BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00020722
         (1697 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI39149.3| unnamed protein product [Vitis vinifera]              645   0.0  
ref|XP_002267641.1| PREDICTED: 2,3-dimethylmalate lyase [Vitis v...   645   0.0  
ref|XP_002301634.1| predicted protein [Populus trichocarpa] gi|2...   638   e-180
gb|ABN08672.1| Isocitrate lyase and phosphorylmutase [Medicago t...   620   e-175
ref|XP_003621025.1| Isocitrate lyase [Medicago truncatula] gi|35...   612   e-173

>emb|CBI39149.3| unnamed protein product [Vitis vinifera]
          Length = 449

 Score =  645 bits (1665), Expect = 0.0
 Identities = 321/420 (76%), Positives = 369/420 (87%)
 Frame = +1

Query: 328  LRRLLESRGTTHLGPACFDALSAKLIQQAGFDFCFTSGFSLSAAKLGLPDVGLISYGEMV 507
            LRR+L+S G  H GPACFDALSA+L+++AGF +CFTSGFS+SAA+LGLPDVGLISYGEM+
Sbjct: 35   LRRILDSPGV-HQGPACFDALSAQLVERAGFQYCFTSGFSISAARLGLPDVGLISYGEML 93

Query: 508  DQGRLITQAVSIPVIGDGDNGYGNAMNVKRTVKGYIHAGFAGIILEDQVSPKACGHTQGR 687
            DQGR ITQAVSIPVIGDGDNGYGNAMNVKRTVK +I AGFAGIILEDQVSPKACGHT+GR
Sbjct: 94   DQGRQITQAVSIPVIGDGDNGYGNAMNVKRTVKDFIRAGFAGIILEDQVSPKACGHTRGR 153

Query: 688  KVVSREEAVMRIKAAIDARKESGSDIVIVARTDSRQAISFEESLWRSRAFGDAGADVLFI 867
            KVVSREEAVMRIKAAIDARKE+GSDIVIVARTDSRQA+SF+ESLWRSRAF  AGADVLFI
Sbjct: 154  KVVSREEAVMRIKAAIDARKETGSDIVIVARTDSRQAVSFDESLWRSRAFASAGADVLFI 213

Query: 868  DALASREEMEKFCQIFPLLPKMANMLEGGGKSPILHPIELTEMGFRLVSYPLSLIGVSIR 1047
            DAL+SREEM+ FC+I P +PKMANMLEGGGK+PIL+PIEL ++G+++V YPLSLIGVSIR
Sbjct: 214  DALSSREEMKAFCEIAPFVPKMANMLEGGGKTPILNPIELEDVGYKIVVYPLSLIGVSIR 273

Query: 1048 AMQDALKGIKGGRIPPPASLPSFDEIKEILGFNAYYEEEKQYDTSTNQSDYQRGYXXXXX 1227
            AMQDAL+ IKGGRIP P S+P+F+EIKE LGFN+YYEEEK+Y TS  Q  +QR       
Sbjct: 274  AMQDALRAIKGGRIPSPGSMPTFEEIKETLGFNSYYEEEKRYATSKGQVSWQRA----SS 329

Query: 1228 NIYTIQQQTPDDTEQEGQIEQEPIVEVITPDMYYAPDGSKGPLARTSSRTLRVKITGRDG 1407
            N Y+ QQ+  DDT+Q GQ  Q+P+VEV+TPD YY PDGSK P A   SRTLR+KITGRDG
Sbjct: 330  NTYSSQQRAQDDTQQRGQNSQDPVVEVLTPDAYYGPDGSKVPFAGIWSRTLRIKITGRDG 389

Query: 1408 SEKLEIRIPAGFLEGITNVVPALGGVNLRALLDDARDEVGGTKLIDFNDTMGDRIQVFLE 1587
             +KL++RIPAGFLEGITN+VPALGGVN++ LLDDA +E+GG  LIDFND MGDRIQVFLE
Sbjct: 390  IDKLDVRIPAGFLEGITNIVPALGGVNIKELLDDAAEELGGKLLIDFNDAMGDRIQVFLE 449


>ref|XP_002267641.1| PREDICTED: 2,3-dimethylmalate lyase [Vitis vinifera]
          Length = 505

 Score =  645 bits (1665), Expect = 0.0
 Identities = 321/420 (76%), Positives = 369/420 (87%)
 Frame = +1

Query: 328  LRRLLESRGTTHLGPACFDALSAKLIQQAGFDFCFTSGFSLSAAKLGLPDVGLISYGEMV 507
            LRR+L+S G  H GPACFDALSA+L+++AGF +CFTSGFS+SAA+LGLPDVGLISYGEM+
Sbjct: 91   LRRILDSPGV-HQGPACFDALSAQLVERAGFQYCFTSGFSISAARLGLPDVGLISYGEML 149

