BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00019834
         (1141 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510600.1| s-adenosyl-l-methionine:delta24-sterol-C-met...   621   e-176
ref|NP_173458.1| 24-methylenesterol C-methyltransferase 2 [Arabi...   618   e-174
ref|XP_002893098.1| hypothetical protein ARALYDRAFT_472261 [Arab...   617   e-174
gb|AAM91592.1| 24-sterol C-methyltransferase [Arabidopsis thalia...   617   e-174
gb|AAN31890.1| putative sterol-C-methyltransferase [Arabidopsis ...   615   e-174

>ref|XP_002510600.1| s-adenosyl-l-methionine:delta24-sterol-C-methyltransferase, putative
            [Ricinus communis] gi|223551301|gb|EEF52787.1|
            s-adenosyl-l-methionine:delta24-sterol-C-
            methyltransferase, putative [Ricinus communis]
          Length = 361

 Score =  621 bits (1602), Expect = e-176
 Identities = 294/355 (82%), Positives = 323/355 (90%)
 Frame = +2

Query: 56   MDSFSLFFTTALLAGGLYWFVFFFGSAERKGKRAVSLSGGSISRDNLQDNYKQYWSFFRR 235
            MDSFSLF T ALLA GLYWFV   G AE KGKRAV LSGGSIS + +QDNYKQYWSFFRR
Sbjct: 1    MDSFSLFCTGALLAVGLYWFVCILGPAEVKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 236  PKEIETVEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNKDATRFHEEMAVDLLD 415
            PKEIET EKVP FVDTFYNLVTDIYEWGWGQSFHFSPS+PGKS++DATR HEEMAVDL+D
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSVPGKSHRDATRLHEEMAVDLID 120

Query: 416  VNHGQKILDVGCGVGGPMRAIAAHSKAHVVGITINEYQVKRALIHNKKAGLDSLCEVVCG 595
            V  G +ILDVGCGVGGPMRAIAAHS+A+VVGITINEYQV RA +HNKKAGLDSLCEVVCG
Sbjct: 121  VKPGDRILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARLHNKKAGLDSLCEVVCG 180

Query: 596  NFLEMPFDDNSFDGAYSIEATCHAPKLEQVYAEIYRVLKPGSKYVSYEWVTTDRYDGNNE 775
            NFLEMPF DNSFDGAYSIEATCHAPKLE+VYAEI+RVLKPGS YVSYEWVTTD+Y  ++ 
Sbjct: 181  NFLEMPFPDNSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYRASDS 240

Query: 776  EHVEVIQGIERGNALPGLRRYDEISEIAKKVGFEVVKEKDLAKPPSHPWWDRLKMGRIAY 955
            EHVE+IQGIERG+ALPGLR Y +I+E A+KVGFEVVKEKDLAKPP+ PWW RLKMGRIAY
Sbjct: 241  EHVEIIQGIERGDALPGLRNYIDIAETARKVGFEVVKEKDLAKPPAQPWWTRLKMGRIAY 300

Query: 956  WRNHVLVTVLTWLGIAPKGVVDVHEMLFVTADYLTRGGESGIFSPMHLIVCRKPE 1120
            WRNH+LVT+L+ +GIAPKG VDVHEMLF TADYLTRGGE+GIF+PMH+I+CRKPE
Sbjct: 301  WRNHILVTMLSAIGIAPKGTVDVHEMLFKTADYLTRGGETGIFTPMHMILCRKPE 355


>ref|NP_173458.1| 24-methylenesterol C-methyltransferase 2 [Arabidopsis thaliana]
            gi|62900893|sp|Q39227.2|SMT2_ARATH RecName:
            Full=24-methylenesterol C-methyltransferase 2;
            Short=24-sterol C-methyltransferase 2;
            Short=Sterol-C-methyltransferase 2; AltName: Full=Protein
            COTYLEDON VASCULAR PATTERN 1
            gi|9558593|gb|AAF88156.1|AC026234_7 Identical to
            24-sterol C-methyltransferase from Arabidopsis thaliana
            gi|2129517 and is a member of the ubiE/COQ5
            methyltransferase family PF|01209. ESTs gb|T42228,
            gb|T46520, gb|T41746, gb|N38458, gb|AI993515,
            gb|AA389843, gb|AI099890, gb|AI099653 come from this gene
            [Arabidopsis thaliana]
            gi|12083242|gb|AAG48780.1|AF332417_1 putative
            sterol-C-methyltransferase [Arabidopsis thaliana]
            gi|15028255|gb|AAK76716.1| putative
            sterol-C-methyltransferase [Arabidopsis thaliana]
            gi|21281107|gb|AAM45009.1| putative
            sterol-C-methyltransferase [Arabidopsis thaliana]
            gi|332191841|gb|AEE29962.1| 24-methylenesterol
            C-methyltransferase 2 [Arabidopsis thaliana]
          Length = 361

