BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00018925
         (1632 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       814   0.0  
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   664   0.0  
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   657   0.0  
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       649   0.0  
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh...   551   e-154

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  814 bits (2102), Expect = 0.0
 Identities = 411/460 (89%), Positives = 419/460 (91%)
 Frame = +2

Query: 251  MEGSFLWLIGSXXXXXXXXXXXXRMXXXXXXXXXXWPSGPKRLPIIGNXXXXXXXXXXVT 430
            MEGSFLWLIGS            RM          WPSGPKRLPIIGN          VT
Sbjct: 1    MEGSFLWLIGSTVAIVLVITTIVRMSSSSSSSKKKWPSGPKRLPIIGNLHLLGGDLLHVT 60

Query: 431  LAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEFGQRDTPEIFKIFTVGQNN 610
            LAKLAKVHGSVMTIWIG WRPIIVISDINSAWEVLVSKSAE+GQRDTP+IFK  TVGQNN
Sbjct: 61   LAKLAKVHGSVMTIWIGRWRPIIVISDINSAWEVLVSKSAEYGQRDTPDIFKTLTVGQNN 120

Query: 611  IAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFREEDMKRLIHEMSREALNKNGTIKPLD 790
            I+MSDIGPFWHNVRK LQNGALSPLNVAAQTQFREED+KRLIHEMSREALNKNG IKPLD
Sbjct: 121  ISMSDIGPFWHNVRKGLQNGALSPLNVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLD 180

Query: 791  HMKKNTARLLGRLIFGPTFDNDNFVESMLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKV 970
            HMKKNTARLLGRLIFGPTFDND FVES+LYEIEDVLRLSGYARLTEAFYYAKYLPSQKKV
Sbjct: 181  HMKKNTARLLGRLIFGPTFDNDKFVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKV 240

Query: 971  AREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSST 1150
            AREAYLAKYRIEEL+RPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELF LGVDSTSST
Sbjct: 241  AREAYLAKYRIEELMRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFLLGVDSTSST 300

Query: 1151 ATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMKPIAPLA 1330
            ATWALAYLIHEQAVQEKL EDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMKPIAPLA
Sbjct: 301  ATWALAYLIHEQAVQEKLNEDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMKPIAPLA 360

Query: 1331 IPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDIWPEPYKFMPERFLQGEDGSANNKAM 1510
            IPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDIWPEPYKFMPERFLQGE+ SANNKAM
Sbjct: 361  IPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDIWPEPYKFMPERFLQGENVSANNKAM 420

Query: 1511 EQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 1630
            EQSFLPFGAGMRICAGM+LGKLQIAFALANLVNAFKWSCV
Sbjct: 421  EQSFLPFGAGMRICAGMDLGKLQIAFALANLVNAFKWSCV 460


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
            Full=(S)-tetrahydrocolumbamine oxidase
            (methylenedioxy-bridge-forming); AltName: Full=Cytochrome
            P450 719A1 gi|15822820|dbj|BAB68769.1| methylenedioxy
            bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  664 bits (1714), Expect = 0.0
 Identities = 323/425 (76%), Positives = 363/425 (85%)
 Frame = +2

Query: 356  WPSGPKRLPIIGNXXXXXXXXXXVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVL 535
            WPSGP++LPIIGN          V LAKLAKVHGSVMTIWIGSWRP+IVISDI  AWEVL
Sbjct: 36   WPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVL 95

Query: 536  VSKSAEFGQRDTPEIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFRE 715
            V+KSA++G RD PEI KI +   + I+ SD G FW NVRK LQ+GA+ PLNVAAQ Q++E
Sbjct: 96   VNKSADYGARDMPEITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQE 155

Query: 716  EDMKRLIHEMSREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDNFVESMLYEIEDV 895
             DMKRLI  MS EA N NG +KPLDH+KKNT RLL RLIFG  FD++ F+ESM YEIED+
Sbjct: 156  RDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDI 215

Query: 896  LRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQ 1075
            +R+SGYARL EAFYYAKYLPS KK  REA+L K R+EELVRPLLSS PPTNSYLYFL SQ
Sbjct: 216  IRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQ 275

Query: 1076 NYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIE 1255
            N+ +E+IIFC FEL+ LGVDSTSST TWALAYLI EQ  QEKLY+DIRMT+GDVDLVKIE
Sbjct: 276  NFEEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIE 335