Query: 508  DQGRLITQAVSIPVIGDGDNGYGNAMNVKRTVKGYIHAGFAGIILEDQVSPKACGHTQGR 687
            DQGR ITQAVSIPVIGDGDNGYGNAMNVKRTVK +I AGFAGIILEDQVSPKACGHT+GR
Sbjct: 150  DQGRQITQAVSIPVIGDGDNGYGNAMNVKRTVKDFIRAGFAGIILEDQVSPKACGHTRGR 209

Query: 688  KVVSREEAVMRIKAAIDARKESGSDIVIVARTDSRQAISFEESLWRSRAFGDAGADVLFI 867
            KVVSREEAVMRIKAAIDARKE+GSDIVIVARTDSRQA+SF+ESLWRSRAF  AGADVLFI
Sbjct: 210  KVVSREEAVMRIKAAIDARKETGSDIVIVARTDSRQAVSFDESLWRSRAFASAGADVLFI 269

Query: 868  DALASREEMEKFCQIFPLLPKMANMLEGGGKSPILHPIELTEMGFRLVSYPLSLIGVSIR 1047
            DAL+SREEM+ FC+I P +PKMANMLEGGGK+PIL+PIEL ++G+++V YPLSLIGVSIR
Sbjct: 270  DALSSREEMKAFCEIAPFVPKMANMLEGGGKTPILNPIELEDVGYKIVVYPLSLIGVSIR 329

Query: 1048 AMQDALKGIKGGRIPPPASLPSFDEIKEILGFNAYYEEEKQYDTSTNQSDYQRGYXXXXX 1227
            AMQDAL+ IKGGRIP P S+P+F+EIKE LGFN+YYEEEK+Y TS  Q  +QR       
Sbjct: 330  AMQDALRAIKGGRIPSPGSMPTFEEIKETLGFNSYYEEEKRYATSKGQVSWQRA----SS 385

Query: 1228 NIYTIQQQTPDDTEQEGQIEQEPIVEVITPDMYYAPDGSKGPLARTSSRTLRVKITGRDG 1407
            N Y+ QQ+  DDT+Q GQ  Q+P+VEV+TPD YY PDGSK P A   SRTLR+KITGRDG
Sbjct: 386  NTYSSQQRAQDDTQQRGQNSQDPVVEVLTPDAYYGPDGSKVPFAGIWSRTLRIKITGRDG 445

Query: 1408 SEKLEIRIPAGFLEGITNVVPALGGVNLRALLDDARDEVGGTKLIDFNDTMGDRIQVFLE 1587
             +KL++RIPAGFLEGITN+VPALGGVN++ LLDDA +E+GG  LIDFND MGDRIQVFLE
Sbjct: 446  IDKLDVRIPAGFLEGITNIVPALGGVNIKELLDDAAEELGGKLLIDFNDAMGDRIQVFLE 505


>ref|XP_002301634.1| predicted protein [Populus trichocarpa] gi|222843360|gb|EEE80907.1|
            predicted protein [Populus trichocarpa]
          Length = 504

 Score =  638 bits (1645), Expect = e-180
 Identities = 327/448 (72%), Positives = 380/448 (84%), Gaps = 2/448 (0%)
 Frame = +1

Query: 250  KPISCSSDQIQKAQNTSLYSSKKVLALRRLLESRGTTHLGPACFDALSAKLIQQAGFDFC 429
            K ++CS+      +N S  ++KK   LR +L+S G  H GPACFDALSA L+Q+AGFD+C
Sbjct: 70   KMVTCSASA---EKNNS--AAKK---LRLILDSPGV-HQGPACFDALSALLVQRAGFDYC 120

Query: 430  FTSGFSLSAAKLGLPDVGLISYGEMVDQGRLITQAVSIPVIGDGDNGYGNAMNVKRTVKG 609
            FTSGFS+SAAKLGLPD G ISYGEMVDQG+ ITQAVSIPVIGD DNGYGN MNVKRTVKG
Sbjct: 121  FTSGFSISAAKLGLPDTGFISYGEMVDQGQQITQAVSIPVIGDADNGYGNPMNVKRTVKG 180

Query: 610  YIHAGFAGIILEDQVSPKACGHTQGRKVVSREEAVMRIKAAIDARKESGSDIVIVARTDS 789
            YI AGFAGIILEDQVSPKACGHT+GRKVVSREEA+MRIKAA+DAR+E+GSDIVIV+RTDS
Sbjct: 181  YIRAGFAGIILEDQVSPKACGHTRGRKVVSREEAIMRIKAAVDAREETGSDIVIVSRTDS 240

