BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00016709
         (1187 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like ...   543   e-152
emb|CBI40669.3| unnamed protein product [Vitis vinifera]              536   e-150
dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Pha...   526   e-147
ref|XP_002308090.1| predicted protein [Populus trichocarpa] gi|2...   523   e-146
ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|2...   521   e-145

>ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like [Vitis vinifera]
          Length = 748

 Score =  543 bits (1398), Expect = e-152
 Identities = 257/329 (78%), Positives = 293/329 (89%)
 Frame = -3

Query: 987 IRNASDGETAVVIEKPKVVREYKVFQGSPMPFGATAKDGGVNFAVHSSAAVSATLCLISL 808
           + + ++ ET VV+EKPK+ + + VF+G P P GATA+DGGVNFAV+S  AVSATLCLIS 
Sbjct: 11  VGSGAEAET-VVVEKPKL-QPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISA 68

Query: 807 TDLQENRVTKKINLDPLVNKTGDVWHAFLKGDFENMLYGYTLDGNFAPEEGFYYDSSRIL 628
           +DL+E+RVT++I+LDPL NKTGDVWH FLKG+FEN++YGY  DG F+PEEG YYDSSR+L
Sbjct: 69  SDLEEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLL 128

Query: 627 LDPYAKAVISREEFGSLGPGGNCWPQMAGMVPSSDNEFDWEGDLPLMYSQRDLIIYEMHV 448
           LDPYAKAVISR EFG LGP GNCWP MAGM+PSSD EFDWEGDLPL Y Q+DLIIYEMHV
Sbjct: 129 LDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHV 188

Query: 447 RGFTRHGSSGSNFPGTYLGVVEKLDHLKELGVNCIELMPFQEYNELEYFSYNSVMGNYKL 268
           RGFTRH SS + FPGTY GVVEKLDHLKELGVNCIELMP  E+NELEYFSYNSV+ +Y++
Sbjct: 189 RGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRV 248

Query: 267 NFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGN 88
           NFWGYSTVN+FSPM+RYSSAGI NCG DAINEVK+L+REAHKRGIEVLMDVVFNHTAEGN
Sbjct: 249 NFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEGN 308

Query: 87  EKGPILSFRGVDNSVFYMLAPKGEYYNYS 1
           E GPILSFRGVDNSV+YMLAPKGE+YNYS
Sbjct: 309 ENGPILSFRGVDNSVYYMLAPKGEFYNYS 337


>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score =  536 bits (1381), Expect = e-150
 Identities = 257/335 (76%), Positives = 293/335 (87%), Gaps = 6/335 (1%)
 Frame = -3

Query: 987  IRNASDGETAVVIEKPKVVREYKVFQGSPMPFGATAKDGGVNFAVHSSAAVSATLCLISL 808
            + + ++ ET VV+EKPK+ + + VF+G P P GATA+DGGVNFAV+S  AVSATLCLIS 
Sbjct: 66   VGSGAEAET-VVVEKPKL-QPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISA 123

Query: 807  TDLQENRVTKKINLDPLVNKTGDVWHAFLKGDFENMLYGYTLDGNFAPEEGFYYDSSRIL 628
            +DL+E+RVT++I+LDPL NKTGDVWH FLKG+FEN++YGY  DG F+PEEG YYDSSR+L
Sbjct: 124  SDLEEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLL 183

Query: 627  LDPYAKAVISREEFGSLGPGGNCWPQMAGMVPSSDNEFDWEGDLPLMYSQRDLIIYEMHV 448
            LDPYAKAVISR EFG LGP GNCWP MAGM+PSSD EFDWEGDLPL Y Q+DLIIYEMHV
Sbjct: 184  LDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHV 243

Query: 447  RGFTRHGSSGSNFPGTYLGVVEKLDHLKELGVNCIELMPFQEYNELEYFSYNSVMGNYK- 271
            RGFTRH SS + FPGTY GVVEKLDHLKELGVNCIELMP  E+NELEYFSYNSV+ +Y+ 
Sbjct: 244  RGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRY 303

