BLASTX nr result
ID: Coptis25_contig00017404
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00017404
(793 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI38103.3| unnamed protein product [Vitis vinifera] 206 7e-70
ref|XP_002879527.1| eIF4-gamma/eIF5/eIF2-epsilon domain-containi... 175 3e-59
ref|NP_181042.1| translation initiation factor eIF-2B epsilon su... 174 6e-59
ref|XP_002264377.2| PREDICTED: LOW QUALITY PROTEIN: translation ... 204 1e-50
ref|XP_002328528.1| predicted protein [Populus trichocarpa] gi|2... 182 1e-43
>emb|CBI38103.3| unnamed protein product [Vitis vinifera]
Length = 730
Score = 206 bits (524), Expect(2) = 7e-70
Identities = 106/188 (56%), Positives = 133/188 (70%), Gaps = 8/188 (4%)
Frame = +2
Query: 254 TVLDMPNGQSTSKPS-----NSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXX 418
+ +D NG T K ++S+VG+ G+GYIWS+CEGGHEEEWRHSVAP PADK
Sbjct: 460 STVDKLNGVLTCKLPETEAWSTSEVGSGGVGYIWSICEGGHEEEWRHSVAPTPADKLTEV 519
Query: 419 XXXXXXXXXXXXXXGSVLPTSGELIPDSDI---VNSDNDDCKEDSVDFEKEVEATFLRAV 589
+VL SGEL+PDS+ + DN+D ++DSV FEKEVEATFLRAV
Sbjct: 520 MQATDDDLELTTQGSNVLLPSGELVPDSNSNISEDDDNEDSRDDSVYFEKEVEATFLRAV 579
Query: 590 HENIEQDHVIIEVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITK 769
HEN+++DHVI+EVNSLRLSYNM++ DCAGA+FYSVM+ A+ETP +NSEL NV VIT+
Sbjct: 580 HENVKEDHVILEVNSLRLSYNMTSADCAGALFYSVMKLALETPCNSNSELLKNVASVITR 639
Query: 770 WGGLLKHY 793
W L+K Y
Sbjct: 640 WQKLIKSY 647
Score = 84.7 bits (208), Expect(2) = 7e-70
Identities = 39/51 (76%), Positives = 48/51 (94%)
Frame = +3
Query: 3 IQDDCKVRHAIVCDGVVIKSGAVVEPGVILSFKVIIGQQFVVPAYSKVSLL 155
I+D C ++HAIVC+ V++KSGAV+EPGVILSFKV+IGQ+FVVPAYSKVSLL
Sbjct: 381 IEDGCVLKHAIVCNDVIMKSGAVLEPGVILSFKVVIGQEFVVPAYSKVSLL 431
>ref|XP_002879527.1| eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein [Arabidopsis
lyrata subsp. lyrata] gi|297325366|gb|EFH55786.1|
eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein
[Arabidopsis lyrata subsp. lyrata]
Length = 726
Score = 175 bits (443), Expect(2) = 3e-59
Identities = 89/177 (50%), Positives = 113/177 (63%)
Frame = +2
Query: 263 DMPNGQSTSKPSNSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXX 442
D +G + S +S++G G GYIW CEG H+EEW+HSVAPIP DK
Sbjct: 461 DHLSGLNLQMESKASELGPDGAGYIWEACEGAHDEEWKHSVAPIPKDKLFEITQAIDDDD 520
Query: 443 XXXXXXGSVLPTSGELIPDSDIVNSDNDDCKEDSVDFEKEVEATFLRAVHENIEQDHVII 622
SV+PTSGEL D+D +N+D +D +D FEKEVE T LRAV ENI+ D V +
Sbjct: 521 TDDE---SVVPTSGELKSDADSINTDVNDPNDDYYYFEKEVEGTVLRAVEENIKVDLVTL 577
Query: 623 EVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHY 793
E+N LRLSYNM + DCAGA FYS+++ A++TPH + SELY N +ITKW LL Y
Sbjct: 578 EINGLRLSYNMESADCAGATFYSMIKLAVDTPHNSASELYKNAASIITKWKELLGFY 634
Score = 80.5 bits (197), Expect(2) = 3e-59
Identities = 35/51 (68%), Positives = 47/51 (92%)
Frame = +3
Query: 3 IQDDCKVRHAIVCDGVVIKSGAVVEPGVILSFKVIIGQQFVVPAYSKVSLL 155
I+D C++R+AIVCDGV I++GAV++PGV+LSF V++G+ FVVPAYSKVSLL
Sbjct: 388 IEDGCEIRNAIVCDGVKIRAGAVLQPGVVLSFNVVVGRDFVVPAYSKVSLL 438
>ref|NP_181042.1| translation initiation factor eIF-2B epsilon subunit [Arabidopsis
thaliana] gi|3033392|gb|AAC12836.1| putative translation
initiation factor eIF-2B epsilon subunit [Arabidopsis
thaliana] gi|22655208|gb|AAM98194.1| putative
translation initiation factor eIF-2B epsilon subunit
[Arabidopsis thaliana] gi|34098787|gb|AAQ56776.1|
At2g34970 [Arabidopsis thaliana]
gi|330253950|gb|AEC09044.1| translation initiation
