BLASTX nr result
ID: Coptis25_contig00019386
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00019386
(946 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004135943.1| PREDICTED: alpha-N-acetylglucosaminidase-lik... 149 2e-44
ref|XP_004158231.1| PREDICTED: LOW QUALITY PROTEIN: alpha-N-acet... 149 2e-44
emb|CBI24942.3| unnamed protein product [Vitis vinifera] 174 3e-41
ref|XP_002273084.1| PREDICTED: alpha-N-acetylglucosaminidase-lik... 174 3e-41
ref|XP_002314048.1| predicted protein [Populus trichocarpa] gi|2... 171 2e-40
>ref|XP_004135943.1| PREDICTED: alpha-N-acetylglucosaminidase-like [Cucumis sativus]
Length = 774
Score = 149 bits (376), Expect(3) = 2e-44
Identities = 65/84 (77%), Positives = 76/84 (90%)
Frame = +1
Query: 127 YIQLGWLFTNEFEFWKPPQMRALLHSVPFGKMIVLDLSAEVRPIWEKSSQFYGTPYVWCI 306
++ GWLF ++ +FWKP QM+ALLHSVPFGKMIVLDL A+V+PIW+ SSQFYGTPYVWC+
Sbjct: 375 WLMQGWLFYSDSDFWKPDQMKALLHSVPFGKMIVLDLFADVKPIWKSSSQFYGTPYVWCM 434
Query: 307 LHNFGGNIEMYGILDVISSGPVEA 378
LHNFGGNIEMYGILD ISSGPV+A
Sbjct: 435 LHNFGGNIEMYGILDAISSGPVDA 458
Score = 55.5 bits (132), Expect(3) = 2e-44
Identities = 27/35 (77%), Positives = 29/35 (82%)
Frame = +1
Query: 1 MIELGMTPVLPSFSGNVPAAIKIAFPSANITRLKN 105
M ELGMTPVLPSFSGNVPA + FPSANIT+L N
Sbjct: 258 MRELGMTPVLPSFSGNVPAGLVEIFPSANITKLGN 292
Score = 21.9 bits (45), Expect(3) = 2e-44
Identities = 7/10 (70%), Positives = 10/10 (100%)
Frame = +2
Query: 107 EYGDITDIYN 136
EYGD+T+IY+
Sbjct: 329 EYGDVTNIYS 338
Score = 136 bits (342), Expect = 8e-30
Identities = 72/145 (49%), Positives = 88/145 (60%)
Frame = +3
Query: 510 EQNPVVFELMSEMAFRSEKVQLEEWLKSYSYRRYGKKINLIEDAWEILHRTIYNCTDRIS 689
E NPVV+ELMSEMAFRS+KVQ++EWLK+YS RYGK + ++ AW IL+ TIYNCTD I+
Sbjct: 477 EHNPVVYELMSEMAFRSKKVQVQEWLKTYSRCRYGKADHYVDAAWNILYHTIYNCTDGIA 536
Query: 690 DHNKDFIVKFPDFDPSSYFQSNMSEDSHIGNLFSQGTTNRFSSRETTFVLPQHNQPHLWY 869
+HN DFIVK PD+DPSS F PHLWY
Sbjct: 537 NHNTDFIVKLPDWDPSSTFDLK-------------------------------KPPHLWY 565
Query: 870 STEEVIVALQLFLDARNYLAGSLTY 944
ST+EVI ALQL ++ + L S TY
Sbjct: 566 STQEVINALQLLVNVDDNLVHSATY 590
>ref|XP_004158231.1| PREDICTED: LOW QUALITY PROTEIN: alpha-N-acetylglucosaminidase-like
[Cucumis sativus]
Length = 567
Score = 149 bits (376), Expect(3) = 2e-44
Identities = 65/84 (77%), Positives = 76/84 (90%)
Frame = +1
Query: 127 YIQLGWLFTNEFEFWKPPQMRALLHSVPFGKMIVLDLSAEVRPIWEKSSQFYGTPYVWCI 306
++ GWLF ++ +FWKP QM+ALLHSVPFGKMIVLDL A+V+PIW+ SSQFYGTPYVWC+
Sbjct: 168 WLMQGWLFYSDSDFWKPDQMKALLHSVPFGKMIVLDLFADVKPIWKSSSQFYGTPYVWCM 227
Query: 307 LHNFGGNIEMYGILDVISSGPVEA 378
LHNFGGNIEMYGILD ISSGPV+A
Sbjct: 228 LHNFGGNIEMYGILDAISSGPVDA 251
Score = 55.5 bits (132), Expect(3) = 2e-44
Identities = 27/35 (77%), Positives = 29/35 (82%)
Frame = +1
Query: 1 MIELGMTPVLPSFSGNVPAAIKIAFPSANITRLKN 105
