BLASTX nr result
ID: Coptis25_contig00015922
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00015922
(1345 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002274489.2| PREDICTED: LOW QUALITY PROTEIN: midasin-like... 310 3e-95
emb|CBI35900.3| unnamed protein product [Vitis vinifera] 301 6e-93
ref|XP_002327931.1| predicted protein [Populus trichocarpa] gi|2... 239 1e-72
ref|XP_004141809.1| PREDICTED: midasin-like [Cucumis sativus] 210 5e-64
ref|XP_004155970.1| PREDICTED: LOW QUALITY PROTEIN: midasin-like... 206 8e-63
>ref|XP_002274489.2| PREDICTED: LOW QUALITY PROTEIN: midasin-like [Vitis vinifera]
Length = 5286
Score = 310 bits (794), Expect(2) = 3e-95
Identities = 169/330 (51%), Positives = 224/330 (67%), Gaps = 2/330 (0%)
Frame = +3
Query: 357 LESCGLSRHRSMSSEDAHGSNQPTSWFLQPSYNMQHLLVPPNGNCSI--VDATCNSQSLK 530
LESCGLSRH+S+ ED SNQ +SW LQPSY++QHLL P G VD +SQ
Sbjct: 3953 LESCGLSRHKSIFFEDQLKSNQ-SSWLLQPSYDVQHLL-PMQGRLPYQNVDLAASSQLQS 4010
Query: 531 PLPNNFDSSWIAANHFYYSSMAMVHLLRKICLNFHKDISLDQVTRSVSFLEHLIIIQQEQ 710
+ D W AAN +Y+ S+A V LLR+ICLNFHKD +L+QV RSVSFL+HLIIIQQEQ
Sbjct: 4011 LIHEVSDVEWSAANRYYFKSIASVQLLRQICLNFHKDFTLEQVNRSVSFLDHLIIIQQEQ 4070
Query: 711 RSVAYKLSEDIQNLRGSLNSLNDFHTNPSFTDHVTTAESLVSLKQHATRKCMWKQKKLFD 890
R+ Y SE +++LR S+ SL + +++ + D+ T ++ V+ QHAT K MW+QK+LFD
Sbjct: 4071 RNAVYHFSEHVKDLRKSVASLENLYSSSTTCDNGTDSKCSVAPNQHATLKYMWQQKQLFD 4130
Query: 891 SLYALSHETSLFLRKVEVTHLNTCQSVKFEANEVLACIEKLLPNIRKSKESLDQYLLGGN 1070
L ++ HE SL LR VE THL+TCQ VK AN VL IEK +P +KSKESLD YLLG N
Sbjct: 4131 GLCSMLHEESLLLRTVESTHLSTCQHVKGSANRVLVFIEKFVPLFQKSKESLDDYLLGRN 4190
Query: 1071 KILTTPEAFSLPFIVSKPMEQLVMQNFVVLSELEENIQAFTKHDVDKRSVKEPLIGHFLD 1250
++LTT P +++K MEQLV QNF V+ E EE + AF + +VD+RSV+E L+ F D
Sbjct: 4191 RVLTTVATSFYPPVITKQMEQLVFQNFQVIREFEECLCAFQRQEVDRRSVQEVLLNRFED 4250
Query: 1251 LLNKGKATMQEFCSFLEPIQDISVPDNDAS 1340
++ KGKA ++F + LE ++S D + S
Sbjct: 4251 IMKKGKAMAEQFNNALEGRSELSPCDENHS 4280
Score = 66.6 bits (161), Expect(2) = 3e-95
Identities = 31/54 (57%), Positives = 40/54 (74%)
Frame = +2
Query: 95 LSSQSTCLMYSEEWSKLWFSIEKIFRRVTECADLWEIEKKNFGKRRALSDLLKL 256
L+S S CL+Y E W ++ ++E + R VTECADLW+ KN GKRRALS+LLKL
Sbjct: 3899 LASPSPCLVYLEHWREVRSTLEHVCRTVTECADLWKDVSKNLGKRRALSELLKL 3952
>emb|CBI35900.3| unnamed protein product [Vitis vinifera]
