BLASTX nr result
ID: Coptis25_contig00018610
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00018610
(1429 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004146161.1| PREDICTED: LOW QUALITY PROTEIN: DNA-directed... 305 3e-88
ref|XP_002285900.1| PREDICTED: DNA-directed RNA polymerase II su... 309 1e-81
ref|XP_004169177.1| PREDICTED: DNA-directed RNA polymerase II su... 305 2e-80
emb|CAA36736.1| DNA-directed RNA polymerase [Glycine max] 281 2e-80
ref|XP_003517152.1| PREDICTED: DNA-directed RNA polymerase II su... 301 4e-79
>ref|XP_004146161.1| PREDICTED: LOW QUALITY PROTEIN: DNA-directed RNA polymerase II
subunit RPB1-like [Cucumis sativus]
Length = 1832
Score = 305 bits (781), Expect(2) = 3e-88
Identities = 166/276 (60%), Positives = 186/276 (67%), Gaps = 6/276 (2%)
Frame = +3
Query: 3 RKVLSFDGSYVNYRHLAVLCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLGA 182
R V+SFDGSYVNYRHLA+LCDTMTYRGHLMAITRHGINRNDTGP+MRCSFEETVDILL A
Sbjct: 1373 RVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPMMRCSFEETVDILLDA 1432
Query: 183 AVYAETDHLRGVTENIMLGQLAPIGTGDCAMYLNDQMLLKSVDLQLPSYFEGGGFDFSVT 362
AVYAETDHLRGVTENIMLGQLAPIGTG CA+YLND+ML +++LQLPSY + G DF +T
Sbjct: 1433 AVYAETDHLRGVTENIMLGQLAPIGTGGCALYLNDEMLKNAIELQLPSYID--GLDFGMT 1490
Query: 363 PS-SHVTITPYHASFQ--DYSLSPNVRSSPITDASFSPYVGGMG---TSSAAWSPLSSGY 524
PS S ++ TPYH +Y LSPN+R SPI+DA FSPYVGGM TSS +SP S GY
Sbjct: 1491 PSRSPISGTPYHEGMMSPNYLLSPNLRLSPISDAQFSPYVGGMAFSPTSSPGYSPSSPGY 1550
Query: 525 NSSCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXX 704
+ S GY P +P Y P+S G P G S
Sbjct: 1551 SPSSPGYSPTSPGYSPTSPGYSPTSPGYSP------------TSPTYSPSSPGYSPTSPA 1598
Query: 705 XXXXLTAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
+YSPTSPSY T PSY SPSY SPSY
Sbjct: 1599 YSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1634
Score = 48.1 bits (113), Expect(2) = 3e-88
Identities = 27/43 (62%), Positives = 27/43 (62%)
Frame = +1
Query: 811 TSPSYILNSPLNSPTSTIYSDTSLEDSETSPGCISTSASYSPT 939
TSPSY SP SPTS YS TS S TSPG TS SYSPT
Sbjct: 1665 TSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPT 1707
Score = 59.7 bits (143), Expect(2) = 3e-11
Identities = 46/154 (29%), Positives = 58/154 (37%)
Frame = +3
Query: 351 FSVTPSSHVTITPYHASFQDYSLSPNVRSSPITDASFSPYVGGMGTSSAAWSPLSSGYNS 530
+S T S+ +P ++ S SP S T S+SP +S A+SP S Y+
Sbjct: 1606 YSPTSPSYSPTSPSYSPTSP-SYSPTSPSYSPTSPSYSPXSPAYSPTSPAYSPTSPAYSP 1664
Query: 531 SCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXXXX 710
+ Y P +PSY P+S P
Sbjct: 1665 TSPSYSPTSPSYSPTSPSYSP--------------------------------------- 1685
Query: 711 XXLTAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
