BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00016434
         (401 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             106   2e-31
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              107   1e-29
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   107   1e-29
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...    95   6e-29
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]                   92   5e-27

>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  106 bits (264), Expect(2) = 2e-31
 Identities = 48/75 (64%), Positives = 59/75 (78%)
 Frame = -2

Query: 226 SPSTTVAALAKLQDINCLNYLR*LLEKSGNAVPAAYPQDIYTELCMVDSLERLGMDRHFK 47
           SPSTT AA   L++  CL+YL  LL++ GNAVP  YP DIYT LCMVDSLERLG+DRHF+
Sbjct: 233 SPSTTAAAFTYLKNSGCLSYLHSLLDRFGNAVPTVYPLDIYTRLCMVDSLERLGIDRHFR 292

Query: 46  NKIRNVLERTYSCWL 2
            +I++VL+ TY CWL
Sbjct: 293 KEIKSVLDETYRCWL 307



 Score = 54.3 bits (129), Expect(2) = 2e-31
 Identities = 25/38 (65%), Positives = 29/38 (76%)
 Frame = -3

Query: 342 GSNSEGGNLYLAYVAEGLGKSQNWKDIWKLQKKNGSLF 229
           GSN EG   YLAY +EGLGKS +W+ I K Q+KNGSLF
Sbjct: 194 GSNLEGKGSYLAYFSEGLGKSADWETIMKYQRKNGSLF 231


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  107 bits (268), Expect(2) = 1e-29
 Identities = 49/75 (65%), Positives = 60/75 (80%)
 Frame = -2

Query: 226 SPSTTVAALAKLQDINCLNYLR*LLEKSGNAVPAAYPQDIYTELCMVDSLERLGMDRHFK 47
           SPS T AAL+ LQ+  CLNYLR LLEK GNAVP  YP D+Y  LC+VD+LERLG+DR+F+
Sbjct: 275 SPSATAAALSHLQNAGCLNYLRSLLEKFGNAVPTVYPLDLYARLCLVDNLERLGIDRYFR 334

Query: 46  NKIRNVLERTYSCWL 2
            +IR+VL+ TY CWL
Sbjct: 335 MEIRSVLDETYRCWL 349



 Score = 47.0 bits (110), Expect(2) = 1e-29
 Identities = 19/37 (51%), Positives = 28/37 (75%)
 Frame = -3

Query: 342 GSNSEGGNLYLAYVAEGLGKSQNWKDIWKLQKKNGSL 232
           GSN++    YLAY++EG+G+ Q+W+ + K Q KNGSL
Sbjct: 236 GSNTKSREAYLAYISEGMGRLQDWEMVMKYQMKNGSL 272


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
           vinifera]
          Length = 793

 Score =  107 bits (268), Expect(2) = 1e-29
 Identities = 49/75 (65%), Positives = 60/75 (80%)
 Frame = -2

Query: 226 SPSTTVAALAKLQDINCLNYLR*LLEKSGNAVPAAYPQDIYTELCMVDSLERLGMDRHFK 47
           SPS T AAL+ LQ+  CLNYLR LLEK GNAVP  YP D+Y  LC+VD+LERLG+DR+F+
Sbjct: 250 SPSATAAALSHLQNAGCLNYLRSLLEKFGNAVPTVYPLDLYARLCLVDNLERLGIDRYFR 309

Query: 46  NKIRNVLERTYSCWL 2
            +IR+VL+ TY CWL
Sbjct: 310 MEIRSVLDETYRCWL 324



 Score = 47.0 bits (110), Expect(2) = 1e-29
 Identities = 19/37 (51%), Positives = 28/37 (75%)
 Frame = -3

Query: 342 GSNSEGGNLYLAYVAEGLGKSQNWKDIWKLQKKNGSL 232
           GSN++    YLAY++EG+G+ Q+W+ + K Q KNGSL
Sbjct: 211 GSNTKSREAYLAYISEGMGRLQDWEMVMKYQMKNGSL 247


>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
           communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
           synthase B, chloroplast precursor, putative [Ricinus
           communis]
          Length = 808

 Score = 94.7 bits (234), Expect(2) = 6e-29
 Identities = 44/75 (58%), Positives = 57/75 (76%)
 Frame = -2

Query: 226 SPSTTVAALAKLQDINCLNYLR*LLEKSGNAVPAAYPQDIYTELCMVDSLERLGMDRHFK 47
           SPSTT AA + L++ +CL YL+ +L+K GNAVP  YP D+Y+ L MVD LERLG+DRHF+
Sbjct: 188 SPSTTAAAFSHLRNADCLQYLQSVLQKYGNAVPTIYPLDVYSRLLMVDILERLGIDRHFR 247

Query: 46  NKIRNVLERTYSCWL 2
            +I+ VLE TY  WL
Sbjct: 248 KEIKLVLEETYRYWL 262



 Score = 57.8 bits (138), Expect(2) = 6e-29
 Identities = 30/57 (52%), Positives = 41/57 (71%), Gaps = 1/57 (1%)
 Frame = -3

Query: 396 SMLKSR-VNIASGTPSRCSGSNSEGGNLYLAYVAEGLGKSQNWKDIWKLQKKNGSLF 229
           +ML  R V++ SG+     GSN+EG   YLAY+AEG+GK Q+W+ + K Q+KNGSLF
Sbjct: 134 AMLHRRDVDLKSGS----GGSNTEGRKAYLAYIAEGIGKFQDWEMVMKYQRKNGSLF 186


>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
          Length = 737

 Score = 91.7 bits (226), Expect(2) = 5e-27
 Identities = 44/75 (58%), Positives = 53/75 (70%)
 Frame = -2

Query: 226 SPSTTVAALAKLQDINCLNYLR*LLEKSGNAVPAAYPQDIYTELCMVDSLERLGMDRHFK 47
           SPSTT +A   L++ +CL YLR LLEK GNAVP  YP D Y  L M  SLE LG+DRHF+
Sbjct: 187 SPSTTASAFTHLKNADCLKYLRSLLEKFGNAVPTVYPLDKYARLSMAASLENLGIDRHFR 246

Query: 46  NKIRNVLERTYSCWL 2
            +IR+VL+ TY  WL
Sbjct: 247 EEIRSVLDETYRGWL 261



 Score = 54.3 bits (129), Expect(2) = 5e-27
 Identities = 29/69 (42%), Positives = 39/69 (56%), Gaps = 13/69 (18%)
 Frame = -3

Query: 396 SMLKSRVNIASGTPS-------------RCSGSNSEGGNLYLAYVAEGLGKSQNWKDIWK 256
           S L  ++N+  G P+             R   SNSEG   +LAY++EG GKSQ+W  + K
Sbjct: 117 SALNLKMNLPLGAPTLDALFHRRDFELKRGYASNSEGWRTFLAYISEGFGKSQDWDLVMK 176

Query: 255 LQKKNGSLF 229
            Q+KNGSLF
Sbjct: 177 YQRKNGSLF 185