  EDLIPYSLEC--KLELLDDLRFVVESSKQHFNPSYLLQVCAKALEAAASVLCTSNLALEV 698

Query: 2847 LMHFISVVPRECTDYDGPLRGKVQNWFSKCSQTLQRSESFSTREHVLHSLCAFPKRYTEY 2668
            ++HF+S +PRE TDY G LR K+QNW   C +     +  ST    + SL  FPKR+  +
Sbjct: 699  VLHFVSALPREATDYGGCLRRKMQNWLLGCGK-----KCCSTETKFMKSLIEFPKRFVTH 753

Query: 2667 HHSPEAFVTYDDGDLQAWEFEAQRWARVLLLVITTKHHLELICEFLQDCGINICKQNKFM 2488
            +HS +A VTYDD +L+AWE EA+RWARV+ L +  +HHL+ I  F+ + G+NICKQ   +
Sbjct: 754  NHSSDASVTYDDEELEAWECEAKRWARVVFLAVKKEHHLKSILTFIHNYGLNICKQKGDL 813

Query: 2487 EWLPQKFLILNSSLVQELQIMHMKSIGCGIEVRSNMKSHMLDRPARLGSLDASIIFEKFT 2308
            E +  KFLIL  +LVQELQ++  +   C  + +S      + +P+   S     IF +  
Sbjct: 814  EGIRVKFLILIMTLVQELQLVQQQIGHC--DYKSEFDDLTMSQPSDNLSYAEPTIFSQKI 871

Query: 2307 FTFL--FILELVSYAKSICSAFWSCPNLD-SSLPSSVRGKLGGPSQRR 2173
               L    +ELVS+A   CS FWS    D ++LP SV+GKLGGPSQRR
Sbjct: 872  VNLLPSLQVELVSFATMSCSIFWSNVKSDETTLPGSVKGKLGGPSQRR 919


>ref|XP_004147263.1| PREDICTED: uncharacterized protein LOC101213211 [Cucumis sativus]
          Length = 1833

 Score =  726 bits (1874), Expect(2) = 0.0
 Identities = 390/684 (57%), Positives = 487/684 (71%), Gaps = 4/684 (0%)
 Frame = -1

Query: 2087 FLVEFLSQGYSVKTL*LRFREQTGAEICLAAYEALVPVLKAVASSMTPKIFDLVMASDRS 1908
            FL+ FL +  S       +  ++GAEICLA YEAL  VL+ + S  + +    V      
Sbjct: 960  FLLTFLLKTVSSPV----YHSESGAEICLATYEALASVLQVLVSEFSSEALRFVQDESTI 1015

Query: 1907 LMPKAEDRHWLETLIICFLENINELLAVGMLARSRRAILMNWKWLCLNSLLSILCWMMEN 1728
              P+ E R  L++LI+ F +++N +L  G+L R+RRA+L+ WKW CL SLLSI    ++N
Sbjct: 1016 HHPRVEGRPLLDSLILTFHQHVNGILDAGVLVRTRRAVLLKWKWHCLESLLSIPYCALQN 1075

Query: 1727 EVHPDNAATFVSTTSLNCIFSDLIGSLENANESSVLPMLISVRMVMGLYASERLGPIVFS 1548
             +  ++   F+S  +L  IF+DL+ SLENA ESSVLPML  VR+++ L+     G +V S
Sbjct: 1076 GISLEDNNAFLSEATLLQIFNDLVESLENAGESSVLPMLRLVRLILCLFYKGNSGLLVTS 1135

Query: 1547 SDGMKTEMMWDLVRSSWILHVSCKKRKVAHIAALLSSVLHFSVFCDDRMHETAENTQGPC 1368
             +G+ +EMMW LV SSWILHVSC KR+VAHIA LLSSVLH S F +  MH  ++   GP 
Sbjct: 1136 CNGVNSEMMWRLVHSSWILHVSCNKRRVAHIAVLLSSVLHSSAFSEINMH-LSDGGPGPL 1194

Query: 1367 KWFIEKIIEEATKSPRTIRLAALHLTGLWLMYPRTIRYYVKELKLLSLHGSVXXXXXXXX 1188
            KWFIEKI+EE TKSPRT RLAALHLTG+WL +P TI+YY+KELKLLSL+GS+        
Sbjct: 1195 KWFIEKILEEGTKSPRTFRLAALHLTGMWLSHPWTIKYYLKELKLLSLYGSI-AFDEDFE 1253