M ELGMTPVLPSFSGNVPA + FPSANIT+L N
Sbjct: 51 MRELGMTPVLPSFSGNVPAGLVEIFPSANITKLGN 85
Score = 21.9 bits (45), Expect(3) = 2e-44
Identities = 7/10 (70%), Positives = 10/10 (100%)
Frame = +2
Query: 107 EYGDITDIYN 136
EYGD+T+IY+
Sbjct: 122 EYGDVTNIYS 131
Score = 134 bits (338), Expect = 2e-29
Identities = 71/145 (48%), Positives = 87/145 (60%)
Frame = +3
Query: 510 EQNPVVFELMSEMAFRSEKVQLEEWLKSYSYRRYGKKINLIEDAWEILHRTIYNCTDRIS 689
E NPVV+ELMSEMAFR +KVQ++EWLK+YS RYGK + ++ AW IL+ TIYNCTD I+
Sbjct: 270 EHNPVVYELMSEMAFRXQKVQVQEWLKTYSRCRYGKADHYVDAAWNILYHTIYNCTDGIA 329
Query: 690 DHNKDFIVKFPDFDPSSYFQSNMSEDSHIGNLFSQGTTNRFSSRETTFVLPQHNQPHLWY 869
+HN DFIVK PD+DPSS F PHLWY
Sbjct: 330 NHNTDFIVKLPDWDPSSTFDLK-------------------------------KPPHLWY 358
Query: 870 STEEVIVALQLFLDARNYLAGSLTY 944
ST+EVI ALQL ++ + L S TY
Sbjct: 359 STQEVINALQLLVNVDDNLVHSATY 383
>emb|CBI24942.3| unnamed protein product [Vitis vinifera]
Length = 868
Score = 174 bits (441), Expect = 3e-41
Identities = 89/146 (60%), Positives = 110/146 (75%), Gaps = 1/146 (0%)
Frame = +3
Query: 510 EQNPVVFELMSEMAFRSEKVQLEEWLKSYSYRRYGKKINLIEDAWEILHRTIYNCTDRIS 689
EQNPV +ELMSEMAFRSEKVQL EWLK+YSYRRYGK ++ +E AWEIL+RTIYNCTD I+
Sbjct: 542 EQNPVAYELMSEMAFRSEKVQLVEWLKTYSYRRYGKAVHHVEAAWEILYRTIYNCTDGIA 601
Query: 690 DHNKDFIVKFPDFDPSSYFQSNMSEDSH-IGNLFSQGTTNRFSSRETTFVLPQHNQPHLW 866
DHN DF+V FPD+DPS S++S++ H I + +Q + +ET+ LP Q HLW
Sbjct: 602 DHNTDFMVNFPDWDPSLNPSSDISKEQHIIQKILTQTGRRKILFQETSSDLP---QSHLW 658
Query: 867 YSTEEVIVALQLFLDARNYLAGSLTY 944
YST EV+ AL+LFLDA N L+ S TY
Sbjct: 659 YSTHEVVNALRLFLDAGNELSKSSTY 684
Score = 148 bits (374), Expect = 2e-33
Identities = 69/116 (59%), Positives = 87/116 (75%)
Frame = +1
Query: 31 PSFSGNVPAAIKIAFPSANITRLKN*IWRYN*YIQLGWLFTNEFEFWKPPQMRALLHSVP 210
P++ ++ AAI A + K+ +W + GWLF ++ FWKPPQM+ALLHSVP
Sbjct: 417 PAYISSLGAAIYKAMSQGD----KDSVW-----LMQGWLFYSDSGFWKPPQMKALLHSVP 467
Query: 211 FGKMIVLDLSAEVRPIWEKSSQFYGTPYVWCILHNFGGNIEMYGILDVISSGPVEA 378
FGKM+VLDL A+ +PIW SSQFYGTPY+WC+LHNFGGNIEMYGILD +SSGPV+A
Sbjct: 468 FGKMVVLDLFADAKPIWRTSSQFYGTPYIWCMLHNFGGNIEMYGILDAVSSGPVDA 523
Score = 59.3 bits (142), Expect = 1e-06
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +1
Query: 1 MIELGMTPVLPSFSGNVPAAIKIAFPSANITRL 99
M+ELGMTPVLPSFSGNVP A+K FPSANITRL
Sbjct: 323 MLELGMTPVLPSFSGNVPEALKKIFPSANITRL 355
>ref|XP_002273084.1| PREDICTED: alpha-N-acetylglucosaminidase-like [Vitis vinifera]
Length = 803
Score = 174 bits (441), Expect = 3e-41
Identities = 89/146 (60%), Positives = 110/146 (75%), Gaps = 1/146 (0%)
Frame = +3
Query: 510 EQNPVVFELMSEMAFRSEKVQLEEWLKSYSYRRYGKKINLIEDAWEILHRTIYNCTDRIS 689
EQNPV +ELMSEMAFRSEKVQL EWLK+YSYRRYGK ++ +E AWEIL+RTIYNCTD I+
Sbjct: 477 EQNPVAYELMSEMAFRSEKVQLVEWLKTYSYRRYGKAVHHVEAAWEILYRTIYNCTDGIA 536