Query: 270  -----LNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFN 106
                 +NFWGYSTVN+FSPM+RYSSAGI NCG DAINEVK+L+REAHKRGIEVLMDVVFN
Sbjct: 304  TCISMVNFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFN 363

Query: 105  HTAEGNEKGPILSFRGVDNSVFYMLAPKGEYYNYS 1
            HTAEGNE GPILSFRGVDNSV+YMLAPKGE+YNYS
Sbjct: 364  HTAEGNENGPILSFRGVDNSVYYMLAPKGEFYNYS 398


>dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Phaseolus vulgaris]
          Length = 791

 Score =  526 bits (1354), Expect = e-147
 Identities = 242/326 (74%), Positives = 283/326 (86%)
 Frame = -3

Query: 978  ASDGETAVVIEKPKVVREYKVFQGSPMPFGATAKDGGVNFAVHSSAAVSATLCLISLTDL 799
            A D ETAVV EKP++   ++V +G   PFGAT +DGGVNFA++S  A SATLCL +L+D 
Sbjct: 57   AVDTETAVV-EKPQLESLFQVSRGYASPFGATVRDGGVNFAIYSLNAFSATLCLFTLSDF 115

Query: 798  QENRVTKKINLDPLVNKTGDVWHAFLKGDFENMLYGYTLDGNFAPEEGFYYDSSRILLDP 619
            Q NRVT+ + LDPL+NKTG +WH FLKGDF +MLYGY  DG F+P EG YYDSSRI+LDP
Sbjct: 116  QNNRVTESVPLDPLINKTGGIWHVFLKGDFSDMLYGYKFDGKFSPLEGHYYDSSRIVLDP 175

Query: 618  YAKAVISREEFGSLGPGGNCWPQMAGMVPSSDNEFDWEGDLPLMYSQRDLIIYEMHVRGF 439
            YAKAVISR EFG+LGP GNCWPQMAGMVPS  +EFDWEGDLPL Y Q+DL++YEMHVRGF
Sbjct: 176  YAKAVISRGEFGALGPNGNCWPQMAGMVPSDHDEFDWEGDLPLKYPQKDLVVYEMHVRGF 235

Query: 438  TRHGSSGSNFPGTYLGVVEKLDHLKELGVNCIELMPFQEYNELEYFSYNSVMGNYKLNFW 259
            T+H SS + FPGTYLGVVEKLDHLKELGVNC+ELMP  E+NELEY SYNSV G+Y++NFW
Sbjct: 236  TKHESSNTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNELEYSSYNSVQGDYRVNFW 295

Query: 258  GYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKG 79
            GYST+NFFSPM+RYSSAGIRNCG+D INE+K L++EAHKRGIEV+MDVVFNHTAEGNE G
Sbjct: 296  GYSTINFFSPMIRYSSAGIRNCGRDGINEIKFLIKEAHKRGIEVIMDVVFNHTAEGNEDG 355

Query: 78   PILSFRGVDNSVFYMLAPKGEYYNYS 1
            PI+SFRGVDNS++YM+APKGE+YNYS
Sbjct: 356  PIISFRGVDNSIYYMVAPKGEFYNYS 381


>ref|XP_002308090.1| predicted protein [Populus trichocarpa] gi|222854066|gb|EEE91613.1|
            predicted protein [Populus trichocarpa]
          Length = 826

 Score =  523 bits (1346), Expect = e-146
 Identities = 251/333 (75%), Positives = 292/333 (87%), Gaps = 4/333 (1%)
 Frame = -3

Query: 987  IRNASDG-ETAVVI---EKPKVVREYKVFQGSPMPFGATAKDGGVNFAVHSSAAVSATLC 820
            I+ AS+G +TAVV+   E+PK+ ++ +VF+G P PFGAT +DGGVNFA+ S+ AVSATLC
Sbjct: 54   IKAASEGVDTAVVVVEEEEPKL-KKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLC 112

Query: 819  LISLTDLQENRVTKKINLDPLVNKTGDVWHAFLKGDFENMLYGYTLDGNFAPEEGFYYDS 640
            LISL+DL ENRVT++I LDPL NKTGDVWH  LKGDF++MLYGY  DGNF+PE G YYD 
Sbjct: 113  LISLSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDP 172