Query: 1187 XXXESHDARTEVSLLAKGSDIELTEAFMNTELYARVSVAVLFHELANRADRLGSVKENED 1008
                 HD +TEVSLLA+  D ELTE F+NTELYARVSVA LFH+LA+ A  +    E   
Sbjct: 1254 AELTDHDTQTEVSLLAESPDPELTEVFINTELYARVSVATLFHKLADLA-MVELSNEYGS 1312

Query: 1007 RHAALHAGKSFLLELLDSVVNDKDLAKELYKKYSGIHRRKVRAWQMICILSRFVDEDIVQ 828
             + A+ +G+ FLLELLDSVVN  DLAKELYKK+S IHRRK+RAWQM+CILSRFV EDI+Q
Sbjct: 1313 CYDAVESGRLFLLELLDSVVNSNDLAKELYKKHSAIHRRKIRAWQMMCILSRFVCEDIIQ 1372

Query: 827  QVTASLHITIYRNNMPSIRQYLETFAIQIYLKFPSLVKEQLSPIFQDHNMRPQ----ALS 660
            QVT SLH+ + +NN+PS+RQYLETFAI IYLKFP+LVKEQL PI QD+NM+PQ     LS
Sbjct: 1373 QVTNSLHVCLSKNNLPSVRQYLETFAISIYLKFPTLVKEQLVPILQDYNMKPQVTISVLS 1432

Query: 659  SYVFIAANVILHTTEEIVRFRHLNELLPPITPLLTSHHHSLRGFAQLLVYQVLSKMFPAL 480
            SYVFIA NVILH  E+ V+  HL+ELLP + P LTSHHHSLRGF QLLVY VL K FPA+
Sbjct: 1433 SYVFIATNVILHANED-VQSSHLDELLPSLVPQLTSHHHSLRGFTQLLVYHVLCKFFPAM 1491

Query: 479  DCDGSHILSLETRCFKDLKSYLAKNSDCARLRASMQGFLDAFDPIAFATPIGIFTARDEG 300
                +  + LE RCF+DLKSYL KN DC RLRASM+G+L A++P++  TP GIF++R + 
Sbjct: 1492 KFRPTGYMPLEKRCFEDLKSYLEKNPDCVRLRASMEGYLHAYNPVSSVTPSGIFSSRVKD 1551

Query: 299  FEFECVPTSLMEQVNLFLNEVREDLRCSMAKDMVTIKNESLIIGDNFNGTNASTTANKEA 120
              FECVPTSLMEQV  FLN+VREDLRCSMA D+  IKNES    +  N    S+  N+E 
Sbjct: 1552 L-FECVPTSLMEQVLNFLNDVREDLRCSMANDLTAIKNESFKTNEGHNLIGISSDINEEN 1610

Query: 119  LPSQVPKEISLDFQKKITLSNHER 48
              S++P   SLDFQKK+TLS HE+
Sbjct: 1611 STSKLPVATSLDFQKKVTLSKHEK 1634



 Score =  483 bits (1242), Expect(2) = 0.0
 Identities = 260/528 (49%), Positives = 345/528 (65%), Gaps = 11/528 (2%)
 Frame = -3

Query: 3723 GSTRRGQWAEKEAKSLGVGQICNLTDTCSNG-QQKWDAFILLYEMLEEYGTHLVEAAWNH 3547
            G T+R +WA KEAKSLGVGQIC+     +N  QQKW+AFILLYEMLEEYG+HLVEAAW+H
Sbjct: 401  GITKRERWANKEAKSLGVGQICSQNKIATNSRQQKWEAFILLYEMLEEYGSHLVEAAWSH 460

Query: 3546 QISMLLHIPCLQRSGNAIGGVHQSQMETLEEMFSWLAILWERALCHDNPQVRCLIMQSFL 3367
            QIS+LL  P      +   GVHQ+Q+E   E++SWL+ILW R   HDNP VRCLIMQ FL
Sbjct: 461  QISLLLQHPTSTEFDSFSSGVHQNQIEMSGEIYSWLSILWVRGFHHDNPLVRCLIMQFFL 520

