BLASTX nr result
ID: Coptis25_contig00017266
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00017266
(752 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004166389.1| PREDICTED: tRNA pseudouridine synthase A-lik... 137 6e-49
ref|XP_004137363.1| PREDICTED: tRNA pseudouridine synthase A-lik... 137 6e-49
ref|XP_002524161.1| pseudouridylate synthase, putative [Ricinus ... 129 2e-44
ref|XP_002331405.1| predicted protein [Populus trichocarpa] gi|2... 123 2e-43
ref|XP_002275354.2| PREDICTED: tRNA pseudouridine synthase A-lik... 122 8e-41
>ref|XP_004166389.1| PREDICTED: tRNA pseudouridine synthase A-like [Cucumis sativus]
Length = 444
Score = 137 bits (346), Expect(2) = 6e-49
Identities = 69/125 (55%), Positives = 86/125 (68%), Gaps = 8/125 (6%)
Frame = -2
Query: 697 MMEKQTEKKNYLHYNHTDSCNSARWNARECHEFMYSSRPWQNVVDFYSNVVNGKVSLSS- 521
+ME + ++K+YLHYNHTD C SARW ARE ++FMY RPWQ V+DFYS+ VNG+ SLSS
Sbjct: 22 LMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYG-RPWQEVLDFYSHAVNGRFSLSSL 80
Query: 520 ---SILFQNEDTKTCIDEVVEETKRRT----TGRWERVTFKILLSYHETSFNGWKKQPGL 362
I ++D +T + E R TGRW R TFKI+LSY SF+GW+KQPGL
Sbjct: 81 LGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARATFKIVLSYDGNSFDGWQKQPGL 140
Query: 361 NTVQG 347
NTVQG
Sbjct: 141 NTVQG 145
Score = 83.2 bits (204), Expect(2) = 6e-49
Identities = 38/56 (67%), Positives = 44/56 (78%)
Frame = -3
Query: 348 VERFLGRFVDDKKAEKLKDKCLPLEGCTTVEGRTDKGVTALKQACSFWVHQPSALP 181
VER LG+FVD+KKA+ LKDKCLPLEGC V GRTDKGVTAL+Q CSF+ + P
Sbjct: 147 VERSLGKFVDEKKAQMLKDKCLPLEGCALVAGRTDKGVTALRQVCSFYTWRTDVKP 202
Score = 76.3 bits (186), Expect = 7e-12
Identities = 39/66 (59%), Positives = 45/66 (68%)
Frame = -2
Query: 205 GPPTECITYHVQATEATLPCDNKVPGKGGERVKVMYSCVELVGNRFLRRMVRVLVSTSIR 26
GPPTEC YH +A E LPC + G+ CVELV NRFLR+MVRVLV+T+IR
Sbjct: 340 GPPTECFVYHARAAETRLPCLEQGVGRS-------VMCVELVANRFLRKMVRVLVATAIR 392
Query: 25 EAAAGA 8
EAAAGA
Sbjct: 393 EAAAGA 398
>ref|XP_004137363.1| PREDICTED: tRNA pseudouridine synthase A-like [Cucumis sativus]
Length = 444
Score = 137 bits (346), Expect(2) = 6e-49
Identities = 69/125 (55%), Positives = 86/125 (68%), Gaps = 8/125 (6%)
Frame = -2
Query: 697 MMEKQTEKKNYLHYNHTDSCNSARWNARECHEFMYSSRPWQNVVDFYSNVVNGKVSLSS- 521
+ME + ++K+YLHYNHTD C SARW ARE ++FMY RPWQ V+DFYS+ VNG+ SLSS
Sbjct: 22 LMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYG-RPWQEVLDFYSHAVNGRFSLSSL 80
Query: 520 ---SILFQNEDTKTCIDEVVEETKRRT----TGRWERVTFKILLSYHETSFNGWKKQPGL 362
I ++D +T + E R TGRW R TFKI+LSY SF+GW+KQPGL
