BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00020734
         (1583 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002269566.1| PREDICTED: E3 ubiquitin-protein ligase HERC2...   265   3e-68
ref|XP_003613573.1| RCC1 and BTB domain-containing protein [Medi...   260   6e-67
emb|CBI32027.3| unnamed protein product [Vitis vinifera]              260   6e-67
ref|XP_003519776.1| PREDICTED: probable E3 ubiquitin-protein lig...   258   3e-66
ref|XP_003517766.1| PREDICTED: probable E3 ubiquitin-protein lig...   250   8e-64

>ref|XP_002269566.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Vitis vinifera]
          Length = 400

 Score =  265 bits (676), Expect = 3e-68
 Identities = 144/237 (60%), Positives = 163/237 (68%)
 Frame = +3

Query: 75  GMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANG 254
           GMRH+LVLLKG   D +YGFGSGKRGQLG SMD  T+S+++PQ   G + V I  I ANG
Sbjct: 160 GMRHSLVLLKG---DQVYGFGSGKRGQLGVSMDN-TRSISVPQATVGLEGVEIISIDANG 215

Query: 255 DQSAALTVDGHLYTWGKGFHGISDACLPLLPSSALKFTKVALGWNHALLLTDCGTVFMLG 434
           D SAAL+ +GHLYTWG+GF   SD   P    S+L FT+VALGWNH L+LT  G VFMLG
Sbjct: 216 DHSAALSANGHLYTWGRGFGSTSDVHCPRCLPSSLCFTQVALGWNHMLVLTGGGEVFMLG 275

Query: 435 GNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSA 614
           GN HG+L  PQK       S       G E+  R   LEKV GLDG+  V IAAGAEHSA
Sbjct: 276 GNRHGVLGDPQKGSQVKHLSVIEVFLHGPEES-REENLEKVQGLDGLNVVQIAAGAEHSA 334

Query: 615 LVTENGKIMTWGWGEHGQLGLGNEEDQIAPQIVSLDAKLLKVQTLFKVYCGSGFTFA 785
           LVTEN  I TWGWGEHGQLGLGN  DQI+PQ V L  +L    T+ KVYCGSGFTFA
Sbjct: 335 LVTENRVIKTWGWGEHGQLGLGNTYDQISPQAVRLGHRLPGQHTMIKVYCGSGFTFA 391



 Score = 65.9 bits (159), Expect = 3e-08
 Identities = 62/224 (27%), Positives = 94/224 (41%), Gaps = 23/224 (10%)
 Frame = +3

Query: 123 LYGFGSGKRGQLGTSM---DMITKSLNLPQVIFGFQQVN-IARIIANGDQSAALTVDGHL 290
           ++ +G+G  GQLGT+    + + + L+LP+    F     I+ +   G    ALT DG +
Sbjct: 14  IWSWGAGTDGQLGTTRLEDEHLPQLLDLPR----FSSAGPISLLACGGAHVLALTTDGKV 69

Query: 291 YTWGKGFHGI---SDACLPLLPS-----SALKFTKVALGWNHALLLTDCGTVFMLGGNHH 446
            TWG+G  G     D    L P       +   T V+ GW+H+  ++D G +F  G    
Sbjct: 70  VTWGRGTSGQLGHGDMVSSLQPKHVKLLESFVMTHVSAGWSHSGFVSDTGCLFTCGDGSF 129

Query: 447 GMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSALVTE 626
           G L          C S+ S                KVS  D      IA G  HS ++ +
Sbjct: 130 GQLGHGD------CKSHCSP--------------VKVSYFDSKHVDQIACGMRHSLVLLK 169

Query: 627 NGKIMTWGWGEHGQLG--LGNEEDQIAPQ---------IVSLDA 725
             ++  +G G+ GQLG  + N      PQ         I+S+DA
Sbjct: 170 GDQVYGFGSGKRGQLGVSMDNTRSISVPQATVGLEGVEIISIDA 213


