BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00018712
         (1791 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270943.2| PREDICTED: sphingosine kinase B-like [Vitis ...   617   e-174
emb|CBI23326.3| unnamed protein product [Vitis vinifera]              617   e-174
ref|XP_002524674.1| Sphingosine kinase, putative [Ricinus commun...   592   e-166
ref|XP_002317243.1| predicted protein [Populus trichocarpa] gi|2...   588   e-165
ref|XP_003545841.1| PREDICTED: sphingosine kinase A-like [Glycin...   573   e-161

>ref|XP_002270943.2| PREDICTED: sphingosine kinase B-like [Vitis vinifera]
          Length = 489

 Score =  617 bits (1590), Expect = e-174
 Identities = 295/470 (62%), Positives = 369/470 (78%), Gaps = 1/470 (0%)
 Frame = +1

Query: 1    KLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKALVEKESWSCXXXXXXXXXXXXXXX 180
            K+RW+DG  +RC+ +E+EVLGF     EG  +R++A+VE     C               
Sbjct: 30   KIRWSDGHTDRCLAVEKEVLGFA---SEGSRIRIRAIVENGGGLCCVGSRGKLVR----- 81

Query: 181  XXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLLVIVNPFGGKRIALKIYNDEVKPL 360
               +DF+F+    DS +++CQK RE++DSL RPKRL V VNPFGGK+ A KI+ D V PL
Sbjct: 82   ---KDFVFEIASADSHRLWCQKLREYIDSLGRPKRLFVFVNPFGGKKSASKIFLDVVSPL 138

Query: 361  LDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCVSGDGILVEVVNGLLERKDWDTAI 540
            L  ADI+ T+QET++QLHAK + ++LDLS+YDG++CVSGDGILVEVVNGLLER DWD+AI
Sbjct: 139  LKDADIQLTIQETKHQLHAKAVTQTLDLSKYDGLICVSGDGILVEVVNGLLERDDWDSAI 198

Query: 541  KMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIVRGHKRALDVATVLQGETRFFSVL 720
            KMP+G++ AGTGNGM KSLLDSV + CS  NAVLAI+RGHK +LDVAT+LQGET+FFSVL
Sbjct: 199  KMPIGVIPAGTGNGMAKSLLDSVGDPCSPINAVLAIIRGHKCSLDVATILQGETKFFSVL 258

Query: 721  MLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLRKYNGRVSFVPAPGFEAYGNPCDQ 900
            MLAWGL+ADIDIESEKYRWMGS+RLDFY L R+ +LRKYNG +SFVPAPGFEA+G P   
Sbjct: 259  MLAWGLVADIDIESEKYRWMGSARLDFYALQRILHLRKYNGCISFVPAPGFEAFGEPSTY 318

Query: 901  KVGCTN-IDILFRDQQDTVKVQEHGYQGPVVLMEELKWRAVEGPFVSVWLHNVPWGSEDT 1077
            K   T+ ++     Q++ +KVQ+HGYQGP + +E+L+WR ++GPFVS+WLHNVPWG EDT
Sbjct: 319  KGESTSGLNTFNPSQEEPIKVQQHGYQGPNIELEKLQWRTIDGPFVSIWLHNVPWGGEDT 378

Query: 1078 MAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTHVRSPYVMYLKVKAFILEPGHRV 1257
            +AAPDA+FSDGYLDLI+I+DCPK  LL L+++LN G H++SPYVMY KVKAF+LEPG   
Sbjct: 379  LAAPDAKFSDGYLDLIIIRDCPKLPLLALMSELNKGNHIKSPYVMYFKVKAFVLEPGPCT 438

Query: 1258 GDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKITVDQGLATLFCP 1407
             DP+KGGIIDSDGEVLARG G YKC+ K LM Y  ++I VDQGLAT+F P
Sbjct: 439  EDPTKGGIIDSDGEVLARGNGTYKCDQKALMAYDNLQIAVDQGLATVFSP 488


>emb|CBI23326.3| unnamed protein product [Vitis vinifera]
          Length = 506

 Score =  617 bits (1590), Expect = e-174
 Identities = 295/470 (62%), Positives = 369/470 (78%), Gaps = 1/470 (0%)
 Frame = +1

Query: 1    KLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKALVEKESWSCXXXXXXXXXXXXXXX 180
            K+RW+DG  +RC+ +E+EVLGF     EG  +R++A+VE     C               
Sbjct: 47   KIRWSDGHTDRCLAVEKEVLGFA---SEGSRIRIRAIVENGGGLCCVGSRGKLVR----- 98

