BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00016325
         (640 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel prot...    99   5e-19
ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel prot...    99   7e-19
ref|XP_003592677.1| mscS family protein, putative [Medicago trun...    86   6e-15
ref|XP_004144925.1| PREDICTED: mechanosensitive ion channel prot...    81   1e-13
ref|XP_002889201.1| mechanosensitive ion channel domain-containi...    80   3e-13

>ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score = 99.4 bits (246), Expect = 5e-19
 Identities = 81/233 (34%), Positives = 114/233 (48%), Gaps = 27/233 (11%)
 Frame = +2

Query: 14  SFKQVQTKISGNGVQHQHQKLESQPILSDLDD---LKCESSGRRDIVLNIN-SDVEVSRE 181
           S+K V+ K+SG      H+ L   PILSD         +SS R+++++ ++ +D    R 
Sbjct: 13  SYKYVR-KLSGADATQDHEHL---PILSDHQHSIMAVSDSSDRKEVIVKVDEADASTLRA 68

Query: 182 VDNNNNNSGSTIWRGSSYDFWSDHXXXXXXXXXVVAGNSSNEDGFDFKNN--NVEDPPSK 355
            D  N +   TIWR SSYDFW+D             G +   D F+F+ +  +VEDPPSK
Sbjct: 69  TDLVNGSG--TIWRESSYDFWNDSDNRRNYGE----GGAKTTDDFEFRQHRKDVEDPPSK 122

Query: 356 LIGKFLHRQKASGEIAXXXXXXXXXXSEAVVGNSGLQAIQEVPF--SPKEAKVSFR---E 520
           LIG+FLH+QKASGE++           E     + L  + E P   S +E KVSF    E
Sbjct: 123 LIGQFLHKQKASGEMSLDMDMEML---ELPQDKTPLSTVAESPMRRSSRELKVSFESISE 179

Query: 521 QGENWS----------------PSPEAVHRRSNGSVPEEGRGGEVLRCTSNNA 631
             EN S                  P    RR++GS  E+    EVLRC+SN++
Sbjct: 180 ISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSS 232


>ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score = 99.0 bits (245), Expect = 7e-19
 Identities = 81/232 (34%), Positives = 113/232 (48%), Gaps = 27/232 (11%)
 Frame = +2

Query: 14  SFKQVQTKISGNGVQHQHQKLESQPILSDLDD---LKCESSGRRDIVLNIN-SDVEVSRE 181
           S+K V+ K+SG      H+ L   PILSD         +SS R+++++ ++ +D    R 
Sbjct: 13  SYKYVR-KLSGADATQDHEHL---PILSDHQHSIMAVSDSSDRKEVIVKVDEADASTLRA 68

Query: 182 VDNNNNNSGSTIWRGSSYDFWSDHXXXXXXXXXVVAGNSSNEDGFDFKNN--NVEDPPSK 355
            D  N +   TIWR SSYDFW+D             G +   D F+F+ +  +VEDPPSK
Sbjct: 69  TDLVNGSG--TIWRESSYDFWNDSDNRRNYGE----GGAKTTDDFEFRQHRKDVEDPPSK 122

Query: 356 LIGKFLHRQKASGEIAXXXXXXXXXXSEAVVGNSGLQAIQEVPF--SPKEAKVSFR---E 520
           LIG+FLH+QKASGE++           E     + L  + E P   S +E KVSF    E
Sbjct: 123 LIGQFLHKQKASGEMSLDMDMEML---ELPQDKTPLSTVAESPMRRSSRELKVSFESISE 179

Query: 521 QGENWS----------------PSPEAVHRRSNGSVPEEGRGGEVLRCTSNN 628
             EN S                  P    RR++GS  E+    EVLRC+SN+
Sbjct: 180 ISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNS 231


>ref|XP_003592677.1| mscS family protein, putative [Medicago truncatula]
           gi|355481725|gb|AES62928.1| mscS family protein,
           putative [Medicago truncatula]
          Length = 926

 Score = 85.9 bits (211), Expect = 6e-15
 Identities = 78/248 (31%), Positives = 107/248 (43%), Gaps = 35/248 (14%)
 Frame = +2

Query: 2   SLKKSFKQVQTKISGNG----VQHQHQKLESQPILSDLDDLKCESSGRRDIVLNINSDVE 169
           ++KKSFK   ++ + +         H++L   PIL D            D+V+ IN D  
Sbjct: 3   TIKKSFKSYASQNNKHSRKFSADSDHEQL---PILHDRQHSSRSFPMAGDVVVKINDDGG 59