>ref|XP_003613573.1| RCC1 and BTB domain-containing protein [Medicago truncatula]
            gi|355514908|gb|AES96531.1| RCC1 and BTB
            domain-containing protein [Medicago truncatula]
          Length = 615

 Score =  260 bits (665), Expect = 6e-67
 Identities = 140/237 (59%), Positives = 164/237 (69%)
 Frame = +3

Query: 75   GMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANG 254
            GMRH+LVLLKG   + +YGFGSGKRGQLG S D I KS+NLP+VI GF+   I  I ANG
Sbjct: 388  GMRHSLVLLKGCLSNQVYGFGSGKRGQLGVSKDKI-KSINLPKVISGFEDEEIVGISANG 446

Query: 255  DQSAALTVDGHLYTWGKGFHGISDACLPLLPSSALKFTKVALGWNHALLLTDCGTVFMLG 434
            D SAAL+VDGHLYTWG+GF G  D+ LP   +S  KFTKV LGWNHAL +T  G V+MLG
Sbjct: 447  DHSAALSVDGHLYTWGRGFKGYEDSHLPQCLNSTSKFTKVTLGWNHALAMTGEGKVYMLG 506

Query: 435  GNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSA 614
            GNH G+LS     R              S  D R + LEK  GLDG K  DIA GAEHS 
Sbjct: 507  GNHLGVLSDLHNARPAK-----------SLFDSREANLEKAPGLDGTKITDIATGAEHSV 555

Query: 615  LVTENGKIMTWGWGEHGQLGLGNEEDQIAPQIVSLDAKLLKVQTLFKVYCGSGFTFA 785
            LVTENG+I TWGWGEHGQLGLG++ D+I+P  V+L   L +  ++ +VYCGSGFTFA
Sbjct: 556  LVTENGEIKTWGWGEHGQLGLGDDGDRISPVTVNLGYDLNEAASI-RVYCGSGFTFA 611


>emb|CBI32027.3| unnamed protein product [Vitis vinifera]
          Length = 405

 Score =  260 bits (665), Expect = 6e-67
 Identities = 141/240 (58%), Positives = 165/240 (68%), Gaps = 3/240 (1%)
 Frame = +3

Query: 75  GMRHTLVLLKGN--SGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIA 248
           GMRH+LVLLKG   SGD +YGFGSGKRGQLG SMD  T+S+++PQ   G + V I  I A
Sbjct: 168 GMRHSLVLLKGTGLSGDQVYGFGSGKRGQLGVSMDN-TRSISVPQATVGLEGVEIISIDA 226

Query: 249 NGDQSAALTVDGHLYTWGKGFHGISDACLPLLPSSALKFTKVALGWNHALLLTDCGTVFM 428
           NGD SAAL+ +GHLYTWG+GF   SD   P    S+L FT+VALGWNH L+LT  G VFM
Sbjct: 227 NGDHSAALSANGHLYTWGRGFGSTSDVHCPRCLPSSLCFTQVALGWNHMLVLTGGGEVFM 286

Query: 429 LGGNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEH 608
           LGGN HG+L  PQK           +  +   ++ R   LEKV GLDG+  V IAAGAEH
Sbjct: 287 LGGNRHGVLGDPQK----------GSQVKHLSEESREENLEKVQGLDGLNVVQIAAGAEH 336

Query: 609 SALVTENGKIM-TWGWGEHGQLGLGNEEDQIAPQIVSLDAKLLKVQTLFKVYCGSGFTFA 785
           SALVT N  ++ TWGWGEHGQLGLGN  DQI+PQ V L  +L    T+ KVYCGSGFTFA
Sbjct: 337 SALVTGNILVIKTWGWGEHGQLGLGNTYDQISPQAVRLGHRLPGQHTMIKVYCGSGFTFA 396



 Score = 69.7 bits (169), Expect = 2e-09
 Identities = 63/229 (27%), Positives = 97/229 (42%), Gaps = 51/229 (22%)
 Frame = +3