Query: 3366 SVEWKNHGNCAEIVPESFIFGPFIQGLNDTVHHREFGVKGFYSSKTIEGATTFVSHFCKH 3187
            ++EW++   C + +PE+FI GPFI+ LND V H++FG+KG YSSKT+EGA  FV  +   
Sbjct: 521  TIEWRDKVPCLKSLPETFIIGPFIEALNDPVQHKDFGLKGIYSSKTVEGAARFVCQYTNI 580

Query: 3186 FHLRERIAFMCSLASLVKQESFGRAGLMALSACIASAACGVQW-------CEVCSSDVEH 3028
               R R+ F+  L SL +++SFGR GL++LS CIASAA  V +       C   SS    
Sbjct: 581  LDARTRVVFLHQLTSLARKKSFGRVGLISLSECIASAASIVGFDYNIEGECFNGSSLSSQ 640

Query: 3027 QISFEASLLCNSKTDLLDILRLLIQSCKQHFNLNYRHQVCENVVEAASSVICTSDVPLET 2848
            +     SL C  K +LLD LR +++S KQHFN +YR QVC   +EAA+SV+CTS++ LE 
Sbjct: 641  EDLIPYSLEC--KLELLDDLRFVVESSKQHFNPSYRLQVCAKALEAAASVLCTSNLALEV 698

Query: 2847 LMHFISVVPRECTDYDGPLRGKVQNWFSKCSQTLQRSESFSTREHVLHSLCAFPKRYTEY 2668
            ++HF+S +PRE TDY G LR K+QNW   C +     +  ST    + SL  FPKR+  +
Sbjct: 699  VLHFVSALPREATDYGGCLRRKMQNWLLGCGK-----KCCSTETKFMKSLIEFPKRFVTH 753

Query: 2667 HHSPEAFVTYDDGDLQAWEFEAQRWARVLLLVITTKHHLELICEFLQDCGINICKQNKFM 2488
            +HS +A VTYDD +L+AWE EA+RWARV+ L +  +HHL+ I  F+ + G+NICKQ   +
Sbjct: 754  NHSSDASVTYDDEELEAWECEAKRWARVVFLAVKKEHHLKSILTFIHNYGLNICKQKGDL 813

Query: 2487 EWLPQKFLILNSSLVQELQIMHMKSIGCGIEVRSNMKSHMLDRPARLGSLDASIIFEKFT 2308
            E +  KFLIL  +LVQELQ++  +   C  + +S      + +P+   S     IF +  
Sbjct: 814  EGIRVKFLILIMTLVQELQLVQQQIGHC--DYKSEFDDLTMSQPSDNLSYAEPTIFSQKI 871

Query: 2307 FTFL--FILELVSYAKSICSAFWSCPNLD-SSLPSSVRGKLGGPSQRR 2173
               L    +ELVS+A   CS FWS    D ++LP SV+GKLGGPSQRR
Sbjct: 872  VNLLPSLQVELVSFATMSCSIFWSNVKSDETTLPGSVKGKLGGPSQRR 919


>ref|NP_193496.1| tRNA/rRNA methyltransferase (SpoU) family protein [Arabidopsis
            thaliana] gi|332658520|gb|AEE83920.1| tRNA/rRNA
            methyltransferase (SpoU) family protein [Arabidopsis
            thaliana]
          Length = 1850

 Score =  699 bits (1803), Expect(3) = 0.0
 Identities = 378/669 (56%), Positives = 479/669 (71%), Gaps = 10/669 (1%)
 Frame = -1

Query: 2024 QTGAEICLAAYEALVPVLKAVASSMTPKIFDLVMASDRSLMPKAEDRHWLETLIICFLEN 1845
            +  AEI LAA+EAL  VL A  S  +   F+L + +D +L+   +   WL+  +  F+ N
Sbjct: 993  EAAAEIYLAAFEALASVLNAFVSLCSAGAFNL-LENDSTLLSMVDGEFWLQVSVPAFVRN 1051

Query: 1844 INELLAVGMLARSRRAILMNWKWLCLNSLLSILCWMMENEVHPDNAATFVSTTSLNCIFS 1665
            IN LL  G+L RSRRA+L++WKWLC+ SLLS++  +    + P++  +F S  ++  IF 
Sbjct: 1052 INHLLTAGVLVRSRRAVLLSWKWLCVESLLSVMHILDARRI-PEDRKSFFSDDTVKSIFQ 1110