Query: 181  XXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLLVIVNPFGGKRIALKIYNDEVKPL 360
               +DF+F+    DS +++CQK RE++DSL RPKRL V VNPFGGK+ A KI+ D V PL
Sbjct: 99   ---KDFVFEIASADSHRLWCQKLREYIDSLGRPKRLFVFVNPFGGKKSASKIFLDVVSPL 155

Query: 361  LDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCVSGDGILVEVVNGLLERKDWDTAI 540
            L  ADI+ T+QET++QLHAK + ++LDLS+YDG++CVSGDGILVEVVNGLLER DWD+AI
Sbjct: 156  LKDADIQLTIQETKHQLHAKAVTQTLDLSKYDGLICVSGDGILVEVVNGLLERDDWDSAI 215

Query: 541  KMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIVRGHKRALDVATVLQGETRFFSVL 720
            KMP+G++ AGTGNGM KSLLDSV + CS  NAVLAI+RGHK +LDVAT+LQGET+FFSVL
Sbjct: 216  KMPIGVIPAGTGNGMAKSLLDSVGDPCSPINAVLAIIRGHKCSLDVATILQGETKFFSVL 275

Query: 721  MLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLRKYNGRVSFVPAPGFEAYGNPCDQ 900
            MLAWGL+ADIDIESEKYRWMGS+RLDFY L R+ +LRKYNG +SFVPAPGFEA+G P   
Sbjct: 276  MLAWGLVADIDIESEKYRWMGSARLDFYALQRILHLRKYNGCISFVPAPGFEAFGEPSTY 335

Query: 901  KVGCTN-IDILFRDQQDTVKVQEHGYQGPVVLMEELKWRAVEGPFVSVWLHNVPWGSEDT 1077
            K   T+ ++     Q++ +KVQ+HGYQGP + +E+L+WR ++GPFVS+WLHNVPWG EDT
Sbjct: 336  KGESTSGLNTFNPSQEEPIKVQQHGYQGPNIELEKLQWRTIDGPFVSIWLHNVPWGGEDT 395

Query: 1078 MAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTHVRSPYVMYLKVKAFILEPGHRV 1257
            +AAPDA+FSDGYLDLI+I+DCPK  LL L+++LN G H++SPYVMY KVKAF+LEPG   
Sbjct: 396  LAAPDAKFSDGYLDLIIIRDCPKLPLLALMSELNKGNHIKSPYVMYFKVKAFVLEPGPCT 455

Query: 1258 GDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKITVDQGLATLFCP 1407
             DP+KGGIIDSDGEVLARG G YKC+ K LM Y  ++I VDQGLAT+F P
Sbjct: 456  EDPTKGGIIDSDGEVLARGNGTYKCDQKALMAYDNLQIAVDQGLATVFSP 505


>ref|XP_002524674.1| Sphingosine kinase, putative [Ricinus communis]
            gi|223536035|gb|EEF37693.1| Sphingosine kinase, putative
            [Ricinus communis]
          Length = 479

 Score =  592 bits (1525), Expect = e-166
 Identities = 295/468 (63%), Positives = 355/468 (75%)
 Frame = +1

Query: 4    LRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKALVEKESWSCXXXXXXXXXXXXXXXX 183
            LRW D   +RC+T+E+EVLGF IE   G  +RVK +++     C                
Sbjct: 30   LRWTDRG-QRCLTVEKEVLGFVIE---GSKIRVKTIIDNGDGICCAGNSGALVR------ 79

Query: 184  XXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLLVIVNPFGGKRIALKIYNDEVKPLL 363
              +DF+FQ   EDS ++ C + R++LDSL RPKRLLV VNPFGGKR A KI+ D VKPLL
Sbjct: 80   --KDFVFQPLTEDSQRVLCNRLRDYLDSLGRPKRLLVFVNPFGGKRSASKIFFDTVKPLL 137

Query: 364  DAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCVSGDGILVEVVNGLLERKDWDTAIK 543
            + AD++ T+QET++QLHAKE+  +LD+S+YDG+VCVSGDGILVEVVNGLL R+DW  AIK
Sbjct: 138  EDADVQITLQETKHQLHAKEVTSTLDISKYDGIVCVSGDGILVEVVNGLLAREDWRDAIK 197