Length = 5267
Score = 301 bits (771), Expect(2) = 6e-93
Identities = 164/330 (49%), Positives = 218/330 (66%), Gaps = 2/330 (0%)
Frame = +3
Query: 357 LESCGLSRHRSMSSEDAHGSNQPTSWFLQPSYNMQHLLVPPNGNCSI--VDATCNSQSLK 530
LESCGLSRH+S+ E W LQPSY++QHLL P G VD +SQ
Sbjct: 3953 LESCGLSRHKSIFFEVLKNECYFLCWLLQPSYDVQHLL-PMQGRLPYQNVDLAASSQLQS 4011
Query: 531 PLPNNFDSSWIAANHFYYSSMAMVHLLRKICLNFHKDISLDQVTRSVSFLEHLIIIQQEQ 710
+ D W AAN +Y+ S+A V LLR+ICLNFHKD +L+QV RSVSFL+HLIIIQQEQ
Sbjct: 4012 LIHEVSDVEWSAANRYYFKSIASVQLLRQICLNFHKDFTLEQVNRSVSFLDHLIIIQQEQ 4071
Query: 711 RSVAYKLSEDIQNLRGSLNSLNDFHTNPSFTDHVTTAESLVSLKQHATRKCMWKQKKLFD 890
R+ Y SE +++LR S+ SL + +++ + D+ T ++ V+ QHAT K MW+QK+LFD
Sbjct: 4072 RNAVYHFSEHVKDLRKSVASLENLYSSSTTCDNGTDSKCSVAPNQHATLKYMWQQKQLFD 4131
Query: 891 SLYALSHETSLFLRKVEVTHLNTCQSVKFEANEVLACIEKLLPNIRKSKESLDQYLLGGN 1070
L ++ HE SL LR VE THL+TCQ VK AN VL IEK +P +KSKESLD YLLG N
Sbjct: 4132 GLCSMLHEESLLLRTVESTHLSTCQHVKGSANRVLVFIEKFVPLFQKSKESLDDYLLGRN 4191
Query: 1071 KILTTPEAFSLPFIVSKPMEQLVMQNFVVLSELEENIQAFTKHDVDKRSVKEPLIGHFLD 1250
++LTT P +++K MEQLV QNF V+ E EE + AF + +VD+RSV+E L+ F D
Sbjct: 4192 RVLTTVATSFYPPVITKQMEQLVFQNFQVIREFEECLCAFQRQEVDRRSVQEVLLNRFED 4251
Query: 1251 LLNKGKATMQEFCSFLEPIQDISVPDNDAS 1340
++ KGKA ++F + LE ++S D + S
Sbjct: 4252 IMKKGKAMAEQFNNALEGRSELSPCDENHS 4281
Score = 67.8 bits (164), Expect(2) = 6e-93
Identities = 32/54 (59%), Positives = 40/54 (74%)
Frame = +2
Query: 95 LSSQSTCLMYSEEWSKLWFSIEKIFRRVTECADLWEIEKKNFGKRRALSDLLKL 256
LSS S CL+Y E W ++ ++E + R VTECADLW+ KN GKRRALS+LLKL
Sbjct: 3899 LSSPSPCLVYLEHWREVRSTLEHVCRTVTECADLWKDVSKNLGKRRALSELLKL 3952
>ref|XP_002327931.1| predicted protein [Populus trichocarpa] gi|222837340|gb|EEE75719.1|
predicted protein [Populus trichocarpa]
Length = 5317
Score = 239 bits (609), Expect(2) = 1e-72
Identities = 135/312 (43%), Positives = 193/312 (61%), Gaps = 2/312 (0%)
Frame = +3
Query: 357 LESCGLSRHRSMSSEDAHGSNQPTSW-FLQPSYNMQHLLVPPNGNCSIVDATCNSQSLKP 533
L++ GL +H+ + ++ SN W F+QPSYN QHLL+ P+ S L+
Sbjct: 4000 LDTSGLHKHKFEIMKISNSSN----WLFIQPSYNAQHLLLTPSRLSGEAFDVSTSSELQC 4055
Query: 534 LPNNF-DSSWIAANHFYYSSMAMVHLLRKICLNFHKDISLDQVTRSVSFLEHLIIIQQEQ 710
LP+++ D+ W +AN FY+ S+A V +++ICL H DI+ DQ +R+VSFL HLIIIQQ Q
Sbjct: 4056 LPDDYVDTMWKSANEFYFKSVASVQFMQRICLKPHGDITYDQASRAVSFLNHLIIIQQSQ 4115