AYSPTSP Y T PSY SPSY SPSY
Sbjct: 1686 -TSPAYSPTSPGYSPTSPSYSPTSPSYSPTSPSY 1718
Score = 35.8 bits (81), Expect(2) = 3e-11
Identities = 20/43 (46%), Positives = 24/43 (55%)
Frame = +1
Query: 811 TSPSYILNSPLNSPTSTIYSDTSLEDSETSPGCISTSASYSPT 939
TSP+Y SP SPTS YS +S S +SP S YSP+
Sbjct: 1747 TSPNYSPTSPSYSPTSPAYSPSSPTYSPSSPYNTGPSPDYSPS 1789
>ref|XP_002285900.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB1-like [Vitis
vinifera]
Length = 1848
Score = 309 bits (791), Expect = 1e-81
Identities = 170/276 (61%), Positives = 186/276 (67%), Gaps = 6/276 (2%)
Frame = +3
Query: 3 RKVLSFDGSYVNYRHLAVLCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLGA 182
R V+SFDGSYVNYRHLA+LCDTMTYRGHLMAITRHGINRNDTGP+MRCSFEETVDILL A
Sbjct: 1373 RVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPMMRCSFEETVDILLDA 1432
Query: 183 AVYAETDHLRGVTENIMLGQLAPIGTGDCAMYLNDQMLLKSVDLQLPSYFEGGGFDFSVT 362
AVYAETD LRGVTENIMLGQLAPIGTGDCA+YLNDQML +++LQLPSY E G DF +T
Sbjct: 1433 AVYAETDFLRGVTENIMLGQLAPIGTGDCALYLNDQMLQHAIELQLPSYME--GLDFGMT 1490
Query: 363 PS-SHVTITPYHASFQ--DYSLSPNVRSSPITDASFSPYVGGMG---TSSAAWSPLSSGY 524
PS S +T TPYH +Y LSPN+R SPITDA FSPYVGGM TSS +SP S GY
Sbjct: 1491 PSRSPITGTPYHDGMMSPNYLLSPNLRLSPITDAQFSPYVGGMAFSPTSSPGYSPSSPGY 1550
Query: 525 NSSCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXX 704
+ S GY P +P Y P+S G P G S
Sbjct: 1551 SPSSPGYSPTSPGYSPTSPGYSPTSPGYSP------------TSPTYSPSSPGYSPTSPA 1598
Query: 705 XXXXLTAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
+YSPTSPSY T PSY SPSY SPSY
Sbjct: 1599 YSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1634
>ref|XP_004169177.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB1-like [Cucumis
sativus]
Length = 1853
Score = 305 bits (781), Expect = 2e-80
Identities = 166/276 (60%), Positives = 186/276 (67%), Gaps = 6/276 (2%)
Frame = +3
Query: 3 RKVLSFDGSYVNYRHLAVLCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLGA 182
R V+SFDGSYVNYRHLA+LCDTMTYRGHLMAITRHGINRNDTGP+MRCSFEETVDILL A
Sbjct: 1373 RVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPMMRCSFEETVDILLDA 1432
Query: 183 AVYAETDHLRGVTENIMLGQLAPIGTGDCAMYLNDQMLLKSVDLQLPSYFEGGGFDFSVT 362
AVYAETDHLRGVTENIMLGQLAPIGTG CA+YLND+ML +++LQLPSY + G DF +T
Sbjct: 1433 AVYAETDHLRGVTENIMLGQLAPIGTGGCALYLNDEMLKNAIELQLPSYID--GLDFGMT 1490
Query: 363 PS-SHVTITPYHASFQ--DYSLSPNVRSSPITDASFSPYVGGMG---TSSAAWSPLSSGY 524
PS S ++ TPYH +Y LSPN+R SPI+DA FSPYVGGM TSS +SP S GY
Sbjct: 1491 PSRSPISGTPYHEGMMSPNYLLSPNLRLSPISDAQFSPYVGGMAFSPTSSPGYSPSSPGY 1550
Query: 525 NSSCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXX 704
+ S GY P +P Y P+S G P G S
Sbjct: 1551 SPSSPGYSPTSPGYSPTSPGYSPTSPGYSP------------TSPTYSPSSPGYSPTSPA 1598
