BLASTX nr result
ID: Coptis25_contig00016501
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00016501
(1777 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABK25130.1| unknown [Picea sitchensis] 464 e-128
ref|XP_003609250.1| Cytochrome P450 [Medicago truncatula] gi|355... 420 e-115
ref|XP_004169401.1| PREDICTED: cytochrome P450 734A1-like [Cucum... 419 e-114
ref|XP_004139422.1| PREDICTED: cytochrome P450 734A1-like [Cucum... 419 e-114
dbj|BAF56240.1| cytochrome P450 enzyme [Pisum sativum] 417 e-114
>gb|ABK25130.1| unknown [Picea sitchensis]
Length = 517
Score = 464 bits (1194), Expect = e-128
Identities = 227/503 (45%), Positives = 332/503 (66%), Gaps = 12/503 (2%)
Frame = -2
Query: 1692 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1513
V + + WWKP +++K F +QG+ GPPY LL G+F ++ ++ + T +
Sbjct: 19 VLRIVTVIWWKPLQVKKCFESQGVRGPPYRLLNGNFADMVRMNTQAKSTPIP-------W 71
Query: 1512 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVTEPELIKEMLNNTNTYFKSIP-HPA 1336
H IV +LP+ H W + YG+ F+YW G PR+ V PELIKE+L+N ++++I +P
Sbjct: 72 SHDIVPRVLPYYHHWTKTYGKDFIYWVGSKPRLNVPHPELIKEILSNKFGHYENISSNPL 131
Query: 1335 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEIIERWEESINSDAP 1156
+VG GL+ G+KW +HRRI++P F + LKG V TIV ST ++E+W + + S A
Sbjct: 132 GRQLVGQGLVGLRGEKWVQHRRIINPAFHLDFLKGMVPTIVESTANMLEKWGKLVLSGAE 191
Query: 1155 EFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFLPTP 976
E EV +F LT ++I+RTA S++ + I LQ QQM + + + + Y+PG RFLPT
Sbjct: 192 EVEVLKEFCNLTADVISRTALGSSYAEAKHIFNLQDQQMLLTAELVLSVYVPGFRFLPTR 251
Query: 975 FNIKSWRVRRELEEMFRELIKSRDESIQ-------DNDLLGLMLSATNT---GNDSRKNM 826
N + W++ +E+ + R++I++R+ + DLLGLM+SA N G M
Sbjct: 252 KNRQRWKLEKEIRKCMRQVIEARERTADIEQSGSYGTDLLGLMMSAKNKRVGGKLQDVRM 311
Query: 825 SSKELIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVL-QGKLPDYETL 649
+++E+IDEC+TF+ AG ETTS+L+TW IL+G+H DWQD+ R EV +V + +PD +++
Sbjct: 312 TTEEIIDECKTFYFAGHETTSILLTWTIILLGMHQDWQDRGRKEVLEVCGKNVVPDADSV 371
Query: 648 GSLKIVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGN 469
LKIV MILNE LRLY PA F+ + K ++LG L+IPAGT L +P++A+HHD WGN
Sbjct: 372 NHLKIVGMILNEALRLYPPAVFLQRQAVKPMQLGRLSIPAGTQLLLPILAIHHDQCLWGN 431
Query: 468 DVDEFNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVIS 289
D +EFNP RF+EG+AKA HP+AFMPF G R+C+G NF+++EAK+ L+MILQ+FSFV S
Sbjct: 432 DANEFNPARFSEGIAKAVKHPLAFMPFGFGPRICVGQNFALLEAKVVLAMILQRFSFVTS 491
Query: 288 PSYKHSPDCLVTTSPMHGAHIIL 220
PSY H+P +VT P HGA +IL
Sbjct: 492 PSYAHAPVMVVTVRPQHGAQVIL 514
>ref|XP_003609250.1| Cytochrome P450 [Medicago truncatula] gi|355510305|gb|AES91447.1|
Cytochrome P450 [Medicago truncatula]
Length = 530