Query: 711 RSVAYKLSEDIQNLRGSLNSLNDFHTNPSFTDHVTTAESLVSLKQHATRKCMWKQKKLFD 890
R+ AY S+ ++ LR + + + + D T++E + QHA +CMWKQK+LFD
Sbjct: 4116 RAAAYGFSKQLKCLRECTYAFENSYVKCTDIDERTSSEYSIVQNQHAFFQCMWKQKQLFD 4175
Query: 891 SLYALSHETSLFLRKVEVTHLNTCQSVKFEANEVLACIEKLLPNIRKSKESLDQYLLGGN 1070
L L E SL LR VE THL +C+SV+ AN VL IEK +P +KSKESLD+ LLG
Sbjct: 4176 GLTTLLAEESLLLRTVESTHLKSCRSVRPAANHVLQFIEKFIPVTQKSKESLDKSLLG-- 4233
Query: 1071 KILTTPEAFSLPFIVSKPMEQLVMQNFVVLSELEENIQAFTKHDVDKRSVKEPLIGHFLD 1250
+++T S P+I+SK MEQLV +NF V+ E EE+ F K D ++ + E L+GHF D
Sbjct: 4234 RVVTISAGPSRPYIISKQMEQLVYKNFQVIKEFEEHFFDFRKQDWNRSFIIETLLGHFDD 4293
Query: 1251 LLNKGKATMQEF 1286
+ +GK +F
Sbjct: 4294 VFKEGKMLADQF 4305
Score = 62.4 bits (150), Expect(2) = 1e-72
Identities = 31/70 (44%), Positives = 43/70 (61%)
Frame = +2
Query: 47 LSLKDAEDTEGFIRLSLSSQSTCLMYSEEWSKLWFSIEKIFRRVTECADLWEIEKKNFGK 226
LS D +D R L+S S+ L E+W+ L +++EKIF+ +C DLW K GK
Sbjct: 3930 LSFLDNKDVTSITRQCLASHSSHLSRDEQWNVLCWTVEKIFKTAMDCDDLWNDTGKGVGK 3989
Query: 227 RRALSDLLKL 256
+RALS+LLKL
Sbjct: 3990 KRALSELLKL 3999
>ref|XP_004141809.1| PREDICTED: midasin-like [Cucumis sativus]
Length = 5062
Score = 210 bits (535), Expect(2) = 5e-64
Identities = 115/326 (35%), Positives = 192/326 (58%), Gaps = 1/326 (0%)
Frame = +3
Query: 357 LESCGLSRHRSMSSEDAHGSNQPTSWFLQPSYNMQHLLVPPNGNCSIVDATCNSQSLKPL 536
LE+ GLSRH+S+ E+ N+ + WFLQ S ++Q+LL+ + V S +K
Sbjct: 3737 LETSGLSRHKSIYLEE----NRKSWWFLQQSDDIQYLLLSQS-RLRGVSVDTPSSDVKEF 3791
Query: 537 PN-NFDSSWIAANHFYYSSMAMVHLLRKICLNFHKDISLDQVTRSVSFLEHLIIIQQEQR 713
N + A +Y+ S+ V LL++ CLN HKD++ +QV RS SFL LI+IQQ+Q
Sbjct: 3792 QNLGLGAKRSEAIEYYFKSVKAVLLLQQTCLNSHKDVTREQVERSCSFLNQLIVIQQKQH 3851
Query: 714 SVAYKLSEDIQNLRGSLNSLNDFHTNPSFTDHVTTAESLVSLKQHATRKCMWKQKKLFDS 893
+ A ++ + +LR ++ L +++ + ES +S Q +CMW+QKK+FDS
Sbjct: 3852 TAADHFAKHLNHLRSCVSLLEKLYSSSKDSSARNGYESRISCNQEIIYRCMWQQKKIFDS 3911
Query: 894 LYALSHETSLFLRKVEVTHLNTCQSVKFEANEVLACIEKLLPNIRKSKESLDQYLLGGNK 1073
L ++ E + L+ + HL +C+S+K E + ++ IE LP +KSKE LD YLLG +
Sbjct: 3912 LNTMAQEELILLKSFKNVHLKSCRSIKSEEHWIIEAIETYLPGFQKSKECLDNYLLGQKE 3971
Query: 1074 ILTTPEAFSLPFIVSKPMEQLVMQNFVVLSELEENIQAFTKHDVDKRSVKEPLIGHFLDL 1253
+++TP + P++V++ M++LV QNF V++ +E++ +K ++RS++ L+GHF ++
Sbjct: 3972 VISTPASILQPYVVTEQMKELVSQNFEVINIFKEHLSTLSKRVANQRSIENILLGHFDEV 4031