Query: 705 XXXXLTAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
+YSPTSPSY T PSY SPSY SPSY
Sbjct: 1599 YSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1634
Score = 60.5 bits (145), Expect(2) = 2e-11
Identities = 47/154 (30%), Positives = 59/154 (38%)
Frame = +3
Query: 351 FSVTPSSHVTITPYHASFQDYSLSPNVRSSPITDASFSPYVGGMGTSSAAWSPLSSGYNS 530
+S T S+ +P ++ S SP S T S+SP +S A+SP S Y+
Sbjct: 1606 YSPTSPSYSPTSPSYSPTSP-SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSP 1664
Query: 531 SCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXXXX 710
+ Y P +PSY P+S P S
Sbjct: 1665 TSPSYSPTSPSYSPTSPSYSP-------------------TSPSYSPTSPSYSPTSPSYS 1705
Query: 711 XXLTAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
AYSPTSP Y T PSY SPSY SPSY
Sbjct: 1706 PTSPAYSPTSPGYSPTSPSYSPTSPSYSPTSPSY 1739
Score = 35.8 bits (81), Expect(2) = 2e-11
Identities = 20/43 (46%), Positives = 24/43 (55%)
Frame = +1
Query: 811 TSPSYILNSPLNSPTSTIYSDTSLEDSETSPGCISTSASYSPT 939
TSP+Y SP SPTS YS +S S +SP S YSP+
Sbjct: 1768 TSPNYSPTSPSYSPTSPAYSPSSPTYSPSSPYNTGPSPDYSPS 1810
Score = 57.8 bits (138), Expect = 7e-06
Identities = 45/151 (29%), Positives = 56/151 (37%)
Frame = +3
Query: 360 TPSSHVTITPYHASFQDYSLSPNVRSSPITDASFSPYVGGMGTSSAAWSPLSSGYNSSCR 539
+P T Y S YS P + T S+SP +S ++SP S Y+ +
Sbjct: 1575 SPGYSPTSPTYSPSSPGYS--PTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1632
Query: 540 GYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXXXXXXL 719
Y P +PSY P+S P S
Sbjct: 1633 SYSPTSPSYSPTSPAYSP-------------------TSPAYSPTSPAYSPTSPSYSPTS 1673
Query: 720 TAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
+YSPTSPSY T PSY SPSY SPSY
Sbjct: 1674 PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1704
>emb|CAA36736.1| DNA-directed RNA polymerase [Glycine max]
Length = 977
Score = 281 bits (719), Expect(2) = 2e-80
Identities = 156/278 (56%), Positives = 176/278 (63%), Gaps = 8/278 (2%)
Frame = +3
Query: 3 RKVLSFDGSYVNYRHLAVLCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLGA 182
R V+SFDGSYVNYRHLA+LC+TMTYRGHLMAITRHGINRNDTGP+MRCSFEETVDILL A
Sbjct: 565 RIVISFDGSYVNYRHLAILCETMTYRGHLMAITRHGINRNDTGPMMRCSFEETVDILLDA 624
Query: 183 AVYAETDHLRGVTENIMLGQLAPIGTGDCAMYLNDQMLLKSVDLQLPSYFEGGGFDFSVT 362
A YAETD LRGVTENI+LGQLAPIGTG CA+YLND ML +++LQLPSY + G DF +
Sbjct: 625 AAYAETDSLRGVTENIILGQLAPIGTGQCALYLNDGMLKNAIELQLPSYTD--GLDFGIM 682
Query: 363 PSSHVTITPYHASFQ---DYSLSPNVRSSPITDASFSPYVGGMG---TSSAAWSPLSSGY 524
+ TPYH Y LSPN+R SP +DA FSPYVGGM +SS +SP S GY
Sbjct: 683 SGA----TPYHEGTMMSPSYLLSPNLRLSPSSDAQFSPYVGGMAFSPSSSPGYSPSSPGY 738
Query: 525 NSSCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXX 704