Sbjct: 81 LGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARATFKIVLSYDGNSFDGWQKQPGL 140
Query: 361 NTVQG 347
NTVQG
Sbjct: 141 NTVQG 145
Score = 83.2 bits (204), Expect(2) = 6e-49
Identities = 38/56 (67%), Positives = 44/56 (78%)
Frame = -3
Query: 348 VERFLGRFVDDKKAEKLKDKCLPLEGCTTVEGRTDKGVTALKQACSFWVHQPSALP 181
VER LG+FVD+KKA+ LKDKCLPLEGC V GRTDKGVTAL+Q CSF+ + P
Sbjct: 147 VERSLGKFVDEKKAQMLKDKCLPLEGCALVAGRTDKGVTALRQVCSFYTWRTDVKP 202
Score = 76.3 bits (186), Expect = 7e-12
Identities = 39/66 (59%), Positives = 45/66 (68%)
Frame = -2
Query: 205 GPPTECITYHVQATEATLPCDNKVPGKGGERVKVMYSCVELVGNRFLRRMVRVLVSTSIR 26
GPPTEC YH +A E LPC + G+ CVELV NRFLR+MVRVLV+T+IR
Sbjct: 340 GPPTECFVYHARAAETRLPCLEQGVGRS-------VMCVELVANRFLRKMVRVLVATAIR 392
Query: 25 EAAAGA 8
EAAAGA
Sbjct: 393 EAAAGA 398
>ref|XP_002524161.1| pseudouridylate synthase, putative [Ricinus communis]
gi|223536579|gb|EEF38224.1| pseudouridylate synthase,
putative [Ricinus communis]
Length = 443
Score = 129 bits (325), Expect(2) = 2e-44
Identities = 68/123 (55%), Positives = 80/123 (65%), Gaps = 8/123 (6%)
Frame = -2
Query: 691 EKQTEKKNYLHYNHTDSCNSARWNARECHEFMYSSRPWQNVVDFYSNVVNGKVSL----S 524
E +T+ K Y HYNHTDSC S RW ARE +FMY+ RPWQ V DFYSNVVNG++S
Sbjct: 26 ETKTQNKIYFHYNHTDSCKSFRWTARESFQFMYA-RPWQEVSDFYSNVVNGRLSFLELFR 84
Query: 523 SSILFQNEDTKTCIDEVVEETKRRTT----GRWERVTFKILLSYHETSFNGWKKQPGLNT 356
S + F +D K D E + + GRW RV FKI LSYH SF+GW+KQPGLNT
Sbjct: 85 SQMHFVRDDAKIQEDSNKTELEHFSNEDRFGRWARVNFKIDLSYHGGSFDGWQKQPGLNT 144
Query: 355 VQG 347
VQG
Sbjct: 145 VQG 147
Score = 76.3 bits (186), Expect(2) = 2e-44
Identities = 36/65 (55%), Positives = 47/65 (72%)
Frame = -3
Query: 348 VERFLGRFVDDKKAEKLKDKCLPLEGCTTVEGRTDKGVTALKQACSFWVHQPSALPTMFK 169
VE+ LG+FVD+KKA++LK+ C PLEGC V GRTDKGV+AL+Q CSF+ T K
Sbjct: 149 VEKSLGKFVDEKKAQQLKEACKPLEGCAVVAGRTDKGVSALRQVCSFY--------TWRK 200
Query: 168 QLKPH 154
++PH
Sbjct: 201 DVRPH 205
Score = 85.9 bits (211), Expect = 8e-15
Identities = 46/69 (66%), Positives = 53/69 (76%)
Frame = -2
Query: 208 LGPPTECITYHVQATEATLPCDNKVPGKGGERVKVMYSCVELVGNRFLRRMVRVLVSTSI 29
+GPPTEC YH +ATEA LPC + GE KVM CVELV NRFLR+MVRVLV+TSI
Sbjct: 340 VGPPTECFIYHARATEARLPCPDH-----GEGRKVM--CVELVANRFLRKMVRVLVATSI 392
Query: 28 REAAAGADN 2
REAAAGA++
Sbjct: 393 REAAAGAED 401
>ref|XP_002331405.1| predicted protein [Populus trichocarpa] gi|222873619|gb|EEF10750.1|
predicted protein [Populus trichocarpa]
Length = 428
Score = 123 bits (308), Expect(2) = 2e-43
Identities = 68/134 (50%), Positives = 84/134 (62%), Gaps = 18/134 (13%)