Query: 544  MPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIVRGHKRALDVATVLQGETRFFSVLM 723
            +PLG+V AGT NGM KSLLDSV E C  SNAVLAI+RGHK +LDVAT+LQGET+FFSVLM
Sbjct: 198  LPLGMVPAGTSNGMAKSLLDSVGEPCKASNAVLAIIRGHKCSLDVATILQGETKFFSVLM 257

Query: 724  LAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLRKYNGRVSFVPAPGFEAYGNPCDQK 903
            L+WGL+ADIDIESEKYRWMGS+R+DFY + R+F+LR YNG +SFVPAPGFE YG P    
Sbjct: 258  LSWGLVADIDIESEKYRWMGSARIDFYAVQRIFHLRHYNGCISFVPAPGFETYGVPTSYN 317

Query: 904  VGCTNIDILFRDQQDTVKVQEHGYQGPVVLMEELKWRAVEGPFVSVWLHNVPWGSEDTMA 1083
               T+       Q+  +K Q HGYQGP V +  L WR + GPFVS+WLHNVPWG ED MA
Sbjct: 318  AESTS------KQEQPLKTQ-HGYQGPDVNLVNLDWRMISGPFVSIWLHNVPWGGEDVMA 370

Query: 1084 APDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTHVRSPYVMYLKVKAFILEPGHRVGD 1263
            APDA+FSDGYLDLI+I+ CPK SLL L+T LN+G HV+SPYV+YLKVKAFILEPG R  D
Sbjct: 371  APDAKFSDGYLDLILIQQCPKLSLLALMTALNNGDHVKSPYVIYLKVKAFILEPGPRTDD 430

Query: 1264 PSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKITVDQGLATLFCP 1407
            P+KGGIID DGEVLARG G+YKC+ K LM Y  + + VDQGLATLF P
Sbjct: 431  PTKGGIIDVDGEVLARGNGSYKCDQKSLMVYDKLHMMVDQGLATLFSP 478


>ref|XP_002317243.1| predicted protein [Populus trichocarpa] gi|222860308|gb|EEE97855.1|
            predicted protein [Populus trichocarpa]
          Length = 494

 Score =  588 bits (1516), Expect = e-165
 Identities = 289/470 (61%), Positives = 359/470 (76%), Gaps = 1/470 (0%)
 Frame = +1

Query: 1    KLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKALVEKESWSCXXXXXXXXXXXXXXX 180
            KL+W     +  +T+E+EVLGF I+   G  + +KA+ EK                    
Sbjct: 31   KLQWTTEKGQHSLTVEKEVLGFTIQ---GSKIIIKAVTEKRD------GIFCSASAGGGA 81

Query: 181  XXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLLVIVNPFGGKRIALKIYNDEVKPL 360
               +DF+F+   ++S  ++CQ  R++++SL RPKRL + VNPFGGK+ ALKIY D VKPL
Sbjct: 82   LARKDFVFEPLSDESLHLWCQHLRDYINSLGRPKRLFIFVNPFGGKKSALKIYFDVVKPL 141

Query: 361  LDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCVSGDGILVEVVNGLLERKDWDTAI 540
            L+ AD++ T+QET+YQLHAKE+VR++DL++YDG+VCVSGDGILVEVVNGLLER+DW+ AI
Sbjct: 142  LEDADVQITVQETKYQLHAKEVVRAMDLTKYDGIVCVSGDGILVEVVNGLLEREDWNAAI 201

Query: 541  KMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIVRGHKRALDVATVLQGETRFFSVL 720
            KMPLG V AGTGNGMVKS LDS  E C+ SNAV+AI+RGHK +LDVAT+LQG+T+FFSVL
Sbjct: 202  KMPLGTVPAGTGNGMVKSTLDSAGEPCTASNAVVAIIRGHKCSLDVATILQGDTKFFSVL 261

Query: 721  MLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLRKYNGRVSFVPAPGFEAYGNPCDQ 900
            M AWGL+ADIDIESEKYRWMGS+RLDFYGL R+  LR+Y+GR+SFVPAPGFEAYG P   
Sbjct: 262  MFAWGLVADIDIESEKYRWMGSARLDFYGLQRMLCLRQYSGRISFVPAPGFEAYGEPTRY 321