Query: 790  RQAISFEESLWRSRAFGDAGADVLFIDALASREEMEKFCQIFPLLPKMANMLEGGGKSPI 969
            RQA+S +ESLWRSRAF DAGADVLFIDALASREEM+ FC+I PL+PKMANMLEGGGK+PI
Sbjct: 241  RQAVSLDESLWRSRAFADAGADVLFIDALASREEMKSFCEISPLVPKMANMLEGGGKTPI 300

Query: 970  LHPIELTEMGFRLVSYPLSLIGVSIRAMQDALKGIKGGRIPPPASLPSFDEIKEILGFNA 1149
            + P EL E+G++LV+YPLSLIGVSIRAMQD+L  IKGGRIPPP S+PSF+EIK+ILGFN 
Sbjct: 301  VTPFELEEVGYKLVAYPLSLIGVSIRAMQDSLAAIKGGRIPPPGSMPSFEEIKDILGFNT 360

Query: 1150 YYEEEKQYDTSTNQSDYQRGYXXXXXNIYTIQQQTPDDTEQEGQIEQEPIVEVITPDMY- 1326
            YYEEEKQY  S++Q   QR       N+Y IQ++TPD TEQ  Q  Q+P+VEVITP++Y 
Sbjct: 361  YYEEEKQYAISSSQMARQRA----SSNVYGIQRRTPDYTEQRSQSPQDPVVEVITPEVYG 416

Query: 1327 -YAPDGSKGPLARTSSRTLRVKITGRDGSEKLEIRIPAGFLEGITNVVPALGGVNLRALL 1503
             Y  D S+GP +   SRTLRVKITGRDG EKL++RIPAGFLEGITN+VPALGGVN++ LL
Sbjct: 417  GYGADNSRGPFSGIWSRTLRVKITGRDGFEKLDVRIPAGFLEGITNIVPALGGVNIKGLL 476

Query: 1504 DDARDEVGGTKLIDFNDTMGDRIQVFLE 1587
            DDA +EVGG  L+DFNDT+GDRIQVFLE
Sbjct: 477  DDAAEEVGGKLLLDFNDTVGDRIQVFLE 504


>gb|ABN08672.1| Isocitrate lyase and phosphorylmutase [Medicago truncatula]
          Length = 478

 Score =  620 bits (1598), Expect = e-175
 Identities = 316/437 (72%), Positives = 369/437 (84%), Gaps = 2/437 (0%)
 Frame = +1

Query: 283  KAQNTSLYSSKKVLALRRLLESRGTTHLGPACFDALSAKLIQQAGFDFCFTSGFSLSAAK 462
            +A  T   SS K   LR +L+S G  H GPACFDALSA L+Q AGF  CFTSGFS+SA++
Sbjct: 48   RATITISCSSNKAKQLREILDSPGV-HQGPACFDALSANLVQSAGFPLCFTSGFSISASR 106

Query: 463  LGLPDVGLISYGEMVDQGRLITQAVSIPVIGDGDNGYGNAMNVKRTVKGYIHAGFAGIIL 642
            LGLPD G +SYGE+ DQG LITQ+V IPVIGD DNGYGNAMNVKRTVKGY++AGFAGIIL
Sbjct: 107  LGLPDTGYLSYGEIFDQGLLITQSVDIPVIGDADNGYGNAMNVKRTVKGYVNAGFAGIIL 166

Query: 643  EDQVSPKACGHTQGRKVVSREEAVMRIKAAIDARKESGSDIVIVARTDSRQAISFEESLW 822
            EDQVSPKACGHTQGRKVVSREEAVMRIKAA+DAR ESGSDIVIVARTD+RQA+S +E+L+
Sbjct: 167  EDQVSPKACGHTQGRKVVSREEAVMRIKAAVDARNESGSDIVIVARTDARQALSLDEALY 226

Query: 823  RSRAFGDAGADVLFIDALASREEMEKFCQIFPLLPKMANMLEGGGKSPILHPIELTEMGF 1002
            RSRAF DAGADV+FIDALASR+EME FCQ+ PL+PKMANMLEGGGK+PIL P+EL ++G+
Sbjct: 227  RSRAFADAGADVVFIDALASRQEMEAFCQVSPLVPKMANMLEGGGKTPILTPLELEDIGY 286

Query: 1003 RLVSYPLSLIGVSIRAMQDALKGIKGGRIPPPASLPSFDEIKEILGFNAYYEEEKQYDTS 1182
            ++V+YPLSLIGVSIRAMQDAL  IKGGRIPPP S+P+F+EIK+ LGFNAYYEEEK+Y TS
Sbjct: 287  KIVAYPLSLIGVSIRAMQDALTAIKGGRIPPPGSMPTFEEIKDTLGFNAYYEEEKRYATS 346