Score = 420 bits (1079), Expect = e-115
Identities = 210/506 (41%), Positives = 327/506 (64%), Gaps = 12/506 (2%)
Frame = -2
Query: 1692 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1513
V K V WW+PRKIE F QGI GPPY+ +G+ +EL + + QP+ +
Sbjct: 22 VLKVTVLLWWRPRKIEGYFAKQGIRGPPYHFFIGNVKELVGMMLKASS-----QPM-PNF 75
Query: 1512 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVTEPELIKEMLNNTNTYF-KSIPHPA 1336
H I+ +L H W++ YG +FL WFGP+ R+ V++P+LI+E+ + + ++ K+ P
Sbjct: 76 SHNILPRVLSFYHHWKKIYGGMFLVWFGPTVRLTVSDPDLIREIFTSKSEFYEKNEAPPL 135
Query: 1335 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEIIERWEESINSDAP 1156
V + GDGL+ +G+KWA HR+I+SP F E LK + + +S E++E+W E +
Sbjct: 136 VKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLIPVMATSVVEMLEKWSEMSDKGEV 195
Query: 1155 EFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFLPTP 976
E EV F+TLT+++I +TAF S+++ I LQ QQM + A + +IPG RF PT
Sbjct: 196 EIEVSEWFQTLTEDVITKTAFGSSYQDGKAIFHLQAQQMILAADAFQKIFIPGYRFFPTR 255
Query: 975 FNIKSWRVRRELEEMFRELIKSRDESIQDN----------DLLGLMLSATNTGNDSRKNM 826
NIKSW++ +E+++ +LIK R+E++ + DLLGLM+ A++ N++ N+
Sbjct: 256 KNIKSWKLDKEIKKSLVKLIKRREENLNNGNEERIEKGPKDLLGLMIEASSNNNNT--NV 313
Query: 825 SSKELIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETL 649
+ ++++EC++FF AG +TTS L+TW IL+ +H WQ +AR+EV ++ + LP + +
Sbjct: 314 TVDDIVEECKSFFFAGKQTTSNLLTWTTILLAMHPQWQVQARDEVLKMCGSRDLPTKDHV 373
Query: 648 GSLKIVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGN 469
LK +NMI+NE+LRLY P ++ + +V+LG IP GT L IP++AVHHD WGN
Sbjct: 374 VKLKTLNMIVNESLRLYPPTIATIRRAKTDVDLGGYKIPRGTELLIPILAVHHDIAIWGN 433
Query: 468 DVDEFNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVIS 289
DV+EFNP RF+EGVA+A+ HP+AF+PF G R CIG N ++++ K+AL++ILQ+ SF ++
Sbjct: 434 DVNEFNPGRFSEGVARAAKHPVAFIPFGLGVRTCIGQNLAVLQTKLALAIILQRLSFRLA 493
Query: 288 PSYKHSPDCLVTTSPMHGAHIILRRI 211
PSY+H+P L+ P +GA II ++
Sbjct: 494 PSYQHAPTVLMLLYPQYGAPIIFNQL 519
>ref|XP_004169401.1| PREDICTED: cytochrome P450 734A1-like [Cucumis sativus]
Length = 520
Score = 419 bits (1076), Expect = e-114
Identities = 215/502 (42%), Positives = 324/502 (64%), Gaps = 8/502 (1%)
Frame = -2
Query: 1692 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1513
V + +V WW+PR+IE+ F QGI GPPY +G+ +EL + KA +D +
Sbjct: 15 VLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMI-KASSDHSFP----NS 69
Query: 1512 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVTEPELIKEMLNNTNTYF-KSIPHPA 1336
H I+ +LP H W++ YG FL WFGP+ R+ V++P+LI+E+ + + + K+ PHP
Sbjct: 70 SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 129
Query: 1335 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEIIERWEE---SINS 1165