Query: 690 DHNKDFIVKFPDFDPSSYFQSNMSEDSH-IGNLFSQGTTNRFSSRETTFVLPQHNQPHLW 866
DHN DF+V FPD+DPS S++S++ H I + +Q + +ET+ LP Q HLW
Sbjct: 537 DHNTDFMVNFPDWDPSLNPSSDISKEQHIIQKILTQTGRRKILFQETSSDLP---QSHLW 593
Query: 867 YSTEEVIVALQLFLDARNYLAGSLTY 944
YST EV+ AL+LFLDA N L+ S TY
Sbjct: 594 YSTHEVVNALRLFLDAGNELSKSSTY 619
Score = 148 bits (374), Expect = 2e-33
Identities = 69/116 (59%), Positives = 87/116 (75%)
Frame = +1
Query: 31 PSFSGNVPAAIKIAFPSANITRLKN*IWRYN*YIQLGWLFTNEFEFWKPPQMRALLHSVP 210
P++ ++ AAI A + K+ +W + GWLF ++ FWKPPQM+ALLHSVP
Sbjct: 352 PAYISSLGAAIYKAMSQGD----KDSVW-----LMQGWLFYSDSGFWKPPQMKALLHSVP 402
Query: 211 FGKMIVLDLSAEVRPIWEKSSQFYGTPYVWCILHNFGGNIEMYGILDVISSGPVEA 378
FGKM+VLDL A+ +PIW SSQFYGTPY+WC+LHNFGGNIEMYGILD +SSGPV+A
Sbjct: 403 FGKMVVLDLFADAKPIWRTSSQFYGTPYIWCMLHNFGGNIEMYGILDAVSSGPVDA 458
Score = 59.3 bits (142), Expect = 1e-06
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +1
Query: 1 MIELGMTPVLPSFSGNVPAAIKIAFPSANITRL 99
M+ELGMTPVLPSFSGNVP A+K FPSANITRL
Sbjct: 258 MLELGMTPVLPSFSGNVPEALKKIFPSANITRL 290
>ref|XP_002314048.1| predicted protein [Populus trichocarpa] gi|222850456|gb|EEE88003.1|
predicted protein [Populus trichocarpa]
Length = 806
Score = 171 bits (434), Expect = 2e-40
Identities = 88/145 (60%), Positives = 106/145 (73%)
Frame = +3
Query: 510 EQNPVVFELMSEMAFRSEKVQLEEWLKSYSYRRYGKKINLIEDAWEILHRTIYNCTDRIS 689
E NPVV+ELMSEMAFRS K Q+ EWLK+YS RRYGK + + AW+IL+ TIYNCTD I+
Sbjct: 481 EHNPVVYELMSEMAFRSGKPQVLEWLKTYSRRRYGKAVRQVVAAWDILYHTIYNCTDGIA 540
Query: 690 DHNKDFIVKFPDFDPSSYFQSNMSEDSHIGNLFSQGTTNRFSSRETTFVLPQHNQPHLWY 869
DHN DFIVKFPD+DPS + SN+SE ++ L + T RF +ET+ P + HLWY
Sbjct: 541 DHNTDFIVKFPDWDPSLHSGSNISEQDNMRILLTSSGTRRFLFQETSSDFP---EAHLWY 597
Query: 870 STEEVIVALQLFLDARNYLAGSLTY 944
ST+EVI AL LFLDA N LAGS TY
Sbjct: 598 STQEVIQALWLFLDAGNDLAGSPTY 622
Score = 151 bits (381), Expect = 2e-34
Identities = 72/116 (62%), Positives = 88/116 (75%)
Frame = +1
Query: 31 PSFSGNVPAAIKIAFPSANITRLKN*IWRYN*YIQLGWLFTNEFEFWKPPQMRALLHSVP 210
P++ ++ AA+ A + K+ +W + GWLF ++ FWKPPQM+ALLHSVP
Sbjct: 356 PAYISSLGAAVYKAMSRGD----KDAVW-----LMQGWLFYSDSAFWKPPQMQALLHSVP 406
Query: 211 FGKMIVLDLSAEVRPIWEKSSQFYGTPYVWCILHNFGGNIEMYGILDVISSGPVEA 378
FGKMIVLDL AE +PIW+ SSQFYGTPYVWC+LHNFGGNIEMYGILD ISSGPV+A
Sbjct: 407 FGKMIVLDLFAEAKPIWKNSSQFYGTPYVWCLLHNFGGNIEMYGILDAISSGPVDA 462
Score = 61.2 bits (147), Expect(2) = 4e-09
Identities = 29/33 (87%), Positives = 31/33 (93%)
Frame = +1
Query: 1 MIELGMTPVLPSFSGNVPAAIKIAFPSANITRL 99
M+ELGMTPVLPSFSGNVPAA+K FPSANITRL
Sbjct: 262 MLELGMTPVLPSFSGNVPAALKKIFPSANITRL 294
Score = 26.2 bits (56), Expect(2) = 4e-09
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +2
Query: 107 EYGDITDIYNWVGYSQMS 160
EYGD+TDIYN +++ S
Sbjct: 333 EYGDVTDIYNCDTFNENS 350