Query: 1664 DLIGSLENANESSVLPMLISVRMVMGLYASERLGPIVFSSDGMKTEMMWDLVRSSWILHV 1485
            D++ SLENA E S LPML SVR+ +G+ AS +     FS  G+ T+ MW LV+S WILH+
Sbjct: 1111 DIVESLENAGEGSALPMLKSVRLALGILASGKSSLDGFS--GVDTQTMWQLVKSCWILHI 1168

Query: 1484 SCKKRKVAHIAALLSSVLHFSVFCDDRMHETAENTQGPCKWFIEKIIEEATKSPRTIRLA 1305
            SCKKR+VA IAALLSSVLH S+F +  MH T E+  GP KWF+EK++EE  KSPRTIRLA
Sbjct: 1169 SCKKRRVAPIAALLSSVLHSSLFSNKDMHIT-EDEHGPLKWFVEKVLEEGQKSPRTIRLA 1227

Query: 1304 ALHLTGLWLMYPRTIRYYVKELKLLSLHGSVXXXXXXXXXXXESHDARTEVSLLAKGSDI 1125
            ALHL+GLWLMYPRTI+YY+KEL+LL+L+GSV           +++DARTEVSLLAK  D 
Sbjct: 1228 ALHLSGLWLMYPRTIKYYIKELRLLTLYGSVAFDEDFEAELSDNNDARTEVSLLAKSPDP 1287

Query: 1124 ELTEAFMNTELYARVSVAVLFHELANRADRLGSVKENEDRHAALHAGKSFLLELLDSVVN 945
            ELTE F+NTELYARVSVA LF +LAN A  +    +N+D   AL AGK FLLELLD+ V+
Sbjct: 1288 ELTELFINTELYARVSVAGLFQKLANLAYMVEPASQNQDCQDALVAGKLFLLELLDAAVH 1347

Query: 944  DKDLAKELYKKYSGIHRRKVRAWQMICILSRFVDEDIVQQVTASLHITIY--------RN 789
            DKDLAKELYKKYS IHRRK+RAWQMICI+SRFV  DIV QV  S+HI ++        RN
Sbjct: 1348 DKDLAKELYKKYSAIHRRKIRAWQMICIMSRFVCNDIVGQVMDSVHICLHVSLQEQTERN 1407

Query: 788  NMPSIRQYLETFAIQIYLKFPSLVKEQLSPIFQDHNMRPQA--LSSYVFIAANVILHTTE 615
            N+P++RQYLETFAI IYLKFP+LVKEQL PI ++++ + Q     + + + ANVILH  E
Sbjct: 1408 NLPAVRQYLETFAINIYLKFPALVKEQLVPILKNYDSKAQVNLRKTKMIVFANVILH-AE 1466

Query: 614  EIVRFRHLNELLPPITPLLTSHHHSLRGFAQLLVYQVLSKMFPALDCDGSHILSLETRCF 435
            +I +  HL ELLPPI PLLTSHHHSLRGFAQLLV++VL ++FP ++   SH +SLE   F
Sbjct: 1467 KIAQQTHLRELLPPILPLLTSHHHSLRGFAQLLVHRVLFRLFPPVESTSSHTISLEKLSF 1526

Query: 434  KDLKSYLAKNSDCARLRASMQGFLDAFDPIAFATPIGIFTARDEGFEFECVPTSLMEQVN 255
            ++LKSYL KN DC+RLRASM+GFLDA+DP   ATP G+F  R E  EFECVPT LM+ V 
Sbjct: 1527 ENLKSYLDKNPDCSRLRASMEGFLDAYDPSTSATPAGVFVNRVEESEFECVPTCLMDNVL 1586

Query: 254  LFLNEVREDLRCSMAKDMVTIKNESLIIGDNFNGTNASTTANKEALPSQVPKEISLDFQK 75
             FLN+VREDLR SMAKD+VTIKNE   I +        +T ++E L     +  SLDFQ+
Sbjct: 1587 SFLNDVREDLRASMAKDVVTIKNEGFKIDEEPKRRLIMSTTDEERL----SEPSSLDFQR 1642

Query: 74   KITLSNHER 48
            KITLS HE+
Sbjct: 1643 KITLSKHEK 1651



 Score =  379 bits (973), Expect(3) = 0.0
 Identities = 227/570 (39%), Positives = 332/570 (58%), Gaps = 39/570 (6%)
 Frame = -3

