BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00021152
         (1358 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             450   e-124
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   442   e-122
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              442   e-122
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...   422   e-115
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]                  421   e-115

>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  450 bits (1158), Expect = e-124
 Identities = 226/386 (58%), Positives = 278/386 (72%), Gaps = 4/386 (1%)
 Frame = +1

Query: 1    SNDMIHDDNCQK----EVVNALKFTYDANIERLENRRNIEYYNLDSLRILKASYRCSNVD 168
            SN  IH D   K    EV +ALKF Y A++ERL NRR IE YN ++ R+LK +Y  SN+ 
Sbjct: 395  SNGSIHADGLNKYVSQEVDDALKFPYHASLERLSNRRAIENYNKNNTRVLKTAYSSSNIG 454

Query: 169  NRDIVQFGVEDYNFCQEILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFPPE 348
            N D +   VED+N CQ I ++EL  L RW+ ENRLDKLKFAR KL Y YFS A  +F PE
Sbjct: 455  NEDFLNLAVEDFNICQSIQREELKDLARWITENRLDKLKFARQKLAYCYFSAAATLFSPE 514

Query: 349  LSDARMSWTKNSFLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQA 528
            LSDAR+SW KN  L+TVVDDFFDV GS EELVNLI LVEKW+ + +T+ CSE VEIIF A
Sbjct: 515  LSDARISWAKNGVLTTVVDDFFDVGGSVEELVNLIQLVEKWDVDVSTDCCSENVEIIFSA 574

Query: 529  LHKTVNDLGDIAFTRQGRSVRSHLIDIWLNLLKSMMKEYEWARDKSAPKMEEYMENACIS 708
            LH T+ D  D   T QGR+V SH+IDIWLNLLKSM+KE EW RDKS P M+EYM N  +S
Sbjct: 575  LHSTICDFADKGLTLQGRNVISHIIDIWLNLLKSMLKEAEWLRDKSVPSMDEYMTNGYVS 634

Query: 709  FALGPVVLITQYFLEPTLSKKVITSPEYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSV 888
            FALGP+VL   Y + P LS++++ +PE + L++ M+ C RLLNDIQ+FKRE EEGKLN+V
Sbjct: 635  FALGPIVLPALYCVGPKLSEEIVGTPELHHLYEIMSTCGRLLNDIQTFKRESEEGKLNAV 694

Query: 889  LLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGSIVPKGCKDLFWKTFRVTHL 1068
             LC IH      ++E ++E+K  I   RRELL LVL  KGS+VP+ CKDLFWK  +V HL
Sbjct: 695  SLCMIHGGGDCTKEETIKELKSFIAGKRRELLKLVLQEKGSVVPRACKDLFWKMIKVLHL 754

Query: 1069 FYREKDGFSSSLEMVNAVNAVIHEPL 1146
            FY + DGF+S  EM N+VNAV+ EP+
Sbjct: 755  FYMKDDGFTSH-EMFNSVNAVLEEPI 779


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
            vinifera]
          Length = 793

 Score =  442 bits (1137), Expect = e-122
 Identities = 219/386 (56%), Positives = 283/386 (73%), Gaps = 4/386 (1%)
 Frame = +1

Query: 1    SNDMIHDDNCQK----EVVNALKFTYDANIERLENRRNIEYYNLDSLRILKASYRCSNVD 168
            SN  IH D   K    EV +AL+F Y AN++R+ NRR+IE+YN+D  RILK +YR S+V 
Sbjct: 404  SNSSIHADRLNKYIAQEVEDALRFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVC 463

Query: 169  NRDIVQFGVEDYNFCQEILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFPPE 348
            N+D ++  VED+NFCQ I Q EL  LERW+ ENRLDKLKFAR KL Y YFS A  +F PE
Sbjct: 464  NKDFLKLAVEDFNFCQSIHQNELKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPE 523

Query: 349  LSDARMSWTKNSFLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQA 528
             SDAR+SW KNS L+TVVDDFFD+ GS EEL+NLI LVEKW+ + A + CSE+VEI+F A
Sbjct: 524  QSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSA 583

Query: 529  LHKTVNDLGDIAFTRQGRSVRSHLIDIWLNLLKSMMKEYEWARDKSAPKMEEYMENACIS 708
            LH T++++G  A   Q R+V SH+IDIWL LL+SM++E +W  +KSAP M+EYM NA +S
Sbjct: 584  LHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEYMTNAYVS 643