Query: 1256 DLSKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDI 1435
            D++KLKYLQ VVKETMRMKPIAPLAIPHKTA +TTLMGTKVA+GT+IMVNLYALHHN++I
Sbjct: 336  DVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNI 395

Query: 1436 WPEPYKFMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAF 1615
            WP+PYKFMPERFL+GE G+A NKAMEQSFLPF AGMRICAGM+LGKLQ AFALANLVNAF
Sbjct: 396  WPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAF 455

Query: 1616 KWSCV 1630
            KWSCV
Sbjct: 456  KWSCV 460


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  657 bits (1696), Expect = 0.0
 Identities = 319/425 (75%), Positives = 363/425 (85%)
 Frame = +2

Query: 356  WPSGPKRLPIIGNXXXXXXXXXXVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVL 535
            WP+GP +LPIIGN          V LAKLA+VHGSVMT+WIG+WRPIIVISDI+ AWEVL
Sbjct: 37   WPTGPNKLPIIGNLHQLGGDVLHVALAKLAQVHGSVMTVWIGNWRPIIVISDIDKAWEVL 96

Query: 536  VSKSAEFGQRDTPEIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFRE 715
            V+KS+++G RD PEI KI +   + I+ SD G FW  VRK LQ+GA+ PLN+AAQ+QF+E
Sbjct: 97   VNKSSDYGARDMPEITKIASASWHTISTSDAGAFWQTVRKGLQSGAMGPLNIAAQSQFQE 156

Query: 716  EDMKRLIHEMSREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDNFVESMLYEIEDV 895
             DMKRLI  +  EA   N  +KPLDH+KKNT RLL RLIFG TFD+D FVESM +EI+D+
Sbjct: 157  RDMKRLIQALRDEAAQNNNIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFVESMHFEIDDI 216

Query: 896  LRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQ 1075
            +R+SGYARL EAFYYAKYLPS KK  REAYL K R+E+LV PLLSSNPPTNSYLYFL SQ
Sbjct: 217  IRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCRVEDLVSPLLSSNPPTNSYLYFLLSQ 276

Query: 1076 NYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIE 1255
             +S+E+IIFC FEL+ LGVDSTSST TWALAYLI EQA+QEKLY+D RMT+GDVDLVKIE
Sbjct: 277  RFSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTLGDVDLVKIE 336

Query: 1256 DLSKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDI 1435
            D+SKLKYLQAVVKETMRMKPIAPLAIPHKTA DTTLMG+KVA+GT+IMVNLYALHHN++I
Sbjct: 337  DVSKLKYLQAVVKETMRMKPIAPLAIPHKTAKDTTLMGSKVAKGTRIMVNLYALHHNQNI 396

Query: 1436 WPEPYKFMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAF 1615
            W  PYKFMPERFLQGEDGSA+NKAMEQSFLPF AGMRICAGM+LGKLQ AFALANLVNAF
Sbjct: 397  WTNPYKFMPERFLQGEDGSASNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAF 456

Query: 1616 KWSCV 1630
            KWSCV
Sbjct: 457  KWSCV 461


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  649 bits (1675), Expect = 0.0
 Identities = 325/475 (68%), Positives = 376/475 (79%), Gaps = 5/475 (1%)
 Frame = +2

Query: 221  LHSIVTISTEMEGSFLWLIGSXXXXXXXXXXXXRMXXXXXXXXXX--WPSGPKRLPIIGN 394
            L ++V +  EMEGS L L G+            R+            WPSGPK+LPIIGN
Sbjct: 3    LRNMVAMVAEMEGSLL-LAGATLAILLTISTIVRITSSPSSSFSQMTWPSGPKKLPIIGN 61

Query: 395  XXXXXXXXXXVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEFGQRDTP 574
                      VTLAKLAKVHG VMTIW+GSW PIIVISDINSAWEVLV+KSA++G R+  
Sbjct: 62   LHQLRGELLHVTLAKLAKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAREVT 121

Query: 575  ---EIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFREEDMKRLIHEM 745
               E+ +   +  +NI+ SD+GP WHN+RK LQ+GALSPL+VA+QTQ RE DMKR++HEM
Sbjct: 122  YLHELLRDSAMHPHNISSSDVGPLWHNIRKGLQSGALSPLSVASQTQHRERDMKRVVHEM 181

Query: 746  SREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDNFVESMLYEIEDVLRLSGYARLT 925
            SRE +N NGTIKPL H+K +TARLLGRLIFGP+F++  F+ESMLYEIED +R S Y  LT
Sbjct: 182  SREIVNNNGTIKPLVHIKNSTARLLGRLIFGPSFNDSKFIESMLYEIEDFIRTSSYTSLT 241