Query: 3765 VSEKDLDENYSTSIGSTRRGQWAEKEAKSLGVGQICNLTDTCSNGQQKWDAFILLYEMLE 3586
            +SEK  + N S +   TR+  WAEKEAKSLGVG++    D+    QQ W AF+LLYEMLE
Sbjct: 376  ISEKKPEGN-SVNRSMTRKETWAEKEAKSLGVGELYGSVDSGLTSQQGWQAFLLLYEMLE 434

Query: 3585 EYGTHLVEAAWNHQISMLLHIPCLQRSGNA---IGGVHQSQMETLEE---MFSWLAILWE 3424
            EYGTHLVEAAW++QI +L+    L+  G         H   MET +E   +F+WL +LW 
Sbjct: 435  EYGTHLVEAAWSNQIDLLIK-SSLRYDGTLKSDCNNSHHGHMETPDEEAKIFNWLEVLWN 493

Query: 3423 RALCHDNPQVRCLIMQSFLSVEWKNHGNCAEIVPESFIFGPFIQGLNDTVHHREFGVKGF 3244
            R   HDNP VRC +M+SF  +EW+ +  C + + ++F+ GPFI+GLND  HH++FG+KG 
Sbjct: 494  RGFRHDNPLVRCTVMESFFGIEWRRYKTCTQSMSQTFVLGPFIEGLNDPTHHKDFGLKGI 553

Query: 3243 YSSKTIEGATTFVSHFCKHFHLRERIAFMCSLASLVKQESFGRAGLMALSACIASAACGV 3064
            Y+S+TIEGA  +VS +    + R R+ F+ +LASL K++SF RAG MAL  CI S A  V
Sbjct: 554  YTSRTIEGAAQYVSAYTSCLNPRNRVGFLINLASLAKKQSFCRAGFMALVQCIVSTAYVV 613

Query: 3063 -QWCEVCSSDVEHQISFEAS------LLCNSKTDLLDILRLLIQSCKQHFNLNYRHQ--- 2914
              + +     +E + S  A       L  +  T +LD+L+ + +S +QHFN  YR +   
Sbjct: 614  GGYGDKEMGHLEDKFSGTAQESSCGHLSQDDMTHILDVLKFVAESSRQHFNHKYRIRASL 673

Query: 2913 ------------------VCENVVEAASSVICTSDVPLETLMHFISVVPRECTDYDGPLR 2788
                              V + V+E A+SV+   +VPL TL+ F+S +PRE TD+DG LR
Sbjct: 674  TILNTLKFLLLIVLFHFLVYQKVLETAASVVNPCNVPLGTLLQFVSAIPREFTDHDGLLR 733

Query: 2787 GKVQNWFSKCSQTLQRSESFST-REHVLHSLCAFPKRYTEYHHSPEAFVTYDDGDLQAWE 2611
              +  W   C++  + S S  T    +L SL  + K +T      +   ++DD DL+AW+
Sbjct: 734  KMMLEWLQGCNR--KTSNSLCTDGTRLLASLYEYLKGFTS-----DNVESFDDEDLEAWD 786

Query: 2610 FEAQRWARVLLLVITTKHHLELICEFLQDCGINICKQNKFMEWLPQKFLILNSSLVQELQ 2431
             + +RWARV  L+I  + HL  I  F+Q+ G++  ++   ++  P KFLI   S++ ELQ
Sbjct: 787  SQTKRWARVFFLMINKEEHLTDIIMFVQNNGLSFFQEKNHLKRAPAKFLIFILSMLLELQ 846

Query: 2430 IMH--MKSIGCGIEVRSNMKSHMLDRPARLGSLDASIIFEKFTFTFLFIL-ELVSYAKSI 2260
             M   +  +   ++ +S + S   ++  +   +DAS I +KF    L IL EL+ +A S 
Sbjct: 847  NMQDGISELSSSVKSKSGIGSD--EQTGKQIVVDASSIKKKFAVVLLSILKELIPFADSS 904

Query: 2259 CSAFWSCPNLDS-SLPSSVRGKLGGPSQRR 2173
            CS FWS   +++ +LP SV GKLGGPSQRR
Sbjct: 905  CSIFWSHTTVENGALPGSVIGKLGGPSQRR 934



 Score = 34.7 bits (78), Expect(3) = 0.0
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = -2