Query: 709  FALGPVVLITQYFLEPTLSKKVITSPEYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSV 888
            FALGP+VL   YF+ P LS++V+  PE +KL+K M+ C RLLNDI SFKRE +EGK N++
Sbjct: 644  FALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLMSTCGRLLNDIHSFKRESKEGKANAL 703

Query: 889  LLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGSIVPKGCKDLFWKTFRVTHL 1068
             L  IH      E++A+RE+KG++   RREL  LVL  KGS VP+ CKDLFWK  +V H 
Sbjct: 704  ALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLVLQEKGSTVPRICKDLFWKMSKVLHT 763

Query: 1069 FYREKDGFSSSLEMVNAVNAVIHEPL 1146
            FY + DGF+S  +M+ AV +VI+EP+
Sbjct: 764  FYEKDDGFTSH-DMLRAVKSVIYEPV 788


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  442 bits (1137), Expect = e-122
 Identities = 219/386 (56%), Positives = 283/386 (73%), Gaps = 4/386 (1%)
 Frame = +1

Query: 1    SNDMIHDDNCQK----EVVNALKFTYDANIERLENRRNIEYYNLDSLRILKASYRCSNVD 168
            SN  IH D   K    EV +AL+F Y AN++R+ NRR+IE+YN+D  RILK +YR S+V 
Sbjct: 437  SNSSIHADRLNKYIAQEVEDALRFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVC 496

Query: 169  NRDIVQFGVEDYNFCQEILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFPPE 348
            N+D ++  VED+NFCQ I Q EL  LERW+ ENRLDKLKFAR KL Y YFS A  +F PE
Sbjct: 497  NKDFLKLAVEDFNFCQSIHQNELKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPE 556

Query: 349  LSDARMSWTKNSFLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQA 528
             SDAR+SW KNS L+TVVDDFFD+ GS EEL+NLI LVEKW+ + A + CSE+VEI+F A
Sbjct: 557  QSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSA 616

Query: 529  LHKTVNDLGDIAFTRQGRSVRSHLIDIWLNLLKSMMKEYEWARDKSAPKMEEYMENACIS 708
            LH T++++G  A   Q R+V SH+IDIWL LL+SM++E +W  +KSAP M+EYM NA +S
Sbjct: 617  LHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEYMTNAYVS 676

Query: 709  FALGPVVLITQYFLEPTLSKKVITSPEYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSV 888
            FALGP+VL   YF+ P LS++V+  PE +KL+K M+ C RLLNDI SFKRE +EGK N++
Sbjct: 677  FALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLMSTCGRLLNDIHSFKRESKEGKANAL 736

Query: 889  LLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGSIVPKGCKDLFWKTFRVTHL 1068
             L  IH      E++A+RE+KG++   RREL  LVL  KGS VP+ CKDLFWK  +V H 
Sbjct: 737  ALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLVLQEKGSTVPRICKDLFWKMSKVLHT 796

Query: 1069 FYREKDGFSSSLEMVNAVNAVIHEPL 1146
            FY + DGF+S  +M+ AV +VI+EP+
Sbjct: 797  FYEKDDGFTSH-DMLRAVKSVIYEPV 821


>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 808

 Score =  422 bits (1085), Expect = e-115
 Identities = 212/376 (56%), Positives = 273/376 (72%), Gaps = 1/376 (0%)
 Frame = +1

Query: 28   CQKEVV-NALKFTYDANIERLENRRNIEYYNLDSLRILKASYRCSNVDNRDIVQFGVEDY 204
            C  EVV NAL   Y A++ERL NRR+IE YN+D  RILKASYRC N  N+  ++  VED+
Sbjct: 361  CITEVVHNALNCPYYADLERLTNRRSIENYNVDETRILKASYRCLNTGNQHFLKLAVEDF 420

Query: 205  NFCQEILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFPPELSDARMSWTKNS 384
            N CQ I Q+EL  L RWV E RL+KLKFAR KL Y YFS A  +F PELSDAR+SW KN 
Sbjct: 421  NLCQLIHQEELQQLGRWVVEKRLNKLKFARQKLGYCYFSAAATLFAPELSDARLSWAKNG 480

Query: 385  FLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQALHKTVNDLGDIA 564
             L+TVVDDFFDV GS EEL+NLI L+EKW+ + +T++CSE+VEIIF AL  T++++GD A
Sbjct: 481  VLTTVVDDFFDVGGSVEELINLIQLIEKWDVDESTHFCSEQVEIIFSALRSTISEIGDKA 540