V + GDGL+ +G KWA HR+I+SP F + LK + + S +++E+W S +S
Sbjct: 130 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMTSADS 189
Query: 1164 DAPEFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFL 985
D E EV F+TLT+++I RTAF S+++ I LQ QQM + +QA +IPG RFL
Sbjct: 190 DEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 249
Query: 984 PTPFNIKSWRVRRELEEMFRELI-KSRDESIQDN--DLLGLMLSATNTGNDSRKNMSSKE 814
PT N+ SWR+ +E+ + +LI + R+ SI+ + DLLGLM+ A+ + S ++ +
Sbjct: 250 PTRTNVNSWRLDKEIRKSLMKLIDRRRENSIETSSKDLLGLMIRASKSSPSS--TITVND 307
Query: 813 LIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETLGSLK 637
+++EC+ FF AG +TTS L+TW ILM +H WQ +AR+EV + + +P + + LK
Sbjct: 308 IVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVLRECGARDIPSKDDVTKLK 367
Query: 636 IVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGNDVDE 457
+++MI+NE+LRLY P ++ + +VELG +P GT L IP++AVHHD WGNDV+E
Sbjct: 368 MLSMIINESLRLYPPTVATIRQAKVDVELGGYMLPRGTELLIPILAVHHDQTIWGNDVNE 427
Query: 456 FNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVISPSYK 277
FNP RFAEGVAKA+ H + F+PF G R CIG N ++++AK+AL++ILQ+FSF + PSY+
Sbjct: 428 FNPARFAEGVAKAANHRVGFIPFGLGARTCIGQNLAILQAKLALALILQRFSFRLGPSYQ 487
Query: 276 HSPDCLVTTSPMHGAHIILRRI 211
HSP + P HGA II +++
Sbjct: 488 HSPAVQMLLYPRHGAPIIFKKL 509
>ref|XP_004139422.1| PREDICTED: cytochrome P450 734A1-like [Cucumis sativus]
Length = 520
Score = 419 bits (1076), Expect = e-114
Identities = 215/502 (42%), Positives = 324/502 (64%), Gaps = 8/502 (1%)
Frame = -2
Query: 1692 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1513
V + +V WW+PR+IE+ F QGI GPPY +G+ +EL + KA +D +
Sbjct: 15 VLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMI-KASSDHSFP----NS 69
Query: 1512 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVTEPELIKEMLNNTNTYF-KSIPHPA 1336
H I+ +LP H W++ YG FL WFGP+ R+ V++P+LI+E+ + + + K+ PHP
Sbjct: 70 SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPL 129
Query: 1335 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEIIERWEE---SINS 1165
V + GDGL+ +G KWA HR+I+SP F + LK + + S +++E+W S +S
Sbjct: 130 VKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMTSADS 189
Query: 1164 DAPEFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFL 985
D E EV F+TLT+++I RTAF S+++ I LQ QQM + +QA +IPG RFL
Sbjct: 190 DEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPGYRFL 249
Query: 984 PTPFNIKSWRVRRELEEMFRELI-KSRDESIQDN--DLLGLMLSATNTGNDSRKNMSSKE 814
PT N+ SWR+ +E+ + +LI + R+ SI+ + DLLGLM+ A+ + S ++ +
Sbjct: 250 PTRTNVNSWRLDKEIRKSLMKLIDRRRENSIETSSKDLLGLMIRASKSSPSS--TITVND 307
Query: 813 LIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETLGSLK 637