Query: 639  SRILLDPYAKAVISREEFGSLGPGGNCWPQMAGMVPSSDNEFDWEGDLPLMYSQRDLIIY 460
            S+I+LDPYAK+VISR EFG LG   N WPQMA M+P+++N+FDWEGD PL + QRDLIIY
Sbjct: 173  SKIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIY 232

Query: 459  EMHVRGFTRHGSSGSNFPGTYLGVVEKLDHLKELGVNCIELMPFQEYNELEYFSYNSVMG 280
            EMHVRGFT+H SS + FPGTYLGVVEKLDHLKELGVNCIELMP  E+NELEY+SYNSV+G
Sbjct: 233  EMHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLG 292

Query: 279  NYKLNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHT 100
            +YK+NFWGYSTVN+FSPM RYSSAG RNCG+DAINE K+LVREAHKRGIEV MDVVFNHT
Sbjct: 293  DYKVNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEVFMDVVFNHT 352

Query: 99   AEGNEKGPILSFRGVDNSVFYMLAPKGEYYNYS 1
            AEGNEKGPILSFRGVDNS++YMLAPKGE+YNYS
Sbjct: 353  AEGNEKGPILSFRGVDNSIYYMLAPKGEFYNYS 385


>ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|223530627|gb|EEF32503.1|
            isoamylase, putative [Ricinus communis]
          Length = 795

 Score =  521 bits (1342), Expect = e-145
 Identities = 245/323 (75%), Positives = 283/323 (87%), Gaps = 1/323 (0%)
 Frame = -3

Query: 966  ETAVVIEKPKVVREYKVFQGSPMPFGATAKDGGVNFAVHSSAAVSATLCLISLTDLQENR 787
            ETAVV++KPK  R Y+V +G P PFGAT  DGGVNFA++SS AVSA+LCLISL DL +NR
Sbjct: 66   ETAVVVDKPKF-RRYQVSEGHPAPFGATIADGGVNFAIYSSDAVSASLCLISLDDLTQNR 124

Query: 786  VTKKINLDPLVNKTGDVWHAFLKGDFENMLYGYTLDGNFAPEEGFYYDSSRILLDPYAKA 607
            V+++I LDP+ N+TGD+WH FLKGDF++ LYGY  DG  +P +G Y+DSS+I+LDPYAKA
Sbjct: 125  VSEEIALDPVRNRTGDIWHVFLKGDFKDTLYGYRFDGVLSPHDGHYFDSSQIVLDPYAKA 184

Query: 606  VISREEFGSLGPGGNCWPQMAGMVPS-SDNEFDWEGDLPLMYSQRDLIIYEMHVRGFTRH 430
            VISR EFG LGPG NCWPQMAGM+PS S  EFDWEGDLPL Y Q+DL+IYEMHVRGFTRH
Sbjct: 185  VISRGEFGVLGPGDNCWPQMAGMIPSASQAEFDWEGDLPLKYPQKDLVIYEMHVRGFTRH 244

Query: 429  GSSGSNFPGTYLGVVEKLDHLKELGVNCIELMPFQEYNELEYFSYNSVMGNYKLNFWGYS 250
             SS + FPGT+L  VEKLDHLKELGVNCIELMP  E+NELEY+SYNSV+G+YK+NFWGYS
Sbjct: 245  ESSRTEFPGTFLATVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYS 304

Query: 249  TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPIL 70
            T+N+FSPM RYSSAG RNCG+DAINE K LVREAHKRGIEV+MDVVFNHTAEGN+KGPIL
Sbjct: 305  TINYFSPMTRYSSAGTRNCGRDAINEFKFLVREAHKRGIEVIMDVVFNHTAEGNQKGPIL 364

Query: 69   SFRGVDNSVFYMLAPKGEYYNYS 1
            SFRGVDNSV+YMLAPKGE+YNYS
Sbjct: 365  SFRGVDNSVYYMLAPKGEFYNYS 387