Query: 123 LYGFGSGKRGQLGTSM---DMITKSLNLPQVIFGFQQVN-IARIIANGDQSAALTVDGHL 290
           ++ +G+G  GQLGT+    + + + L+LP+    F     I+ +   G    ALT DG +
Sbjct: 22  IWSWGAGTDGQLGTTRLEDEHLPQLLDLPR----FSSAGPISLLACGGAHVLALTTDGKV 77

Query: 291 YTWGKGFH---GISDACLPLLPS-----SALKFTKVALGWNHALLLTDCGTVFMLGGNHH 446
            TWG+G     G  D    L P       +   T V+ GW+H+  ++D G +F  G    
Sbjct: 78  VTWGRGTSGQLGHGDMVSSLQPKHVKLLESFVMTHVSAGWSHSGFVSDTGCLFTCGDGSF 137

Query: 447 GMLS--------SPQKLRL--------KTCSSNQS------TGCQG-------------- 518
           G L         SP K+            C    S      TG  G              
Sbjct: 138 GQLGHGDCKSHCSPVKVSYFDSKHVDQIACGMRHSLVLLKGTGLSGDQVYGFGSGKRGQL 197

Query: 519 --SEDDCR-LSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMTWGWG 656
             S D+ R +S+ +   GL+G++ + I A  +HSA ++ NG + TWG G
Sbjct: 198 GVSMDNTRSISVPQATVGLEGVEIISIDANGDHSAALSANGHLYTWGRG 246


>ref|XP_003519776.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Glycine
           max]
          Length = 388

 Score =  258 bits (659), Expect = 3e-66
 Identities = 137/237 (57%), Positives = 167/237 (70%)
 Frame = +3

Query: 75  GMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANG 254
           GMRH+LVLLKGN    +YGFGSGKRGQLG S D + KS+N+P+V+ GF+ V I    ANG
Sbjct: 164 GMRHSLVLLKGNQ---VYGFGSGKRGQLGVSNDRV-KSVNVPKVVSGFEGVEIVGTAANG 219

Query: 255 DQSAALTVDGHLYTWGKGFHGISDACLPLLPSSALKFTKVALGWNHALLLTDCGTVFMLG 434
           D SAA++VDGH+YTWG+GF G  DA +P   +S+L FTKVALGWNHAL ++  G V+MLG
Sbjct: 220 DHSAAVSVDGHVYTWGRGFKGFEDAHVPQCLNSSLNFTKVALGWNHALAMSGEGEVYMLG 279

Query: 435 GNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSA 614
           GNH G+LS  Q +           G      D R   LEKV GLDG K  DIAAGAEHS 
Sbjct: 280 GNHLGVLSDLQNI-----------GPAKHFPDLREFNLEKVPGLDGTKITDIAAGAEHSV 328

Query: 615 LVTENGKIMTWGWGEHGQLGLGNEEDQIAPQIVSLDAKLLKVQTLFKVYCGSGFTFA 785
           +VTE+G+I TWGWGEHGQLGLG+  DQ +P  VSLD  L +  ++ +V+CGSGFTFA
Sbjct: 329 IVTEHGEIKTWGWGEHGQLGLGDTRDQTSPVTVSLDYDLNEAASI-RVFCGSGFTFA 384



 Score = 72.8 bits (177), Expect = 2e-10
 Identities = 56/216 (25%), Positives = 94/216 (43%), Gaps = 11/216 (5%)
 Frame = +3

Query: 102 KGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVD 281
           +  S   ++ +G+G  GQLGT    I +  + PQ++  F   +I+ +   G    ALT  
Sbjct: 13  RDESEQRVWSWGAGTEGQLGTK---ILQDEHFPQLLHQFSLSSISSLACGGAHVIALTSA 69

Query: 282 GHLYTWGKGFHG-------ISDACLPLLPSSALKF--TKVALGWNHALLLTDCGTVFMLG 434
           G   +WG+G  G       +S+   P   +S   +  T V+ GW+H+  ++D G VF  G
Sbjct: 70  GKALSWGRGNSGQLGHGEVVSNTLYPKAVTSLDGYFITHVSAGWSHSGFVSDTGFVFTCG 129