+ S GY P +P Y P+S G P
Sbjct: 739 SPSSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPAYSPTS 798
Query: 705 XXXXLT--AYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
T AYSPTSPSY T P+Y SPSY L SPSY
Sbjct: 799 PAYSSTSPAYSPTSPSYSPTSPAYSPTSPSYSLTSPSY 836
Score = 45.8 bits (107), Expect(2) = 2e-80
Identities = 26/43 (60%), Positives = 26/43 (60%)
Frame = +1
Query: 811 TSPSYILNSPLNSPTSTIYSDTSLEDSETSPGCISTSASYSPT 939
TSPSY SP SPTS YS TS S TSP TS SYSPT
Sbjct: 832 TSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPT 874
>ref|XP_003517152.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB1-like [Glycine
max]
Length = 1827
Score = 301 bits (770), Expect = 4e-79
Identities = 163/276 (59%), Positives = 184/276 (66%), Gaps = 6/276 (2%)
Frame = +3
Query: 3 RKVLSFDGSYVNYRHLAVLCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLGA 182
R V+SFDGSYVNYRHLA+LCDTMTYRGHLMAITRHGINRNDTGP+MRCSFEETVDILL A
Sbjct: 1373 RVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPMMRCSFEETVDILLDA 1432
Query: 183 AVYAETDHLRGVTENIMLGQLAPIGTGDCAMYLNDQMLLKSVDLQLPSYFEGGGFDFSVT 362
AVYAETD+LRGVTENIMLGQLAPIGTG+CA+YLND+ML +++LQLPSY + G DF +T
Sbjct: 1433 AVYAETDYLRGVTENIMLGQLAPIGTGECALYLNDEMLKNAIELQLPSYMD--GLDFGMT 1490
Query: 363 PS-SHVTITPYHASFQ--DYSLSPNVRSSPITDASFSPYVGGMG---TSSAAWSPLSSGY 524
P+ S ++ TPYH Y LSPN+R SP TDA FSPYVGGM TSS +SP S GY
Sbjct: 1491 PARSPISGTPYHEGLMSPSYLLSPNLRLSPTTDAQFSPYVGGMAFSPTSSPGYSPSSPGY 1550
Query: 525 NSSCRGYIPNTPSYRPSSLGNDPYLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXX 704
+ S GY P +P Y P+S G P S
Sbjct: 1551 SPSSPGYSPTSPGYSPTSPGYSP-------------------TSPTYSPSSPGYSPTSPA 1591
Query: 705 XXXXLTAYSPTSPSYDQTRPSYILNSPSYILNSPSY 812
+YSPTSPSY T PSY SPSY SPSY
Sbjct: 1592 YSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSY 1627
Score = 59.3 bits (142), Expect(2) = 6e-11
Identities = 43/133 (32%), Positives = 50/133 (37%)
Frame = +3
Query: 414 SLSPNVRSSPITDASFSPYVGGMGTSSAAWSPLSSGYNSSCRGYIPNTPSYRPSSLGNDP 593
S SP S T S+SP +S A+SP S Y+ + Y P +PSY P+S P
Sbjct: 1605 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYSP 1664
Query: 594 YLLGXXXXXXXXXXXXXVIDSNILXXXXXXXXXXXXXXXXXLTAYSPTSPSYDQTRPSYI 773
S AYSPTSP Y T PSY
Sbjct: 1665 -------------------TSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYS 1705
Query: 774 LNSPSYILNSPSY 812
SPSY SPSY
Sbjct: 1706 PTSPSYSPTSPSY 1718
Score = 35.4 bits (80), Expect(2) = 6e-11
Identities = 20/43 (46%), Positives = 24/43 (55%)
Frame = +1
Query: 811 TSPSYILNSPLNSPTSTIYSDTSLEDSETSPGCISTSASYSPT 939
TSP+Y SP SPTS YS +S S +SP S YSP+
Sbjct: 1747 TSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYNSGVSPDYSPS 1789