Frame = -2
Query: 694 MEKQTEKKNYLHYNHTDSCNSARWNARECHEFMYSSRPWQNVVDFYSNVVNGKVSL---- 527
ME Q +K Y+ YNHTDSC +RW ARE +FM++ RPWQ VVDFYS VNG++SL
Sbjct: 1 MEAQNDKV-YVQYNHTDSCKFSRWTARESFQFMHA-RPWQEVVDFYSKSVNGQLSLLELF 58
Query: 526 SSSILFQNEDTKTC---IDEVVEETKRRT-----------TGRWERVTFKILLSYHETSF 389
+ + F E K D+ +EE T +GRW RVTFKI+LSYH SF
Sbjct: 59 GTQVFFTMELKKAHDVHCDDKIEEVSNETGLLEGVSNVDKSGRWARVTFKIVLSYHGGSF 118
Query: 388 NGWKKQPGLNTVQG 347
+GW+KQPGLNTVQG
Sbjct: 119 DGWQKQPGLNTVQG 132
Score = 79.3 bits (194), Expect(2) = 2e-43
Identities = 38/65 (58%), Positives = 49/65 (75%)
Frame = -3
Query: 348 VERFLGRFVDDKKAEKLKDKCLPLEGCTTVEGRTDKGVTALKQACSFWVHQPSALPTMFK 169
VE+ LGRFVD+KKA++LK++C PLEGC +V GRTDKGV+AL+Q CSF+ T K
Sbjct: 134 VEKSLGRFVDEKKAQQLKEQCKPLEGCASVAGRTDKGVSALQQVCSFY--------TWRK 185
Query: 168 QLKPH 154
+KPH
Sbjct: 186 DVKPH 190
Score = 77.4 bits (189), Expect = 3e-12
Identities = 40/67 (59%), Positives = 49/67 (73%)
Frame = -2
Query: 208 LGPPTECITYHVQATEATLPCDNKVPGKGGERVKVMYSCVELVGNRFLRRMVRVLVSTSI 29
+GPPTEC YH +ATE LP + G ++VM CVEL+ NRFLR+MVRVLV+TS+
Sbjct: 325 VGPPTECFLYHARATETRLPSPDHEKG-----IRVM--CVELIANRFLRKMVRVLVATSV 377
Query: 28 REAAAGA 8
REAAAGA
Sbjct: 378 REAAAGA 384
>ref|XP_002275354.2| PREDICTED: tRNA pseudouridine synthase A-like [Vitis vinifera]
Length = 433
Score = 122 bits (306), Expect(2) = 8e-41
Identities = 63/127 (49%), Positives = 78/127 (61%), Gaps = 10/127 (7%)
Frame = -2
Query: 697 MMEKQTEKKNYLHYNHTDSCNSARWNARECHEFMYSSRPWQNVVDFYSNVVNGKVSLSSS 518
+ME + E K Y+HY HT +RW ARE H+FMY RPWQ V+DFYSNVV G+ SL
Sbjct: 13 LMENEKENKVYVHYTHTHPTKFSRWTARESHQFMYK-RPWQQVLDFYSNVVTGRCSLLGL 71
Query: 517 ILFQNEDTKTCIDEVVEETKRRTT----------GRWERVTFKILLSYHETSFNGWKKQP 368
+ +T D + E +T GRW R+TFKI+LSYH SF+GW+KQP
Sbjct: 72 L---GIETHPIHDGEIVEASDKTEFGNVLTKDRGGRWARLTFKIVLSYHGASFDGWQKQP 128
Query: 367 GLNTVQG 347
GLNTVQG
Sbjct: 129 GLNTVQG 135
Score = 71.2 bits (173), Expect(2) = 8e-41
Identities = 33/48 (68%), Positives = 41/48 (85%)
Frame = -3
Query: 348 VERFLGRFVDDKKAEKLKDKCLPLEGCTTVEGRTDKGVTALKQACSFW 205
VE LGRFVD++KA++LK+K LP+EG TV GRTDKGVTAL+Q CSF+
Sbjct: 137 VEASLGRFVDERKAQQLKEKNLPVEGMVTVAGRTDKGVTALQQVCSFY 184
Score = 78.6 bits (192), Expect = 1e-12
Identities = 42/68 (61%), Positives = 50/68 (73%)
Frame = -2
Query: 208 LGPPTECITYHVQATEATLPCDNKVPGKGGERVKVMYSCVELVGNRFLRRMVRVLVSTSI 29
+GPPTEC +H +ATEA L + GE KVM C+ELV NRFLR+MVRVLV+TSI
Sbjct: 330 IGPPTECFVFHARATEARLQFSDD-----GEGTKVM--CIELVANRFLRKMVRVLVATSI 382
Query: 28 REAAAGAD 5
REAAAGA+
Sbjct: 383 REAAAGAE 390