Query: 435 GNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSA 614
               G L                    G  D+       KVS    +    +A G  HS 
Sbjct: 130 DGSFGQL--------------------GHGDNASHCSPVKVSCFVDLHVAQVACGMRHSL 169

Query: 615 LVTENGKIMTWGWGEHGQLGLGNE--EDQIAPQIVS 716
           ++ +  ++  +G G+ GQLG+ N+  +    P++VS
Sbjct: 170 VLLKGNQVYGFGSGKRGQLGVSNDRVKSVNVPKVVS 205


>ref|XP_003517766.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2-like [Glycine
           max]
          Length = 388

 Score =  250 bits (638), Expect = 8e-64
 Identities = 135/237 (56%), Positives = 165/237 (69%)
 Frame = +3

Query: 75  GMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANG 254
           GMRH+LVLLKGN    +YGFGSGKRGQLG S D + KS+N+P+V+ GF+ V IA I ANG
Sbjct: 164 GMRHSLVLLKGNQ---VYGFGSGKRGQLGVSNDRV-KSVNVPKVVSGFEGVEIAGIAANG 219

Query: 255 DQSAALTVDGHLYTWGKGFHGISDACLPLLPSSALKFTKVALGWNHALLLTDCGTVFMLG 434
           D SAA++VDGH+YTWG+GF G  DA +P   +S+L FTKVALGWNHAL ++  G V MLG
Sbjct: 220 DHSAAVSVDGHVYTWGRGFKGFEDARVPQCLNSSLNFTKVALGWNHALAMSGEGEVCMLG 279

Query: 435 GNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSA 614
           GNH G+LS  Q +       +            R   LEKV GLDG K  DIA GAEHS 
Sbjct: 280 GNHLGVLSDLQNISPAKHLPH-----------LREVNLEKVPGLDGTKITDIATGAEHSV 328

Query: 615 LVTENGKIMTWGWGEHGQLGLGNEEDQIAPQIVSLDAKLLKVQTLFKVYCGSGFTFA 785
           +VTE+G+I TWGWGEHGQLGLG+  D+I+P  VSL   L +  ++  V+CGSGFTFA
Sbjct: 329 IVTEHGEIKTWGWGEHGQLGLGDTRDRISPVTVSLGYDLNEAASVI-VFCGSGFTFA 384



 Score = 67.0 bits (162), Expect = 1e-08
 Identities = 56/216 (25%), Positives = 92/216 (42%), Gaps = 11/216 (5%)
 Frame = +3

Query: 102 KGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVD 281
           K  S   ++ +G+G  GQLGT    I +  + PQ++      +I+ +   G    ALT  
Sbjct: 13  KDESEQRVWSWGAGTEGQLGTK---ILQDEHFPQLLHQPSLSSISSLACGGAHVIALTSA 69

Query: 282 GHLYTWGKGFHG-------ISDACLPLLPSSALKF--TKVALGWNHALLLTDCGTVFMLG 434
           G + +WG+G  G       +S++  P   +S   +  T V+ GW H+  ++D G VF  G
Sbjct: 70  GKVLSWGRGNSGQLGHGLVVSNSLYPKAVTSLDGYFITHVSAGWGHSGFVSDNGCVFTCG 129

Query: 435 GNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDCRLSILEKVSGLDGIKAVDIAAGAEHSA 614
               G L                    G  D        KVS         +A G  HS 
Sbjct: 130 DGSFGQL--------------------GHGDHASHCSPVKVSCFVDQHVEQVACGMRHSL 169

Query: 615 LVTENGKIMTWGWGEHGQLGLGNE--EDQIAPQIVS 716
           ++ +  ++  +G G+ GQLG+ N+  +    P++VS
Sbjct: 170 VLLKGNQVYGFGSGKRGQLGVSNDRVKSVNVPKVVS 205