Query: 926  EAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFC 1105
             +FYYAKYLPSQ+K  REA L KYRIEELVRPLLSS PPTN+YLYFL SQN S+E+ IFC
Sbjct: 242  NSFYYAKYLPSQRKATREAKLGKYRIEELVRPLLSSKPPTNAYLYFLLSQNLSEEVTIFC 301

Query: 1106 TFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIEDLSKLKYLQA 1285
             FELF LGVDS SST TWALAY+I EQ  QEKLY+DIRMTIGDVDLVKIED++KLKYLQ+
Sbjct: 302  IFELFLLGVDSASSTTTWALAYMIREQEAQEKLYQDIRMTIGDVDLVKIEDVNKLKYLQS 361

Query: 1286 VVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDIWPEPYKFMPE 1465
            VVKETMRMKPIAPLA+PH    DTTLMGTK+ +GT++MVNLYALHH ED+W EP KFMPE
Sbjct: 362  VVKETMRMKPIAPLALPHNAGKDTTLMGTKIVKGTRVMVNLYALHHYEDVWLEPSKFMPE 421

Query: 1466 RFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 1630
            R+LQGEDGS  +KAMEQS LPFGAGMRICAGM+LGKLQ+AF LANLVNAFKWSCV
Sbjct: 422  RYLQGEDGSVTHKAMEQSLLPFGAGMRICAGMDLGKLQVAFVLANLVNAFKWSCV 476


>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
            Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
            stylopine synthase [Argemone mexicana]
          Length = 504

 Score =  551 bits (1420), Expect = e-154
 Identities = 269/427 (62%), Positives = 325/427 (76%), Gaps = 2/427 (0%)
 Frame = +2

Query: 356  WPSGPKRLPIIGNXXXXXXXXXXVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVL 535
            WP+GPK LPIIGN          V L  LAK +G+VMTIWIGSWRP+IV+SDI+ AWEVL
Sbjct: 48   WPTGPKTLPIIGNMNILGGTALHVVLHNLAKTYGNVMTIWIGSWRPVIVVSDIDRAWEVL 107

Query: 536  VSKSAEFGQRDTPEIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFRE 715
            V+KS+++  RD PEI K+ T     I+ SD GPFW N+RK LQN ALSP N+++Q++F+E
Sbjct: 108  VNKSSDYSARDMPEITKLATADWKTISSSDSGPFWTNLRKGLQNVALSPQNLSSQSKFQE 167

Query: 716  EDMKRLIHEMSREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDNFVESMLYEIEDV 895
             D+ + I  +  EA   NG +KPLDH+KK   RL+ RLI+G  FDND +VE M + IE++
Sbjct: 168  RDIIKTIQNLKEEAKMNNGIVKPLDHLKKAMVRLISRLIYGQDFDNDEYVEEMHHTIEEL 227

Query: 896  LRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQ 1075
            +R+SGYARL EAFYYAKYLPS KK  RE   A  R++ LVRPLLS N PTN+YL+FL+SQ
Sbjct: 228  IRVSGYARLAEAFYYAKYLPSHKKAVREVLQANQRVQNLVRPLLSLNSPTNTYLHFLRSQ 287

Query: 1076 NYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVD--LVK 1249
            NY  E+IIF  FE + LGVDSTSST  WALAYLI E  VQEKLYE+++    D D  +VK
Sbjct: 288  NYEDEVIIFAIFEAYLLGVDSTSSTTAWALAYLIREPNVQEKLYEELKNFTNDNDRKMVK 347

Query: 1250 IEDLSKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALHHNE 1429
             EDL+KL+YLQAVVKETMRMKPIAPLAIPHK   +T+LMG KV +GT++MVN+YALHHN+
Sbjct: 348  FEDLNKLQYLQAVVKETMRMKPIAPLAIPHKACRETSLMGRKVNQGTRVMVNIYALHHNQ 407

Query: 1430 DIWPEPYKFMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVN 1609
            ++W EPYKF PERFLQ      + KAMEQS LPF AGMRICAGM LGKLQ +FALANLVN
Sbjct: 408  NVWKEPYKFNPERFLQKNQDGVDGKAMEQSLLPFSAGMRICAGMELGKLQFSFALANLVN 467

Query: 1610 AFKWSCV 1630
            AFKWSCV
Sbjct: 468  AFKWSCV 474