Query: 1254 LNKGKATMQEFCSFLEPIQDISVPDN 1331
K +EF S LE + IS +N
Sbjct: 4032 FEKSSIVEEEFRSGLEAVSSISNGEN 4057
Score = 62.0 bits (149), Expect(2) = 5e-64
Identities = 37/83 (44%), Positives = 48/83 (57%), Gaps = 5/83 (6%)
Frame = +2
Query: 23 VSLMGLP-----YLSLKDAEDTEGFIRLSLSSQSTCLMYSEEWSKLWFSIEKIFRRVTEC 187
V L G P + SLK ED I+ SQS L Y EEW L ++E+I++R C
Sbjct: 3655 VCLNGTPEYKRSFSSLKSVEDLISVIQQQ-ESQSQSLSYQEEWKSLSCTVERIYQRACYC 3713
Query: 188 ADLWEIEKKNFGKRRALSDLLKL 256
+W+ KK+ GKRRALS+LLKL
Sbjct: 3714 DVIWKESKKSQGKRRALSELLKL 3736
>ref|XP_004155970.1| PREDICTED: LOW QUALITY PROTEIN: midasin-like [Cucumis sativus]
Length = 5431
Score = 206 bits (525), Expect(2) = 8e-63
Identities = 113/326 (34%), Positives = 191/326 (58%), Gaps = 1/326 (0%)
Frame = +3
Query: 357 LESCGLSRHRSMSSEDAHGSNQPTSWFLQPSYNMQHLLVPPNGNCSIVDATCNSQSLKPL 536
LE+ GLSRH+S+ E+ N+ + WFLQ S ++Q+LL+ + V S +K
Sbjct: 4106 LETSGLSRHKSIYLEE----NRKSWWFLQQSNDIQYLLLSQS-RLRGVSVDTPSSDVKEF 4160
Query: 537 PN-NFDSSWIAANHFYYSSMAMVHLLRKICLNFHKDISLDQVTRSVSFLEHLIIIQQEQR 713
N + A +Y+ S+ V LL++ CLN HKD++ +QV RS SFL LI+IQQ+Q
Sbjct: 4161 QNLGLGAKRSEAIEYYFKSVKAVLLLQQTCLNSHKDVTREQVERSCSFLNQLIVIQQKQH 4220
Query: 714 SVAYKLSEDIQNLRGSLNSLNDFHTNPSFTDHVTTAESLVSLKQHATRKCMWKQKKLFDS 893
+ A ++ + +L+ ++ L +++ + ES +S Q +CMW+QKK+FDS
Sbjct: 4221 TAADHFAKHLNHLKSCVSLLEKLYSSSKDSSARNGYESRISCNQEIIYRCMWQQKKIFDS 4280
Query: 894 LYALSHETSLFLRKVEVTHLNTCQSVKFEANEVLACIEKLLPNIRKSKESLDQYLLGGNK 1073
L ++ E + L+ + HL +C+S+K E + ++ IE LP +KSKE LD YLLG +
Sbjct: 4281 LNTMAQEELILLKSFKNVHLKSCRSIKSEEHCIIEAIETYLPGFQKSKECLDNYLLGQKE 4340
Query: 1074 ILTTPEAFSLPFIVSKPMEQLVMQNFVVLSELEENIQAFTKHDVDKRSVKEPLIGHFLDL 1253
+++TP + P++V++ M++LV QN V++ +E++ +K ++RS++ L+GHF ++
Sbjct: 4341 VISTPASILQPYVVTEQMKELVSQNVEVINIFKEHLSTLSKRVANQRSIENILLGHFDEV 4400
Query: 1254 LNKGKATMQEFCSFLEPIQDISVPDN 1331
K +EF S LE + IS +N
Sbjct: 4401 FEKSSIVEEEFRSGLEAVSSISNGEN 4426
Score = 62.0 bits (149), Expect(2) = 8e-63
Identities = 37/83 (44%), Positives = 48/83 (57%), Gaps = 5/83 (6%)
Frame = +2
Query: 23 VSLMGLP-----YLSLKDAEDTEGFIRLSLSSQSTCLMYSEEWSKLWFSIEKIFRRVTEC 187
V L G P + SLK ED I+ SQS L Y EEW L ++E+I++R C
Sbjct: 4024 VCLNGTPEYKRSFSSLKSVEDLISVIQQQ-ESQSQSLSYQEEWKSLSCTVERIYQRACYC 4082
Query: 188 ADLWEIEKKNFGKRRALSDLLKL 256
+W+ KK+ GKRRALS+LLKL
Sbjct: 4083 DVIWKESKKSQGKRRALSELLKL 4105