Query: 2173 ILSMKTVASVASWCTRFKRDDDSLDLIFTFLWSFSHKVILSK 2048
            +LS+KT+  ++S+C +F      L L   F W F+   I S+
Sbjct: 947  VLSVKTIGLISSYCAQFTSGVGELKLALAFFWKFTQHTISSQ 988


>ref|XP_002326676.1| predicted protein [Populus trichocarpa] gi|222833998|gb|EEE72475.1|
            predicted protein [Populus trichocarpa]
          Length = 1224

 Score =  806 bits (2083), Expect(3) = 0.0
 Identities = 424/663 (63%), Positives = 506/663 (76%)
 Frame = -1

Query: 2024 QTGAEICLAAYEALVPVLKAVASSMTPKIFDLVMASDRSLMPKAEDRHWLETLIICFLEN 1845
            + GAEICLAAYEAL PVL+A+ S+ +    DL+  +D    P  E +  L++L + FL+N
Sbjct: 376  EAGAEICLAAYEALAPVLRALVSTSSSLSLDLIRENDEFSAPVVEGKCCLDSLALSFLQN 435

Query: 1844 INELLAVGMLARSRRAILMNWKWLCLNSLLSILCWMMENEVHPDNAATFVSTTSLNCIFS 1665
            IN LLAVG+LAR+RRA+L+N KW+CL SLLSI      N ++ ++ + F S +++ CIFS
Sbjct: 436  INNLLAVGVLARTRRAVLLNQKWICLESLLSIPYSAPWNVLNLEDGSLFFSDSAIRCIFS 495

Query: 1664 DLIGSLENANESSVLPMLISVRMVMGLYASERLGPIVFSSDGMKTEMMWDLVRSSWILHV 1485
            DL+ SL+NA E SVLPML SVR+ +GL AS +L   V S +G+  +MMW LV SSWILHV
Sbjct: 496  DLVESLDNAGEGSVLPMLRSVRLALGLIASGKLDSHVSSCNGVDAQMMWRLVNSSWILHV 555

Query: 1484 SCKKRKVAHIAALLSSVLHFSVFCDDRMHETAENTQGPCKWFIEKIIEEATKSPRTIRLA 1305
            +C KR+VA IAALLSSVLH SVF D+ MH    N  GP KWF+E +IEE TKSPRTIRLA
Sbjct: 556  NCNKRRVASIAALLSSVLHRSVFTDEGMH-LINNRPGPLKWFVENVIEEGTKSPRTIRLA 614

Query: 1304 ALHLTGLWLMYPRTIRYYVKELKLLSLHGSVXXXXXXXXXXXESHDARTEVSLLAKGSDI 1125
            ALHLTGLWL +P+TI+YY+KELKLLSL+GSV           ++ DA TEVSLLAK  D 
Sbjct: 615  ALHLTGLWLSHPKTIKYYMKELKLLSLYGSVAFDEDFEAELCDNQDASTEVSLLAKSPDP 674

Query: 1124 ELTEAFMNTELYARVSVAVLFHELANRADRLGSVKENEDRHAALHAGKSFLLELLDSVVN 945
            ELTEAF+NTELYARVSVAVLF++LA+ A+ +GS  ENED HAAL +GK FL ELLDS VN
Sbjct: 675  ELTEAFINTELYARVSVAVLFYKLADLANLVGSANENEDCHAALESGKLFLQELLDSAVN 734

Query: 944  DKDLAKELYKKYSGIHRRKVRAWQMICILSRFVDEDIVQQVTASLHITIYRNNMPSIRQY 765
            DKDLAKELYKKYSGIHRRK+RAWQMIC+LSRFV +DIV QVT SLHI++YRNN P++RQY
Sbjct: 735  DKDLAKELYKKYSGIHRRKIRAWQMICVLSRFVTDDIVAQVTHSLHISLYRNNFPAVRQY 794

Query: 764  LETFAIQIYLKFPSLVKEQLSPIFQDHNMRPQALSSYVFIAANVILHTTEEIVRFRHLNE 585
            LETFAI IYLKFP LV+EQL PI +D+NM+PQALSSYVFIAANVILH +    + RH NE
Sbjct: 795  LETFAINIYLKFPLLVREQLVPILRDYNMKPQALSSYVFIAANVILHAS-NANQSRHFNE 853