Query: 565  FTRQGRSVRSHLIDIWLNLLKSMMKEYEWARDKSAPKMEEYMENACISFALGPVVLITQY 744
            FT QGR V SH+I IWL+LLKSM+ E  W + KS P ++EYM N  +SFALGP+VL   +
Sbjct: 541  FTWQGRKVTSHVIKIWLDLLKSMLTETLWTKSKSIPTLDEYMINGYVSFALGPIVLPALF 600

Query: 745  FLEPTLSKKVITSPEYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSVLLCKIHSPEAIN 924
             + P L+++ +  PE + LFK M  C RLLND + F+RE +EGKLN+V L  I     +N
Sbjct: 601  LVGPKLTEEDVRDPELHDLFKAMGTCGRLLNDWRGFQRESKEGKLNAVSLHMIQGNGGVN 660

Query: 925  EQEAVREVKGIIDRNRRELLGLVLNTKGSIVPKGCKDLFWKTFRVTHLFYREKDGFSSSL 1104
            E+EA+R++KG+I+  R ELL LVL  K S +P+ CKDLFWK  +V HLFY + DGF+S+ 
Sbjct: 661  EEEAIRKIKGLINSQRSELLRLVLREKNSNIPRACKDLFWKMIKVLHLFYLKDDGFTSN- 719

Query: 1105 EMVNAVNAVIHEPLSF 1152
            EM++  NAVI EP++F
Sbjct: 720  EMISTANAVITEPVAF 735


>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
          Length = 737

 Score =  421 bits (1081), Expect = e-115
 Identities = 217/386 (56%), Positives = 270/386 (69%), Gaps = 4/386 (1%)
 Frame = +1

Query: 1    SNDMIHDDNCQK----EVVNALKFTYDANIERLENRRNIEYYNLDSLRILKASYRCSNVD 168
            SN +I      K    EV +ALK    A + RL  R  I+YYN DS RILK+ YRC N+ 
Sbjct: 349  SNTLIRGHKLNKHIGLEVDDALKLPRYAILGRLSTRTAIKYYNTDSTRILKSYYRCLNIG 408

Query: 169  NRDIVQFGVEDYNFCQEILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFPPE 348
            N D ++  V+D N CQ I ++EL  L RWV+E RLDKL FAR K  Y YFS A  +FPPE
Sbjct: 409  NEDFLKLAVDDLNNCQSIHREELNHLTRWVEEYRLDKLNFARQKQAYCYFSAAATLFPPE 468

Query: 349  LSDARMSWTKNSFLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQA 528
            LSDAR+SW KN  L+TVVDDFFDV GS EEL+NLI LVEKW+ N   + CSE VEIIF A
Sbjct: 469  LSDARISWAKNGVLTTVVDDFFDVGGSEEELINLIQLVEKWDVNVGVDCCSENVEIIFSA 528

Query: 529  LHKTVNDLGDIAFTRQGRSVRSHLIDIWLNLLKSMMKEYEWARDKSAPKMEEYMENACIS 708
            L  T+N++G  AFTRQGRSV SH+I+IWL+L+KSM KE EW R+KS P MEEYMENA +S
Sbjct: 529  LKNTINEIGARAFTRQGRSVTSHVIEIWLDLIKSMFKEAEWLRNKSVPTMEEYMENAYVS 588

Query: 709  FALGPVVLITQYFLEPTLSKKVITSPEYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSV 888
            FALGP+VL   Y + P LS++ + S E++ L++ M+   RLLNDIQ FKRE  EGKLN+V
Sbjct: 589  FALGPIVLPALYLVGPKLSEEAVGSSEFDHLYRLMSTAGRLLNDIQGFKRESAEGKLNAV 648

Query: 889  LLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGSIVPKGCKDLFWKTFRVTHL 1068
             L  IH    + E+EA+ E+K +I   RRELL LVL  KGS+VP+ CKDLFW   +V HL
Sbjct: 649  SLAMIHG-NGVTEEEAINEMKNVIVSKRRELLRLVLLEKGSVVPRACKDLFWNMSKVLHL 707

Query: 1069 FYREKDGFSSSLEMVNAVNAVIHEPL 1146
            FY + DGF++  +M+  VNAV+ EP+
Sbjct: 708  FYAKHDGFTAH-DMMKTVNAVMEEPI 732