factor eIF-2B epsilon subunit [Arabidopsis thaliana]
Length = 730
Score = 174 bits (440), Expect(2) = 6e-59
Identities = 88/177 (49%), Positives = 114/177 (64%)
Frame = +2
Query: 263 DMPNGQSTSKPSNSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXX 442
D +G + S +S++G G GYIW VCEG H+EEW+HSVAPIP DK
Sbjct: 462 DHLSGLNLQMESKASELGPDGAGYIWEVCEGAHDEEWKHSVAPIPKDKLSEITQAIDDDD 521
Query: 443 XXXXXXGSVLPTSGELIPDSDIVNSDNDDCKEDSVDFEKEVEATFLRAVHENIEQDHVII 622
SV+PTSGEL D+D +N+D +D +D FEKEVE T LRAV ENI+ D V +
Sbjct: 522 TDDE---SVVPTSGELKSDADSINTDVNDPNDDYYYFEKEVEGTVLRAVEENIKVDLVTM 578
Query: 623 EVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHY 793
E+N LRLS+NM + DCAGA F+S+++ A++TPH + SELY N +ITKW LL Y
Sbjct: 579 EINGLRLSFNMESADCAGATFFSMIKLALDTPHNSGSELYKNAASIITKWKDLLGFY 635
Score = 80.5 bits (197), Expect(2) = 6e-59
Identities = 35/51 (68%), Positives = 47/51 (92%)
Frame = +3
Query: 3 IQDDCKVRHAIVCDGVVIKSGAVVEPGVILSFKVIIGQQFVVPAYSKVSLL 155
I+D C++R+AIVCDGV I++GAV++PGV+LSF V++G+ FVVPAYSKVSLL
Sbjct: 389 IEDGCEIRNAIVCDGVKIRAGAVLQPGVVLSFNVVVGRDFVVPAYSKVSLL 439
>ref|XP_002264377.2| PREDICTED: LOW QUALITY PROTEIN: translation initiation factor
eIF-2B subunit epsilon-like [Vitis vinifera]
Length = 458
Score = 204 bits (520), Expect = 1e-50
Identities = 101/165 (61%), Positives = 123/165 (74%), Gaps = 3/165 (1%)
Frame = +2
Query: 308 QVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXXXXXXXXGSVLPTSGE 487
QVG+ G+GYIWS+CEGGHEEEWRHSVAP PADK +VL SGE
Sbjct: 211 QVGSGGVGYIWSICEGGHEEEWRHSVAPTPADKLTEVMQATDDDLELTTQGSNVLLPSGE 270
Query: 488 LIPDSDI---VNSDNDDCKEDSVDFEKEVEATFLRAVHENIEQDHVIIEVNSLRLSYNMS 658
L+PDS+ + DN+D ++DSV FEKEVEATFLRAVHEN+++DHVI+EVNSLRLSYNM+
Sbjct: 271 LVPDSNSNISEDDDNEDSRDDSVYFEKEVEATFLRAVHENVKEDHVILEVNSLRLSYNMT 330
Query: 659 TLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHY 793
+ DCAGA+FYSVM+ A+ETP +NSEL NV VIT+W L+K Y
Sbjct: 331 SADCAGALFYSVMKLALETPCNSNSELLKNVASVITRWQKLIKSY 375
Score = 84.7 bits (208), Expect = 2e-14
Identities = 39/51 (76%), Positives = 48/51 (94%)
Frame = +3
Query: 3 IQDDCKVRHAIVCDGVVIKSGAVVEPGVILSFKVIIGQQFVVPAYSKVSLL 155
I+D C ++HAIVC+ V++KSGAV+EPGVILSFKV+IGQ+FVVPAYSKVSLL
Sbjct: 118 IEDGCVLKHAIVCNDVIMKSGAVLEPGVILSFKVVIGQEFVVPAYSKVSLL 168
>ref|XP_002328528.1| predicted protein [Populus trichocarpa] gi|222838243|gb|EEE76608.1|
predicted protein [Populus trichocarpa]
Length = 718
Score = 182 bits (461), Expect = 1e-43
Identities = 89/167 (53%), Positives = 115/167 (68%), Gaps = 3/167 (1%)
Frame = +2
Query: 302 SSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXXXXXXXXGSVLPTS 481
+SQ+G G+G++WS+CEGGHEEEWRHSVAPIPADK G+ L TS
Sbjct: 469 ASQLGPGGVGHVWSICEGGHEEEWRHSVAPIPADKLAEATQSLEDDLEFLNLDGNALSTS 528
Query: 482 GELIPDSDIVNSDNDDC---KEDSVDFEKEVEATFLRAVHENIEQDHVIIEVNSLRLSYN 652
GEL P + +S++DD ++DS+ +EKEVEATFLRAV+ENI+ VI+E+NSLRLSYN
Sbjct: 529 GELKPGRNGTDSEDDDAEDSRDDSIYYEKEVEATFLRAVNENIKVPDVILEMNSLRLSYN 588
Query: 653 MSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHY 793
M++ DCAGAIFY++M+ A+E PH EL N +I W LLK Y
Sbjct: 589 MTSADCAGAIFYAMMKQALEIPHATAGELRKNAASIIDAWNNLLKFY 635
Score = 89.0 bits (219), Expect = 1e-15
Identities = 39/50 (78%), Positives = 47/50 (94%)
Frame = +3
Query: 3 IQDDCKVRHAIVCDGVVIKSGAVVEPGVILSFKVIIGQQFVVPAYSKVSL 152
I+D C +RHAI+CDGVVIKSGA +EPGV+LSFKV+IGQQF+VP+YSKVSL
Sbjct: 383 IEDGCDIRHAIICDGVVIKSGAALEPGVVLSFKVVIGQQFIVPSYSKVSL 432