Query: 584  LLPPITPLLTSHHHSLRGFAQLLVYQVLSKMFPALDCDGSHILSLETRCFKDLKSYLAKN 405
            LLPPI PLLTSHHHSLRGF QLLVYQV  K FP LD  G+  + LE  CF+DLKSYLAKN
Sbjct: 854  LLPPIIPLLTSHHHSLRGFTQLLVYQVFCKYFPMLDY-GASEMPLEKMCFEDLKSYLAKN 912

Query: 404  SDCARLRASMQGFLDAFDPIAFATPIGIFTARDEGFEFECVPTSLMEQVNLFLNEVREDL 225
             DC RLRAS++G+LDA++PIA  TP GIF  R E   FECVPTSLME+V  FLN+VREDL
Sbjct: 913  PDCRRLRASLEGYLDAYNPIASGTPAGIFIDRVEELGFECVPTSLMEEVLNFLNDVREDL 972

Query: 224  RCSMAKDMVTIKNESLIIGDNFNGTNASTTANKEALPSQVPKEISLDFQKKITLSNHERH 45
            RCSMAKD+VTIKNESL        T+      +  + SQ+PKE S DFQKK+TLS HE+ 
Sbjct: 973  RCSMAKDVVTIKNESL-------KTDEDGNCRRTVIDSQLPKETSFDFQKKLTLSKHEKQ 1025

Query: 44   YDD 36
              D
Sbjct: 1026 DTD 1028



 Score =  258 bits (658), Expect(3) = 0.0
 Identities = 145/319 (45%), Positives = 193/319 (60%), Gaps = 8/319 (2%)
 Frame = -3

Query: 3105 MALSACIASAACGV-------QWCEVCSSDVEHQISFEASLLCNSKTDLLDILRLLIQSC 2947
            M L+ CIASAA GV       +W E    D E Q+        + +T  LD+LR +I+S 
Sbjct: 1    MGLAECIASAAHGVGRHDSGAKWSEDAFPD-EVQVESSPENFSDCRTAFLDVLRFVIESS 59

Query: 2946 KQHFNLNYRHQVCENVVEAASSVICTSDVPLETLMHFISVVPRECTDYDGPLRGKVQNWF 2767
            KQHFN  YR QVCE V+EAA+S++ T DVPLE L+HFI+ +PR  TDY G LR K Q W 
Sbjct: 60   KQHFNPYYRLQVCEKVLEAATSLVSTLDVPLEILLHFIATLPRAFTDYGGSLRLKTQEWL 119

Query: 2766 SKCSQTLQRSESFSTREHVLHSLCAFPKRYTEYHHSPEAFVTYDDGDLQAWEFEAQRWAR 2587
               +              +L +L  FP+R+T   +  + F++ DD DL AWE E++RWAR
Sbjct: 120  LGSATEHCNVNCCGAEIQLLKNLQDFPERFTSSQYLVDGFLSLDDEDLDAWESESKRWAR 179

Query: 2586 VLLLVITTKHHLELICEFLQDCGINICKQNKFMEWLPQKFLILNSSLVQELQIMHMKSIG 2407
             L L+I  +  L  I  F+Q+CG+NICKQ   +EWLP KFL+L  SLV E+QIM  +S  
Sbjct: 180  ALFLIIKGEDQLAPILRFIQNCGVNICKQQSHLEWLPVKFLVLARSLVAEIQIMQERSAQ 239

Query: 2406 CGIEVRSNMKSHMLDRPARLGSLDASIIFEKFTFTFLFIL-ELVSYAKSICSAFWSCPNL 2230
            CGI+++   +  +LD   +L   +AS+I  +    FLFIL ELVS+A    S FWS    
Sbjct: 240  CGIKIKCRSEISLLDTVDQLCYTEASMINGRIHGLFLFILEELVSFADLSSSIFWSSITK 299

Query: 2229 DSSLPSSVRGKLGGPSQRR 2173
            +++LP SVRGKLGG SQRR
Sbjct: 300  ETTLPGSVRGKLGGRSQRR 318



 Score = 40.4 bits (93), Expect(3) = 0.0
 Identities = 19/48 (39%), Positives = 29/48 (60%)
 Frame = -2

Query: 2173 ILSMKTVASVASWCTRFKRDDDSLDLIFTFLWSFSHKVILSKHFDLDS 2030
            I S++ VAS++SWC +FK  D  L  ++ FLW F  K + S   D ++
Sbjct: 331  ITSIQAVASISSWCAQFK-SDVKLSSVWNFLWKFFWKTVSSPTCDSEA 377