Query: 901  KVGCTNIDILFR-DQQDTVKVQEHGYQGPVVLMEELKWRAVEGPFVSVWLHNVPWGSEDT 1077
                T+        Q+  VK +++ YQGP V +  L+WR + GPF+SVWLHNVPWG E T
Sbjct: 322  NGEFTSTQSSINPGQEQHVKAEQYSYQGPDVDLTNLEWRTINGPFISVWLHNVPWGGEGT 381

Query: 1078 MAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTHVRSPYVMYLKVKAFILEPGHRV 1257
            MAAPDA+F+DG LDLI+IKDCPK  LL L+T L+DG HV+SP+VMYLKVKAFILEPG R 
Sbjct: 382  MAAPDAKFADGNLDLILIKDCPKLGLLALMTNLSDGGHVKSPHVMYLKVKAFILEPGQRT 441

Query: 1258 GDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKITVDQGLATLFCP 1407
             DP++GGIID DGEVLARG G YK + K LMTY  ++IT+DQGLATLFCP
Sbjct: 442  KDPTRGGIIDIDGEVLARGNGTYKHDQKTLMTYDKLQITMDQGLATLFCP 491


>ref|XP_003545841.1| PREDICTED: sphingosine kinase A-like [Glycine max]
          Length = 488

 Score =  573 bits (1476), Expect = e-161
 Identities = 287/471 (60%), Positives = 353/471 (74%), Gaps = 2/471 (0%)
 Frame = +1

Query: 1    KLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKALVEKESWSCXXXXXXXXXXXXXXX 180
            +L W++G  +RC++IE++VLG       G  +++K+LVE     C               
Sbjct: 29   RLWWSEGI-QRCLSIEKDVLGI---VASGPYIKIKSLVETRDGCCITSAPARLVR----- 79

Query: 181  XXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLLVIVNPFGGKRIALKIYNDEVKPL 360
                D +F    E+S +++C K REF+DSL RPKRLLV VNPFGGK+ A KI+ ++VKPL
Sbjct: 80   ---NDVVFMPSSEESHRLWCHKLREFIDSLGRPKRLLVFVNPFGGKKSATKIFAEQVKPL 136

Query: 361  LDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCVSGDGILVEVVNGLLERKDWDTAI 540
             + A I+ T+QET++QLHAKE+ RSLD+++YDG+VCVSGDGILVEVVNGLL+R+DWDTAI
Sbjct: 137  FEDAHIQITVQETKHQLHAKEVARSLDITKYDGIVCVSGDGILVEVVNGLLQREDWDTAI 196

Query: 541  KMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIVRGHKRALDVATVLQGETRFFSVL 720
            KMPLG+V AGTGNGM KSLLDSV + C V+NAVLAI+RG KR LDVAT+ QGETRFFS+L
Sbjct: 197  KMPLGVVPAGTGNGMAKSLLDSVGDPCEVANAVLAIIRGSKRPLDVATITQGETRFFSIL 256

Query: 721  MLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLRKYNGRVSFVPAPGFEAYGNPCDQ 900
            MLAWGL+ADIDIESEKYRWMGS+RLDFYGL R+ NLR+Y G VSFVPAPG+EA+G P   
Sbjct: 257  MLAWGLVADIDIESEKYRWMGSARLDFYGLCRLLNLRQYIGCVSFVPAPGYEAFGEPTSY 316

Query: 901  KVGCTNIDILFRD--QQDTVKVQEHGYQGPVVLMEELKWRAVEGPFVSVWLHNVPWGSED 1074
                T       D  + +   +Q   Y GP + +E L WR + GPF+SVWLHNVPWG+ED
Sbjct: 317  PGKSTTSKGSNNDPSEAERANLQRLCYLGPEINLENLNWRVINGPFISVWLHNVPWGAED 376

Query: 1075 TMAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTHVRSPYVMYLKVKAFILEPGHR 1254
            TMAAPDA+FSDGYLDLI+IK+CP   LL ++++LN G HV+SPYV YLKVKAF L+PG R
Sbjct: 377  TMAAPDAKFSDGYLDLIIIKNCPTLPLLSMMSELNKGGHVKSPYVTYLKVKAFNLQPGPR 436

Query: 1255 VGDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKITVDQGLATLFCP 1407
              D  K GIIDSDGEVLARG+G YKCE K LM Y  ++ITVDQGLATLF P
Sbjct: 437  TKDQEKEGIIDSDGEVLARGKGTYKCEQKTLMAYDKLQITVDQGLATLFTP 487