 Score =  618 bits (1593), Expect = e-174
 Identities = 288/356 (80%), Positives = 324/356 (91%)
 Frame = +2

Query: 56   MDSFSLFFTTALLAGGLYWFVFFFGSAERKGKRAVSLSGGSISRDNLQDNYKQYWSFFRR 235
            MDS +LFFT AL+A G+YWF+   G AERKGKRAV LSGGSIS + +QDNYKQYWSFFRR
Sbjct: 1    MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 236  PKEIETVEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNKDATRFHEEMAVDLLD 415
            PKEIET EKVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+KDATR HEEMAVDL+ 
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 416  VNHGQKILDVGCGVGGPMRAIAAHSKAHVVGITINEYQVKRALIHNKKAGLDSLCEVVCG 595
            V  GQKILDVGCGVGGPMRAIA+HS+A+VVGITINEYQV RA +HNKKAGLD+LCEVVCG
Sbjct: 121  VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 596  NFLEMPFDDNSFDGAYSIEATCHAPKLEQVYAEIYRVLKPGSKYVSYEWVTTDRYDGNNE 775
            NFL+MPFDDNSFDGAYSIEATCHAPKLE+VYAEIYRVLKPGS YVSYEWVTT+++   ++
Sbjct: 181  NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 776  EHVEVIQGIERGNALPGLRRYDEISEIAKKVGFEVVKEKDLAKPPSHPWWDRLKMGRIAY 955
            EHVEVIQGIERG+ALPGLR Y +I+E AKKVGFE+VKEKDLA PP+ PWW RLKMGR+AY
Sbjct: 241  EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 956  WRNHVLVTVLTWLGIAPKGVVDVHEMLFVTADYLTRGGESGIFSPMHLIVCRKPEA 1123
            WRNH++V +L+ +G+APKG VDVHEMLF TADYLTRGGE+GIFSPMH+I+CRKPE+
Sbjct: 301  WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPES 356


>ref|XP_002893098.1| hypothetical protein ARALYDRAFT_472261 [Arabidopsis lyrata subsp.
            lyrata] gi|297338940|gb|EFH69357.1| hypothetical protein
            ARALYDRAFT_472261 [Arabidopsis lyrata subsp. lyrata]
          Length = 365

 Score =  617 bits (1592), Expect = e-174
 Identities = 288/356 (80%), Positives = 324/356 (91%)
 Frame = +2

Query: 56   MDSFSLFFTTALLAGGLYWFVFFFGSAERKGKRAVSLSGGSISRDNLQDNYKQYWSFFRR 235
            MDS +LFFT AL+A G+YWF+   G AERKGKRAV LSGGSIS + +QDNYKQYWSFFRR
Sbjct: 1    MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 236  PKEIETVEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNKDATRFHEEMAVDLLD 415
            PKEIET EKVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+KDATR HEEMAVDL+ 
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 416  VNHGQKILDVGCGVGGPMRAIAAHSKAHVVGITINEYQVKRALIHNKKAGLDSLCEVVCG 595
            V  GQKILDVGCGVGGPMRAIA+HS+A+VVGITINEYQV RA +HNKKAGLD+LCEVVCG
Sbjct: 121  VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 596  NFLEMPFDDNSFDGAYSIEATCHAPKLEQVYAEIYRVLKPGSKYVSYEWVTTDRYDGNNE 775
            NFL+MPFDDNSFDGAYSIEATCHAPKLE+VYAEIYRVLKPGS YVSYEWVTT+++   ++
Sbjct: 181  NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 776  EHVEVIQGIERGNALPGLRRYDEISEIAKKVGFEVVKEKDLAKPPSHPWWDRLKMGRIAY 955
            EHVEVIQGIERG+ALPGLR Y +I+E AKKVGFE+VKEKDLA PP+ PWW RLKMGR+AY
Sbjct: 241  EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAKPWWTRLKMGRLAY 300

Query: 956  WRNHVLVTVLTWLGIAPKGVVDVHEMLFVTADYLTRGGESGIFSPMHLIVCRKPEA 1123
            WRNH++V +L+ +G+APKG VDVHEMLF TADYLTRGGE+GIFSPMH+I+CRKPE+
Sbjct: 301  WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPES 356


>gb|AAM91592.1| 24-sterol C-methyltransferase [Arabidopsis thaliana]
            gi|25083682|gb|AAN72104.1| 24-sterol C-methyltransferase
            [Arabidopsis thaliana]
          Length = 361

 Score =  617 bits (1590), Expect = e-174
 Identities = 287/356 (80%), Positives = 324/356 (91%)
 Frame = +2

Query: 56   MDSFSLFFTTALLAGGLYWFVFFFGSAERKGKRAVSLSGGSISRDNLQDNYKQYWSFFRR 235
            MDS +LFFT AL+A G+YWF+   G AERKGKRAV LSGGSIS + +QDNYKQYWSFFRR
Sbjct: 1    MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 236  PKEIETVEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNKDATRFHEEMAVDLLD 415
            PKEIET EKVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+KDATR HEEMAVDL+ 
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 416  VNHGQKILDVGCGVGGPMRAIAAHSKAHVVGITINEYQVKRALIHNKKAGLDSLCEVVCG 595
            V  GQKILDVGCGVGGPMRAIA+HS+A+VVGITINEYQV RA +HNKKAGLD+LCEVVCG
Sbjct: 121  VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 596  NFLEMPFDDNSFDGAYSIEATCHAPKLEQVYAEIYRVLKPGSKYVSYEWVTTDRYDGNNE 775
            NFL+MPFDDNSFDGAYSIEATCHAPKLE+VYAEIYRVLKPGS YVSYEWVTT+++   ++
Sbjct: 181  NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 776  EHVEVIQGIERGNALPGLRRYDEISEIAKKVGFEVVKEKDLAKPPSHPWWDRLKMGRIAY 955
            EHVEVIQGIERG+ALPGLR Y +I+E AKKVGFE+VKEKDLA PP+ PWW RLKMGR+AY
Sbjct: 241  EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 956  WRNHVLVTVLTWLGIAPKGVVDVHEMLFVTADYLTRGGESGIFSPMHLIVCRKPEA 1123
            WRNH++V +L+ +G+APKG VDVHEMLF TADYLTRGG++GIFSPMH+I+CRKPE+
Sbjct: 301  WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGQTGIFSPMHMILCRKPES 356


>gb|AAN31890.1| putative sterol-C-methyltransferase [Arabidopsis thaliana]
          Length = 361

 Score =  615 bits (1585), Expect = e-174
 Identities = 287/356 (80%), Positives = 323/356 (90%)
 Frame = +2

Query: 56   MDSFSLFFTTALLAGGLYWFVFFFGSAERKGKRAVSLSGGSISRDNLQDNYKQYWSFFRR 235
            MDS +LFFT AL+A G+YWF+   G AERKGKRAV LSGGSIS + +QDNYKQYWSFFRR
Sbjct: 1    MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 236  PKEIETVEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNKDATRFHEEMAVDLLD 415
            PKEIET EKVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+KDATR HEEMAVDL+ 
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120

Query: 416  VNHGQKILDVGCGVGGPMRAIAAHSKAHVVGITINEYQVKRALIHNKKAGLDSLCEVVCG 595
            V  GQKILDVGCGVGGPMRAIA+HS+A+VVGITINEYQV RA +HNKKAGLD+LCEVVCG
Sbjct: 121  VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180

Query: 596  NFLEMPFDDNSFDGAYSIEATCHAPKLEQVYAEIYRVLKPGSKYVSYEWVTTDRYDGNNE 775
            NFL+MPFDDNSFDGAYSIEATCHAPKLE+VYAEIYRVLKPGS YVSYEWVTT+++   ++
Sbjct: 181  NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240

Query: 776  EHVEVIQGIERGNALPGLRRYDEISEIAKKVGFEVVKEKDLAKPPSHPWWDRLKMGRIAY 955
            EHVEVIQGIERG+ALPGLR Y +I+E AKKVGFE+VKEKDLA PP+ PWW RLKMGR+AY
Sbjct: 241  EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300

Query: 956  WRNHVLVTVLTWLGIAPKGVVDVHEMLFVTADYLTRGGESGIFSPMHLIVCRKPEA 1123
            WRNH++V +L+ +G+APKG VDVHEMLF TADYLTRGGE+GIFSPMH+I+CRK E+
Sbjct: 301  WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKAES 356