Query: 170 VSREVDNNNNNSGSTIWRGSSYDFWSDHXXXXXXXXXVVAGNSSNEDGFDFKNN-NVEDP 346
              + +N        IWR SSYDFW+D             G++  ++ FDF+N   +EDP
Sbjct: 60  EPSQANNK-------IWRESSYDFWND------------TGDNVRDESFDFRNKAKLEDP 100

Query: 347 PSKLIGKFLHRQKASGEIAXXXXXXXXXXSEAVVGNSG-LQAIQEVP-FSPKEAKVSFRE 520
           PS+LIGKFLH+Q+ASG++                G  G L  ++E P    +E KVSF E
Sbjct: 101 PSQLIGKFLHKQRASGDMLLDMDLEMEELQNEGNGADGKLTPVEESPTVIQRELKVSFEE 160

Query: 521 QGENW------------------SPS----------PEAVHRRSNGSVPEEGRGGEVLRC 616
              N                   SPS          P    RRS       G   EVLRC
Sbjct: 161 PASNSNGIDAVVNDPVRRRHSKDSPSMGEYARPPQPPNHDRRRSPSPAGHGGGDCEVLRC 220

Query: 617 TSNNASFK 640
            S+NASF+
Sbjct: 221 -SSNASFE 227


>ref|XP_004144925.1| PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis
           sativus] gi|449529323|ref|XP_004171649.1| PREDICTED:
           mechanosensitive ion channel protein 6-like [Cucumis
           sativus]
          Length = 955

 Score = 81.3 bits (199), Expect = 1e-13
 Identities = 69/230 (30%), Positives = 110/230 (47%), Gaps = 29/230 (12%)
 Frame = +2

Query: 2   SLKKSFK---------QVQTKISGNGVQHQ-------HQKLESQPILSDLDDLK-CESSG 130
           +LKKSFK         ++    +G+ + H+       HQ  +    L D   +   ++S 
Sbjct: 3   TLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDTSD 62

Query: 131 RRDIVLNIN---SDVEVSREVDNNNNNSGSTIWRGSSYDFWSDHXXXXXXXXXVVAGNSS 301
           R +++L I+   S   VSR +D+  NN G+ +WR S YDFW++          +  G++S
Sbjct: 63  RTEVILKIDGGGSSSAVSRSLDSAGNNGGN-VWRESRYDFWNNDEIGIGESARM--GDNS 119

Query: 302 NED---GFDFKNNN--VEDPPSKLIGKFLHRQKASGEIAXXXXXXXXXXSEAVVGNSGLQ 466
             D   GF+F      +EDPP+KLIG FL +QK  GE             E +  N  + 
Sbjct: 120 GVDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETT----LDMDLEMEELKPNRIVP 175

Query: 467 AIQEVPFS--PKEAKVSFREQGENWSPSPEAVHRRSNGS--VPEEGRGGE 604
            + E P S   K+ KVSF+      S + +++ RR+  S  + EE +GG+
Sbjct: 176 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQ 225


>ref|XP_002889201.1| mechanosensitive ion channel domain-containing protein [Arabidopsis
           lyrata subsp. lyrata] gi|297335042|gb|EFH65460.1|
           mechanosensitive ion channel domain-containing protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 857

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 60/183 (32%), Positives = 87/183 (47%), Gaps = 14/183 (7%)
 Frame = +2

Query: 119 ESSGRRDIVLNINSDVEVSREVDNNNNNSGST---IWRGSSYDFWSDHXXXXXXXXXVVA 289
           +S+ RR++++ I+ D         NN  SG T   IWR  SYDFW+D            A
Sbjct: 4   DSADRREVIVKIDGD---------NNGVSGETVGRIWRDGSYDFWTDGEGNLNKGHNAAA 54

Query: 290 GNSSN---------EDGFDFKNNNVEDPPSKLIGKFLHRQKASGEIAXXXXXXXXXXSEA 442
            +S           ++GF+F+    EDPP+KLIG+FLH+Q+ASGEI            + 
Sbjct: 55  VDSDRSAATTEEQQDEGFEFRRG--EDPPTKLIGQFLHKQQASGEICL----------DM 102

Query: 443 VVGNSGLQA--IQEVPFSPKEAKVSFREQGENWSPSPEAVHRRSNGSVPEEGRGGEVLRC 616
            +G   LQ+  +  V  SP  AKV         +  P+ V RR + S       GEV++C
Sbjct: 103 DLGMDELQSRGLTPVSESPTSAKVP--------TKPPDPVGRRDSRSNNNNNDDGEVVKC 154

Query: 617 TSN 625
           + N
Sbjct: 155 SGN 157