+++EC+ FF AG +TTS L+TW ILM +H WQ +AR+EV + + +P + + LK
Sbjct: 308 IVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVLRECGARDIPSKDDVTKLK 367
Query: 636 IVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGNDVDE 457
+++MI+NE+LRLY P ++ + +VELG +P GT L IP++AVHHD WGNDV+E
Sbjct: 368 MLSMIINESLRLYPPTVATIRQAKVDVELGGYMLPRGTELLIPILAVHHDQTIWGNDVNE 427
Query: 456 FNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVISPSYK 277
FNP RFAEGVAKA+ H + F+PF G R CIG N ++++AK+AL++ILQ+FSF + PSY+
Sbjct: 428 FNPARFAEGVAKAANHRVGFIPFGLGARTCIGQNLAILQAKLALALILQRFSFRLGPSYQ 487
Query: 276 HSPDCLVTTSPMHGAHIILRRI 211
HSP + P HGA II +++
Sbjct: 488 HSPAVQMLLYPRHGAPIIFKKL 509
>dbj|BAF56240.1| cytochrome P450 enzyme [Pisum sativum]
Length = 523
Score = 417 bits (1072), Expect = e-114
Identities = 211/502 (42%), Positives = 320/502 (63%), Gaps = 8/502 (1%)
Frame = -2
Query: 1692 VNKFLVFAWWKPRKIEKQFRNQGINGPPYNLLVGSFRELAKIYSSKAKTDVVVQPLGSHM 1513
V K V WW+PRKIE F QGI GPPY +G+ +EL + + QP+ +
Sbjct: 21 VLKLTVLLWWRPRKIEGYFSKQGIRGPPYRFFIGNVKELVAMMIQASS-----QPM-PNF 74
Query: 1512 KHTIVQDLLPHLHLWREKYGRVFLYWFGPSPRVVVTEPELIKEMLNNTNTYF-KSIPHPA 1336
H I+ +L H W++ YG FL WFGP+ R+ V++P+LI+E+ + + ++ K+ P
Sbjct: 75 SHNILPRVLSFYHHWKKIYGGTFLVWFGPTVRLTVSDPDLIREIFTSKSEFYEKNEAPPL 134
Query: 1335 VYDMVGDGLIFAEGDKWARHRRILSPFFTTEALKGYVHTIVSSTNEIIERWEESINSDAP 1156
V + GDGL+ +G+KWA HRRI+SP F E LK + + +S E++E W E +
Sbjct: 135 VKQLEGDGLLSLKGEKWAHHRRIISPTFHMENLKLLIPVMATSVVEMLENWSEMSHKGEV 194
Query: 1155 EFEVHSQFRTLTKNIIARTAFQSNFKQVDRIVELQIQQMAMLTQALRTFYIPGSRFLPTP 976
E EV F+TLT+++I +TAF S+++ I LQ QQM + A + +IPG RF PT
Sbjct: 195 EIEVSECFQTLTEDVITKTAFGSSYQDGKAIFRLQAQQMVLAADAFQKVFIPGYRFFPTR 254
Query: 975 FNIKSWRVRRELEEMFRELIKSRDESIQDN------DLLGLMLSATNTGNDSRKNMSSKE 814
NIKSW++ +++++ +LI+ R E+ + DLLGLM+ A+N + N++ +
Sbjct: 255 RNIKSWKLDKQIKKSLVKLIERRRENSNERIEKGPKDLLGLMIQASN-----KTNVTVDD 309
Query: 813 LIDECRTFFVAGFETTSLLVTWAFILMGIHTDWQDKARNEVEQVLQGK-LPDYETLGSLK 637
++ EC++FF AG +TTS L+TW IL+ +H WQ +AR+EV ++ + +P + + LK
Sbjct: 310 IVGECKSFFFAGKQTTSNLLTWTTILLAMHPQWQVQARDEVLKMCGSRDVPTKDHVVKLK 369
Query: 636 IVNMILNETLRLYSPAPFIMKTVEKEVELGNLTIPAGTNLEIPLIAVHHDPHQWGNDVDE 457
+NMI+NE+LRLY P ++ + +VELG IP GT L IP++AVHHD WGNDV+E
Sbjct: 370 TLNMIVNESLRLYPPTIATIRRAKTDVELGGYKIPRGTELLIPILAVHHDQAIWGNDVNE 429
Query: 456 FNPDRFAEGVAKASTHPMAFMPFTSGTRVCIGMNFSMMEAKIALSMILQKFSFVISPSYK 277
FNP RF+EGVA+A+ HP+AF+PF G R CIG N ++++ K+AL MILQ+FSF ++PSY+
Sbjct: 430 FNPGRFSEGVARAAKHPVAFIPFGLGVRTCIGQNLAVLQTKLALVMILQRFSFRLAPSYQ 489
Query: 276 HSPDCLVTTSPMHGAHIILRRI 211
H+P L+ P +GA II +++
Sbjct: 490 HAPTVLMLLYPQYGAPIIFKQL 511