Query: 1183 TNQSDYQRGYXXXXXNIYTIQQQTPDDTEQEGQIEQEPIVEVITPDMY--YAPDGSKGPL 1356
            T+Q   +R       ++Y+IQQ+   DTEQ  Q  ++PIVEVITPD+Y  Y  DGS+ P 
Sbjct: 347  TDQLLSKR----ESSSVYSIQQREQVDTEQTSQTIEDPIVEVITPDVYNKYGADGSRDPF 402

Query: 1357 ARTSSRTLRVKITGRDGSEKLEIRIPAGFLEGITNVVPALGGVNLRALLDDARDEVGGTK 1536
            +   SRTLR+KITGRDG EKL++RIPAGFL+GITN VPALGGVNL+ LLDDA DE+GG K
Sbjct: 403  SGIWSRTLRIKITGRDGVEKLDLRIPAGFLDGITNTVPALGGVNLKKLLDDATDEIGG-K 461

Query: 1537 LIDFNDTMGDRIQVFLE 1587
            L+DFND MGDRIQVFLE
Sbjct: 462  LLDFNDRMGDRIQVFLE 478


>ref|XP_003621025.1| Isocitrate lyase [Medicago truncatula] gi|355496040|gb|AES77243.1|
            Isocitrate lyase [Medicago truncatula]
          Length = 467

 Score =  612 bits (1579), Expect = e-173
 Identities = 314/437 (71%), Positives = 363/437 (83%), Gaps = 2/437 (0%)
 Frame = +1

Query: 283  KAQNTSLYSSKKVLALRRLLESRGTTHLGPACFDALSAKLIQQAGFDFCFTSGFSLSAAK 462
            +A  T   SS K   LR +L+S G  H GPACFDALSA L+Q AGF  CFTSGFS+SA++
Sbjct: 48   RATITISCSSNKAKQLREILDSPGV-HQGPACFDALSANLVQSAGFPLCFTSGFSISASR 106

Query: 463  LGLPDVGLISYGEMVDQGRLITQAVSIPVIGDGDNGYGNAMNVKRTVKGYIHAGFAGIIL 642
            LGLPD G +SYGE+ DQG LITQ+V IPVIGD DNGYGNAMNVKRTVKGY++AGFAGIIL
Sbjct: 107  LGLPDTGYLSYGEIFDQGLLITQSVDIPVIGDADNGYGNAMNVKRTVKGYVNAGFAGIIL 166

Query: 643  EDQVSPKACGHTQGRKVVSREEAVMRIKAAIDARKESGSDIVIVARTDSRQAISFEESLW 822
            EDQVSPKACGHTQGRKVVSREEAVMRIKAA+DAR ESGSDIVIVARTD+RQA+S +E+L+
Sbjct: 167  EDQVSPKACGHTQGRKVVSREEAVMRIKAAVDARNESGSDIVIVARTDARQALSLDEALY 226

Query: 823  RSRAFGDAGADVLFIDALASREEMEKFCQIFPLLPKMANMLEGGGKSPILHPIELTEMGF 1002
            RSRAF DAGADV+FIDALASR+EME FCQ+ PL+PKMANMLEGGGK+PIL P+EL ++G+
Sbjct: 227  RSRAFADAGADVVFIDALASRQEMEAFCQVSPLVPKMANMLEGGGKTPILTPLELEDIGY 286

Query: 1003 RLVSYPLSLIGVSIRAMQDALKGIKGGRIPPPASLPSFDEIKEILGFNAYYEEEKQYDTS 1182
            ++V+YPLSLIGVSIRAMQDAL  IKGGRIPPP S+P+F+EIK+ LGFNAYYEEEK+Y TS
Sbjct: 287  KIVAYPLSLIGVSIRAMQDALTAIKGGRIPPPGSMPTFEEIKDTLGFNAYYEEEKRYATS 346

Query: 1183 TNQSDYQRGYXXXXXNIYTIQQQTPDDTEQEGQIEQEPIVEVITPDMY--YAPDGSKGPL 1356
            T+Q                IQQ+   DTEQ  Q  ++PIVEVITPD+Y  Y  DGS+ P 
Sbjct: 347  TDQ---------------LIQQREQVDTEQTSQTIEDPIVEVITPDVYNKYGADGSRDPF 391

Query: 1357 ARTSSRTLRVKITGRDGSEKLEIRIPAGFLEGITNVVPALGGVNLRALLDDARDEVGGTK 1536
            +   SRTLR+KITGRDG EKL++RIPAGFL+GITN VPALGGVNL+ LLDDA DE+GG K
Sbjct: 392  SGIWSRTLRIKITGRDGVEKLDLRIPAGFLDGITNTVPALGGVNLKKLLDDATDEIGG-K 450

Query: 1537 LIDFNDTMGDRIQVFLE 1587
            L+DFND MGDRIQVFLE
Sbjct: 451  LLDFNDRMGDRIQVFLE 467